BS EN 1899-1-1998 Water quality - Determination of biochemical oxygen demand after n days (BODn) - Dilution and seeding method with allylthiourea addition《水质 数天后生化需氧的测定 加入烯丙基硫醇的稀释和.pdf

1、 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | BRITISH STANDARD BS EN 1899-1:1998 BS 6068

2、2.63: 1998 The European Standard EN 1889-1:1998 has the status of a British Standard ICS 13.060.01; 13.060.30 NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAW Water quality Determination of biochemical oxygen demand after n days (BOD n ) Part 1: Dilution and seeding method wit

3、h allylthiourea additionThis British Standard, having been prepared under the direction of the Health and Environment Sector Committee was published under the authority of the Standards Committee and comes into effect on 15 November 1998 BSI 1998 ISBN 0 580 29680 6 BS EN 1899-1:1998 Amendments issue

4、d since publication Amd. No. Date Text affected National foreword This British Standard is the English language version of EN 1899-1:1998. It supersedes BS 6068-2.14:1990 which is withdrawn. The UK participation in its preparation was entrusted by Technical Committee EH/3, Water quality, to Subcommi

5、ttee EH/3/2, Physical, chemical and biochemical methods, which has the responsibility to: aid enquirers to understand the text; present to the responsible European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related internationa

6、l and European developments and promulgate them in the UK. A list of organizations represented on this subcommittee can be obtained on request to its secretary. BS EN 1899-1 is one of a series of standards on water quality, others of which have been, or will be, published as Sections of BS 6068. Thi

7、s standard has therefore been given the secondary identifier BS 6068-2.63. The various Sections of BS 6068 are comprised within Parts 1 to 7, which, together with Part 0, are listed below. Part 0 Introduction Part 1 Glossary Part 2 Physical, chemical and biochemical methods Part 3 Radiological metho

8、ds Part 4 Microbiological methods Part 5 Biological methods Part 6 Sampling Part 7 Precision and accuracy NOTE The tests described in this British Standard should only be carries out by suitably qualified persons with an appropriate level of chemical expertize. Standard chemical procedures should be

9、 followed throughout. Further information Users of this standard are informed that the UK submitted a vote of disapproval on the final voting stage of the draft EN for the following reasons. Although we are in general technical agreement with the basis of the draft, we are still submitting a negativ

10、e vote as this standard contains two methods. We therefore consider that it should be published as two separate standards, i.e a 5 day one and a 7 day one. This would thereby avoid any possible confusion when quoting the standard or reporting the results of analysis. We also strongly recommend delet

11、ion of the reference to ISO 5815:1989 in the title as it serves no purpose and could be misleading. The ISO standard is for 5 days only and it is thus wrong to claim in the Foreword that it has been approved with common modifications with no reference to the major change in test conditions. Cross-re

12、ferences The British Standards which implement international or European publications referred to in this document may be found in the BSI Standards Catalogue under the section entitled International Standards Correspondence Index, or by using the Find facility of the BSI Standards Electronic Catalo

13、gue. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document compr

14、ises a front cover, an inside front cover, page 1 to 12, an inside back cover and a back cover.CEN European Committee for Standardization Comite Europe en de Normalisation Europa isches Komitee fu r Normung Central Secretariat: rue de Stassart 36, B-1050 Brussels 1998 CEN All rights of exploitation

15、in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 1899-1:1998 E EUROPEAN STANDARD EN 1899-1 NORME EUROPE ENNE EUROPA ISCHE NORM March 1998 ICS 13.060.01 Descriptors: water tests, water, chemical analysis, determination of content, biochemical oxygen demand, diluti

16、on English version Water quality Determination of biochemical oxygen demand after n days (BOD n ) Part 1: Dilution and seeding method with allylthiourea addition (ISO 5815:1989, modified) Qualite de leau De termination de la demande biochimique en oxyge ne apre s n jours (DOB n ) Partie 1: Me thode

17、par dilution et ensemencement avec apport dallyl thio-ure e (ISO 5815:1989, modifie e) Wasserbeschaffenheit Bestimmung des Biochemischen Sauerstoffbedarfs nach n Tagen (BSB n ) Teil 1: Verdu nnungs- und Impfverfahren nach Zugabe von Allylthioharnstoff (ISO 5815:1989, modifiziert) This European Stand

18、ard was approved by CEN on 13 February 1998. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning

19、 such national standards may be obtained on application to the Central Secretariat or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own langu

20、age and notified to the Central Secretariat has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, S

21、witzerland and United Kingdom.Page 2 EN 1899-1:1998 BSI 1998 Foreword This European Standard has been prepared by Technical Committee CEN/TC 230, Water analysis, the Secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of

22、 an identical text or by endorsement, at the latest by September 1998, and conflicting national standards shall be withdrawn at the latest by September 1998. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this

23、 European standard: Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the United Kingdom. This European Standard consists of the following parts: EN 1899-1, Water quality Det

24、ermination of biochemical oxygen demand after n days (BOD n ) Part 1: Dilution and seeding method with allylthiourea addition. EN 1899-2, Water quality Determination of biochemical oxygen demand after n days (BOD n ) Part 2: Method for undiluted samples. Annex A, which is normative, concerns alterna

25、tive incubation periods. Annex B, which is informative, concerns multitesting, which may be used to obtain enhanced precision, or if the presence of substances toxic to microorganisms is suspected. Endorsement notice The text of the International Standard ISO 5815:1989 was approved by CEN as a Europ

26、ean Standard with agreed common modifications as given below: change in setup (splitting into two parts) addition of annexes. Contents Page Foreword 2 Introduction 3 1 Scope 3 2 Normative references 3 3 Definition 4 4 Principle 4 5 Reagents 4 6 Apparatus 5 7 Storage of the sample 5 8 Procedure 5 9 C

27、alculation and expression of results 7 10 Trueness and precision 8 11 Special cases 8 12 Test report 8 Annex A (normative) Alternative incubation periods 11 Annex B (informative) Multitesting 12Page 3 EN 1899-1:1998 BSI 1998 Introduction This European Standard EN 1899-1 is a modified version of ISO

28、5815:1989, Water quality Determination of biochemical oxygen demand after 5 days (BOD 5 ) Dilution and seeding method. The times of incubation specified in the proposal are 5 days - as in ISO 5815 and as has been applied in many European countries - or 7 days as has been applied in several Nordic co

29、untries through the past many years. The 7 day incubation will typically give higher BOD results than 5 days incubation time. With an incubation time of 5 days, weekend work can only be avoided if samples are collected Wednesdays, Thursdays to Fridays. With an incubation time of 7 days, samples coll

30、ected on the first five weekdays can be analysed without implying weekend work. For this reason a 7 day incubation can be considered more convenient than the conventional 5 day incubation. A new, modified 7 day incubation is described in a normative annex. The first investigations indicate that BOD

31、results obtained by this modified method are identical to results obtained by the 5 day method described in the proposed standard. It is hoped that more comparative data on these two incubation methods will be obtained during the coming years, so that the modified 7 day incubation method can be incl

32、uded fully at the time of review and revision of this standard. 1 Scope This European Standard specifies a determination of the biochemical oxygen demand of waters by dilution and seeding with suppression of nitrification. This standard is applicable to all waters having biochemical oxygen demands g

33、reater than or equal to the limit of determination 3 mg/l of oxygen and not exceeding 6 000 mg/l of oxygen. For biochemical oxygen demands greater than 6 000 mg of oxygen/l, the method is still applicable, but the errors caused by the necessary dilutions can influence the analytical quality of the t

34、est method and the results are to be interpreted with circumspection. In this standard the limit of detection, D L , is defined as D L = t 0,95(f) 2 s B (1) 1+ 1 n where s B is the within series standard deviation,t 0,95(f) is the student t-value, with f is the degrees of freedom for the determinati

35、on of s B and n is the number of analysis for determination of the blank in an analytical series. s B is calculated from determinations of real samples with a BOD content near the estimated D L . In cases where the analytical method does not require any blank correction the term (2) 1+ 1 n is omitte

36、d. The results obtained are the product of a combination of biochemical and chemical reactions. They do not have the rigorous and unambiguous character of those resulting from, for example, a single, well-defined, chemical process. Nevertheless, they provide an indication from which the quality of w

37、aters can be estimated. The test can be influenced by the presence of various substances. Those which are toxic to microorganisms, for example bactericides, toxic metals or free chlorine, will inhibit biochemical oxidation. The presence of algae or nitrifying microorganisms can produce artificially

38、high results. It is absolutely essential that tests conducted according to this standard are carried out by suitably qualified staff. Annex A describes alternative incubation periods. Annex B describes multitesting, which can be used to obtain enhanced precision or to demonstrate the presence of sub

39、stances toxic to microorganisms. 2 Normative references This European Standard incorporates by dated or undated reference provisions from other publications. These normative references are cited at the appropriate places in the text and the publications are listed hereafter. For dated references, su

40、bsequent amendments to or revisions of any of these publications apply to this European Standard only when incorporated in it by amendment or revision. For undated references the latest edition of the publication referred to applies. EN ISO 3696, Water for analytical laboratory use Specification and

41、 test methods (ISO 3696:1987). EN 25813, Water quality Determination of dissolved oxygen Iodometric method (ISO 5813:1983). EN 25814, Water quality Determination of dissolved oxygen Electrochemical probe method (ISO 5814:1990). EN ISO 8467, Water quality Determination of permanganate index (ISO 8467

42、1993). ISO 6060:1989, Water quality Determination of chemical oxygen demand. ISO 6107-2:1997, Water quality Vocabulary Part 2. ISO 7393-1:1985, Water quality Determination of free chlorine and total chlorine Part 1: Titrimetric method using N,N-diethyl-1,4-phenylenediamine. ISO 7393-2:1985, Water q

43、uality Determination of free chlorine and total chlorine Part 2: Colorimetric method using N,N-diethyl-1,4-phenylenediamine, for routine control purposes. ISO 8245:1987, Water quality Guidelines for the determination of total organic carbon (TOC).Page 4 EN 1899-1:1998 BSI 1998 3 Definition For the p

44、urposes of this European Standard, the following definition applies: biochemical oxygen demand after n days (BOD n ) the mass concentration of dissolved oxygen consumed under specified conditions by the biochemical oxidation of organic and/or inorganic matter in water. n is the incubation time; it i

45、s equal to 5 or 7 NOTE This definition is similar to the definition of ISO 6107-2. For the purpose of this European Standard, biochemical oxidation is taken to mean “biological oxidation” 4 Principle Pre-treatment of the sample of water to be analysed and dilution with varying amounts of a dilution

46、water rich in dissolved oxygen and containing a seed of aerobic microorganisms, with suppression of nitrification. Incubation at 208C for a defined period, 5 or 7 days, in the dark, in a completely filled and stoppered bottle. Determination of the dissolved oxygen concentration before and after incu

47、bation. Calculation of the mass of oxygen consumed per litre of sample. 5 Reagents 5.1 General Throughout the text, use only reagents of recognized analytical quality. 5.2 Water Use only grade 3 water (in accordance with EN ISO 3696). However, the water shall not contain more than 0,01 mg/l of coppe

48、r, nor chlorine or chloramines. 5.3 Seeding water If the test sample does not contain, by itself, sufficient adapted microorganisms, seeding water, obtained in one of the following ways, shall be used: a) Urban waste water of maximum COD (chemical oxygen demand measured in accordance with ISO 6060)

49、300 mg/l or TOC (total organic carbon measured in accordance with ISO 8245) 100 mg/l, collected from a main sewer or from a sewer of a residential zone free from significant industrial contamination. Decant or filter the water through a coarse filter; b) River or lake water containing urban waste water; c) Settled effluent from a waste water treatment plant; d) Water taken downstream from the discharge of the water to be analysed or water containing microorganisms adapted t