BS EN 60068-2-10-2005 Environmental testing - Tests - Test J and guidance Mould growth《环境试验 试验 试验J和指南 长霉》.pdf

1、 g49g50g3g38g50g51g60g44g49g42g3g58g44g55g43g50g56g55g3g37g54g44g3g51g40g53g48g44g54g54g44g50g49g3g40g59g38g40g51g55g3g36g54g3g51g40g53g48g44g55g55g40g39g3g37g60g3g38g50g51g60g53g44g42g43g55g3g47g36g58Test J and guidance: Mould growth The European Standard EN 60068-2-10:2005 has the status of a Brit

2、ish StandardICS 19.040Environmental testingPart 2-10: Tests BRITISH STANDARDBS EN 60068-2-10:2005BS EN 60068-2-10:2005This British Standard was published under the authority of the Standards Policy and Strategy Committee on 9 November 2005 BSI 9 November 2005ISBN 0 580 46585 3A list of organizations

3、 represented on this committee can be obtained on request to its secretary.Cross-referencesThe British Standards which implement international or European publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence In

4、dex”, or by using the “Search” facility of the BSI Electronic Catalogue or of British Standards Online.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application.Compliance with a British Standard does not of itself conf

5、er immunity from legal obligations.Summary of pagesThis document comprises a front cover, an inside front cover, the EN title page, pages 2 to 33 and a back cover.The BSI copyright notice displayed in this document indicates when the document was last issued.Amendments issued since publicationAmd. N

6、o. Date Comments aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep UK interests informed; monitor related international and European developments and promulgate them in the UK.Natio

7、nal forewordThis British Standard is the official English language version of EN 60068-2-10:2005. It is identical with IEC 60068-2-10:2005. It supersedes BS 2011-2.1J:1989 which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee GEL/104, Environmental condition

8、s, classification and testing, which has the responsibility to:EUROPEAN STANDARD EN 60068-2-10 NORME EUROPENNE EUROPISCHE NORM July 2005 CENELEC European Committee for Electrotechnical Standardization Comit Europen de Normalisation Electrotechnique Europisches Komitee fr Elektrotechnische Normung Ce

9、ntral Secretariat: rue de Stassart 35, B - 1050 Brussels 2005 CENELEC - All rights of exploitation in any form and by any means reserved worldwide for CENELEC members. Ref. No. EN 60068-2-10:2005 E ICS 19.040 Supersedes HD 323.2.10 S3:1988English version Environmental testing Part 2-10: Tests Test J

10、 and guidance: Mould growth (IEC 60068-2-10:2005) Essais denvironnement Partie 2-10: Essais Essai J et guide: Moisissures (CEI 60068-2-10:2005) Umgebungseinflsse Teil 2-10: Prfverfahren Prfung J und Leitfaden: Schimmelwachstum (IEC 60068-2-10:2005) This European Standard was approved by CENELEC on 2

11、005-06-01. CENELEC members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may b

12、e obtained on application to the Central Secretariat or to any CENELEC member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CENELEC member into its own language and notified to th

13、e Central Secretariat has the same status as the official versions. CENELEC members are the national electrotechnical committees of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Ne

14、therlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EN 60068-2-10:2005 2 Foreword The text of document 104/365/FDIS, future edition 6 of IEC 60068-2-10, prepared by IEC TC 104, Environmental conditions, classification and methods of test, was submi

15、tted to the IEC-CENELEC parallel vote and was approved by CENELEC as EN 60068-2-10 on 2005-06-01. This European Standard supersedes HD 323.2.10 S3:1988. The main changes with respect to HD 323.2.10 S3:1988 are: two test fungi replaced by two others; concentration of the spores defined for each test

16、fungus; spores suspension in mineral salt solution additionally introduced; pre-conditioning of the specimens by damp heat storage prescribed; supersonic aerosolization of the spores suspension as the preferred inoculation method introduced; duration of incubation reduced from 84 days to 56 days; ex

17、tent of mould growth grade 2 split into grade 2a and grade 2b; detailed information on methods of inoculation given in Annex B; Annex E: flow-chart deleted. The following dates were fixed: latest date by which the EN has to be implemented at national level by publication of an identical national sta

18、ndard or by endorsement (dop) 2006-03-01 latest date by which the national standards conflicting with the EN have to be withdrawn (dow) 2008-06-01 Annex ZA has been added by CENELEC. _ Endorsement notice The text of the International Standard IEC 60068-2-10:2005 was approved by CENELEC as a European

19、 Standard without any modification. _ 3 EN 60068-2-10:2005 CONTENTS 1 Scope 5 2 Normative references .5 3 General description 5 4 Health hazards to operators6 5 Description of the test variants6 5.1 Test variant 1 6 5.2 Test variant 2 6 6 Reagents and materials 7 6.1 Cultures or spores Supply and co

20、nditions7 6.2 Preparation of spore suspension8 6.2.1 General .8 6.2.2 Preparation for test variant 1 8 6.2.3 Preparation for test variant 2 9 6.3 Control strips.9 7 Description of test apparatus 9 7.1 Inoculation by spraying 9 7.2 Incubation of small specimens .9 7.3 Incubation of large specimens10

21、8 Severities.10 9 Initial examinations.10 10 Pre-conditioning .10 10.1 Cleaning10 10.2 Damp heat storage 11 11 Conditioning .11 11.1 Application 11 11.1.1 Test variant 1.11 11.1.2 Test variant 2.11 11.2 Inoculation 11 11.3 Incubation .12 12 Final examinations12 12.1 Visual examination 12 12.2 Effect

22、 of growth .13 12.3 Extent of growth 13 13 Information to be given in the relevant specification.14 14 Information to be given in the test report as a minimum .14 Annex A (informative) Danger to personnel 15 A.1 General .15 A.2 Notes for the guidance of medical officers15 Annex B (normative) Inocula

23、tion methods (see also 11.2)17 B.1 General .17 B.2 Spraying by the aerosol inoculation method (AIM) 17 B.2.1 General .17 B.2.2 Description of the method 17 B.3 Inoculation of small specimens by dipping19 B.4 Inoculation of large specimens by spraying or painting .19 EN 60068-2-10:2005 4 Annex C (inf

24、ormative) Recommended safety precautions20 Annex D (informative) Decontamination procedures22 D.1 Recommended decontamination procedures 22 D.1.1 Application of chemical active solutions22 D.1.2 Autoclaving22 D.1.3 Wiping or spraying with ethanol 70 % vol22 D.1.4 Application of volatile disinfectant

25、s.23 D.2 Disposal 23 Annex E (informative) Information on the test fungi.24 E.1 List of identical strains .24 E.2 Recommended nutritive media for maintaining the cultures.25 Annex F (informative) Guidance .26 F.1 Mechanisms of infection 26 F.2 Germination and growth.26 F.3 Effects of growth .27 F.3.

26、1 Primary effects.27 F.3.2 Secondary effects 27 F.3.3 Effects associated with equipment design.27 F.4 Prevention of mould growth28 F.5 Applicability of the mould growth test .29 F.6 Basic types of effect 30 F.6.1 Extent of growth and attack on the surface after 28 days of incubation30 F.6.2 Effect o

27、n performance while still damp after 28 days incubation (variant 2) or after 28 or 56 days of incubation (variant 1) .30 F.6.3 Effect on performance after a recovery period of 24 h.31 F.7 Use of fungicides.31 F.7.1 Limitation of use 31 F.7.2 Performance of fungicidal action.32 F.7.3 Duration of prot

28、ection and testing.32 5 EN 60068-2-10:2005 ENVIRONMENTAL TESTING Part 2-10: Tests Test J and guidance: Mould growth 1 Scope This part of IEC 60068 provides a test method for determining the extent to which electrotechnical products support mould growth and how any mould growth may affect the perform

29、ance and other relevant properties of the product. Since mould growth conditions include high relative humidity, the test is applicable to electrotechnical products intended for transportation, storage and use under humid conditions over a period of some days at least. 2 Normative references The fol

30、lowing referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/IEC 17025:1999, General requirements for the compete

31、nce of testing and calibration laboratories ISO 846:1997, Plastics Evaluation of the action of microorganisms MILSTD810 F:2000, Method 508.5 Fungus Laboratory Biosafety Manual 2ndEd., WHO 1993, ISBN 92 4 1544503 3 General description This test covers the inoculation of electrotechnical products with

32、 a selection of mould spores followed by a period of incubation under conditions which promote spore germination and the growth of mould. Two variations of the test are given. Variant 1 specifies inoculation of the specimen with the mould spores without nutrients whereas variant 2 specifies the inoc

33、ulation with the mould spores suspended in a nutritive solution which supports mould growth. It is advisable to use testing procedures such as specified for plastics in ISO 846 to assess the vulnerability to damage by mould growth of the constructional materials used. NOTE Laboratories for microbiol

34、ogical testing of technical products should be accredited in accordance with ISO/IEC 17025. See further Annex F. EN 60068-2-10:2005 6 Surface contamination in the form of dusts, splashes, condensed volatile nutrients or grease may be deposited upon assembled specimens. This can be brought about by s

35、torage and use or transport with the product exposed to the atmosphere or handled without protective covering. This surface contamination can cause an increased colonization by fungi and may lead to greater growth and damage. An assessment of the effect of such contamination can be given by the appl

36、ication of test variant 2. Due to the difficulty of maintaining the necessary conditions in a very large chamber, a large composite equipment will normally be tested as a number of sub-units. This will in any case minimize the cost of the test since several sub-units may be so similar in constructio

37、n that only one of them need be tested. 4 Health hazards to operators This test procedure requires the use of viable mould spores and the application of ambient conditions which promote mould growth. Therefore before any attempt is made to handle mould cultures, or to carry out steps of the test sub

38、sequently described, it is important that the annexes of this standard be studied. Annex A Danger to personnel Annex B Inoculation methods Annex C Recommended safety precautions Annex D Decontamination procedures Laboratory Biosafety Manual, 2ndEd., World Health Organization 1993, ISBN 92 4 1544503

39、includes general background reading on safety in facilities dealing with fungi. 5 Description of the test variants 5.1 Test variant 1 After a 28 days incubation period determine the extent of mould growth by visual inspection; the physical damage caused by mould growth; in the case of mould growth t

40、he effect on functioning and/or electrical properties if required in the relevant specification. The incubation period shall be extended to a total of 56 days before checking the function and/or measuring electrical properties if required in the relevant specification. 5.2 Test variant 2 After a sim

41、ulated contamination with nutrients followed by a 28 days incubation period determine the extent of mould growth by visual inspection; the physical damage caused by mould growth; the effect of the mould growth on functioning and/or electrical properties if required in the relevant specification. 7 E

42、N 60068-2-10:2005 The surface resistance of the specimen will be reduced by application of nutrients for simulation of contamination without any mould growth. This effect should be considered if checking the function and/or measuring electrical properties. Due to the application of nutrients, mould

43、growth will exist; failing this, a fungicidal effect shall be considered. 6 Reagents and materials 6.1 Cultures or spores Supply and conditions The following fungi shall be used for performing the test (see Table 1). The nature of the attack to be expected from each fungus is indicated for guidance.

44、 The spores of all cultures, however, shall be used together in a mixed suspension whatever the nature of the specimen. The cultures or freeze-dried spores shall be obtained from a recognized mycological cultures collection. They shall be supplied in containers with the date of inoculation of the cu

45、lture thereon. A certificate shall confirm the accordance of the culture with the fungus and strain number as specified in Table 1 and/or Annex E. Cultures and freeze-dried spores shall be handled and stored in accordance with the recommendations of the supplier and the relevant requirements of this

46、 standard. Preparing a culture by the test laboratory from a stock culture or from freeze-dried spores the date of inoculation shall be marked on the culture tube. Table 1 Test fungi No. Name Strain No. 3)Attacks Note 1 Aspergillus niger ATCC 6275 many materials 1) 2) 2 Aspergillus terreus ATCC 1069

47、0 plastic materials 1) 2) 3 Chaetomium globosum ATCC 6205 cellulose 1) 2) 4 Hormoconis resinae DSM 1203 hydrocarbon based lubricants 5 Paecilomyces variotii ATCC 18502 plastics and leather 1) 2) 6 Penicillium funiculosum ATCC 36839 many materials especially textiles 1) 2) 7 Scopulariopsis brevicauli

48、s ATCC 36840 rubber 1) 2) 8 Trichoderma virens ATCC 9645 cellulose, textiles and plastics 2) 1) 2) 3) Also specified in ISO 846. Also specified in MILSTD810 F, Table 508.5. See also Annex E. EN 60068-2-10:2005 8 Cultures shall be used for preparing the test spore suspension when they are well sporul

49、ated. This is reached in most cases after a 7 to 14 days incubation period at (29 1) C. NOTE The supplier of cultures or freeze-dried spores may recommend other conditions to develop the culture. If the cultures are not for immediate use, they shall be stored in a refrigerator at a temperature between 5 C and 10 C, for a continuous period of not more than six weeks commencing not earlier than 14 days and not later than 28 days