EN ISO 8692-2012 en Water quality - Fresh water algal growth inhibition test with unicellular green algae《水质 用单细胞绿藻进行淡水藻类生长抑制性试验》.pdf
《EN ISO 8692-2012 en Water quality - Fresh water algal growth inhibition test with unicellular green algae《水质 用单细胞绿藻进行淡水藻类生长抑制性试验》.pdf》由会员分享,可在线阅读,更多相关《EN ISO 8692-2012 en Water quality - Fresh water algal growth inhibition test with unicellular green algae《水质 用单细胞绿藻进行淡水藻类生长抑制性试验》.pdf(30页珍藏版)》请在麦多课文档分享上搜索。
1、raising standards worldwideNO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBSI Standards PublicationBS EN ISO 8692:2012Water quality Fresh wateralgal growth inhibition testwith unicellular green algae(ISO 8692:2012)BS EN ISO 8692:2012 BRITISH STANDARDNational forewordThis Briti
2、sh Standard is the UK implementation of EN ISO 8692:2012. It supersedes BS EN ISO 8692:2004, which is withdrawn.The UK participation in its preparation was entrusted to T e c h n i c a l C o m m i t t e e E H / 3 / 5 , B i o l o g i c a l M e t h o d s .A list of organizations represented on this co
3、mmittee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2012Published by BSI Standards Limited 2012ISBN 978 0 580 68532 3 ICS 13.0
4、60.70 Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 March 2012.Amendments issued since publicationDate T e x t a f f e c t e dEUROPEAN STANDARD NORME EUROPE
5、NNE EUROPISCHE NORM EN ISO 8692 February 2012 ICS 13.060.70 Supersedes EN ISO 8692:2004English Version Water quality - Fresh water algal growth inhibition test with unicellular green algae (ISO 8692:2012) Qualit de leau - Essai dinhibition de la croissance des algues deau douce avec des algues verte
6、s unicellulaires (ISO 8692:2012) Wasserbeschaffenheit - Swasseralgen-Wachstumshemmtest mit einzelligen Grnalgen (ISO 8692:2012) This European Standard was approved by CEN on 14 February 2012. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for
7、 giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three
8、 official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodi
9、es of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and Un
10、ited Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2012 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 8692:2
11、012: EBS EN ISO 8692:2012 EN ISO 8692:2012 (E) 3 Foreword This document (EN ISO 8692:2012) has been prepared by Technical Committee ISO/TC 147 “Water quality” in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN. This European Standard shall b
12、e given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by August 2012, and conflicting national standards shall be withdrawn at the latest by August 2012. Attention is drawn to the possibility that some of the elements of this document
13、may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 8692:2004. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound
14、 to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sw
15、eden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 8692:2012 has been approved by CEN as a EN ISO 8692:2012 without any modification. BS EN ISO 8692:2012ISO 8692:2012(E) ISO 2012 All rights reserved iiiContents PageForeword iv1 Scope 12 Normative references . 13 Ter
16、ms and definitions . 14 Principle . 25 Reagents and media . 26 Apparatus 47 Procedure 57.1 Preparation of growth medium 57.2 Preparation of preculture and inoculum 57.3 Choice of test sample concentrations . 57.4 Preparation of test sample and stock solutions . 57.5 Preparation of test and control b
17、atches . 67.6 Incubation 67.7 Measurements 78 Validity criteria . 79 Calculation 79.1 Plotting of growth curves 79.2 Calculation of percentage inhibition 89.3 Determination of ErCx(e.g. ErC10and ErC50) . 810 Expression of results . 811 Interpretation of results . 912 Precision 913 Test report . 9Ann
18、ex A (informative) Rapid screening of waste water algal growth inhibition 11Annex B (informative) Test procedure with algae from algal beads, with direct measurement of algal growth in spectrophotometric cells .14Annex C (informative) Procedure for immobilization of algae in alginate beads 19Bibliog
19、raphy .21BS EN ISO 8692:2012ISO 8692:2012(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member
20、 body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Elect
21、rotechnical Commission (IEC) on all matters of electrotechnical standardization.International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.The main task of technical committees is to prepare International Standards. Draft International Standards adopted
22、by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.Attention is drawn to the possibility that some of the elements of this document may be the subject of patent ri
23、ghts. ISO shall not be held responsible for identifying any or all such patent rights.ISO 8692 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods.This third edition cancels and replaces the second edition (ISO 8692:2004), which has been technically r
24、evised.iv ISO 2012 All rights reservedBS EN ISO 8692:2012INTERNATIONAL STANDARD ISO 8692:2012(E)Water quality Fresh water algal growth inhibition test with unicellular green algaeWARNING Persons using this International Standard should be familiar with normal laboratory practice. This International
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