BS ISO 856-2006 en_7750 Oil of peppermint (Mentha x piperita L.)《薄荷油(Mentha x piperita L.)》.pdf

1、BRITISH STANDARD BS ISO 856:2006 Oil of peppermint (Mentha piperita L.)ICS 71.100.60 BS ISO 856:2006 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 May 2006 BSI 2006 ISBN 0 580 48334 7 National foreword This British Standard reproduces ve

2、rbatim ISO 856:2006 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils, which has the responsibility to: A list of organizations represented on this committee can be obtained on request to its secretary. C

3、ross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue or of British S

4、tandards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to understand the text; present to

5、the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document comprises a front cover, an inside fron

6、t cover, the ISO title page, pages ii to iv, pages 1 to 12, an inside back cover and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. Date Comments Reference number ISO 856:2006(E)INTERNATIONAL

7、 STANDARD ISO 856 Second edition 2006-04-15 Oil of peppermint (Mentha piperita L.) Huile essentielle de menthe poivre (Mentha piperita L.) BS ISO 856:2006ii iii Contents Page Foreword iv 1 Scope . 1 2 Normative references . 1 3 Terms and definitions. 1 4 Requirements 1 4.1 Appearance . 1 4.2 Colour

8、1 4.3 Odour . 1 4.4 Relative density at 20 C, d 20 20 . 1 4.5 Refractive index at 20 C 1 4.6 Optical rotation at 20 C . 2 4.7 Miscibility in ethanol, 70 % (volume fraction), at 20 C 2 4.8 Acid value 2 4.9 Chromatographic profile 2 4.10 Flashpoint 2 5 Sampling 3 6 Test methods. 3 6.1 Relative density

9、 at 20 C, d 20 20 . 3 6.2 Refractive index at 20 C 3 6.3 Optical rotation at 20 C . 3 6.4 Miscibility in ethanol, 70 % (volume fraction), at 20 C 3 6.5 Acid value 3 6.6 Chromatographic profile 3 7 Packaging, labelling, marking and storage 3 Annex A (informative) Typical chromatograms of the analysis

10、 by gas chromatography of the oil of peppermint (Mentha piperita L.). 4 Annex B (informative) Flashpoint . 11 Bibliography . 12 BS ISO 856:2006iv Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of

11、preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental,

12、 in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main t

13、ask of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attent

14、ion is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 856 was prepared by Technical Committee ISO/TC 54, Essential oils. This second edition cancels and repl

15、aces the first edition (ISO 856:1981), which has been technically revised. BS ISO 856:20061 Oil of peppermint (Mentha piperita L.) 1 Scope This International Standard specifies certain characteristics of the oil of peppermint (Mentha piperita L.), with a view to facilitate assessment of its quality.

16、 NOTE As it is difficult to differentiate the specification of peppermint oils (Mentha piperita L.) from different origins, they have been regrouped in this International Standard. The following origins have been taken into consideration: USA, United Kingdom, France, Italy, India and China. 2 Normat

17、ive references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General

18、 rules for packaging, conditioning and storage ISO/TR 211, Essential oils General rules for labelling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive inde

19、x ISO 592, Essential oils Determination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 1242, Essential oils Determination of acid value ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for p

20、resentation in standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 oil of peppermint e

21、ssential oil obtained by steam distillation of the aerial parts of the plant Mentha piperita L., of the Lamiaceae family NOTE For information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1 Appearance Clear, mobile liquid. 4.2 Colour Almost colourless to pale greenish yellow. 4.3 Odour Chara

22、cteristic of mint, sweet and menthol-like, with some variations depending on the origin. 4.4 Relative density at 20 C, d 20 20Origins other than US US type Min. 0,898 0,899 Max. 0,918 0,911 4.5 Refractive index at 20 C Minimum: 1,459 0 Maximum: 1,465 0 BS ISO 856:20062 4.6 Optical rotation at 20 C O

23、rigins other than US US type 30 to 14 32 to 18 4.7 Miscibility in ethanol, 70 % (volume fraction), at 20 C For origins other than US, it shall not be necessary to use more than 5 volumes of ethanol, 70 % (volume fraction), to obtain a clear solution with 1 volume of essential oil. For the US type, i

24、t shall not be necessary to use more than 3 volumes of ethanol, 70 % (volume fraction), to obtain a clear solution with 1 volume of essential oil. 4.8 Acid value Maximum: 2 4.9 Chromatographic profile Analysis of the essential oil shall be carried out by gas chromatography. In the chromatogram obtai

25、ned, the representative and characteristics components shown in Table 1 shall be identified. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil. 4.10 Flashpoint Information on the flashpoin

26、t is given in Annex B. Table 1 Chromatographic profile Origins other than US US type Components Min. (%) Max. (%) Min. (%) Max. (%) 3-Octanol 0,1 0,5 0,1 0,4 1,8-Cineole 3,0 8,0 4,0 6,0 Limonene a1,0 3,0 1,0 2,5 trans-Sabinene hydrate 0,5 2,0 0,5 2,3 Menthone 13,0 28,0 15,0 25,0 Isomenthone 2,0 8,0

27、2,0 4,5 Menthofuran 1,0 8,0 1,5 6,0 Neomenthol 2,0 6,0 2,5 4,5 Menthol 32,0 49,0 36,0 46,0 Pulegone 0,5 3 0,5 2,5 Menthyl acetate b2,0 8,0 3,0 6,5 -Caryophyllene 1,0 3,5 1,0 2,5 NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A. aThe li

28、monene is regarded to be predominantly L-limonene based on the physical tests. It is believed that there might be a small amount of D-limonene present but the exact quantity is unknown. bThe menthyl acetate is regarded to be predominantly L-menthyl acetate based on the physical tests. It is believed

29、 that there might be a small amount of D-menthyl acetate present but the exact quantity is unknown. BS ISO 856:20063 5 Sampling See ISO 212. Minimum volume of final sample: 25 ml. NOTE This volume allows each of the tests specified in this International Standard to be carried out at least once. 6 Te

30、st methods 6.1 Relative density at 20 C, d 20 20See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Miscibility in ethanol, 70 % (volume fraction), at 20 C See ISO 875. 6.5 Acid value See ISO 1242. 6.6 Chromatographic profile See ISO 11024-1 and ISO 1

31、1024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BS ISO 856:20064 Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the oil of peppermint (Mentha piperita L.) BS ISO 856:20065 A.1 Typical chromatograms of the analysis of the oil of

32、 peppermint, France (Provence) Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 12 3-Octanol + myrcene 1,8-Cineole Limonene trans-Sabinene hydrate Menthone Isomenthone Menthofuran Neomenthol Menthol Pulegone Menthyl acetate -Caryophyllene Column: silica capillary; length 50 m; intern

33、al diameter 0,2 mm Stationary phase: poly(dimethyl siloxane) Film thickness: 0,25 m Oven temperature: temperature programming from 65 C to 230 C at a rate of 2 C/min Injector temperature: 230 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l C

34、arrier gas flow rate: 1,1 ml/min Split ratio: 1/100 Figure A.1 Typical chromatogram taken on an apolar column BS ISO 856:20066 Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 12 Limonene 1,8-Cineole 3-Octanol Menthone trans-Sabinene hydrate Menthofuran Isomenthone Menthyl acetate -C

35、aryophyllene Neomenthol + terpinen-4-ol Pulegone Menthol Column: silica capillary; length 50 m; internal diameter 0,2 mm Stationary phase: poly(ethylene glycol) (Carbowax 20 M) Film thickness: 0,25 m Oven temperature: temperature programming from 65 C to 230 C at a rate of 2 C/min Injector temperatu

36、re: 230 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l Carrier gas flow rate: 1,1 ml/min Split ratio: 1/100 Figure A.2 Typical chromatogram taken on a polar column BS ISO 856:20067 A.2 Typical chromatograms of the analysis of the oil of pep

37、permint, India Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 12 13 3-Octanol Myrcene 1,8-Cineole Limonene trans-Sabinene hydrate Menthone Isomenthone Menthofuran Neomenthol Menthol Pulegone Menthyl acetate -Caryophyllene Column: silica capillary; length 50 m; internal diameter 0,2

38、 mm Stationary phase: poly(dimethyl siloxane) Film thickness: 0,25 m Oven temperature: temperature programming from 65 C to 230 C at a rate of 2 C/min Injector temperature: 230 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l Carrier gas flow

39、 rate: 1,1 ml/min Split ratio: 1/100 Figure A.3 Typical chromatogram taken on an apolar column BS ISO 856:20068 Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Myrcene -Terpinene Limonene 1,8-Cineole 3-Octanol trans-Sabinene hydrate Menthone Menthofuran Isomenthone Menth

40、yl acetate Isoneo-isopulegol Neomenthol -Caryophyllene Menthol Pulegone Column: silica capillary; length 60 m; internal diameter 0,2 mm Stationary phase: poly(ethylene glycol) (Supelcowax 10) Film thickness: 0,20 m Oven temperature: temperature programming from 80 C to 180 C at a rate of 1 C/min Inj

41、ector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate: 30 ml/min Split ratio: 1/100 Figure A.4 Typical chromatogram taken on a polar column BS ISO 856:20069 A.3 Typical chromatogram of the analysis of the

42、 oil of peppermint, USA (Idaho) Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Myrcene -Terpinene Limonene 1,8-Cineole 3-Octanol trans-Sabinene hydrate Menthone Menthofuran Isomenthone Menthyl acetate Isoneo-isopulegol Neomenthol -Caryophyllene Menthol Pulegone Column:

43、silica capillary; length 60 m; internal diameter 0,2 mm Stationary phase: poly(ethylene glycol) (Supelcowax 10) Film thickness: 0,20 m Oven temperature: temperature programming from 80 C to 180 C at a rate of 1 C/min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization

44、type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate: 30 ml/min Split ratio: 1/100 Figure A.5 Typical chromatogram taken on a polar column BS ISO 856:200610 A.4 Typical chromatogram of the analysis of the oil of peppermint, USA (Willamette) Peak identification Operating conditions 1

45、 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Myrcene -Terpinene Limonene 1,8-Cineole 3-Octanol trans-Sabinene hydrate Menthone Menthofuran Isomenthone Menthyl acetate Isoneo-isopulegol Neomenthol -Caryophyllene Menthol Pulegone Column: silica capillary; length 60 m; internal diameter 0,2 mm Stationary phase:

46、poly(ethylene glycol) (Supelcowax 10) Film thickness: 0,20 m Oven temperature: temperature programming from 80 C to 180 C at a rate of 1 C/min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate: 30

47、ml/min Split ratio: 1/100 Figure A.6 Typical chromatogram taken on a polar column BS ISO 856:200611 Annex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies and people in charge of safety services require information on the flashpoints of

48、essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that: there is wide variation in the chemical composition of esse

49、ntial oils; the volume of the sample needed for certain requirements would be too costly for high-priced essential oils; as there are several different types of equipment which can be used for the determination, users cannot be expected to use one specified type only. Consequently, it was decided to give a mean value for the flashpoint in an informat