1、BSI Standards Publication BS ISO 9345-2:2014 Microscopes Imaging distances related to mechanical reference planes Part 2: Infinity-corrected optical systemsBS ISO 9345-2:2014 BRITISH STANDARD National foreword This British Standard is the UK implementation of ISO 9345-2:2014. It supersedes BS ISO 93
2、45-2:2003 which is withdrawn. The UK participation in its preparation was entrusted to Technical Committee CPW/172, Optics and Photonics. A list of organizations represented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary
3、provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2014. Published by BSI Standards Limited 2014 ISBN 978 0 580 84506 2 ICS 37.020 Compliance with a British Standard cannot confer immunity from legal obligations. This British Standard was p
4、ublished under the authority of the Standards Policy and Strategy Committee on 30 September 2014. Amendments issued since publication Date Text affectedBS ISO 9345-2:2014 ISO 2014 Microscopes Imaging distances related to mechanical reference planes Part 2: Infinity-corrected optical systems Microsco
5、pes Tirages mcaniques en fonction des plans mcaniques de rfrence Partie 2: Systmes doptique corrigs linfini INTERNATIONAL STANDARD ISO 9345-2 Second edition 2014-09-15 Reference number ISO 9345-2:2014(E)BS ISO 9345-2:2014ISO 9345-2:2014(E)ii ISO 2014 All rights reserved COPYRIGHT PROTECTED DOCUMENT
6、ISO 2014 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be
7、requested from either ISO at the address below or ISOs member body in the country of the requester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org Published in SwitzerlandBS ISO 9345-2:2014ISO 9345-2:2014(E)
8、 ISO 2014 All rights reserved iii Contents Page Foreword iv 1 Scope . 1 2 Normative references 1 3 Terms and definitions . 1 4 Requirements 2 4.1 Nominal dimensions and tolerances 2 4.2 Examples 5 5 Marking 5 Annex A (informative) Examples of dimensions in use . 6 Annex B (informative) Requirements
9、and recommendations for OEM-use of objectives and tube lenses 7 Bibliography 9BS ISO 9345-2:2014ISO 9345-2:2014(E) Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standar
10、ds is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take
11、 part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In par
12、ticular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives). Attention is drawn to the possibility that some of the elements of
13、 this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see
14、www.iso.org/patents). Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement. For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence t
15、o the WTO principles in the Technical Barriers to Trade (TBT) see the following URL: Foreword - Supplementary information The committee responsible for this document is ISO/TC 172, Optics and photonics, Subcommittee SC 5, Microscopes and endoscopes. This second edition cancels and replaces the first
16、 edition (ISO 9345-2:2003), which has been technically revised. ISO 9345 consists of the following parts, under the general title Microscopes Imaging distances related to mechanical reference planes: Part 1: Tube length 160 mm Part 2 Infinity-corrected optical systemsiv ISO 2014 All rights reservedB
17、S ISO 9345-2:2014INTERNATIONAL ST ANDARD ISO 9345-2:2014(E) Microscopes Imaging distances related to mechanical reference planes Part 2: Infinity-corrected optical systems 1 Scope This part of ISO 9345 specifies the imaging distances of objectives, eyepieces and the focal length of “normal” tube len
18、ses of microscopes with infinity-corrected optical systems. NOTE A specific combination of eyepiece, objective, and tube lens is frequently used to correct aberrations. Therefore the combination of an objective from one manufacturer and the tube lens or eyepiece from another manufacturer, although c
19、onforming to this part of ISO 9345, can cause errors in magnification and/or in optical performance. 2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cite
20、d applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 9345-1, Microscopes Imaging distances related to mechanical reference planes Part 1: Tube length 160 mm 3 Terms and definitions For the purposes of this document, the following te
21、rms and definitions apply. 3.1 parfocalizing distance of the objective l 1 distance in air between the object plane (i.e. the uncovered surface of the object) and the locating flange of the objective, when the microscope is in its working position Note 1 to entry: The parfocalizing distance of the o
22、bjective is one of the optical interfacing dimensions. SOURCE: ISO 10934-1:2002, 2.80.2.4 Note 2 to entry: See Figure 1, Figure 2 and footnote b in Table 1. 3.2 image distance of the objective l 2 distance in air between the objective locating surface and the primary image plane Note 1 to entry: An
23、infinity-corrected objective alone produces a primary image at infinity. In combination with an infinity-corrected tube lens, the primary image is produced in the back focal plane of this tube lens (see Figure 1). ISO 2014 All rights reserved 1BS ISO 9345-2:2014ISO 9345-2:2014(E) 3.3 parfocalizing d
24、istance of the eyepiece l 3 distance between the locating flange of the eyepiece and the plane upon which the eyepiece is focused Note 1 to entry: The plane upon which the eyepiece is focused is coincident with the plane of the final real image of the microscope when the eyepiece is mounted in the v
25、iewing tube. The parfocalizing distance of the eyepiece is one of the optical interfacing dimensions, and is commonly 10 mm. SOURCE: ISO 10934-1:2002, 2.80.2.3 Note 2 to entry: This plane is coincident with the primary image plane of the microscope when the eyepiece is mounted in the viewing tube (s
26、ee Figure 1). 3.4 focal length of the “normal” tube lens f NTL focal length related to the magnification and the focal length of the objectives which are designed to operate with this tube lens 4 Requirements 4.1 Nominal dimensions and tolerances The nominal dimensions shall be as given in Table 1 a
27、nd as illustrated in Figure 1.2 ISO 2014 All rights reservedBS ISO 9345-2:2014ISO 9345-2:2014(E) Table 1 Nominal dimensions and tolerances Feature Symbol Nominal values/range mm Numerical aperture Tolerance mm Parfocalizing distance of objective a,b l 1 45 + 15 k (k = 1, 0, 1, 2, 3, 4) 0,1 0,2 c 0,1
28、 to 0,25 0,06 0,25 to 0,45 0,03 0,45 0,01 Image distance of objective d l 2 Parfocalizing distance of eyepiece l 3 10 0,2 Focal length of “normal” tube lens e f NTL 150 f NTL 250 aThe choice of a parfocalizing distance for an objective depends on the design concept of the microscope as a whole. The
29、parfocalizing distance, l 1= 45 mm of objectives, has become the standard value for microscopes with tube length 160 mm (see ISO 9345-1) and has been adopted for various existing infinity-corrected microscope systems. Examples of common values in use are given in Annex A. bThe parfocalizing distance
30、, l 1 , shown in Figure 1 and Table 1, is intended to apply to objectives when used with uncovered objects (specimens). Objectives for use with objects covered by a cover glass shall have the following parfocalizing distance, to allow for the virtual displacement of the object by the cover glass (se
31、e also Figure 2): where:t is the thickness of cover glass;n is the refractive index of the cover glass. cThe tolerance 0,2 mm for the parfocalizing distance of objectives with numerical aperture 0,1 does not necessarily apply to objectives with magnifications lower than 4 . dIn infinity-corrected op
32、tical systems, the primary image is always produced by the objective in combination with a tube lens. The distance between the locating flange of the objective and the tube lens depends on the design of the microscope. The microscope shall have such a design that, in combination with objectives and
33、tube lenses in accordance with this part of ISO 9345, the primary image is produced 10 mm below the eyepiece-locating surface of the viewing tube. eThe choice of focal length for a “normal” tube lens depends on the design concept of the microscope system. Its value shall be in the range of 150 mm f
34、NTL 250 mm. Examples of common values in use are given in Annex A. ISO 2014 All rights reserved 3BS ISO 9345-2:2014ISO 9345-2:2014(E) Key 1 eyepiece-locating surface of the viewing tube 2 primary image plane 3 locating flange of the eyepiece 4 tube lens 5 locating flange of the objective 6 objective
35、-locating surface (of the nosepiece) 7 object plane Figure 1 Locating surfaces, reference planes, and imaging distances4 ISO 2014 All rights reservedBS ISO 9345-2:2014ISO 9345-2:2014(E) 4.2 Examples Figure 2 illustrates the influence of different cover glass thicknesses on the parfocalizing distance
36、. Dimensions in millimetres Uncovered object t = 0 mm Object with cover glass t = 0,17 mm thickness n = 1,5 Object with culture chamber t = 1,5 mm bottom thickness n = 1,5 l CG= l 1 a l CG= l 1+ 0,06 mm a l CG= l 1+ 0,5 mm a al CGis the resulting parfocalizing distance due to different cover glass t
37、hickness. Figure 2 Examples of parfocalizing distances as function of cover glass thickness 5 Marking If the magnification of the primary image is changed by built-in optical systems, the tube factor shall be marked on the magnification changing component (stand, tube, etc.); e.g. 1,25 . ISO 2014 Al
38、l rights reserved 5BS ISO 9345-2:2014ISO 9345-2:2014(E) Annex A (informative) Examples of dimensions in use Table A.1 shows the nominal values l 1 , l 2 , l 3,and f NTLused by the main microscope manufacturers (in alphabetical order) at the time of publication of this part of ISO 9345. Table A.1 Exa
39、mples of dimensions l 1 , l 2 , l 3and f NTLin use Dimensions in millimetres Feature Leica Nikon Olympus Zeiss Parfocalizing distance of objective, l 1 45 45/60 45 45 Image distance of objective, l 2 Parfocalizing distance of eyepiece, l 3 10 10 10 10 Focal length of “normal” tube lens, f NTL 200 20
40、0 180 1606 ISO 2014 All rights reservedBS ISO 9345-2:2014ISO 9345-2:2014(E) Annex B (informative) Requirements and recommendations for OEM-use of objectives and tube lenses B.1 General In case of adaptation of infinity-corrected objectives and tube lenses to instruments and equipment other than the
41、microscopes of the manufacturer, the user of these components needs additional dimensional information from the manufacturer for proper assembly. This annex contains recommendations for the minimum information that should be transferred to the OEM. NOTE A specific combination of eyepiece, objective,
42、 and tube lens is frequently used to correct aberrations. Therefore, the combination of an objective from one manufacturer and the tube lens or eyepiece from another manufacturer, although conforming to this part of ISO 9345, can cause errors in magnification and/or in optical performance. B.2 Terms
43、 and definitions B.2.1 image distance of the tube lens l NTL distance between the primary image plane and the locating flange of the tube lens Note 1 to entry: It depends on the optical and mechanical design concept and is an important dimen- sion for OEM use. B.2.2 distance between objective and tu
44、be lens d distance between the locating flange of the objective and the locating flange of the tube lens Note 1 to entry: It depends on the optical and mechanical design concept. To ensure correct optical performance for OEM the indication of the minimum and maximum value is recommended. ISO 2014 Al
45、l rights reserved 7BS ISO 9345-2:2014ISO 9345-2:2014(E) B.3 Recommended information for the user See Figure B.1. Key 1 locating flange of the objective 2 locating flange of the tube lens 3 primary image NOTE The external dimensions of the tube lens mount, the method of fixing (screw thread etc.) and
46、 the screw thread of the objectives (see ISO 8038) for which it is designed and the position of the locating flange should be indicated by the manufacturer. Figure B.1 Assembling dimensions for objectives and tube lenses8 ISO 2014 All rights reservedBS ISO 9345-2:2014ISO 9345-2:2014(E) Bibliography
47、1 ISO 8038, Microscopes Screw threads for objectives and related nosepieces 2 ISO 10934-1, Optics and optical instruments Vocabulary for microscopy Part 1: Light microscopy ISO 2014 All rights reserved 9BS ISO 9345-2:2014ISO 9345-2:2014(E) ISO 2014 All rights reserved ICS 37.020 Price based on 9 pag
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