BS EN ISO 5667-3-2012 en 4998 Water quality Sampling Preservation and handling of water samples《水质 采样 水样的保存和处理》.pdf

1、raising standards worldwideNO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBSI Standards PublicationBS EN ISO 5667-3:2012Water quality SamplingPart 3: Preservation and handling of watersamples (ISO 5667-3:2012)BS EN ISO 5667-3:2012 BRITISH STANDARDNational forewordThis British

2、Standard is the UK implementation of EN ISO5667-3:2012. It supersedes BS EN ISO 5667-3:2003 which iswithdrawn.The UK participation in its preparation was entrusted to TechnicalCommittee EH/3/6, Sampling (of technical committee EH/3 - Waterquality).A list of organizations represented on this committe

3、e can beobtained on request to its secretary.This publication does not purport to include all the necessaryprovisions of a contract. Users are responsible for its correctapplication. The British Standards Institution 2012. Published by BSI StandardsLimited 2012ISBN 978 0 580 67477 8ICS 13.060.45Comp

4、liance with a British Standard cannot confer immunity fromlegal obligations.This British Standard was published under the authority of theStandards Policy and Strategy Committee on 31 December 2012.Amendments issued since publicationDate Text affectedEUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM

5、 EN ISO 5667-3 November 2012 ICS 13.060.45 Supersedes EN ISO 5667-3:2003English Version Water quality - Sampling - Part 3: Preservation and handling of water samples (ISO 5667-3:2012) Qualit de leau - chantillonnage - Partie 3: Conservation et manipulation des chantillons deau (ISO 5667-3:2012)Wasse

6、rbeschaffenheit - Probenahme - Teil 3: Konservierung und Handhabung von Wasserproben (ISO 5667-3:2012) This European Standard was approved by CEN on 14 November 2012. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Sta

7、ndard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (Engli

8、sh, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, B

9、ulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland,

10、 Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2012 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No.

11、 EN ISO 5667-3:2012: EBS EN ISO 5667-3:2012EN ISO 5667-3:2012 (E) 3 Foreword This document (EN ISO 5667-3:2012) has been prepared by Technical Committee ISO/TC 147 “Water quality“ in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN. This Euro

12、pean Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by May 2013, and conflicting national standards shall be withdrawn at the latest by May 2013. Attention is drawn to the possibility that some of the elements of

13、 this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 5667-3:2003. According to the CEN/CENELEC Internal Regulations, the national standards organisations of the following co

14、untries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,

15、Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 5667-3:2012 has been approved by CEN as a EN ISO 5667-3:2012 without any modification. BS EN ISO 5667-3:2012ISO 5667-3:2012(E) ISO 2012 All rights reserved iii

16、Contents PageForeword ivIntroduction vi1 Scope . 12 Normative references 13 Terms and definitions . 14 Sampling and chain of custody 25 Reagents and materials . 25.1 Solids . 35.2 Solutions . 35.3 Materials . 46 Containers . 46.1 Container selection and preparation . 46.2 Filtration on site . 56.3 F

17、illing the container . 57 Sample handling and preservation . 57.1 Sample handling and preservation for physical and chemical examination . 57.2 Sample handling and preservation for biological examination 67.3 Sample handling and preservation for radiochemical analysis 68 Sample transport . 79 Identi

18、fication of samples . 710 Sample reception . 811 Sample storage . 8Annex A (informative) Techniques for sample preservation 9Annex B (informative) Container preparation 35Annex C (informative) Protocol as used in Dutch validation studies .36Bibliography .38BS EN ISO 5667-3:2012ISO 5667-3:2012(E)Fore

19、wordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a techni

20、cal committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of e

21、lectrotechnical standardization.International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to th

22、e member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for iden

23、tifying any or all such patent rights.ISO 5667-3 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 6, Sampling (general methods).This fourth edition cancels and replaces the third edition (ISO 5667-3:2003), which has been technically revised.ISO 5667 consists of the foll

24、owing parts, under the general title Water quality Sampling : Part 1: Guidance on the design of sampling programmes and sampling techniques Part 3: Preservation and handling of water samples Part 4: Guidance on sampling from lakes, natural and man-made Part 5: Guidance on sampling of drinking water

25、from treatment works and piped distribution systems Part 6: Guidance on sampling of rivers and streams Part 7: Guidance on sampling of water and steam in boiler plants Part 8: Guidance on the sampling of wet deposition Part 9: Guidance on sampling from marine waters Part 10: Guidance on sampling of

26、waste waters Part 11: Guidance on sampling of groundwaters Part 12: Guidance on sampling of bottom sediments Part 13: Guidance on sampling of sludges Part 14: Guidance on quality assurance of environmental water-sampling and handling Part 15: Guidance on the preservation and handling of sludge and s

27、ediment samples Part 16: Guidance on biotesting of samples Part 17: Guidance on sampling of bulk suspended solids Part 19: Guidance on sampling of marine sedimentsiv ISO 2012 All rights reservedBS EN ISO 5667-3:2012ISO 5667-3:2012(E) Part 20: Guidance on the use of sampling data for decision making

28、Compliance with thresholds and classification systems Part 21: Guidance on sampling of drinking water distributed by tankers or means other than distribution pipes Part 22: Guidance on the design and installation of groundwater monitoring points Part 23: Guidance on passive sampling in surface water

29、s ISO 2012 All rights reserved vBS EN ISO 5667-3:2012ISO 5667-3:2012(E)IntroductionThis part of ISO 5667 is intended to be used in conjunction with ISO 5667-1, which deals with the design of sampling programmes and sampling techniques.Where possible this part of ISO 5667 has been brought into line w

30、ith current standards. Where new research or validation results have provided new insights, the latest knowledge has been used.Guidance on validation protocols can be found in ISO Guide 34.63vi ISO 2012 All rights reservedBS EN ISO 5667-3:2012INTERNATIONAL STANDARD ISO 5667-3:2012(E)Water quality Sa

31、mpling Part 3: Preservation and handling of water samplesNOTICE This part of ISO 5667 and the analytical International Standards listed in Annex A are complementary. Where no analytical International Standard is applicable, the technique(s) described in Tables A.1 to A.3 take(s) normative status.Whe

32、n new or revised analytical standards are developed with storage times or preservative techniques differing from those in Tables A.1 to A.3, then the storage times or preservative techniques should be validated and presented to ISO/TC 147/SC 6/WG 3 for incorporation into the next revision of this pa

33、rt of ISO 5667.1 ScopeThis part of ISO 5667 establishes general requirements for sampling, preservation, handling, transport and storage of all water samples including those for biological analyses. It is not applicable to water samples intended for microbiological analyses as specified in ISO 19458

34、, ecotoxicological assays, biological assays, and passive sampling as specified in the scope of ISO 5667-23.This part of ISO 5667 is particularly appropriate when spot or composite samples cannot be analysed on site and have to be transported to a laboratory for analysis.2 Normative referencesThe fo

35、llowing referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO 3696, Water for analytical laboratory use Specificat

36、ion and test methodsISO 5667 (all parts), Water quality SamplingISO 19458, Water quality Sampling for microbiological analysis3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.3.1integrityproperty that the parameter(s) of interest, information or con

37、tent of the sample container has not been altered or lost in an unauthorized manner or subject to loss of representativeness3.2sample preservationany procedure used to stabilize a sample in such a way that the properties under examination are maintained stable from the collection step until preparat

38、ion for analysisISO 11074:2005,294.4.20NOTE Different analytes may require several samples from the same source that are stabilized by different procedures. ISO 2012 All rights reserved 1BS EN ISO 5667-3:2012ISO 5667-3:2012(E)3.3sample storageprocess, and the result, of keeping a sample available un

39、der predefined conditions for a (usually) specified time interval between collection and further treatment of a sampleNOTE 1 Adapted from ISO 11074:2005,294.4.22.NOTE 2 Specified time is the maximum time interval.3.4storage timeperiod of time between filling of the sample container and further treat

40、ment of the sample in the laboratory, if stored under predefined conditionsNOTE 1 Sampling finishes as soon as the sample container has been filled with the sample. Storage time ends when the sample is taken by the analyst to start sample preparation prior to analysis.NOTE 2 Further treatment is, fo

41、r most analytes, a solvent extraction or acid destruction. The initial steps of sample preparation can be steps complementary to the storage conditions for the maintenance of analyte concentrations.4 Sampling and chain of custodyIf there is a need to take samples, this is done according to a samplin

42、g programme. The first step is to design a sampling programme. Guidance on this topic is given in ISO 5667-1.Depending on the sample type and matrix, the guidelines found in the relevant part(s) of ISO 5667 and ISO 19458 shall be consulted.The process of preservation and handling of water samples co

43、nsists of several steps. During this process, the responsibility for the samples might change. To ensure the integrity of the samples, all steps involving the sample shall be documented.All preparation procedures shall be checked to ensure positive or negative interferences do not occur. As a minimu

44、m, this shall include the analysis of blanks (e.g. field blank or sample container) or samples containing known levels of relevant analytes as specified in ISO 5667-14.5 Reagents and materialsWARNING Certain preservatives (e.g. acids, alkalis, formaldehyde) need to be used with caution. Sampling per

45、sonnel should be warned of potential dangers, and appropriate safety procedures should be followed.The following reagents are used for the sample preservation and shall only be prepared according to individual sampling requirements. All reagents used shall be of at least analytical reagent grade and

46、 water shall be of at least ISO 3696, grade 2. Acids referred to in this part of ISO 5667 are commercially available “concentrated” acids.All reagents shall be labelled with a “shelf-life”. The shelf-life represents the period for which the reagent is suitable for use, if stored correctly. This shel

47、f-life shall not be exceeded. Any reagents that are not completely used by the expiry of the shelf-life date shall be discarded.NOTE Often the shelf-life of reagents is supplied by the receiving laboratory.Check reagents periodically, e.g. by field blanks, and discard any reagent found to be unsuita

48、ble.Between on-site visits, reagents shall be stored separately from sample containers and other equipment in a clean, secure cabinet in order to prevent contamination.Each sample shall be labelled accordingly, after the addition of the preservative. Otherwise, there could be no visible indication a

49、s to which samples have been preserved, and which have not.2 ISO 2012 All rights reservedBS EN ISO 5667-3:2012ISO 5667-3:2012(E)5.1 Solids5.1.1 Sodium thiosulfate pentahydrate, Na2S2O35H2O, w(Na2S2O35H2O) 99 %.5.1.2 Ascorbic acid, C6H8O6, w(C6H8O6) 99 %.5.1.3 Sodium hydroxide, NaOH, w(NaOH) 99 %.5.1.4 Sodium tetraborate decahydrate, Na2B4O710H2O, w(Na2B4O710H2O), 99 %.CAUTION Sodium tetraborate decahydrate is known to be a carcinogen, mutagen and reproductive toxin (CMR).5.1.5 Hexamethylenetetramine (hexa