DB45 T 2311-2021 牛传染性鼻气管炎病毒的检测 巢式 聚合酶链反应法.pdf

1、g3 g3 g3 ICSg3g3g20g20g17 CCS g3g37g3g23 g5301 g10385g1366 g39g72g87g72g70g87 g21g19g21g20 g16 g21g21g19g3 g20g3 g16309 g3876 g7689g5725 g76g82g81g3g82g73g3g76 g19g23 g16 g21g24g2567 g3876g7173 g21873g8778 g81g73g72g70g87g76g82g88 g5177 g5301g16309g3876 g7173 g14368 g12759g9924 g18348 g86g3g69g82g89

2、g76g81g72 g70 g7173g14368g8945g2416 g8945 g3 g11259g8712 g19252g2563 g3g85g75g76g81g82g87g85 g75g68g76g81g3g85g72g68 g5176g3440g11527g11673 g2416 g11450g7926 g5322g8971 g68g70g75g72g76g87g76 g70g87g76g82g81g3 g12759g10812g4726 g3430 g39g37 g9089g3g3 g3 g86g3g89g76g85g88g86g258 g2567 g5177 45 g7151 g

3、7741 g23g24g18g55g3g21g22 g5144g5445 g49g72g86g87g72g71g3g83 g21g19g21g20 g16 g19 g3 g3 g7741g2044 g20g20g258g21g19g21g20 g3g3g3g3g3 g13968g2622 g82g79g92g80g72g85g68 g24 g16 g22g20g4564 g2044g3 g3 g3 g86g72g3 g7155 g39g37g23g24g18g55g3g21g22g20g20g252g21g19g21g20g3 g44g44g3 g2179g16438g3 g7516g7095

4、g1318g6457g10135g42g37g18g55g3g20g17g20g252g21g19g21g19g457g7735g2038g2374g5141g1420g4652g2121g3g3g12636g20g18200g2102g726g7735g2038g2374g7095g1318g11444g13571g7604g2748g17319g14713g16372g2121g458g11444g16372g4554 g17319g14713g452g3 g16935g8984g5951g7516g7095g1318g11444g7680g1227g1973g4582g2591g1412

5、5g9145g2554g1091g2137g452g7516g7095g1318g11444g2561g5171g7530g7604g1085g6319g6389g16886g2139g1091g2137g11444g17235g1323g452g3 g7516g7095g1318g11105g5295g16303g3870g7167g14362g8939g2410g1996g1098g1996g7553g2485g6656g2090g5286g4563g17247g452g3 g7516g7095g1318g11105g5295g16303g11148g10391g1098g7735g203

6、8g2374g6320g7519g4100g2696g1354g5506g2579g452g3 g7516g7095g1318g17319g14713g2437g1405g726g5295g16303g3870g7167g14362g8939g2410g1965g2411g11844g12454g6256g452g3 g7516g7095g1318g1131g16305g17319g14713g1258g726g14643g7332g451g5456g8769g12272g451g10683g11531g451g7550g4671g2068g451g7550g1129g451g1079g113

7、3g1995g451g19550g5639g2430g451g1323g13522g10681g452g3 g39g37g23g24g18g55g3g21g22g20g20g252g21g19g21g20g3 g20g3 g3 g10385g1366g7689g5725g21873g8778g12759g9924g11259g8712g11450g7926g9089g3g3g5144g5445g13968g2622g18348g19252g2563g5322g8971g3 g20g3 g14649g3370g3 g7516g7095g1318g16372g4554g1206g5138g5439

8、g13962g2616g18342g19246g2557g5316g8965g7920g9083g10379g1360g7683g5719g21867g8772g12753g9918g11253g8706g11444g7552g7113g2038g3895g451g6909g1420g7145g8965g2554g13571g7628g2132g4554g452g3 g7516g7095g1318g17970g11096g1214g5295g16303g15996g7023g2410g3599g1973g10379g1360g7683g5719g21867g8772g12753g9918g11

9、253g8706g11444g7920g9083g452g3 g21g3 g16378g14649g5725g5451g11102g7101g1324g3 g1083g2119g7095g1318g1117g11444g1973g4585g17994g17911g7095g1117g11444g16372g14643g5719g5445g11096g13884g7604g6208g7516g7095g1318g5621g1085g2591g4673g11444g7569g8558g452g1958g1117g712g8984g7189g7503g11444g5445g11096g7095g13

10、18g712 g1269g16917g7189g7503g4649g5316g11444g10360g7516g17970g11096g1214g7516g7095g1318g727g1085g8984g7189g7503g11444g5445g11096g7095g1318g712g1958g7472g7136g10360g7516g708g2357g6428g6256g7481g11444g1566g7017g2437g709g17970g11096g1214g7516 g7095g1318g452g3 g42g37g18g55g3g22g20g20g28g19g3g3g4558g2066

11、8g4564g5327g5427g2374g4502g2801g7014g19702g6320g7519g16372g14643g3 g22g3 g7525g16931g2754g4560g1151g3 g1083g2119g7519g16925g2748g4554g1145g17970g11096g1214g7516g7095g1318g452g3 g22g17g20g3 g3 g10385g1366g7689g5725g21873g8778g12759g9924g11259g8712g3g3g76g81g73g72g70g87g76g82g88g86g3g69g82g89g76g81g72

12、g3g85g75g76g81g82g87g85g68g70g75g72g76g87g76g86g3g89g76g85g88g86g3 g4750g11233g11241g11253g8706g12289g712g8804g11272g11253g8706g4750g712g3626g3344g13556g1130g2556g19246g39g49g36g452g3 g22g17g21g3 g3 g13968g2622g18348g19252g2563g5322g3g3g83g82g79g92g80g72g85g68g86g72g3g70g75g68g76g81g3g85g72g68g70g87

13、g76g82g81g3 g12720g12320g51g38g53g6320g7519g712g7263g1072g12285g11096g1214g7022g3927g6297g3790g10409g4554g11444g39g49g36g10359g8677g11444g2102g4480g11087g10393g4502g6320g7519g452g3 g22g17g22g3 g3 g5144g5445g51g38g53g2563g5322g3g3g81g72g86g87g72g71g3g83g82g79g92g80g72g85g68g86g72g3g70g75g68g76g81g3g8

14、5g72g68g70g87g76g82g81g3 g12720g12320g49g72g86g87g72g71g3g51g38g53g712g51g38g53g6320g7519g11444g1072g12285g10409g8634g5522g5439g712g3416g5224g16372g51g38g53g6297g3790g11444g10359g7133g1973g1981g16878g16849g1072g4649g7136g11444g5445g10393g712g4649g5224g16372 g51g38g53g11444g1239g10393g17931g15996g121

15、2g8529g51g38g53g6297g3790g712g6297g3790g2090g8708g5224g16372g51g38g53g7460g11805g11444g1239g10393g712g2591g7278g14983g6656g20744g1958g7920g9083g11444g7039g5967g5334g452g3 g22g17g23g3 g3 g5451g10399g3g3g83g85g76g80g72g85g86g3 g1086g5557g6297g3790g39g49g36g10359g8677g1108g1495g1218g16021g11444g1072g46

16、71g8677g4625g7784g14631g18344g452g3 g22g17g24g3 g3 g3916g3981g5451g10399g3g3g82g88g87g72g85g3g83g85g76g80g72g85g86g3 g11096g1214g12636g1072g8529g51g38g53g11444g5445g10393g452g3 g22g17g25g3 g3 g1979g3981g5451g10399g3g3g76g81g81g72g85g3g83g85g76g80g72g85g86g3 g11096g1214g12636g1212g8529g51g38g53g11444

17、g5445g10393g452g3 g23g3 g13663g11163g16931g3 g1083g2119g13657g11157g16925g17970g11096g1214g7516g7095g1318g452g3 g44g37g53g57g726g10379g1360g7683g5719g21867g8772g12753g9918g11253g8706g708g76g81g73g72g70g87g76g82g88g86g3g69g82g89g76g81g72g3g85g75g76g81g82g87g85g68g70g75g72g76g87g76g86g3g89g76g85g88g86

18、g709g3 g39g37g23g24g18g55g3g21g22g20g20g252g21g19g21g20g3 g21g3 g51g38g53g726g13962g2616g18342g19246g2557g5316g708g83g82g79g92g80g72g85g68g86g72g3g70g75g68g76g81g3g85g72g68g70g87g76g82g81g709g3 g24g3 g7558g7119g2044g3901g3 g24g17g20g3 g44g37g53g57g10415g5432g5725g5451g10399g3 g5445g10393g5311g2119g1

19、6369g19572g5509g36g1117g11444g36g17g20g17g20g452g3910g3975g5445g10393g41g20g2748g41g21g6297g3790g11444g11550g11444g10359g8677g1130g23g20g25g3g69g83g712g1973g3975g5445g10393g41g22g2748g41g23g6297g3790g11444g11550 g11444g10359g8677g1130g22g22g23 g3 g69g83g712g5445g10393g1455g11096g9091g5334g3447g1130g

20、24g19 g3 g83g80g82g79g18g173g47g712g13726g1214g16g21g19 g3 g263g1549g4488g452g3 g24g17g21g3 g16907g2168g3 g24g17g21g17g20g3 g39g49g36g6387g6662g16907g2168g3 g4018g1083g726g3 g252g252g3g55g85g76g93g82g79g3g39g49g36g6381g6656g16901g2162 g20g12 g11g6214g1958g4531g12665g7032g6381g6656g2162g12g727g3 g252

21、g252g3g8799g1327g727g3 g252g252g3g5426g1097g18359g727g3 g252g252g3g26g24g705g1161g18359g727g3 g252g252g3g20g19g19g705g1161g18359g727g3 g252g252g3g19g17g20 g3 g80g82g79g18g47g7696g8412g18344g19152g1161g18359g727g3 g252g252g3g27 g3 g80g80g82g79g18g47g3g49g68g50g43g452g3 g24g17g21g17g21g3 g51g38g53g169

22、07g2168g11528g3 g4018g1083g726g3 g252g252g3g55g68g84g3g39g49g36g13962g2616g18342g727g3 g252g252g3g71g49g55g51g86g9255g2616g10393g726g2357g6428g71g36g55g51g451g71g55g55g51g451g71g38g55g51g451g71g42g55g51g727g3 g252g252g3g20g19g1597g51g38g53g13635g2018g9186g708g2651g48g74 g21g14 g709g452g3 g8990g966g3

23、g16901g2162g11522g2357g2651g1206g51g38g53g6297g3790g6256g5621g19760g11444g2612g12285g18342g451g71g49g55g51g86g2554g48g74 g21g14 g12665g1944g18200g16901g2162g452g3 g24g17g21g17g22g3 g11115g9001g16907g2168g3 g4018g1083g726g3 g252g252g3g20g705g10860g14130g13062g727g3 g252g252g3g20g19g3g74g18g80g47g7784

24、g18344g7683g7113g727g3 g252g252g3g2256g7783g13635g2018g9186g727g3 g252g252g3g20g1597g55g36g40g11109g8995g13635g2018g9186g452g3 g24g17g21g17g23g3 g1964g4537g16907g2168g3 g4018g1083g726g3 g252g252g3g9885g14844g2556g15080g8804g727g3 g252g252g3g12071g18344g11520g13635g2018g9186g708g51g37g54g3g83g43g26g1

25、7g21g12g452g3 g3 g3 g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3 g20g12g3g55g85g76g93g82g79g928g3g39g49g36g6381g6656g16901g2162g708g2934g2801g2621g709g7263g11105g44g81g89g76g87g85g82g74g72g81g1948g2600g708g1483g5316g2934g7

26、09g6656g1483g11444g1239g2801g11444g2934g2801g2621g452g13577g2090g17929g1072g1553g5791g7263g1130g1206g7145g1519g7516g7735g2038g11444g1455g11096g13877g712 g5286g1085g16024g12138g4649g16917g1239g2801g11444g16852g2591g452g4018g7628g1958g4531g12665g7032g1239g2801g1959g7481g11560g2620g11444g7032g7628g712g

27、2121g2591g1455g11096g17929g1227g12665g7032g1239g2801g452g3 g39g37g23g24g18g55g3g21g22g20g20g252g21g19g21g20g3 g22g3 g24g17g21g17g24g3 g16907g2168g18307g13732g7151g8971g3 g16369g19572g5509g37g452g3 g24g17g22g3 g1312g3230g16884g3901g2754g1078g8535g5725g13901g7558g3 g24g17g22g17g20g3 g1312g3230g16884g3

28、901g3 g4018g1083g726g3 g252g252g3g51g38g53g1306g727g3 g252g252g3g11109g8995g1306g727g3 g252g252g3g13147g3910g7920g9083g1306g727g3 g252g252g3g2061g14118g6208g1791g13099g13583g727g3 g252g252g3g4671g3515g20744g17999g12267g5619g7530g727g3 g252g252g3g7163g9169g6495g14737g3224g727g3 g252g252g3g11844g19173

29、g6214g10731g10931g11844g12056g3224g727g3 g252g252g3g5598g18431g2256g7783g3224g727g3 g252g252g3g17333g2032g5141g1420g2592g452g3 g24g17g22g17g21g3 g1078g8535g5725g13901g7558g3 g4018g1083g726g3 g252g252g3g6267g3975g727g3 g252g252g3g12267g5619g12753g727g3 g252g252g3g51g38g53g2557g5316g12753g727g3 g252g2

30、52g3g2664g3940g452g3 g25g3 g6915g1426g7151g8971g3 g25g17g20g3 g7789g2807g2044g3901g3 g25g17g20g17g20g3 g7789g2807g18429g19708g2754g20154g3898g10812g3 g25g17g20g17g20g17g20g3 g2585g21873g2108g8962g10399g3 g11096g7929g6429g4480g2566g5557g7920g10379g11444g2579g21867g2102g8956g10393g712g13567g7184g14844

31、g51g37g54g9031g14177g712g25g24g19 g3 g74g12267g5619g20g19 g3 g80g76g81g15g2566g1082g9269g21g24g19 g3 g173g47g1483g5739g39g49g36g6381g6656g452g3 g25g17g20g17g20g17g21g3 g15990g9192g3 g5224g16372g18423g19702g5557g7920g10379g15984g9186g19g17g24 g3 g80g47g794g20 g3 g80g47g712g12539g2467g6457g27g29g20g87

32、08g1467g2256g1941g23g705g7696g8412g18344g1081g19152g6343g2061g2162g712g9255g2616g3447g2352g712g25g24g19 g3 g74g12267 g5619g24 g3 g80g76g81g712g19604g2539g15984g9078g15g11096g51g37g54g3416g11560g2620g12267g5619g2251g1083g712g12267g5619g9031g9172g15984g13558g14094g22g8529g712g19604g2539g1082g9269g9186

33、g712g11096g21g24g19 g3 g173g47g7184g14844g51g37g54g18429g7136 g5852g9118g15984g13558g14094g712g1483g5739g39g49g36g6381g6656g452g3 g25g17g20g17g20g17g22g3 g11259g7119g13562g13565g3 g2566g13556g13559g7783g2801g4018g4671g14032g451g14190g14143g451g14032g13099g14284g9211g5156g13571g12665g20 g3 g74g712g13

34、726g1214g11844g19173g6214g10731g10931g11844g12056g3224g1117g712g2256g1941g23 g3 g80g47g7184g14844g51g37g54g1909g2102 g11844g12056g712g7014g19702g13556g13559g5852g9186g712g2557g3901g2027g15805g22g8529g452g25g24g19 g3 g74g12267g5619g24 g3 g80g76g81g712g2566g1082g9269g9186g21g24g19 g3 g173g47g1483g5739

35、g39g49g36g6381g6656g452g3 g25g17g20g17g21g3 g11259g8712g2108g12273g3631g1969g10399g7020g14829g2560g3898g10812g3 g7014g14823g11201g5967g7683g44g37g53g57g451g1239g11087g11253g2568g11444g2437g4722g13558g14094g3625g1963g10393g712g2557g3901g2027g15805g22g8529g452g1301g25g24g19 g3 g74g12267g5619g24 g3 g80

36、g76g81g712g7014g19702g1082g9269g9186g712 g39g37g23g24g18g55g3g21g22g20g20g252g21g19g21g20g3 g23g3 g1483g5739g39g49g36g6381g6656g452g3 g25g17g21g3 g5563g7926g7789g2807g5745g39g49g36g6387g6662g3 g3416g13567g20148g3892g10806g11444g21g24g19 g3 g173g47g5557g7920g7783g2801g1117g2256g1941g55g85g76g93g82g79

37、g39g49g36g6381g6656g16901g2162g26g24g19 g3 g173g47g712g9255g2616g3447g2352g712g4564g9305g1420g11096g20g19 g3 g80g76g81g712 g13591g13597g2256g1941g21g19g19 g3 g173g47g8799g1327g712g6495g14737g9255g2352g712g4564g9305g7022g13726g20g24 g3 g80g76g81g712g1214g23 g3 g263g1083g26 g3 g21g19g19 g3 g74g12267g5

38、619g20g19 g3 g80g76g81g712g5427g1082g4722g9186g1411g712g7014 g19702g1117g19492g4722g2748g8799g1327g4722g9186g1411g712g2256g1941g20g19g19g705g1161g18359g22g19g19 g3 g173g47g712g20176g1602g9255g2616g3447g2352g712g4564g9305g7022g13726g20g19 g3 g80g76g81g712g1214g23 g3 g263g1083g24g3g19g19g19 g3 g74 g12

39、267g5619g20g19 g3 g80g76g81g712g5427g1082g9269g9186g712g2256g1941g19g17g20 g3 g80g82g79g18g47g3g7696g8412g18344g19152g1161g18359g20 g3 g80g47g712g4564g9305g7022g13726g22g19 g3 g80g76g81g712g1214g23 g3 g263g1083g24g3g19g19g19 g3 g74g12267g5619 g24 g3 g80g76g81g712g1981g5427g1082g9269g9186g712g8596g85

40、97g20692g18429g3901g22g8529g15g2256g1941g26g24g705g1161g18359g21 g3 g80g47g15g4564g9305g1420g11096g20g19 g3 g80g76g81g15g3g1214g23 g3 g263g1083g24g3g19g19g19 g3 g74g12267g5619g24 g3 g80g76g81g712 g5427g1082g9269g9186g15g4662g12267g5619g12753g1602g13726g1214g17333g2032g5141g1420g2592g1973g20222g5282g

41、20g19 g3 g80g76g81g15g7472g2622g2256g1941g27 g3 g80g80g82g79g18g47g3g49g68g50g43g18429g5852g5739g39g49g36 g3 g20g19g19 g3 g173g47g15g6381g6656 g4013g11444g39g49g36g13726g1214g16g21g19 g3 g263g1549g4488g452g3 g25g17g22g3 g49g72g86g87g72g71g3g51g38g53g7926g9089g3 g25g17g22g17g20g3 g7926g9089g6915g1426

42、g3 g25g17g22g17g20g17g20g3 g3916g3981g51g38g53g6915g1426g3 g3416g2016g9124g7569g1318g1083g712g6457g4018g1083g11096g18431g2102g2139g2256g1941g2612g16901g2162g726g3 g252g252g3g20g19g1597g51g38g53g13635g2018g9186g708g2651g48g74 g21g14 g709g712g21g17g24 g3 g173g47g727g3 g252g252g3g24 g3 g56g18g173g47g3g

43、55g68g84g3g39g49g36g13962g2616g18342g712g19g17g24 g3 g173g47g727g3 g252g252g3g44g37g53g57g3910g3975g5445g10393g41g20g2748g41g21g11g2612g24g19 g3 g83g80g82g79g18g47g709g712g2612g20 g3 g173g47g727g3 g252g252g3g39g49g36g712g21 g3 g173g47g727g3 g252g252g3g9885g14844g2556g15080g8804g712g16021g17379g14371

44、g5739g1411g12319g1130g21g24 g3 g173g47g452g3 g25g17g22g17g20g17g21g3 g3916g3981g51g38g53g2563g5322g12353g5317g3 g9259g2256g16901g2162g2622g12267g5619g9255g2616g712g4662g51g38g53g2557g5316g12753g13726g1214g51g38g53g1306g1973g712g6457g1301g1083g12347g5311g17931g15996g2557g5316g726g3 g68g12 g28g23 g3 g

45、263g20148g2568g5719g24 g3 g80g76g81g727g3 g69g12 g28g23 g3 g263g2568g5719g22g19 g3 g86g727g3 g70g12 g24g24 g3 g263g17968g9883g22g19 g3 g86g727g3 g71g12 g26g21 g3 g263g5414g1384g22g19 g3 g86g11444g5594g10719g712g1953g5594g10719g22g24g8529g712g7472g2622g1072g8529g26g21 g3 g263g5414g1384g20g19 g3 g80g7

46、6g81g712g13571g7567g6297g3790g727g3 g68g12 g51g38g53g1239g10393g11109g8995g6214g13726g1214g23 g3 g263g1549g4488g452g3 g25g17g22g17g20g17g22g3 g1979g3981g51g38g53g6915g1426g3 g3946g3910g3975g51g38g53g2557g5316g13571g7567g2622g712g3416g2016g9124g7569g1318g1083g712g6457g4018g1083g11096g18431g2102g2139g

47、2256g1941g2612g16901g2162g726g3 g252g252g3g20g19g1597g51g38g53g13635g2018g9186g708g2651g48g74 g21g14 g709g712g21g17g24 g3 g47g727g3 g252g252g3g24 g3 g56g18g47 g3 g55g68g84g3g39g49g36g13962g2616g18342g712g19g17g24 g3 g47g727g3 g252g252g3g44g37g53g57g1973g3975g5445g10393g41g22g2748g41g23g11g2612g24g19

48、 g3 g83g80g82g79g18g47g709g712g2612g20 g3 g47g727g3 g252g252g3g3910g3975g51g38g53g1239g10393g712g21 g3 g47g727g3 g252g252g3g9885g14844g2556g15080g8804g712g16021g17379g14371g5739g1411g12319g1130g21g24 g3 g47g452g3 g25g17g22g17g20g17g23g3 g1979g3981g51g38g53g2563g5322g12353g5317g3 g9259g2256g16901g216

49、2g2622g12267g5619g9255g2616g712g4662g51g38g53g2557g5316g12753g13726g1214g51g38g53g1306g1973g712g6457g1301g1083g12347g5311g17931g15996g2557g5316g726g3 g68g12 g28g23 g3 g263g20148g2568g5719g24 g3 g80g76g81g727g3 g69g12 g28g23 g3 g263g2568g5719g22g19 g3 g86g727g3 g70g12 g24g24 g3 g263g17968g9883g22g19 g3 g86g727g3 g39g37g23g24g18g55g3g21g22g20g20g252g21g19g21g20g3 g24g3 g71g12 g26g21 g3 g263g5414g1384g22g19 g3 g86g11444g5594g10719g712g1953g5594g10719g22g24g8529g712g7472g2622g1072g8529g26g21 g3 g263g5414g1384g20g19 g3 g80g76g81g712g13571g7567g6297g3790g727g3 g72g12 g51g38g53g1239g10393g11109g8995