1、raising standards worldwideNO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBSI Standards PublicationBS EN 16150:2012Water quality Guidanceon pro-rata Multi-Habitatsampling of benthic macro-invertebrates from wadeableriversBS EN 16150:2012 BRITISH STANDARDNational forewordThis B
2、ritish Standard is the UK implementation of EN 16150:2012.The UK participation in its preparation was entrusted to TechnicalCommittee EH/3/5, Biological Methods.A list of organizations represented on this committee can beobtained on request to its secretary.This publication does not purport to inclu
3、de all the necessaryprovisions of a contract. Users are responsible for its correctapplication. The British Standards Institution 2012. Published by BSI StandardsLimited 2012ISBN 978 0 580 71890 8ICS 13.060.70Compliance with a British Standard cannot confer immunity fromlegal obligations.This Britis
4、h Standard was published under the authority of theStandards Policy and Strategy Committee on 31 May 2012.Amendments issued since publicationDate Text affectedBS EN 16150:2012EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 16150 April 2012 ICS 13.060.70 English Version Water quality - Guidance
5、on pro-rata Multi-Habitat sampling of benthic macro-invertebrates from wadeable rivers Qualit de leau - Lignes directrices pour lchantillonnage des macroinvertbrs benthiques en cours deau peu profonds au prorata des surfaces de recouvrement des habitats prsents Wasserbeschaffenheit - Anleitung fr di
6、e pro-rata Multi-Habitat-Probenahme benthischer Makroinvertebraten in Flssen geringer Tiefe (watbar) This European Standard was approved by CEN on 16 March 2012. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard
7、 the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, F
8、rench, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgar
9、ia, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITT
10、EE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2012 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16150:2012: EBS EN 16150:2012EN 16150:20
11、12 (E) 2 Contents Page Foreword 31 Scope 42 Normative references 43 Terms and definitions .44 Description of the sampling approach 65 Field sampling procedures .75.1 Factors limiting effective Multi-Habitat-Sampling (MHS).75.2 Estimation of habitat composition .75.3 Allocation of sampling units .76
12、Detailed description of sampling procedures 86.1 General recommendations for sampling .86.2 Megalithal (bedrock and boulders) 96.3 Macrolithal and mesolithal (cobbles, stones, pebbles) .96.4 Microlithal and smaller mineral substrates .96.5 Xylal (woody debris) 96.6 Roots 106.7 Coarse Particulate Org
13、anic Matter (CPOM/leaf litter) . 106.8 Macrophytes (emergent and submerged) 106.9 Technolithal . 107 Sample treatment 107.1 Removal of large material and sorting . 107.2 Removal of large organisms 107.3 Storage . 107.4 Labelling 107.5 Refining the site-protocol 11Annex A (informative) Sampling proto
14、cols 12Bibliography . 14BS EN 16150:2012EN 16150:2012 (E) 3 Foreword This document (EN 16150:2012) has been prepared by Technical Committee CEN/TC 230 “Water analysis”, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publica
15、tion of an identical text or by endorsement, at the latest by October 2012, and conflicting national standards shall be withdrawn at the latest by October 2012. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC sh
16、all not be held responsible for identifying any or all such patent rights. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, De
17、nmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. BS EN 16150:2012EN 16150:2012 (E) 4 1 Scope This Eu
18、ropean Standard gives guidance on procedures for the pro-rata Multi-Habitat-Sampling (MHS) of benthic macro-invertebrates in wadeable rivers and streams. The term “pro-rata“ reflects the intention to sample adequate proportions of riverine habitats with reference to their percentage occurrence. The
19、pro-rata MHS technique does not replace other techniques, but is rather, alongside other applications, a fundamental requisite of some multi-metric assessment approaches used to evaluate the ecological status of running waters. The method described in this document is one of the possible techniques
20、among the existing pro-rata MHS techniques. The MHS methodology is based on Rapid Bioassessment Protocols 1, the procedures of the Environment Agency for England and Wales 2, the Austrian Guidelines for the Assessment of the Saprobiological Water Quality of Rivers and Streams 3, the AQEM sampling ma
21、nual 4, the AQEM grain diameter 0,2 cm to 2 cm 3.2 argyllal silt, loam, clay 3.3 debris organic and inorganic matter deposited within the splash zone area by wave-motion and changing water levels 3.4 hygropetric sites thin water layer on solid (rocky) substrates 3.5 investigation site specific area
22、of an investigated river reach for sampling benthic organisms BS EN 16150:2012EN 16150:2012 (E) 5 3.6 macro-algae strands of filamentous algae and algal tufts 3.7 macrolithal coarse blocks, cobbles, gravel and sand; grain diameter 20 cm to 40 cm 3.8 megalithal upper sizes of large cobbles, boulders,
23、 blocks and bedrock; grain diameter 40 cm 3.9 mesolithal fist to hand-sized cobbles with a variable percentage of gravel and sand; grain diameter 6 cm to 20 cm 3.10 micro-algae algal films 3.11 microlithal coarse gravel (size of a pigeon egg to a childs fist) with variable percentages of medium to f
24、ine gravel; grain diameter 2 cm to 6 cm 3.12 pelal mud and sludge; grain diameter 40 cm large cobbles, bolders and blocks, bedrock Macrolithal 20 cm to 40 cm coarse blocks, head-sized cobbles (with variable percentages of cobbles, gravel and sand)Mesolithal 6 cm to 20 cm fist to hand-sized cobbles (
25、with variable percentages of gravel and sand) Microlithal 2 cm to 6 cm coarse gravel (size of a pigeon egg to childs fist) (with variable percentages of medium to fine gravel) Akal 0,2 cm to 2 cm fine to medium-sized gravel Psammal 6 m to 2 mm sand Psammopelal mixture of sand with mud Pelal 40 cm ma
26、crolithal 20 cm to 40 cm mesolithal 6 cm to 20 cm microlithal 2 cm to 6 cm akal 0,2 cm to 2 cm psammal 6 m to 2 mm psammopelal pelal 6 m Argyllal 6 m not allocable % SU % SU % SU % SU % SU % SU % SU % SU % SU % SU % SU bare mineral substrate BIOTICHABITATS- percent coverage(sumvariable)micro-algae m
27、acro-algae submerged macrophytes emergent macrophytes living parts of terrestial plants xyla CPOM FPOM debris sewage, bacteria and fungi 2) The user of this form is allowed to copy this present form. BS EN 16150:2012EN 16150:2012 (E) 14 Bibliography 1 BARBOUR, M.T., GERRITSEN, J., SNYDER, B.D. & STR
28、IBLING, J.B. (1999): Rapid bioassessment protocols for use in streams and wadable rivers: periphyton, benthic macroinvertebrates and fish. (2nd Ed.) EPA 841-B-99-002. U.S. Environmental Protection Agency, Office of Water, Washington, D.C. 2 MURRAY-BLIGH, J.A.D. (1999): Procedures for collecting and
29、analysing macro-invertebrate samples. Quality Management Systems for Environmental Monitoring: Biological Techniques, BT001. (Version 2.0, 30 July 1999) - Environment Agency, Bristol, UK. 3 MOOG, O., CHOVANEC, A., HINTEREGGER, J. & RMER, A. (1999): Austrian Guidelines for the assessment of the sapro
30、biological water quality of rivers and streams (in German: Richtlinie zur Bestimmung der saprobiologischen Gewssergte von Fliegewssern).- Bundesministerium fr Land- und Forstwirtschaft. Wasserwirtschaftskataster, Wien, 144 pp. 4 AQEM consortium (2002): Manual for the application of the AQEM system.
31、A comprehensive method to assess European streams using benthic macroinvertebrates, developed for the purpose of the Water Framework Directive. Version 1.0. (www.aqem.de). 5 AQEM & STAR Site Protocol (2002): www.eu-star.at. Protocols. 6 OENORM M 6119-2 (draft), Guidelines for the ecological study an
32、d assessment of rivers Macrozoobenthos. Part 2: A Standardized Procedure for prorate Multi-Habitat-Sampling. 7 OENORM M 6232, Guidelines for the ecological study and assessment of rivers (bilingual edition) 8 DIN 38410-1, German standard methods for the examination of water, waste water and sludge B
33、iological-ecological analysis of water (group M) Part 1: Determination of the saprobic index in running waters (M 1) 9 XP T90-333, Water quality Sampling of aquatic macro-invertebrates in shallow freshwaters This page deliberately left blankBSI is the independent national body responsible for prepar
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