Chapter 10- Classification of Microorganisms.ppt

1、Chapter 10: Classification of Microorganisms,Phylogeny: The Study of Evolutionary Relationships of Living Organisms Over 1.5 million different organisms have been identified to date.Many similarities among living organisms:Made up of cells surrounded by a plasma membrane.Use ATP as energy source.Sto

2、re genetic information as DNA.Ribosomes are the site of protein synthesis.Both differences and similarities among organisms are caused by natural selection (Darwin, 1858).Organisms can be classified into taxonomic categories (taxa), based on the differences and similarities among them.,Phylogeny: Th

3、e Study of Evolutionary Relationships of Living Organisms Ancient Greeks classified all living organisms into two groupsKingdom PlantaeKingdom Animalia In 1850s bacteria and fungi were incorrectly placed in the Plant Kingdom. In 1860s Kingdom Protista was proposed to include bacteria, fungi, algae,

4、and protozoa, but many scientists still classified bacteria and fungi as plants. Intense disagreement over classification of bacteria and fungi persisted over 100 years.,Phylogeny: The Study of Evolutionary Relationships of Living Organisms In 1930s electron microscopy made it clear that bacterial c

5、ells lacked a nucleus. The term procaryote was introduced in 1937.In 1959 Kingdom Fungi was established.In 1961 the current definition of the term procaryote was established.In 1968 the Kingdom Procaryotae was accepted by biologists.In 1969 Robert Whitaker proposed a five-kingdom system of biologica

6、l classification for all living organisms.,Five-Kingdom System of Biological ClassificationProposed in 1969 by Robert Whitaker : 1. Kingdom Procaryotae (Monera): Oldest known cells. Lived over 3.5 billion years ago. Lack a nucleus and membrane bound organelles. The other four kingdoms are eucaryotes

7、. Have a true nucleus and membrane bound organelles. 2. Kingdom Protista: Mostly unicellular, lack tissue organization. Most have flagella during life. 3. Kingdom Fungi: May be unicellular (yeasts) or multicellular (molds). Many are saprotrophs. 4. Kingdom Plantae: Multicellular, photosynthetic. 5.

8、Kingdom Animalia: Multicellular, heterotrophs that ingest food through a mouth or oral cavity.,Five-Kingdom Classification System,Differences Between Eucaryotic and Procaryotic CellsProcaryotes Eucaryotes Cell size 0.2-2 um in diameter 10-100 um in diameter Nucleus Absent Present Membranous Organell

9、es Absent Present Cell Wall Chemically complex When present, simple Ribosomes Smaller (70S) Larger (80S) in cell70S in organelles DNA Single circular Multiple linearchromosome chromosomes (histones) Cell Division Binary fission Mitosis,Procaryotes: Lack Nucleus and Membrane-Bound Organelles,Phylogen

10、y The Three Domain SystemDomain: In 1978 Carl Woese proposed this level of classification above kingdom.There are three domains based on the following distinguishing criteria: Cell wall composition Membrane lipids RNA sequence Protein synthesis Antibiotic sensitivity I. Domain Eubacteria: “True bact

11、eria”. II. Domain Archaeabacteria: “Ancient bacteria” III. Domain Eucarya: All eucaryotes: Protista, Fungi, Plantae, and Animalia.,Phylogeny: The Three Domain SystemRecent developments in molecular biology and biochemistry have revealed that there are two types of procaryotic cells, based on differe

12、nces in their ribosomes, cell walls, and metabolism. 1. Eubacteria: “True bacteria”. Cell wall contains peptidoglycan. Sensitive to antibiotics. 2. Archaeabacteria: “Ancient bacteria”Cell walls lack peptidoglycan, resistant to antibiotics. Live in extreme environmentsThree kingdoms: 1. Methanogens:

13、Strict anaerobes that produce methane. 2. Extreme Halophiles: Require high salt concentrations. 3. Thermoacidophiles: Live in hot, acidic environments.,Phylogenetic Relationships of Procaryotes,Classification of Organisms Scientific Nomenclature Scientific nomenclature: Universal system for naming a

14、nd classifying living organisms. Initially developed in the 18th century by Carl Linnaeus.Binomial nomenclature: Each organism (species) has a two part name. Names are either italicized or underlined.Genus name: Always capitalized, always a noun. May use initial.species name: Always lower case, usua

15、lly an adjective.Names are usually derived from Latin (or Greek) or may have latinized endings. Examples: Homo sapiens (H. sapiens): HumanPenicillium notatum (P. notatum): Mold that produces penicillinCanis familiaris (C. familiaris): Domestic dog,Classification of Organisms Hierarchy of Taxonomic C

16、ategories DOMAIN Kingdom Phylum or Division (Bacteria)ClassOrderFamilyGenusspecies,Taxonomic Categories,Division (Bacteria),Classification of Bacteria Scientific Nomenclature Bacterial species: Population of cells with similar characteristics.Bacterial strain: A subgroup of a bacterial species that

17、has distinguishing characteristics. Identified by numbers, letters, or names that follow the scientific name.Escherichia coli O157:H7: Strain that causes bloody diarrhea.Bergeys Manual: Provides a reference for identifying and classifying bacteria.Classification initially based on cell morphology, s

18、taining, metabolism, biochemistry, serology, etc.More recently, DNA, RNA, and protein sequence analysis are being used to study evolutionary relationships.,Classification of VirusesViruses are not considered living organisms by most biologists, because they lack cells and their own anabolic machinery. Obligate intracellular parasites. Must have evolved after their host cell evolved.Viral species: Population of viruses with similar characteristics that occupies a particular ecological niche.MorphologyGenesEnzymes,