1、Designation: E1092 91 (Reapproved 2010)Standard Specification forGlass Micro Folin Pipet, Disposable1This standard is issued under the fixed designation E1092; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year of last revisi
2、on. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 This specification covers a glass disposable micro Folinpipet suitable for use in micro techniques for estimation ofblood sugar b
3、y the Folin method.1.2 The values stated in SI units are to be regarded asstandard. No other units of measurement are included in thisstandard.1.3 The following precautionary statement pertains only tothe test method portion, Section 8, of this specification. Thisstandard does not purport to address
4、 all of the safety concerns,if any, associated with its use. It is the responsibility of the userof this standard to establish appropriate safety and healthpractices and determine the applicability of regulatory limita-tions prior to use.2. Referenced Documents2.1 ASTM Standards:2E438 Specification
5、for Glasses in Laboratory Apparatus2.2 ISO Standard:1769 Laboratory GlasswarePipettesColor Coding33. Terminology3.1 Definitions of Terms Specific to This Standard:3.1.1 accuracy, nthe expected distribution of mean vol-umes around the stated volume.3.1.2 coeffcient of variation, nthe expected distrib
6、utionof individual volumes around the mean volume.3.1.3 disposable, nmicro Folin pipets that are intended tobe used once only and then discarded.NOTE 1Such pipets will only be expected to provide their specifiedperformance during the original operation.4. Classification4.1 This specification covers
7、only one glass pipet as illus-trated in Fig. X1.1.5. Materials and Manufacture5.1 The pipet shall be made of borosilicate glass, Type 1,Class B, or soda lime glass, Type 2, in accordance withSpecification E438.6. Physical Properties6.1 DesignThe micro Folin pipet shall be made of onepiece constructi
8、on glass tubing that is straight and uniform borewith a point pulled on one end to the dimensions as specifiedin Fig. X1.1.6.2 DimensionsThe pipet shall be made of tubing with anoutside diameter (o.d.) of 2.5 6 0.1 mm with an insidediameter (i.d.) of 1.8 6 0.1 mm. The uniformity of the boreshall be
9、60.05 mm throughout the straight portion of the pipet.The pipet shall be 183 6 1 mm long with all dimensions andtolerances as shown in Fig. X1.1.6.3 CapacityThe pipet shall be calibrated “to contain”(T.C.) 0.1 and 0.2 mL at 20C. Marking shall be as specified in6.5.6.3.1 Accuracy (see 3.1.1)The accur
10、acy from stated vol-ume shall be 61.0 % for the 0.1 and 0.2-mL capacity and shallbe determined as specified in 8.1.6.3.2 Coeffcient of Variation (see 3.1.2)The coefficient ofvariation from stated volume for the 0.1 and 0.2-mL capacityshall not exceed 1.25 % and shall be determined as specified in8.1
11、.6.4 Graduation LinesThe pipet shall be calibrated andmarked with graduation lines at 0.1 and 0.2 mL from the tip ofthe pipet. The graduation lines shall be 0.3 6 0.1 mm and shallcompletely encircle the tube.1This specification is under the jurisdiction of ASTM Committee E41 onLaboratory Apparatus a
12、nd is the direct responsibility of Subcommittee E41.01 onApparatus.Current edition approved Sept. 1, 2010. Published September 2010. Originallyapproved in 1986. Last previous edition approved in 2005 as E1092 91 (2005).DOI: 10.1520/E1092-91R10.2For referenced ASTM standards, visit the ASTM website,
13、www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.3Available from International Organization for Standardization (ISO), 1, ch. dela Voie-Creuse, Case postale 56, CH-12
14、11, Geneva 20, Switzerland, http:/www.iso.ch.1Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.6.5 Pipet NomenclatureThe pipet shall be marked with0.1 and 0.2-mL markings slightly above the graduation lines.The pipet may be marked with
15、 the inscription TC 20C, or themanufacturers or vendors name or trademark, or both.6.6 Color CodingThe pipet shall be color coded forcapacity as specified in ISO Standard 1769 with an “orange”color band that is 6 6 2 mm wide and located 16 6 2 mm fromthe top of the pipet as shown in Fig. X1.1.6.7 Ma
16、rking PermanencyGraduation lines, inscriptions,and numerical markings on the pipet (other than the color codeband) shall be black in color. All markings may not be ofpermanent nature but must possess sufficient stability to endurenormal transportation and its expected one-time use and mustmeet the t
17、est requirements as specified in 8.5.6.8 Lot ControlA lot or control number shall be indicatedon the pipet container package. This lot or control number shallbe traceable to the origin and purchase order of raw materialglass tubing.7. Workmanship, Finish and Appearance7.1 The pipet shall be as free
18、as possible of visible defectsthat would detract from its appearance or impair its service-ability when viewed by the human eye under normal roomlighting.8. Test Method8.1 Capacity:8.1.1 Weighing Volume, Using WaterAllow a small vessel(5-mL beaker) and a container of distilled water to stand atroom
19、temperature of 20 to 25C for 2 h. Weigh the dry pipet,record the weight, and set aside. Weigh the dry vessel andrecord the weight. Fill the pipet with water and adjust to thecalibration line. Deliver the contents of the pipet in theprescribed manner into the vessel. Reweigh the vessel withwater cont
20、ent and record the weight.8.1.2 Immediately reweigh the wet pipet and record theweight. Record the room temperature. Subtract the recordedweight of the dry vessel from the recorded weight of the watercontaining vessel to obtain the apparent mass of the containedwater. Subtract the recorded weight of
21、 the dry pipet from thatof the wet pipet to obtain the apparent mass of the water stillcontained within the pipet. Add the apparent mass within thevessel to that within the pipet to get the total apparent masscontained within the filled pipet. Calculate the volume, v,inaccordance with the appendix.N
22、OTE 2To accurately perform this test method, the reliability of theweighing instrument used should be confirmed against a known standardand the weighing instrument should possess a minimum sensitivity of0.01 mg.8.2 CalculationCalculate the volume, v, of a micropipetfrom the weighings, in air, as fol
23、lows:v 5 W 3 Z(1)where:W = apparent mass of liquid (water), weighed in air, andZ = apparent specific volume of liquid (water).Values of Z for water are given in the appendix.8.3 Capacity Deviation (single pipet)In accordance withthe methods outlined, capacity deviation is the differencebetween the s
24、tated capacity and the observed capacity of thepipet as follows:Capacity deviations,%5 Vc2 V1! 3 100/V1(2)Vc5 Vt/1 1 at 2 20C! (3)where:Vt= observed volumetric capacity at tC, uL,Vc= corrected volumetric capacity at 20C, uL,a = coefficient of cubical expansion of pipet glass equals0.000010/C for Typ
25、e 1, Class A (borosilicate);0.000015/C for Type 1, Class B (noncorrosive boro-silicate); and 0.000025/C for Type 2 (soda-lime),V1= stated capacity of pipet, uL, andt = temperature, C.8.4 Capacity Deviation (number of pipets)Test a mini-mum of 30 pipets taken at random from a completed manu-factured
26、production lot. Calculate the volumetric deviation forthe 30 pipets as follows:8.4.1 Accuracy:Accuracy, % 5 100x 2 V1!/V1(4)where:x = mean of sample measurements, andV1= stated capacity of pipet.8.4.2 Coeffcient of Variation:Coefficient of variation, % 5 100s/xs5 =x 2 x!2/n 2 1(5)where:x = individua
27、l sample measurement,x = mean of sample measurements, andn = number of pipets measured.8.5 Pipet Marking Permanency TestImmerse the tube indistilled water for 30 s. Using a soft paper tissue, wipe themarked portion of the pipet lightly with ten complete strokes(five up and five down). When judged by
28、 the naked eye undernormal room lighting the pipet markings should appear asbefore the test with only possible lightening of the markings orminor removal of the markings, or both, that would not effectthe pipets serviceability.9. Keywords9.1 disposable; glass; micro Folin; pipetE1092 91 (2010)2APPEN
29、DIX(Nonmandatory Information)X1. See Table X1.1 and Fig. X1.1.ASTM International takes no position respecting the validity of any patent rights asserted in connection with any item mentionedin this standard. Users of this standard are expressly advised that determination of the validity of any such
30、patent rights, and the riskof infringement of such rights, are entirely their own responsibility.This standard is subject to revision at any time by the responsible technical committee and must be reviewed every five years andif not revised, either reapproved or withdrawn. Your comments are invited
31、either for revision of this standard or for additional standardsand should be addressed to ASTM International Headquarters. Your comments will receive careful consideration at a meeting of theresponsible technical committee, which you may attend. If you feel that your comments have not received a fa
32、ir hearing you shouldmake your views known to the ASTM Committee on Standards, at the address shown below.This standard is copyrighted by ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959,United States. Individual reprints (single or multiple copies) of this st
33、andard may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org). Permission rights to photocopy the standard may also be secured from the ASTM website (www.astm.org/COPYRIGHT/).TABLE
34、X1.1 Density and Z Factor for WaterTemperatureCDensity,g/cm3Z,cm3/g20 0.99820 1.002921 0.99799 1.003122 0.99777 1.003323 0.99754 1.003524 0.99729 1.003725 0.99704 1.004026 0.99678 1.004227 0.99651 1.004528 0.99623 1.004729 0.99594 1.005130 0.99564 1.0054FIG. X1.1 Glass Micro Folin Pipet, DisposableE1092 91 (2010)3
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