1、DB61 1i f 1* DB61/T 413-2007 t I 2007-09-03 2007-09-03 DB611T 413-2007 *tiFftJ:f:llJ tA*,H1HjL *ftJ:f:lAMmA.m*acQ *ftN: J:f:lAmA.Q I *ftA: *WWQ *tjf,ftJktt;fpQ I I I i I I I ! i i !j I I ! , J l ; i I I DB61fT 413-2007 t f I t l 1 ;:$: tift/fUJI! 7liffWlulE11A*4* D-:3jZ *mUfJiitlr1Jo *fiftttE m mTiX
2、*4*D-1f:*itW!IJlEo 2 tJUiz31 iJ Imjc14 r:yux1 L I=* iJjffiJi*tittEiJ iJ! ).trnfi JjJ ;:$:t;j;ttEiJ,o JLM:r:E BjtJjiJ iJ! J+1 ft:, ;ltPiQfr JJi:tHi1 .a.(iJ).rrmmT*ttE,W,.a.M*ttEiJ* MEJ1ffli3:X11:iJ1iifA&;:$:o fLfikff BWHfJtrmfll:, ;ltiid#rA&;:$:JffJ:rFf;:$:flFttEo EN 12138: ;J.*UJlirtcpD-1ji:Il1ttiJl
3、ifW!1E . D-MDH D1jZ*llil1i1i:lll?tI. NAD :tj!JfiIi,*-A1rllil NADH f3 -:li!Iw:-nut-:i%tfllil U mIJ lF Jfl- f.fr., if 25C iJ fij It14: ftJ= 5 1 U iJ mit.$tf ft PX; 1 mol iJ0 A 11jAIiil1i1i:*11 .:ftfWt 0 D-MDH, dec arb J ) D-*1i1i: + NAD . p;jm11i1i: + CO:c + NADH + H NADH iJ , ffi D-1jL*!IiiJ , :!tit1
4、1: 0 I I NADHiJid:rn tE334, 340! 1.0em 6. 3 91*:7:;Jlit, r:iJSiJt*, tE 340nm JIJ:AE 6. 4 jJf:ll, 11 10 11m 6. 5 Ji IL,ll 6. 6 ILi tf, jtIL n;*ftG;, J.,*,j 190 mL:m:*r:p:5t!tlIJ:JJO/0.8 g NaCL 0.037 g KCL 0.0135 g NazHPO , 2H20, 0.1 g 1i1rfJ, 0.5 g hepes, 3EJhfftJfi, J.j:j NaOH pH 7.05, 100mlo 7.1.2
5、NAD I1-t:Hi ImL l1iJ*mflllf. 52. 5rng f1)f)P;k) D1ji:lti:lHl;:Jmtl.!W.I1t, -15C - -20C1*#o 7. 1.4 HCl: 2 mollL 7. 1. 5 NaOH: 2mollL 7.1.6 KOH: 2mollL 7.2 litt$ililji 7. 2. 1 1Ct;t J+j KOH llWl 25rnl m:H pH fll 78. :J.mm:l.JiJj.1im. 1lJ:H 1. 000 L 800 mL iJ11ft:i1I!uo 7. 2. 2 ;!ll=*5t*$IJtiJ1lJt:W 0
6、7.2.3 *CO 2 f*=*jtf$iJ11Tt:! 0 7. 3 f C:k11 20 :7t), IttIJfig 1.000mL 1.000mL 0.100mL 0.100mL - 0.100mL 1.800mL 1.700mL $iI. *1 6 :7tJw;IttIJJldffaa9IJfiWoJ9ta9mffl: giL I ;fimlHJ9tIJ*fFJ9t:l:o D-1fl*D1f:fFJ9t:l: 134.09 g/mo1, D1fl*D.!f:1f:fF MW 132.07g/mol VI !tlITI.*.m.IJ,. V 2 fflJffm.IJA, .a f f
7、fm.a9ffr d !tlITI.IJYt;f.!i!, J(!ffLa em e NADPHlJmYt: .:tE 340 nm := 6.3 1 x mmor 1 x em l 3 DB61rr 4132007 9 D-:!jfUmIJP-ttr.Jlitfi 9.1 DMm.-*tr.JD*m. 9. 2 tr.JJ2i:m 9.2. 1 ;tfi;fjQ)HfiIJE:=:ltJ (Omin), .OO(Ar)*W*1 A t ( *4t11Jfj:iF;) ;iJ:*J!q f ) 2, ff1litlS9fltitP, !WJtU:%TJ5IlffT 2C-SC. i1!ic1l
8、i 1 jpxS9 NAD IfttE 2C-8CPJ)E f r I =ffd, eX;tE-15C-25C*fttrPJ1*ff.=:1-Ji Q i1!iC1IitlnZS9 D-MDH ItFtE 2C8CPJ)E 5:R. i)(. I I I f j A:ffS9, m:*, :ffSmS9. 1 I I 5, tLj!j9JUJtU7tJJtflIt!HAi8/J (tLOO. 100), )JlIJ:ffmJ5Ilm:mr5ltJl, !PtE 7.3. 2 J)EJ:f:lilJ;lJo I 1 ! ! I I I I I I J t I , I I I I i I f , r , t t I ! i I ! , ! 5 I I
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