ASTM E1293-2002(2007) Standard Specification for Glass Measuring Pipets《玻璃测量移液管的标准规范》.pdf

1、Designation: E 1293 02 (Reapproved 2007)Standard Specification forGlass Measuring Pipets1This standard is issued under the fixed designation E 1293; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year of last revision. A numbe

2、r in parentheses indicates the year of last reapproval. Asuperscript epsilon (e) indicates an editorial change since the last revision or reapproval.1. Scope1.1 This specification covers requirements for glass measur-ing pipets of precision and general purpose grades, used inmeasuring volumes of liq

3、uids.2. Referenced Documents2.1 ASTM Standards:2E 438 Specification for Glasses in Laboratory ApparatusE 542 Practice for Calibration of Laboratory VolumetricApparatusE 671 Specification for Maximum Permissible Thermal Re-sidual Stress in Annealed Glass Laboratory ApparatusE 694 Specification for La

4、boratory Glass Volumetric Appa-ratusE 920 Specification for Commercially Packaged LaboratoryApparatusE 921 Specification for Export Packaged Laboratory Appa-ratusE 1133 Practice for Performance Testing of Packaged Labo-ratory Apparatus for United States Government Procure-mentsE 1157 Specification f

5、or Sampling and Testing of ReusableLaboratory GlasswareE 1273 Specification for Color Coding of Reusable Labo-ratory Pipets3. Classification3.1 Style, Class and CapacityPipets covered by thisspecification shall be of the following styles, classes, andcapacities:3.1.1 Style 1Standard taper tip.3.1.1.

6、1 Class AEach pipet of precision grade shall bemarked with the letter “A” to signify compliance with appli-cable construction and accuracy requirements in Table 1. Eachpipet may be marked with a permanent serial number at theoption of the manufacturer.3.1.1.2 Class BGeneral purpose pipets are of the

7、 samebasic design as Class A pipets. Volumetric tolerances for ClassB pipets shall be within twice the specified range allowed forClass A pipets. These pipets need not be marked with theirclass designation or serial number. See Table 1 for tolerancesand dimensional requirements.3.1.2 Style 2Long tap

8、er tip (Class B only).3.1.3 Capacities, (mL)0.1, 0.2, 0.5, 1, 2, 5, 10, 25, and 50.4. Design4.1 ShapeThe pipets shall be straight and of one-piececonstruction. Any cross section of a pipet taken in a planeperpendicular to the longitudinal axis shall be circular.4.2 Delivery TipsDelivery tips of Styl

9、e 1 pipets shall bemade with a gradual taper of 15 to 30 mm for capacities up to5 mL inclusive, and 20 to 40 mm for 10 to 50 mL capacitypipets. Style 2 pipets shall have a taper at a distance of 50 to 65mm from the tip end. The end of the tip shall be perpendicularto the longitudinal axis of the pip

10、et. A sudden constriction atthe orifice shall not be acceptable. The outside edge of the tipshall be beveled slightly at the end, and the bevel shall beground or firepolished.4.3 Zero Graduation Line Position The distance from thetop end to the top graduation of all pipets (except for 0.5 mLpipets)

11、shall be not less than 90 mm; for 0.5 mL pipets thisdistance shall be not less than 80 mm.4.4 Dimensions and Outflow TimesThe limiting dimen-sions and outflow times are shown in Table 1. Outflow timesshall be determined on unplugged pipets using distilled waterat 256 5C and by means of a stopwatch.

12、Outflow time shallbe determined by the unrestricted outflow of the water from thezero mark to base mark and until the water has ceased to flow.4.5 MarkingsAll markings shall be permanent and leg-ible.4.5.1 Graduation MarkingsGraduation lines shall not ex-ceed 0.4 mm in thickness and shall be in a pl

13、ane perpendicularto the vertical axis of the pipet. The lines and other markingsshall be applied by one of the following methods: etched andfilled with a permanent pigment; etched through a vertical1This specification is under the jurisdiction of ASTM Committee E41 onLaboratory Apparatus and is the

14、direct responsibility of Subcommittee E41.01 onApparatus.Current edition approved Nov. 1, 2007. Published January 2008. Originallyapproved in 1989. Last previous edition approved in 2002 as E 1293 02.2For referenced ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Servic

15、e at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.1Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.colored stripe fused into the glass; by applic

16、ation of a stainfired into the glass without etching; or by application of anenamel fired onto the glass without etching.4.5.1.1 Style 1, Class A pipetsShall comply with thelengths of lines, numbering and Graduation Pattern 1 specifiedin Specification E 694.4.5.1.2 Style 1, Class B and Style 2 pipet

17、sMain gradua-tions shall extend a minimum of34 of the way around the pipet;intermediate graduations shall extend a minimum of15 of theway around the pipet, and the least graduation markings shallextend a minimum of17 of the way around the pipet. The valueof all main graduations shall be inscribed in

18、 Arabic numeralsdirectly above the line referenced.4.5.2 Color CodingEach pipet shall be marked with oneor two color bands as specified in Table 1. For color code datasee Specification E 1273. Color bands do not have to becontinuous but may be broken as long as they cover 40 % ofthe circumference of

19、 the pipet. Colors must be bright anddistinct.4.5.3 Identification MarkingsEach pipet, marked by oneof the methods given in 4.5.1, shall show the manufacturersname or trademark, the nominal capacity, the temperature ofcalibration, 20C, and the word “delivers” or any of thefollowing symbols: TD, TD-E

20、X, TD (EX), EX-TD, EX (TD)or EX.5. Workmanship, Finish and Appearance5.1 Pipets shall be free of defects which may distort theappearance of the liquid surface or may impair their service-ability.6. General Requirements6.1 Borosilicate GlassBorosilicate glass for pipets shallconform to the glass requ

21、irements of Type 1, Class A or B ofSpecification E 438.6.2 AnnealingThe maximum residual thermal stress shallconform to Specification E 671, except that tempered tips ortempered tops, or both, may be supplied at the option of themanufacturer. The tempered tip, when examined in index oilthat matches

22、the refractive index of the glass being used, shallhave a temper between 75 and 220 nm for Style 1 tips, andbetween 40 and 200 nm for Style 2 tips.6.3 CalibrationPipets shall be calibrated in accordancewith Practice E 542, to deliver (TD) the intended capacity at20C.7. Sampling and Testing7.1 For sa

23、mpling and testing refer to Specification E 1157.8. Packaging8.1 For packaging select from Specifications E 920, E 921,or Practice E 1133.9. Keywords9.1 glass; measuring; pipetsTABLE 1 Requirements for Glass Measuring PipetsCapacitymLCapacity Tolerance6 mL Graduations, mL OutsideDiameter ofGrad. Por

24、tionmin, mmOutflow Time SecondsColorCodingBandClass A Class B Least valueMainnumberedClass A Class Bmin max min max0.1 . 0.005 0.01 0.01 5.5 . . 0.5 10 White0.1A. 0.005 0.001 0.01 5.5 . . 0.5 10 2-Green0.2 . 0.008 0.01 0.02 5.5 . . 0.5 8 Black0.2A. 0.008 0.001 0.02 5.5 . . 0.5 8 2-Blue0.5A. 0.01 0.0

25、1 0.10 6.0 . . 2 6 2-Yellow1.0 0.01 0.02 0.10 0.10 6.0 20 60 3 22 Red1.0 0.01 0.02 0.01 0.10 6.0 20 60 3 22 Yellow2.0 0.01 0.02 0.10 0.20 7.0 20 60 3 22 Green2.0A. 0.02 0.01 0.20 7.0 . . 3 22 2-White5.0 0.02 0.04 0.10 1.0 7.5 30 60 8 20 Blue10.0 0.03 0.06 0.10 1.0 9.5 30 60 8 30 Orange25.0B0.05 0.10

26、 0.10 1.0 12.0 40 70 15 35 White50.0B. 0.16 0.20 2.0 16.0 . . 20 40 BlackAStyle 2 only.BStyle 1 only.E 1293 02 (2007)2ASTM International takes no position respecting the validity of any patent rights asserted in connection with any item mentionedin this standard. Users of this standard are expressly

27、 advised that determination of the validity of any such patent rights, and the riskof infringement of such rights, are entirely their own responsibility.This standard is subject to revision at any time by the responsible technical committee and must be reviewed every five years andif not revised, ei

28、ther reapproved or withdrawn. Your comments are invited either for revision of this standard or for additional standardsand should be addressed to ASTM International Headquarters. Your comments will receive careful consideration at a meeting of theresponsible technical committee, which you may atten

29、d. If you feel that your comments have not received a fair hearing you shouldmake your views known to the ASTM Committee on Standards, at the address shown below.This standard is copyrighted by ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959,United States. Individual reprints (single or multiple copies) of this standard may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org).E 1293 02 (2007)3