1、 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | BRITISH STANDARD BS EN 1899-2:1998 BS 6068
2、2.64: 1998 The European Standard EN 1889-2:1998 has the status of a British Standard ICS 13.060.01; 13.060.30 NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAW Water quality Determination of biochemical oxygen demand after n days (BOD n ) Part 2: Method for undiluted samplesThi
3、s British Standard, having been prepared under the direction of the Health and Environment Sector Committee was published under the authority of the Standards Committee and comes into effect on 15 November 1998 BSI 1998 ISBN 0 580 29685 7 BS EN 1899-2:1998 Amendments issued since publication Amd. No
4、 Date Text affected National foreword This British Standard is the English language version of EN 1899-2:1998. Together with BS EN 1899-1:1998 it supersedes BS 6068-2.14:1990 which is withdrawn. The UK participation in its preparation was entrusted by Technical Committee EH/3, Water quality, to Sub
5、committee EH/3/2, Physical, chemical and biochemical methods, which has the responsibility to: aid enquirers to understand the text; present to the responsible European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related interna
6、tional and European developments and promulgate them in the UK. A list of organizations represented on this committee can be obtained on request to its secretary. BS EN 1899-1 is one of a series of standards on water quality, others of which have been, or will be, published as Sections of BS 6068. T
7、his standard has therefore been given the secondary identifier BS 6068-2.63. The various Sections of BS 6068 are comprised within Parts 1 to 7, which, together with Part 0, are listed below. Part 0 Introduction Part 1 Glossary Part 2 Physical, chemical and biochemical methods Part 3 Radiological met
8、hods Part 4 Microbiological methods Part 5 Biological methods Part 6 Sampling Part 7 Precision and accuracy NOTE The tests described in this British Standard should only be carried out by suitably qualified persons with an appropriate level of chemical expertise. Standard chemical procedures should
9、be followed throughout. Further information Users of this standard are informed that the UK submitted a vote of disapproval on the final voting stage of the draft EN for the following reasons: Although we are in general technical agreement with the basis of the draft, we are still submitting a negat
10、ive vote as this standard contains two methods. We therefore consider that it should be published as two separate standards, i.e a 5 day one and a 7 day one. This would thereby avoid any possible confusion when quoting the standard or reporting the results of analysis. We also strongly recommend del
11、etion of the reference to ISO 5815:1989 in the title as it serves no purpose and could be misleading. The ISO standard is for 5 days only and it is thus wrong to claim in the Foreword that it has been approved with common modifications with no reference to the major change in test conditions. Cross-
12、references The British Standards which implement international or European publications referred to in this document may be found in the BSI Standards Catalogue under the section entitled International Standards Correspondence Index, or by using the Find facility of the BSI Standards Electronic Cata
13、logue. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document com
14、prises a front cover, an inside front cover, page 1 to 10, an inside back cover and a back cover.CEN European Committee for Standardization Comite Europe en de Normalisation Europa isches Komitee fu r Normung Central Secretariat: rue de Stassart, 36 B-1050 Brussels 1998 CEN All rights of exploitatio
15、n in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 1899-2:1998 E EUROPEAN STANDARD EN 1899-2 NORME EUROPE ENNE EUROPA ISCHE NORM March 1998 ICS 13.060.01 Descriptors: water tests, water, chemical analysis, determination of content, oxygen demand, samples English
16、version Water quality Determination of biochemical oxygen demand after n days (BOD n ) Part 2: Method for undiluted samples (ISO 5815:1989, modified) Qualite de leau De termination de la demande biochimique en oxyge ne apre s n jours (DOB n ) Partie 2: Me thode pour les e chantillons non dilue s(ISO
17、 5815:1989, modifie e) Wasserbeschaffenheit Bestimmung des Biochemischen Sauerstoffbedarfs nach n Tagen (BSB n ) Teil 2: Verfahren fu r unverdu nnte Proben (ISO 5815:1989, modifiziert) This European Standard was approved by CEN on 22 February 1998. CEN members are bound to comply with the CEN/CENELE
18、C Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the Central Secretariat or to any CEN
19、 member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official versions. CEN me
20、mbers are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.Page 2 EN 1899-2:1998 BSI 1998 Foreword This European Standard has
21、 been prepared by Technical Committee CEN/TC 230, Water analysis, the Secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by September 1998, and conflicting national
22、standards shall be withdrawn at the latest by September 1998. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European standard: Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
23、 Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the United Kingdom. This European Standard consists of the following parts: EN 1899-1, Water quality Determination of biochemical oxygen demand after n days (BOD n ) Part 1: Dilution and seeding metho
24、d with allylthiourea addition. EN 1899-2, Water quality Determination of biochemical oxygen demand after n days (BOD n ) Part 2: Method for undiluted samples. Annex A, which is normative, concerns alternative incubation periods. Annex B, which is normative, concerns modifications for specific evalua
25、tions of the quality of waters. Annex C, which is informative, contains a bibliography. Endorsement notice The text of the International standard ISO 5815:1989 was approved by CEN as a European Standard with agreed common modifications as given below: change in setup (splitting into two parts) addit
26、ions of annexes. Contents Page Foreword 2 Introduction 3 1 Scope 3 2 Normative references 3 3 Definition 4 4 Principle 4 5 Apparatus 4 6 Storage of the sample 4 7 Procedure 4 8 Calculation and expression of results 5 9 Trueness and precision 5 10 Test report 5 Annex A (normative) Alternative incubat
27、ion periods and temperatures 8 Annex B (normative) Modifications for specific evaluations 9 Annex C (informative) Bibliography 10Page 3 EN 1899-2:1998 BSI 1998 Introduction This European Standard EN 1899-2 is intended for analysis of BOD in waters with a BOD in the range (0,5 to 6) mg/l of oxygen. T
28、he times of incubation specified in the proposal are 5 days - as in ISO 5815:1989, Water quality Determination of biochemical oxygen demand after 5 days (BOD 5 ) Dilution and seeding method. Incubation for 5 days - as has been applied in many European countries - or 7 days as has been applied in sev
29、eral Nordic countries through the past many years. The 7 day incubation will typically give higher BOD results than 5 days incubation time. With an incubation time of 5 days, weekend work can only be avoided if samples are collected Wednesdays, Thursdays to Fridays. With an incubation time of 7 days
30、 samples collected on the first five weekdays can be analysed without implying weekend work. For this reason a 7 day incubation can be considered more convenient than the conventional 5 day incubation. A modified 7 day incubation is described in a normative annex. The first investigations indicate
31、that BOD results obtained by this modified method are identical to results obtained by the 5 day method described in the proposed standard. It is hoped that more comparative data on these two incubation methods will be obtained during the coming years, so that the modified 7 day incubation method ca
32、n be included fully at the time of review and revision of this standard. 1 Scope This European Standard specifies determination of the biochemical oxygen demand of waters of undiluted samples. This standard is applicable to all waters having biochemical oxygen demands greater than or equal to the li
33、mit of determination 0,5 mg/l of oxygen and not exceeding 6 mg/l of oxygen. In this standard, the limit of determination, D L , is defined as D L = t 0,95(f) 2s B (1) 1+ 1 n where s B is the within series standard deviation, t 0,95(f) is the student t-value, with f is the degrees of freedom for the
34、determination of s B and n is the number of analysis for determination of the blank in an analytical series. s B is calculated from determinations of real samples with a BOD contentration near the estimated D L . In cases where the analytical method does not require any blank correction, the term (2
35、) 1+ 1 n is omitted. The results obtained are the product of a combination of biochemical and chemical reactions. They do not have the rigorous and unambiguous character of those resulting from, for example, a single, well-defined, chemical process. Nevertheless, they provide an indication from whic
36、h the quality of waters can be estimated. The test can be influenced by the presence of various substances. Those which are toxic to microorganisms, for example bactericides, toxic metals or free chlorine, will inhibit biochemical oxidation. The presence of algae or nitrifying microorganisms can pro
37、duce high results. In these situations a modification of the method (see annex B or as described in EN 1899-1). It is absolutely essential that tests conducted according to this standard are carried out by suitably qualified staff. Annex A describes alternative incubation periods. Annex B describes
38、procedures for modification of the method by addition of seeding material, salts, inhibition of nitrification by allylthiourea (ATU) addition, neutralization, homogenization and/or filtration. These modifications may be found necessary for specific evaluations of the water quality of receiving water
39、s. 2 Normative references This European Standard incorporates by dated or undated reference provisions from other publications. These normative references are cited at the appropriate places in the text and the publications are listed hereafter. For dated references, subsequent amendments to or revi
40、sions of any of these publications apply to this European Standard only when incorporated in it by amendment or revision. For undated references the latest edition of the publication referred to applies. EN ISO 3696, Water for analytical laboratory use Specification and test methods (ISO 3696:1987).
41、 EN 25813, Water quality Determination of dissolved oxygen Iodometric method (ISO 5813:1983). EN 25814, Water quality Determination of dissolved oxygen Electrochemical probe method (ISO 5814:1990). ISO 6107-2:1997, Water quality Vocabulary Part 2.Page 4 EN 1899-2:1998 BSI 1998 3 Definition For the p
42、urposes of this European Standard, the following definition applies: biochemical oxygen demand after n days (BOD n ) the mass concentration of dissolved oxygen consumed under specified conditions by the biochemical oxidation of organic and/or inorganic matter in water. n is the incubation time; it i
43、s equal to 5 or 7. NOTE For the purpose of this European Standard, “biochemical oxidation” is taken to mean “biological oxidation”. This definition is similar to the definition of ISO 6107-2. 4 Principle Equilibration of the sample of water to be analyzed to 208C followed by, if necessary, aeration.
44、 Incubation at 208C for a defined period, 5 or 7 days, in the dark, in a completely filled and stoppered bottle. Determination of the dissolved oxygen concentration before and after incubation. Calculation of the mass of oxygen consumed per litre of sample. 5 Apparatus 5.1 Incubation bottles, BOD bo
45、ttles, with glass stoppers, For example, preferably 250 ml to 300 ml or 100 ml to 125 ml, with stoppers and preferably with straight shoulders, or any equivalent bottles. It is important that the bottles are thoroughly cleaned before use. If the iodometric method (in accordance with EN 25813) for de
46、termining dissolved oxygen is used, it is, for example, normally sufficient to rinse the bottle several times with tap water then deionized water. However, if the electrode method (in accordance with EN 25814) is used, a more stringent cleaning procedure, for example, as follows, is required. Add to
47、 the empty bottle 5 ml to 10 ml of a wash solution (for example 2,5 g of iodine plus 12,5 g of potassium iodide per litre of 1 % (V/V) sulfuric acid) shaking well to coat the bottle walls. Let stand for 15 min, pour off the solution and rinse thoroughly with tap water and finally deionized water. 5.
48、2 Incubator, capable of being maintained at (20 1)8C. 5.3 Equipment for determining dissolved oxygen concentration, in accordance with EN 25813 and EN 25814. 5.4 Means of refrigeration,( 0t o4 )8 C, for transport and storage of the sample. 5.5 Aeration equipment, a bottle of compressed air or a comp
49、ressor: The air quality shall be such that the aeration does not lead to any contaminations, especially by the addition of organic matter, oxidizing or reducing materials, or metals. If contamination is suspected, the air shall be filtered or washed. 6 Storage of the sample Store the sample at a temperature (0 to 4)8C in a filled and hermetically stoppered bottle immediately after sample collection and until the analysis is performed. Begin the determination of the BOD n as soon as
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