1、BRITISH STANDARD BS EN 14583:2004 Workplace atmospheres Volumetric bioaerosol sampling devices Requirements and test methods The European Standard EN 14583:2004 has the status of a British Standard ICS 13.040.30 BS EN 14583:2004 This British Standard was published under the authority of the Standard
2、s Policy and Strategy Committee on 22 September 2004 BSI 22 September 2004 ISBN 0 580 44499 6 National foreword This British Standard is the official English language version of EN 14583:2004. The UK participation in its preparation was entrusted by Technical Committee EH/2, Air quality, to Subcommi
3、ttee EH/2/2, Workplace atmospheres, which has the responsibility to: A list of organizations represented on this subcommittee can be obtained on request to its secretary. Cross-references The British Standards which implement international or European publications referred to in this document may be
4、 found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are
5、responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and k
6、eep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document comprises a front cover, an inside front cover, the EN title page, pages 2 to 15 and a back cover. The BSI copyright notice displayed in this document
7、indicates when the document was last issued. Amendments issued since publication Amd. No. Date CommentsEUROPEANSTANDARD NORMEEUROPENNE EUROPISCHENORM EN14583 September2004 ICS13.040.30 Englishversion WorkplaceatmospheresVolumetricbioaerosolsampling devicesRequirementsandtestmethods Airdeslieuxdetrav
8、ailAppareilsdchantillonnage volumtriquedesbioarosolsExigencesetmthodes dessai ArbeitsplatzatmosphreVolumetrische ProbenahmeeinrichtungenfrBioaerosoleAnforderungen undPrfverfahren ThisEuropeanStandardwasapprovedbyCENon9July2004. CENmembersareboundtocomplywiththeCEN/CENELECInternalRegulationswhichstip
9、ulatetheconditionsforgivingthisEurope an Standardthestatusofanationalstandardwithoutanyalteration.Uptodatelistsandbibliographicalreferencesconcernings uchnational standardsmaybeobtainedonapplicationtotheCentralSecretariatortoanyCENmember. ThisEuropeanStandardexistsinthreeofficialversions(English,Fre
10、nch,German).Aversioninanyotherlanguagemadebytra nslation undertheresponsibilityofaCENmemberintoitsownlanguageandnotifiedtotheCentralSecretariathasthesamestatusast heofficial versions. CENmembersarethenationalstandardsbodiesofAustria,Belgium,Cyprus,CzechRepublic,Denmark,Estonia,Finland,France, German
11、y,Greece,Hungary,Iceland,Ireland,Italy,Latvia,Lithuania,Luxembourg,Malta,Netherlands,Norway,Poland,Portugal, Slovakia, Slovenia,Spain,Sweden,SwitzerlandandUnitedKingdom. EUROPEANCOMMITTEEFORSTANDARDIZATION COMITEUROPENDENORMALISATION EUROPISCHESKOMITEEFRNORMUNG ManagementCentre:ruedeStassart,36B1050
12、Brussels 2004CEN Allrightsofexploitationinanyformandbyanymeansreserved worldwideforCENnationalMembers. Ref.No.EN14583:2004:EEN 14583:2004 (E) 2 Contents page Foreword3 Introduction .4 1 Scope 5 2 Normative references 5 3 Terms and definitions .5 4 Abbreviated terms .6 5 Requirements.7 6 Test conditi
13、ons 9 7 Environmental conditions for test methods .11 8 Test report 11 Annex A (informative) Bioaerosol sampling12 Annex B (informative) Example of test facility usable for assessing biological performance of bioaerosol sampling devices13 Bibliography14 EN 14583:2004 (E) 3 Foreword This document (EN
14、 14583:2004) has been prepared by Technical Committee CEN/TC 137 “Assessment of workplace exposure to chemical and biological agents”, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by en
15、dorsement, at the latest by March 2005, and conflicting national standards shall be withdrawn at the latest by March 2005. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgiu
16、m, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EN 14583:2004 (E) 4 Introduction A European
17、 Standard is needed to promote the development of new equipment for measurement of micro- organisms in the work environment. This document can also apply to existing equipment. It is intended to specify requirements and methods to determine performance characteristics of sampling devices used to col
18、lect bioaerosols from the workplace atmosphere. Examples of test environments and methods will be described and test methods will be provided. WARNING The use of this European Standard can involve hazardous materials, operations and equipment. This European Standard does not purport to address all o
19、f the safety problems associated with its use. It is the responsibility of the user of this European Standard to establish appropriate safety and health practices and to determine the applicability of regulatory limitations prior to use. EN 14583:2004 (E) 5 1 Scope This document specifies requiremen
20、ts and test methods to determine the performance of volumetric sampling devices used to assess bioaerosols in the workplace. For clean room measurements EN ISO 14698-1 is applicable. 2 Normative references The following referenced documents are indispensable for the application of this document. For
21、 dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN 1232, Workplace atmospheres Pumps for personal sampling of chemical agents Requirements and test methods EN 12919, Workplace atmospheres Pum
22、ps for the sampling of chemical agents with a volume flow rate of over 5 l/min Requirements and test methods EN 13205, Workplace atmosphere Assessment of performance of instruments for measurement of airborne particle concentrations EN 50015, Electrical apparatus for potentially explosive atmosphere
23、s Oil immersion o EN 50016, Electrical apparatus for potentially explosive atmospheres Pressurised apparatus p. EN 50017, Electrical apparatus for potentially explosive atmospheres Powder filling q. EN 50020, Electrical apparatus for potentially explosive atmospheres Intrinsic safety i. EN 60079-0,
24、Electrical apparatus for potentially explosive atmospheres Part 0: General requirements (IEC 60079-0: 2004) EN 60079-1, Electrical apparatus for potentially explosive atmospheres Part 1: Flameproof enclosure d (IEC 60079-1:2003) EN 60079-7, Electrical apparatusfor explosive gas atmospheres Part 7: I
25、ncreased safety e(IEC 60079-7: 2001) EN 60079-18, Electrical apparatus for explosive gas atmospheres Part 18: Construction, test and marking of type of protection encapsulation “m” electrical apparatus (IEC 60079-18:2004) EN 60079-25, Electrical apparatus for explosive gas atmospheres Part 25: Intri
26、nsically safesystems (IEC 60079-25:2003) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 accuracy closeness of agreement between a test result and the accepted reference value EN 14583:2004 (E) 6 ISO 3534-1:1993 NOTE The quantity referred to
27、in this document as accuracy provides an estimation of the range around the measured value in which can be found the accepted reference value with the confidence of 95 %. 3.2 bias consistent deviation of the results of a measurement process from the true value of the air quality characteristic itsel
28、f EN 482:1994 3.3 culturable number number of micro-organisms, single cells or aggregates able to form colonies on a solid nutrient medium EN 13098:2000 3.4 total number of micro-organisms number of micro-organisms determined as single organisms (or a corresponding measure) EN 13098:2000 3.5 persona
29、l sampler device, which samples air in the breathing zone of a person to determine exposure to biological agents NOTE 1 Some sampling devices have integral pumps, and some do not. Where the instrument requires the use of an external pump, the pump is not subject to the requirements of this document.
30、 NOTE 2 Adapted from EN 1540. 3.6 particle aerodynamic diameter diameter of a sphere of density 1 g cm -3with the same terminal velocity due to gravitational force in calm air, as the particle, under the prevailing conditions of temperature, pressure and relative humidity EN 1540:1998 3.7 sampling d
31、evice total equipment used for sampling, e. g. pump, sampling head and sampling substrate 4 Abbreviated terms ATCC American Type Culture Collection BTC bioaerosol test chamber CBS Centraalbureau voor Schimmelcultures CCUG Culture Collection University of Gteborg DSMZ Deutsche Stammsammlung fr Mikroo
32、rganismen und Zellkulturen HEPA high efficiency particulate aerosol EN 14583:2004 (E) 7 NCTC National Collection of Type Cultures RH relative humidity T temperature 5 Requirements 5.1 General Performance requirements for volumetric sampling pumps shall comply with EN 1232 for low flow rate pumps or
33、EN 12919 for high flow rate pumps. This shall apply both to integral and separate pumps. 5.2 Use in potentially explosive atmospheres When the sampling device covered by this document is to be used in potentially explosive atmospheres, it shall comply with EN 50015 to EN 50017, EN 50020, EN 60079-0,
34、 EN 60079-1, EN 60079-7, EN 60079-18 and EN 60079-25. 5.3 Mechanical construction Every sampling device shall be constructed in such a manner that it is easily accessible for regular function checks and that airflow can easily be measured and calibrated. The sampling pump shall maintain the required
35、 airflow rate throughout the sampling period. NOTE Material used in the sampling head should be chosen to avoid moisture uptake and electrostatic charges. 5.4 Indicator devices An indicator device shall be provided to show that the sampling device is switched on. If the sampling device has more than
36、 one measuring range, the selected range shall be clearly identified. NOTE It is an advantage if elapsed time indicators, low flow rate indicators, flow interrupted indicators are given. 5.5 Adjustments Any equipment (switch, knob, etc.) used for modifying the operating parameters (sampling time, fl
37、ow rate, etc) of the sampling device shall be protected against involuntary action during sampling. The operational settings should be displayed. 5.6 Battery powered sampling devices Sampling devices powered with integral batteries shall be provided with an early indication of low battery condition.
38、 Short recharge time, light-weight and low noise levels are recommended. 5.7 Airflow-control During the sampling deviation from the required airflow should not exceed 5 %. 5.8 Airflow-meter A device to measure the airflow through the sampling device before and after sampling in the field should be s
39、upplied if required. NOTE This device can be different from that used for calibration. EN 14583:2004 (E) 8 Sampling devices with integral airflow meters shall be calibrated against a traceable external airflow meter before use. 5.9 Labelling and marking The source and the manufacturer of the samplin
40、g device shall be clearly identified to ensure traceability to published performance characteristics. 5.10 Instruction manual The instruction manual shall be written in a language, which is understandable in the country of the operator. It shall be easily understood and every function explained. It
41、shall illustrate all operation knobs and their handling by figures. It shall give the environmental and other conditions under which the instrument shall be operated including limitations to its use. It shall give exact calibration instructions and recommended equipment (e.g. flow meters) to be used
42、. The manual shall also give the address for service of the instrument. 5.11 Fraction to be sampled To measure personal exposure to bioaerosols the sampling device should follow the criteria for size fractions of airborne particles according to EN 481. 5.12 Physical sampling efficiency Physical samp
43、ling efficiency is defined in EN 13098 and EN 13205. It shall be measured as a function of particle aerodynamic diameter and other influent parameters. NOTE 1 Annex A gives some information about the physical behaviour of a sampling device and the experimental assessment of its physical sampling eff
44、iciency in the laboratory. Physical sampling efficiency can be interpreted as the percentage of ambient particles of specific size that are sampled and collected by the sampling device. As airborne micro-organisms often adhere to non-biological particles of various sizes, the measurement of micro-or
45、ganism concentration implies an accurate knowledge of this parameter. Sampling devices shall collect a representative sample of the required health related fraction of the bioaerosol (see EN 481). When assessing the performance of any bioaerosol sampling device, the overall sampling efficiency shall
46、 be determined. The performance of a personal sampler will be affected by the proximity of the operators body when worn within the breathing zone, and therefore personal inhalable sampling devices need to be assessed whilst attached to a torso or other well characterised equivalents. NOTE 2 Personal
47、 sampling is performed when assessing the exposure to evaluate a suspected disease. Many biological tests are more effectively carried out with a personal sampler used as a free standing device. A free-standing sampler can be used to scan the environment for high emissions of micro-organisms. 5.13 P
48、reservation efficiency Preservation efficiency is defined in EN 13098 as the capacity of the sampling device to maintain the culturability of the airborne micro-organisms during collection and also to keep the microbial products intact. The loss of culturability of micro-organisms due to sampling stress shall be tested on relevant model organisms. EN 14583:2004 (E) 9 5.14 Concentration range The manufacturer shall give the operational range and its limitations. An ideal sampling device should be able to sample conce
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