BS EN 16086-1-2011 Soil improvers and growing media Determination of plant response Pot growth test with Chinese cabbage《土壤改进剂和生长剂 植物反应测定 用大白菜进行盆栽试验》.pdf

1、BS EN 16086-1:2011Soil improvers and growingmedia Determination ofplant responsePart 1: Pot growth test with ChinesecabbageBS EN 16086-1:2011 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of EN 16086-1:2011.BSI, as a member of CEN, is obliged to publish EN 16086-1:2

2、011 as a British Standard. However, attention is drawn to the fact the UK committee voted against its approval as a European Standard.The UK committee voted against the publication of this standard because the plant species specified for the test are not sensitive to some of the pesticides of greate

3、st concern currently in composted materials, and because it considered that the reproducibility ob-tained in inter-laboratory evaluation was not an adequate basis on which to establish a reference method. This could cause problems in the event of dispute or litigation.The standard deviations of repr

4、oducibility in the inter-laboratory validation trials of this method were poor and there is a low probability of getting the same result from two laboratories analysing the same sample. In the worst cases the standard deviation of reproducibility was about half the mean analysis (after re-jection of

5、 outliers); for example the mean weight of plants per pot in compost 1 was 12.36 g, SR6.95.The UK committee advises that this standard will not detect inhibition by residues of some of the herbicides of greatest concern, and that in addition the results are imprecise.The UK participation in its prep

6、aration was entrusted to Technical Committee AW/20, Top soil and other growing media.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for it

7、s correct application. BSI 2011ISBN 978 0 580 70565 6ICS 65.080Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 November 2011.Amendments/corrigenda issued sinc

8、e publicationDate T e x t a f f e c t e dEUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 16086-1 November 2011 ICS 65.080 English Version Soil improvers and growing media - Determination of plant response - Part 1: Pot growth test with Chinese cabbage Amendements du sol et supports de culture -

9、 Dtermination de la rponse des plantes - Partie 1: Essai de croissance en pot avec du chou de Chine Bodenverbesserungsmittel und Kultursubstrate - Bestimmung der Pflanzenvertrglichkeit - Teil 1: Wachstumstest mit Chinakohl im Topf This European Standard was approved by CEN on 17 September 2011. CEN

10、members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on appli

11、cation to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Man

12、agement Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Nethe

13、rlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2011 CEN All rights of exploitation

14、in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16086-1:2011: E2 Contents Page Foreword 41 Scope 52 Normative references 53 Terms and definitions .54 Principle of the plant growth response test 64.1 General 64.2 Pot experiment with direct use of the prepared sam

15、ple 64.3 Pot experiment using an extract of the original sample 65 Choice of methodology .66 Pot experiment with direct use of the prepared sample 66.1 Materials .66.1.1 Water of class 366.1.2 Sphagnum peat 66.1.3 Fertilized and limed sphagnum peat66.1.4 Ground limestone 76.1.5 Seeds of Chinese cabb

16、age (Brassica napa, ssp. pekinensis) 76.1.6 Seeds of Spring barley (Hordeum vulgare) .76.2 Apparatus .76.3 Preparation of the sample .76.3.1 General preparation .76.3.2 Mixing procedure .86.4 Test procedure 106.5 Control sample 106.6 Validity of the test . 106.7 Evaluation parameters . 116.7.1 Germi

17、nation rate (%) 116.7.2 Fresh weight 126.7.3 Growth inhibition 136.7.4 Abnormalities in comparison to the control 137 Pot experiment using an extract of the original sample . 147.1 Materials 147.1.1 Water of class 3. 147.1.2 Perlite . 147.1.3 Seeds of Chinese cabbage (Brassica napa, ssp. pekinensis)

18、 . 147.1.4 Seeds of Spring barley (Hordeum vulgare) 147.1.5 Balanced nutrient solution (see B.1) 147.1.6 Nitric acid (HNO3), 1M . 147.2 Apparatus 147.2.1 Sieve with 20 mm mesh size 147.2.2 Circular plant pot 147.2.3 Saucer 147.2.4 Bucket 147.2.5 Thin fleece or plastic sheet for covering the containe

19、rs 157.2.6 Testing facility . 157.2.7 Irrigation device for watering the pots . 157.2.8 Analytical balance. 157.3 Preparation of the sample 157.3.1 General preparation 15BS EN 16086-1:2011 EN 16086-1:2011 (E)3 7.3.2 Extraction procedure 157.4 Test procedure . 157.5 Control sample 167.6 Validity of t

20、he test 167.7 Evaluation parameters 168 Precision. 169 Test report 16Annex A (informative) Validation . 18Annex B (normative) Nutrient supply and fist test 21B.1 Composition of the nutrient solution 21B.2 Possible adjustments of the nutrient supply during the test . 22B.2.1 Possible reasons for nutr

21、itional adjustments 22B.2.2 Recommendations for nutritional adjustments . 22B.2.3 Supplement to the report 23B.3 Fist test . 24Bibliography 25BS EN 16086-1:2011 EN 16086-1:2011 (E)4 Foreword This document (EN 16086-1:2011) has been prepared by Technical Committee CEN/TC 223 “Soil improvers and growi

22、ng media”, the secretariat of which is held by ASI. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by May 2012, and conflicting national standards shall be withdrawn at the latest by May 2012. Atten

23、tion is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. SAFETY PRECAUTIONS Care should be taken when handling samples that may contain sharps or is

24、 of a dusty nature. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hunga

25、ry, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. BS EN 16086-1:2011 EN 16086-1:2011 (E)5 1 Scope This European Standard describes a method for the routine determin

26、ation of the effect of soil improvers and growing media or constituents thereof on the growth of Chinese cabbage (and in certain cases spring barley). This test may not be suitable for all growing media since the growing media characteristics (e.g. nutrient content) will vary according to target use

27、 and the product is not tested in accordance with the specified use and pack recommendations. This test is not appropriate for the detection of nitrogen immobilization. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated referen

28、ces, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN 13037, Soil improvers and growing media Determination of pH EN 13038, Soil improvers and growing media Determination of electrical conductivity EN 13040,

29、Soil improvers and growing media Sample preparation for chemical and physical tests, determination of dry matter content, moisture content and laboratory compacted bulk density EN ISO 3696, Water for analytical laboratory use Specification and test methods (ISO 3696:1987) 3 Terms and definitions For

30、 the purposes of this document, the following terms and definitions apply. 3.1 plant response variation in plant germination and/or growth when sown and grown in a growing medium, soil improver or constituent thereof or in an extract obtained from these materials Factors causing negative plant growt

31、h cannot be identified nor quantified by applying this method. 3.2 prepared sample portion of the laboratory sample, undiluted or diluted with sphagnum peat at given ratios, fertilized and limed as required BS EN 16086-1:2011 EN 16086-1:2011 (E)6 4 Principle of the plant growth response test 4.1 Gen

32、eral Depending on the material to be tested, one of the two methods described in this standard shall be used. 4.2 Pot experiment with direct use of the prepared sample Sowing a defined quantity of Chinese cabbage into pots containing the prepared sample, cultivating under controlled conditions for a

33、 defined period of time and evaluating the plant response by determining the germination rate, fresh weight, abnormalities and overall plant growth. If the presence of graminacious herbicides is suspected, Spring barley shall be used in addition to Chinese cabbage. For testing of other specific effe

34、cts, the use of additional plant species (for example lettuce) can be considered. 4.3 Pot experiment using an extract of the original sample Mixing the original sample with nutrient solution as an extractant, soaking for 4 h at ambient temperature and collecting the freely available nutrient solutio

35、n. Filling pots with perlite saturated with the extract and continuing as described under 4.2, irrigating during the test period with a fixed quantity of the extract and afterwards water. If the presence of graminacious herbicides is suspected, Spring barley shall be used in addition to Chinese cabb

36、age. For testing of other specific effects, the use of additional plant species (for example lettuce) can be considered. 5 Choice of methodology For most materials, the pot growth test can be carried out as described in Clause 6. However, for coarse materials such as bark, expanded clay, lava, miner

37、al wool, perlite, polyurethane and pumice with an inherently low water holding capacity which are used as growing media without amendment, this procedure is unsuitable and the extract method described in Clause 7 shall be adopted. 6 Pot experiment with direct use of the prepared sample 6.1 Materials

38、 6.1.1 Water of class 3 According to EN ISO 3696. 6.1.2 Sphagnum peat Sphagnum peat with a degree of humification of H3 H5 according to von Post scale, having a pH measured according to EN 13037 of between 3,0 and 4,5, an EC measured according to EN 13038 of between 1 mS m-1and 5 mS m-1, a particle

39、size of 10 mm and to which neither lime nor fertilizer has been added. 6.1.3 Fertilized and limed sphagnum peat Sphagnum peat (see 6.1.2), having a pH measured according to EN 13037 adjusted using ground limestone (see 6.1.4) to a range between 5,5 and 6,5, fertilized with a water soluble “complete”

40、 fertilizer with essential BS EN 16086-1:2011 EN 16086-1:2011 (E)7 micronutrients, added at a rate to supply (225 25) mg N l-1, (for example 1,5 g l-1water soluble complete fertilizer N : P2O5 : K2O 15 : 10 : 20), (see B.1). 6.1.4 Ground limestone Finely ground limestone, containing at least 5 % MgC

41、O3, having a particle size less than 1 mm and a moisture content of less than 1 % m/m. 6.1.5 Seeds of Chinese cabbage (Brassica napa, ssp. pekinensis) Specified germination capacity 95 %. 6.1.6 Seeds of Spring barley (Hordeum vulgare) Specified germination capacity 95 %. 6.2 Apparatus 6.2.1 Sieve wi

42、th 20 mm mesh size 6.2.2 Sieve with 5 mm mesh size 6.2.3 Circular plant pot Upper diameter (12 0,5) cm , height between 8,5 cm and 9,0 cm, volume between 650 ml and 700 ml, perforated bottom to provide drainage (for example plastic pot used in horticulture). 6.2.4 Saucer Saucer capable of catching a

43、ll surplus water from the plant container after overhead watering. 6.2.5 Thin fleece or plastic sheet for covering the containers 6.2.6 Testing facility Testing facility capable of maintaining and monitoring the temperature and light intensity specified in 6.4 such as a greenhouse or plant growth ro

44、om. 6.2.7 Irrigation device for watering the pots For example watering can, greenhouse watering hose 6.2.8 Analytical balance Accuracy 0,01 g, capacity 500 g. 6.3 Preparation of the sample 6.3.1 General preparation Pass the sample through a 20 mm sieve (see 6.2.1). Any foreign material such as plast

45、ic, metal or glass retained on the sieve shall be removed and noted. Other material that is retained on the sieve and which is an intrinsic part of the sample shall be physically reduced to parts of similar size as few times as are necessary to permit the entire sample to pass through the sieve. Fib

46、rous materials i.e. coir fibres and straw shall be cut to a length 20 mm by using scissors. Thoroughly mix the laboratory sample with the broken particles that had been retained on the sieve taking care to minimise physical damage to the sample as a whole. Transportation BS EN 16086-1:2011 EN 16086-

47、1:2011 (E)8 and possible storage of the samples shall be done in accordance with EN 13040, using food grade polyethylene bags. 6.3.2 Mixing procedure Before mixing, the laboratory compacted bulk density shall be measured (according to EN 13040). To produce the prepared sample, the material prepared

48、as described in 6.3.1 is thoroughly mixed with sphagnum peat (see 6.1.2) on a volume/volume basis as given in Table 1. The pH according to EN 13037 is ideally within the range between 5,5 and 6,5. If it is below, the pH shall be adjusted by adding limestone (see 6.1.4). If it is above, the pH shall

49、be noted. Additionally, a water soluble complete fertilizer with essential micronutrients, added at a rate to supply (225 25) mg N l-1(for example 1,5 g l-1water soluble complete fertilizer N : P2O5 : K2O 15 : 10 : 20) (see B.1) is added to one litre of prepared sample (according to EN 13040). The dilution ratios in Table 1 are based on horticultural practice in the usage of growing media, growing media constitue