1、BRITISH STANDARD BS1428-D4: 1963 (Also listed as BS797) Incorporating Amendment No.1 Specification for Capillary pipettes Microchemical apparatus Group D: Volumetric apparatusBS1428-D4:1963 This BritishStandard, having been approved by the LaboratoryApparatus IndustryStandards Committeeand endorsed
2、by theChairman of the ChemicalDivisional Council,waspublished undertheauthority of the General Councilon 26February1963 BSI12-1999 First published asBS797May1938 First revision asBS797 (BS1428-D4) January1954 Second revisionBS1428-D4 (BS797) February1963 The following BSI references relate to the wo
3、rk on this standard: Committee references LBC/11andLBC/11/2 Draft for comment A(LBC)16 ISBN 0 580 34148 8 Co-operating organizations The Laboratory Apparatus Industry Standards Committee, under whose supervision this BritishStandard was prepared, consists of representatives from the following Govern
4、ment departments and scientific and industrial organizations: Agricultural Research Council Air Ministry Association of British Chemical Manufacturers* Association of Scientific Workers Board of Trade British Association for the Advancement of Science British Chemical Ware Manufacturers Association*
5、 British Laboratory Ware Association* British Lampblown Scientific Glassware Manufacturers Association* British Pharmacopia Commission British Scientific Instrument Research Association* Chemical Society* D.S.I.R. Laboratory of the Government Chemist* D.S.I.R. National Chemical Laboratory* Glass Man
6、ufacturers Federation* Institute of Petroleum Ministry of Aviation* Ministry of Health National Physical Laboratory (D.S.I.R.)* Oil Companies Materials Association Pharmaceutical Society of Great Britain* Royal Institute of Chemistry* Science Masters Association Scientific Instrument Manufacturers A
7、ssociation Society for Analytical Chemistry* Society of Chemical Industry* Society of Glass Technology* Standardization of Tar Products Tests Committee The Government departments and scientific and industrial organizations marked with an asterisk in the above list, together with the following, were
8、directly represented on the committee entrusted with the preparation of this BritishStandard: Admiralty Association of Clinical Pathologists Medical Research Council Physiological Society War Office Individual manufacturers Amendments issued since publication Amd. No. Date of issue Comments 29 July1
9、968 Indicated by a sideline in the marginBS1428-D4:1963 BSI 12-1999 i Contents Page Co-operating organizations Inside front cover Foreword ii 1 Scope 1 2 Types and sizes 1 3 Definition of capacity 1 4 Tolerances on capacity 1 5 Material 1 6 Dimensions 3 7 Finish of top of pipette 3 8 Jet 3 9 Graduat
10、ion lines 3 10 Inscriptions 3 11 Colour coding 4 Appendix A Determination of capacity 5 Appendix B Testing of BritishStandards capillary pipettes 6 Appendix C Parts of BS1428 Microchemical apparatus 6 Figure 1 Type1 capillary pipettes 7 Figure 2 Type2 capillary pipettes 8 Figure 3 Type3 capillary pi
11、pettes 9 Figure 4 Type4 capillary pipettes 9 Figure 5 Jets for capillary pipettes 9 Table 1 Tolerances on capacity 1 Table 2 Mandatory dimensions 2 Table 3 Recommended overall length 3BS1428-D4:1963 ii BSI 12-1999 Foreword This standard makes reference to the following BritishStandard: BS1797, Table
12、s for use in the calibration of volumetric glassware. This BritishStandard was first published in1938 as BS797, “One-mark capillary pipettes”, the1954 revision was published under the same number but in order to include it also in the BS1428, “Microchemical apparatus” series (seelist in Appendix C),
13、 it was allotted the additional number BS1428-D4. A further step has now been taken and first place given to this latter number, the original number, BS797, being relegated to the status of a subsidiary reference. In this revision a further category of two-mark content pipettes (Type4) has been adde
14、d and several changes in dimensions have been made. All the dimensions are now mandatory except for the overall lengths of the pipettes which are recommended for the guidance of manufacturers. Because of the desirability that Types3 and4 pipettes of capacity0.02ml and above should be suitable for us
15、e with both aqueous solutions and biological fluids such as blood, a relatively narrow range of jet diameter is permitted for these pipettes. All the one-mark pipettes may now be made from soda-lime or borosilicate glass. Enamel-back tubing and lead glass may also be used. In preparing this revision
16、, account has been taken of International Standardization work in the field of laboratory glassware and this has led to the inclusion of a subsidiary standard temperature of27 C for the convenience of tropical countries and to the use of the inscription “Ex” in place of “D” to indicate that the pipe
17、tte is to be used to deliver, or the inscription “In” instead of “C” to indicate that the pipette is to be used to contain. Appendix A describes methods of determining capacity using water for pipettes of Types2,3 and4, and a method using mercury for pipettes of Type3. A British Standard does not pu
18、rport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front
19、 cover, pagesi andii, pages1 to9 and a back cover. This standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover.BS1428-D4:1963 BSI 12-1999 1 1 Scope This BritishStandard specifies ranges of gradu
20、ated, one-mark and two-mark capillary pipettes suitable for general laboratory use. 2 Types and sizes Four types of capillary pipette are specified, as follows: Type1. Graduated pipettes calibrated for delivery from the zero line down to any graduation line as illustrated in Figure 1. Three sizes ar
21、e specified, as follows: Nominal capacity0.1ml, subdivided into0.005ml intervals; Nominal capacity0.2ml, subdivided into0.01ml intervals; Nominal capacity0.5ml, subdivided into0.02ml intervals. Type2. Graduated pipettes calibrated for content from the jet to any graduation line, as illustrated in Fi
22、gure 2. Three sizes are specified, as follows: Nominal capacity0.1ml, subdivided into0.005ml intervals; Nominal capacity0.2ml, subdivided into0.01ml intervals; Nominal capacity0.5ml, subdivided into0.02ml intervals. Type3. One-mark pipettes calibrated for content, as illustrated in Figure 3. Four si
23、zes are specified, of nominal capacity0.005,0.01,0.02 and0.05ml. Type4. Two-mark pipettes calibrated for content, as illustrated in Figure 4. Two sizes are specified, of nominal capacity0.2ml, subdivided into0.1ml intervals, and0.1ml, subdivided into0.05ml intervals. 3 Definition of capacity 3.1 pip
24、ettes of type1 the capacity corresponding to any graduation line is defined as the volume of water at20 C, expressed in millilitres, delivered by the pipette at20 C, when emptied from the zero line to that graduation line, as described in Appendix A 3.2 pipettes of types2,3 and4 the capacity corresp
25、onding to any graduation line is defined as the volume of water at20 C, expressed in millilitres, contained by the pipette at20 C, when filled from the jet to that graduation line, the lowest point of the water meniscus being set in the horizontal plane containing the top edge of the graduation line
26、, with the pipette vertical. The capacity can be determined by the methods described in Appendix A NOTEWhen a pipette of any type is required for use in a country which has adopted a standard temperature of27 C, this figure is to be substituted for20 C. 4 Tolerances on capacity Tolerances on capacit
27、y for all the pipettes shall be as shown in Table 1. The tolerance represents the maximum error allowed at any point and also the maximum difference allowed between the errors at any two points. 5 Material All capillary pipettes shall be made from either soda-lime or borosilicate glass. One-mark and
28、 two-mark pipettes shall be made from either clear or enamel-back glass tubing; other pipettes shall be made from clear glass tubing. In either case, the glass shall be as free as possible from visible defects and shall be well annealed. The manufacturer shall state the type of glass used. Table 1 T
29、olerances on capacity Nominal capacity ofpipette, ml 0.005 0.01 0.02 0.05 0.1 0.2 0.5 Tolerance, ml 0.0002 0.0002 0.0004 0.001 0.002 0.004 0.006BS 1428-D4:1963 2 BSI 12-1999 Table 2 Mandatory dimensions All dimensions are in millimetres except where otherwise stated Dimensions Type1 Delivery (gradua
30、ted) Type2 Content (graduated) Type3 Content (one-mark) Type4 Content (two-mark) 0.1ml 0.2ml 0.5ml 0.1ml 0.2ml 0.5ml 0.005ml 0.01ml 0.02ml 0.05ml 0.1ml 0.2ml Distance from top of pipette to nearest graduation line min. 80 80 80 80 80 80 80 40 40 50 40 40 Distance from highest to lowest numbered line
31、 70 to120 70 to120 100 to150 63 to108 53 to90 80 to120 Distance from lowest graduation line to tip of jet 30 to35 20 to25 20 to25 Distance from graduation line to tip of jet 20 to40 40 to80 50 to80 60 to100 Distance from highest graduation line to tip of jet 70 to160 70 to160 External diameter 5 to6
32、 5 to6 5 to6 5 to6 5 to6 5 to6 5 to6 5 to6 5 to6 5 to6 5 to6 5 to6 External diameter at top of bevel 2.0 to3.5 2.0 to3.5 2.0 to3.5 2.0 to3.5 2.0 to3.5 2.0 to3.5 2.0 to3.5 2.0 to3.5 2.0 to3.5 2.0 to3.5 Internal diameter at tip 0.3 to0.5 0.3 to0.5 0.3 to0.5 0.3 to0.5 0.3 to0.5 0.3 to0.5 0.3 to0.5 0.3
33、to0.5 0.4 to0.55 0.4 to0.55 0.4 to0.55 0.4 to0.55 Wall thickness at tip of jet 0.25 to0.50 0.25 to0.50 0.25 to0.50 0.25 to0.50 0.25 to0.50 0.25 to0.50 0.25 to0.50 0.25 to0.50 0.25 to0.50 0.25 to0.50 0.25 to0.50 0.25 to0.50 Included angle of bevel 35 to45 35 to45 35 to45 35 to45 35 to45 35 to45 35 to
34、45 35 to45 35 to45 35 to45 Included angle of taper 10 to25 10 to25 10 to25 10 to25 10 to25 10 to25 25 to30 25 to30 10 to25 10 to25 10 to25 10 to25BS1428-D4:1963 BSI 12-1999 3 6 Dimensions The pipettes shall comply with the dimensions given in Table 2. It is recommended that the overall lengths of th
35、e pipettes should comply with the dimensions given in Table 3. Table 3 Recommended overall length 7 Finish of top of pipette The top of the pipette shall be finished at right angles to the axis of the pipette and shall be free from any blemishes that would interfere with the required accurate contro
36、l by the finger in setting the meniscus. The top1 or2cm of the pipette may taper slightly to facilitate the attachment of rubber tubing. 8 Jet For one-mark pipettes of nominal capacity less than0.02ml the jet shall consist of a tapered portion formed by grinding, as shown in Figure 5a. There shall b
37、e no sudden constriction of the bore at the orifice. For pipettes of nominal capacity0.02ml and above of all types, the jet shall consist of a tapered portion formed by drawing and a bevel formed by grinding, as shown in Figure 5b. The tapered portion may be slightly concave. There shall be no sudde
38、n constriction of the bore at the orifice. For all pipettes, the end of the jet shall be ground smooth at right angles to the axis of the pipette and the tapered portion of the jet shall be polished. 9 Graduation lines The graduation lines shall be clean permanent lines lying in planes at right angl
39、es to the axis of the pipettes. For graduated pipettes the lines shall be of uniform thickness and evenly spaced. The lowest graduation line shall be above the tapered portion of the jet. The numbered graduation lines shall extend completely round the circumference of the pipette; the length of the
40、shorter lines shall be approximately one-sixth of the circumference of the pipette. The scale shall be divided and numbered as shown in Figure 1 and Figure 2. For one-mark and two-mark pipettes each graduation line shall extend completely round the circumference of the pipettes except in the case of
41、 enamel-back pipettes where it shall extend over at least the clear portion of the circumference of the pipette. The numerical value of the nominal capacity, e.g.0.05, shall be marked immediately above each graduation line. 10 Inscriptions The following inscriptions shall be permanently and legibly
42、marked on all capillary pipettes. a) The abbreviation “ml” to indicate the unit in terms of which the pipette is calibrated. b) The abbreviation20 C to indicate the standard temperature. NOTEWhere the pipette is required for use in a country which has adopted a standard temperature of27 C, this figu
43、re is to be substituted for20 C. c) The letters “Ex” (for pipettes of Type1) to indicate that the pipette is to be used “to deliver” or the letters “In” (for pipettes of Types2,3 and4) to indicate that it is to be used “to contain”. d) The makers or vendors name or mark. Type and nominal capacity Ov
44、erall length Type1. Delivery (graduated) 0.1,0.2,0.5ml Type2. Content (graduated) 0.1,0.2,0.5ml Type3. Content (one-mark) 0.005,0.01,0.02ml 0.05ml Type4. Content (two-mark) 0.1,0.2ml 270 240 120 150 200BS1428-D4:1963 4 BSI 12-1999 e) The number of this BritishStandard, i.e.“BS1428” 1) . f) If requir
45、ed, an identification number. 11 Colour coding If a colour coding system is used to identify the nominal capacity and unit of subdivision of a pipette complying with this BritishStandard, such coding shall comply with the appropriate provisions of BS3996 2) . 1) The mark BS1428 on the product is a c
46、laim by the manufacturer that it complies with the requirements of the standard. The BritishStandards Institution is the owner of the registered certification trade mark shown below. This mark can be used only by manufacturers licensed under the certification mark scheme operated by the BSI. The pre
47、sence of this mark on a product is an assurance that the goods have been produced to comply with the requirements of the BritishStandard under a system of supervision, control and testing operated during manufacture and including periodical inspections at the manufacturers works in accordance with t
48、he certification mark scheme operated by the BSI. Further particulars of the terms of licence may be obtained from the Director, BritishStandards Institution,2Park Street, London,W.1. 2) BS3996, “Colour coding for one-mark and graduated pipettes.”BS1428-D4:1963 BSI 12-1999 5 Appendix A Determination
49、 of capacity Type1. When determining the capacity corresponding to any graduation line of a pipette of Type1, the following procedure is to be observed, the pipette having first been thoroughly cleaned. The pipette is clamped in a vertical position with the jet downwards, filled with distilled water to a short distance above the zero line, and the water is retained in the pipette by pressing a finger
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