1、BRITISH STANDARD BS EN 370:1993 Wood preservatives Determination of eradicant efficacy in preventing emergence of Anobium punctatum (De Geer) The European Standard EN370:1993 has the status of a BritishStandard UDC 74.048.4:620.193.87BSEN370:1993 This BritishStandard, having been prepared under the
2、directionof the Technical SectorBoard for Building andCivil Engineering, waspublished under the authorityofthe Standards Board and comes into effect on 15May1993 BSI12-1999 The following BSI references relate to the work on this standard: Committee reference B/515 Draft for comment90/54561 DC ISBN 0
3、 580 21420 6 Cooperating organizations The European Committee for Standardization (CEN), under whose supervision this European Standard was prepared, comprises the national standards organizations of the following countries: Austria Oesterreichisches Normungsinstitut Belgium Institut belge de normal
4、isation Denmark Dansk Standardiseringsraad Finland Suomen Standardisoimisliito, r.y. France Association franaise de normalisation Germany Deutsches Institut fr Normung e.V. Greece Hellenic Organization for Standardization Iceland Technological Institute of Iceland Ireland National Standards Authorit
5、y of Ireland Italy Ente Nazionale Italiano di Unificazione Luxembourg Inspection du Travail et des Mines Netherlands Nederlands Normalisatie-instituut Norway Norges Standardiseringsforbund Portugal Instituto Portugus da Qualidade Spain Asociacin Espaola de Normalizacin y Certificacin Sweden Standard
6、iseringskommissionen i Sverige Switzerland Association suisse de normalisation United Kingdom BritishStandards Institution Amendments issued since publication Amd. No. Date CommentsBSEN370:1993 BSI 12-1999 i Contents Page Cooperating organizations Inside front cover National foreword ii Foreword 2 T
7、ext of EN370 3 National annex NA (informative) Committees responsible Inside back cover National annex NB (informative) Cross-references Inside back coverBSEN370:1993 ii BSI 12-1999 National foreword This BritishStandard has been prepared under the direction of the Technical Sector Board for Buildin
8、g and Civil Engineering and is the English language version of EN370:1993 Wood preservatives Determination of eradicant efficacy in preventing emergence of Anobium punctatum (De Geer), published by the European Committee for Standardization (CEN). EN370:1993 was produced as a result of international
9、 discussion in which the United Kingdom took an active part. CAUTION. Attention is drawn to the Health and Safety at Work etc. Act1974, and a need for ensuring that the method specified in this BritishStandard is carried out with suitable precautions. The procedure described in this BritishStandard
10、is intended to be carried out by appropriately qualified and experienced persons or other suitably trained and/or supervised personnel. Attention is drawn to the precautions given in the introduction and5.3.2. A British Standard does not purport to include all the necessary provisions of a contract.
11、 Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, pagesi andii, theEN title page, pages2 to14, an insi
12、de back cover and a back cover. This standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover.EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN370 April1993 UDC 74.048.4:620.193.87 Descriptors:
13、 Wood, wood preservatives, insecticides, pesticides, pest control, laboratory tests, determination, effectiveness, anobiidae English version Wood preservatives Determination of eradicant efficacy in preventing emergence of Anobium punctatum (De Geer) Produits de prservation du bois Dtermination de l
14、efficacit curative contre lmergence dAnobium punctatum (De Geer) Holzschutzmittel Bestimmung der auf Schlupfverhinderung beruhenden bekmpfenden Wirksamkeit gegenber Anobium punctatum (De Geer) This European Standard was approved by CEN on1993-03-31. CEN members are bound to comply with the CEN/CENEL
15、EC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the Central Secretariat or to any CE
16、N member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official versions. CEN m
17、embers are the national standards bodies of Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and UnitedKingdom. CEN European Committee for Standardization Comit Europen de Normalisation Europis
18、ches Komitee fr Normung Central Secretariat: rue de Stassart 36, B-1050 Brussels 1993 Copyright reserved to CEN members Ref.No.EN370:1993EEN370:1993 BSI 12-1999 2 Foreword This European Standard was drawn up by the “Anobium” Expert Group of CEN/TC38 “Durability of wood and wood-based products”, the
19、secretariat of which is held by AFNOR. The method is new and has been developed to assess the efficacy of eradicant formulations based on non-penetrating fluids which act only on emerging adult beetles and not at depth on larvae established in the wood. This European Standard shall be given the stat
20、us ofa national standard, either by publication of anidentical text or by endorsement, at the latest by October1993, and conflicting national standards shall be withdrawn at the latest by October1993. This European Standard has been approved by CENand in accordance with the Common CEN/CENELEC Rules,
21、 the following countries are bound to implement this European Standard: Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom. Contents Page Foreword 2 Introduction 3 1 Scope 3 2
22、Normative references 3 3 Definitions 3 4 Principle 3 5 Test materials and apparatus 3 6 Sampling 4 7 Test specimens 4 8 Procedure 5 9 Validity of test 7 10 Expression of results 7 11 Test report 7 Annex A (informative) Example of a test report 11 Annex B (informative) Culturing for technique of Anob
23、ium punctatum 12 Annex C (informative) Bibliography 13 Figure 1a Number and distribution oftestspecimens taken from three differenttreesofthe same species 8 Figure 1b Origin and cutting of the test specimen 9 Figure 2 Preparation of subspecimens 10 Figure 3 Distribution of holes in subspecimens 10 T
24、able 1 Numbers of larvae 4 Table A.1 Results 11EN370:1993 BSI 12-1999 3 Introduction This European Standard describes a laboratory method of test which gives a basis for assessment of the eradicant efficacy of a wood preservative, in preventing emergence of Anobium punctatum. It determines the letha
25、l effects, of an insecticidal product, deposited by surface application, on beetles attempting to emerge through treated wood surfaces. The method simulates conditions which can appear in practice where a length of timber infested with Anobium punctatum is treated on all the sides from which emergen
26、ce of beetles is possible. This laboratory method provides one criterion by which the value of a product can be assessed. In making this assessment the methods by which the preservative may be applied should be taken into account. It is further recommended that results from this test should be suppl
27、emented by those from other appropriate tests, and above all by comparison with practical experience. When products which are very active at low concentrations are used it is very important to take suitable precautions to isolate and separate, as far as possible, operations involving chemical produc
28、ts, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel. 1 Scope This European Standard specifies a method for the determina
29、tion of the curative action of a wood preservative against infestation by Anobium punctatum (De Geer) when the product is applied as a surface treatment to wood. This method is applicable to any surface applied treatment that is intended to prevent emergence of adult beetles but not intended to kill
30、 larvae in infested timber. NOTE 1This method may be used in conjunction with an ageing procedure, for example EN73. NOTE 2Products intended to kill larvae should be tested by the method described in EN48. 2 Normative references This European Standard incorporates by dated or undated reference, prov
31、isions from other publications. These normative references are cited at the appropriate places in the text and the publications are listed hereafter. For dated references, subsequent amendments to or revisions of any of these publications apply to this European Standard only when incorporated in it
32、by amendment or revision. For undated references the latest edition of the publication referred to applies. ISO835-1:1981, Laboratory glassware Graduated pipettes Part1: General requirements. ISO3696:1987, Water for analytical laboratory use Specification and test methods. 3 Definitions For the purp
33、oses of this standard, the following definitions apply. 3.1 representative sample a sample having its physical or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled 3.2 supplier the sponsor of the test 4 Principle Preservative is applied by
34、 brush or pipette into test specimens of a susceptible timber. After drying the test specimens are cut into two subspecimens and larvae of Anobium punctatum are introduced into the freshly-cut end grain surfaces. After allowing larvae to establish, the untreated faces are sealed and insects are indu
35、ced to pupate and emerge. The numbers of beetles that emerge and the population that remains within the specimens are compared with those in untreated controls. 5 Test materials and apparatus 5.1 Biological material 5.1.1 Anobium punctatum (De Geer) larvae NOTEThe culturing technique, which experien
36、ce has shown to be suitable, is described in Annex B. 5.1.2 Provision of larvae Carefully split or crumble infested small branchwood to extract larvae. Examine them under a binocular miscroscope and destroy any that show injury or mite infestation or that do not respond by movement when touched.EN37
37、0:1993 4 BSI 12-1999 Weigh the larvae and keep those that have a mass between7mg and12mg, and are in perfect condition. Keep them, for between12h and60h, separately from one another in glass receptacles in the culturing chamber(5.3.1). Re-examine them and reject any which do not show movement in res
38、ponse to stimulation with a fine brush. 5.1.3 Choice of larvae Select sets of12 larvae so that the total mass of each set is between100mg and125mg. The numbers of larvae required are shown in Table 1. Table 1 Numbers of larvae NOTEAdditional larvae may be required to replace larvae which do not esta
39、blish in the test subspecimens. 5.2 Products and reagents 5.2.1 Water, complying with grade3 of ISO3696. 5.2.2 Gelatin, for sealing the relevant surfaces of specimens to be treated with solutions in which an organic solvent is the continuous phase. 5.2.3 Paraffin wax, for sealing the relevant surfac
40、es of specimens to be treated with solutions in which water is the continuous phase. NOTEParaffin wax with a setting point of52 C to54 C has been found to be suitable. 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, controlled at(21 1) C, and at relative humidity(80 5)%. 5.3.2 Laborator
41、y work area, well ventilated, where treatment of the test specimens is carried out. CAUTION. It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours. 5.3.3 Testing chamber, ventilated, controlled at(21
42、 1) C and at relative humidity(70 5)%. 5.3.4 Low temperature regime chamber, either: ventilated and controlled to provide a continuous temperature regime with consecutive cycles of12h at(6 1) C and12h at(13 1) C; or: ventilated and controlled at(6 1) C and relative humidity(70 5)%. 5.3.5 Drill, prov
43、ided with bits capable of drilling smooth cylindrical holes of2mm diameter in wood. 5.3.6 Plastics plates, of opaque unplasticized PVC,50mm 30mm 1mm. 5.3.7 Safety equipment and protective clothing, appropriate for the test product and the test solvent, to ensure the safety of the operator. 5.3.8 Pip
44、ette, of type specified in ISO835-1, classB:graduated pipette with no waiting time. Capacity from0,5ml to25ml with an accuracy of 0,01ml. 5.3.9 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of0,01g. 6 Sampling The sample of preservative shall be representative
45、 of the product to be tested. Samples shall be stored and handled in accordance with any written recommendations from the supplier. NOTEFor the sampling of preservatives from bulk supplies, the procedure given in EN212 should be used. 7 Test specimens 7.1 Species of wood The test shall be carried ou
46、t on Pinus sylvestris (Linnaeus) European redwood, Scots pine. NOTEAdditional tests may be made with other species such as beech (Fagus sylvatica) (Linnaeus) but, if so, this should be stated in the test report. 7.2 Quality of wood Use only sound sapwood, straight-grained and without knots and bark.
47、 The wood shall have an average growth of between two annual growth rings per10mm and eight annual growth rings per10mm (two annual growth rings per10mm to six annual rings per10mm for beech). NOTE 1It is recommended to use test specimens of similar growth rate within a single test. Only sapwood wit
48、h a low resin content shall be used. The proportion of summer wood in the annual rings shall not exceed30% of the whole. The wood shall have been neither floated nor subjected to chemical or heat treatment. It shall be air dried and shall not have been stored for more than five years. NOTE 2Gentle a
49、rtificial drying at below60 C may be used. Number of formulations to be tested Number of test specimens (100mm 50mm 30mm) required Total number of larvae required Untreated controls Treated specimens 1 2 3 4 3 8 6 6 3 6 9 12 144 216 360 432EN370:1993 BSI 12-1999 5 7.3 Provision of test specimens Select the test specimens (which are subsequently cut into two subspecimens) for each test from three trees. For each test the test specimens from each tree shall all be selected from within a1m length of the tree m
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