1、Microbiology of the food chain Horizontal method for detection and enumeration of Campylobacter spp.Part 2: Colony-count techniqueBS EN ISO 10272-2:2017BSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 10272-2 Ju
2、ly 2017 ICS 07.100.30 English Version Microbiology of the food chain - Horizontal method for detection and enumeration of Campylobacter spp. - Part 2: Colony-count technique (ISO 10272-2:2017) Microbiologie de la chane alimentaire - Mthode horizontale pour la recherche et le dnombrement de Campyloba
3、cter spp. - Partie 2 : Technique par comptage des colonies (ISO 10272-2:2017) Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zum Nachweis und zur Zhlung von Campylobacter - Teil 2: Koloniezhlverfahren (ISO 10272-2:2017) This European Standard was approved by CEN on 1 May 2017. CEN memb
4、ers are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on applicati
5、on to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Managem
6、ent Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
7、Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue
8、Marnix 17, B-1000 Brussels 2017 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 10272-2:2017 ENational forewordThis British Standard is the UK implementation of EN ISO 10272-2:2017. It is identical to ISO 10272-2:2017. It super
9、sedes DD ISO/TS 10272-2:2006, which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee AW/9, Microbiology.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the nece
10、ssary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2017 Published by BSI Standards Limited 2017ISBN 978 0 580 83262 8ICS 07.100.30Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard w
11、as published under the authority of the Standards Policy and Strategy Committee on 30 September 2017.Amendments/corrigenda issued since publicationDate Text affectedBRITISH STANDARDBS EN ISO 10272-2:2017EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 10272-2 July 2017 ICS 07.100.30 English
12、Version Microbiology of the food chain - Horizontal method for detection and enumeration of Campylobacter spp. - Part 2: Colony-count technique (ISO 10272-2:2017) Microbiologie de la chane alimentaire - Mthode horizontale pour la recherche et le dnombrement de Campylobacter spp. - Partie 2 : Techniq
13、ue par comptage des colonies (ISO 10272-2:2017) Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zum Nachweis und zur Zhlung von Campylobacter - Teil 2: Koloniezhlverfahren (ISO 10272-2:2017) This European Standard was approved by CEN on 1 May 2017. CEN members are bound to comply with t
14、he CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Manageme
15、nt Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status
16、 as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
17、Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 201
18、7 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 10272-2:2017 EBS EN ISO 10272-2:2017EN ISO 10272-2:2017 (E) 2 European foreword This document (EN ISO 10272-2:2017) has been prepared by Technical Committee ISO/TC 34 “Food prod
19、ucts” in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by January 201
20、8 and conflicting national standards shall be withdrawn at the latest by January 2018. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN shall not be held responsible for identifying any or all such patent rights. This document
21、has been prepared under a mandate given to CEN by the European Commission and the European Free Trade Association. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulga
22、ria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerl
23、and, Turkey and the United Kingdom. Endorsement notice The text of ISO 10272-2:2017 has been approved by CEN as EN ISO 10272-2:2017 without any modification. ISO 10272-2:2017(E)Foreword ivIntroduction v1 Scope . 12 Normative references 13 Terms and definitions . 14 Principle 24.1 General . 24.2 Prep
24、aration of dilutions 24.3 Enumeration . 24.4 Confirmation . 25 Culture media and reagents 26 Equipment and consumables 37 Sampling 38 Preparation of test sample . 39 Procedure. 49.1 Test portion, initial suspension and dilutions 49.2 Inoculation and incubation 49.3 Enumeration of characteristic colo
25、nies . 49.4 Confirmation of Campylobacter 49.4.1 General 49.4.2 Selection of colonies for confirmation 59.4.3 Examination of morphology and motility 59.4.4 Study of aerobic growth at 25 C . 59.4.5 Detection of oxidase activity 59.4.6 Interpretation . 59.5 Identification of Campylobacter species (opt
26、ional) . 69.5.1 General 69.5.2 Detection of catalase activity. 69.5.3 Detection of hippurate hydrolysis . 69.5.4 Detection of indoxyl acetate hydrolysis . 69.5.5 Interpretation . 710 Expression of results 711 Performance characteristics of the method . 711.1 Interlaboratory study 711.2 Repeatability
27、 limit 711.3 Reproducibility limit 812 Test report . 9Annex A (normative) Diagram of procedure .10Annex B (normative) Culture media and reagents .11Annex C (informative) Method validation studies and performance characteristics .16Bibliography .19 ISO 2017 All rights reserved iiiContents PageBS EN I
28、SO 10272-2:2017ISO 10272-2:2017(E)Foreword ivIntroduction v1 Scope . 12 Normative references 13 Terms and definitions . 14 Principle 24.1 General . 24.2 Preparation of dilutions 24.3 Enumeration . 24.4 Confirmation . 25 Culture media and reagents 26 Equipment and consumables 37 Sampling 38 Preparati
29、on of test sample . 39 Procedure. 49.1 Test portion, initial suspension and dilutions 49.2 Inoculation and incubation 49.3 Enumeration of characteristic colonies . 49.4 Confirmation of Campylobacter 49.4.1 General 49.4.2 Selection of colonies for confirmation 59.4.3 Examination of morphology and mot
30、ility 59.4.4 Study of aerobic growth at 25 C . 59.4.5 Detection of oxidase activity 59.4.6 Interpretation . 59.5 Identification of Campylobacter species (optional) . 69.5.1 General 69.5.2 Detection of catalase activity. 69.5.3 Detection of hippurate hydrolysis . 69.5.4 Detection of indoxyl acetate h
31、ydrolysis . 69.5.5 Interpretation . 710 Expression of results 711 Performance characteristics of the method . 711.1 Interlaboratory study 711.2 Repeatability limit 711.3 Reproducibility limit 812 Test report . 9Annex A (normative) Diagram of procedure .10Annex B (normative) Culture media and reagent
32、s .11Annex C (informative) Method validation studies and performance characteristics .16Bibliography .19 ISO 2017 All rights reserved iiiContents PageBS EN ISO 10272-2:2017ISO 10272-2:2017(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standa
33、rds bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organ
34、izations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its f
35、urther maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ di
36、rectives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in t
37、he Introduction and/or on the ISO list of patent declarations received (see www .iso .org/ patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions re
38、lated to conformity assessment, as well as information about ISOs adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: www .iso .org/ iso/ foreword .html.This document was prepared by the European Committee for Standardization (CE
39、N), Technical Committee CEN/TC 275, Food Analysis Horizontal methods, in collaboration with ISO Technical Committee ISO/TC 34, Food products, Subcommittee SC 9, Microbiology in accordance with the agreement on technical cooperation between ISO and CEN (Vienna Agreement).This first edition cancels an
40、d replaces ISO/TS 10272-2:2006, which has been technically revised with the following main changes: samples from the primary production stage have been added to the scope; serial dilutions are plated in single instead of in duplicate, to be in line with ISO 7218; the confirmation tests on study of m
41、icroaerobic growth at 25 C and aerobic growth at 41,5 C were replaced by the study of aerobic growth at 25 C; performance testing for the quality assurance of the culture media has been added to Annex B; performance characteristics have been added to Annex C.A list of all parts in the ISO 10272 seri
42、es can be found on the ISO website.iv ISO 2017 All rights reservedBS EN ISO 10272-2:2017ISO 10272-2:2017(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is nor
43、mally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in
44、the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular th
45、e different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).Attention is drawn to the possibility that some of the elements of this do
46、cument may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www .iso
47、 .org/ patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the W
48、orld Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: www .iso .org/ iso/ foreword .html.This document was prepared by the European Committee for Standardization (CEN), Technical Committee CEN/TC 275, Food Analysis Horizontal methods, in collaborati
49、on with ISO Technical Committee ISO/TC 34, Food products, Subcommittee SC 9, Microbiology in accordance with the agreement on technical cooperation between ISO and CEN (Vienna Agreement).This first edition cancels and replaces ISO/TS 10272-2:2006, which has been technically revised with the following main changes: samples from the primary production stage have been added to the scope; serial dilutions are plated in single instead of in duplicate, to be in line with ISO 7218; the confirmation tests on study of microaerobic growth at 25
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