BS EN ISO 16072-2011 Soil quality Laboratory methods for determination of microbial soil respiration《土壤质量 测定土壤微生物呼吸作用的实验室方法》.pdf

1、BRITISH STANDARDBS EN ISO 16072:2011Soil quality Laboratory methods for determination of microbial soil respirationICS 13.080.30g49g50g3g38g50g51g60g44g49g42g3g58g44g55g43g50g56g55g3g37g54g44g3g51g40g53g48g44g54g54g44g50g49g3g40g59g38g40g51g55g3g36g54g3g51g40g53g48g44g55g55g40g39g3g37g60g3g38g50g51g

2、60g53g44g42g43g55g3g47g36g58Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 24/11/2011 06:20, Uncontrolled Copy, (c) BSINational forewordThis British Standard is the UK implementation of EN ISO 16072:2011. It is identical to ISO 16072:2002. Itsupersedes BS ISO 16072:2002, which is withdrawn.T

3、he UK participation in its preparation was entrusted to Technical Committee EH/4, Soil quality.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsi

4、ble for its correct application. Compliance with a British Standard does cannot confer immunity from legal obligations.BS EN ISO 16072:2011This British Standard was published under the authority of the Standards Policy and Strategy Committee on31 January 2001 BSI 2011Amendments/corrigenda issued sin

5、ce publicationDate Comments 31 August 2011 This corrigendum renumbers BS ISO 16072:2002 asBS EN ISO 16072:2011ISBN 978 0 580 73153 2Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 24/11/2011 06:20, Uncontrolled Copy, (c) BSIEUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 16072 June 2

6、011 ICS 13.080.30 English Version Soil quality - Laboratory methods for determination of microbial soil respiration (ISO 16072:2002) Qualit du sol - Mthodes de laboratoire pour la dtermination de la respiration microbienne du sol (ISO 16072:2002) Bodenbeschaffenheit - Laborverfahren zur Bestimmung d

7、er mikrobiellen Bodenatmung (ISO 16072:2002) This European Standard was approved by CEN on 10 June 2011. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration

8、. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by t

9、ranslation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Fi

10、nland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES

11、 KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2011 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 16072:2011: ELicensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 24/11/2011 06:20, Uncontrolled Co

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16、obn the cnuotfo yr ttseuqer ehe.r ISO cirypothg fofice saCe tsopale 65 eneG 1121-HC 02 avleT. 4 + 10 947 22 1 11 xaF0 947 22 14 + 9 74 E-mial coirypthgis.o groWe bwww.is.o groii Foreword The text of ISO 16072:2002 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the Internationa

17、l Organization for Standardization (ISO) and has been taken over as EN ISO 16072:2011 by Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN. This European Standard shall be given the status of a national standard, either by publication of an identical

18、text or by endorsement, at the latest by December 2011, and conflicting national standards shall be withdrawn at the latest by December 2011. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held res

19、ponsible for identifying any or all such patent rights. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Fin

20、land, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. Endorsement notice The text of ISO 16072:2002 has been approved by CEN as a EN

21、 ISO 16072:2011 without any modification. BS EN ISO 16072:2011EN ISO 16072:2011 (E)Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 24/11/2011 06:20, Uncontrolled Copy, (c) BSIiiiContents Page Foreword iv Introduction v 1 Scope 1 2 Normative references . 1 3 Terms and definitions. 1 4 Procedur

22、e. 2 4.1 General conditions 2 4.2 Choice of the measuring system. 3 5 Measuring systems. 3 5.1 Determination of O2consumption by static incubation in a pressure-compensation system 3 5.2 Determination of CO2release by titration in a static system . 4 5.3 Coulometric determination of CO2release in a

23、static system . 6 5.4 Determination of CO2release using an infrared gas analyser in a flow-through system. 7 5.5 Determination of CO2release using gas chromatography in a flow-through system and a static system . 10 5.6 Determination of soil respiration by pressure measurement in a static system . 1

24、5 Bibliography . 19 BS EN ISO 16072:2011EN ISO 16072:2011 (E)Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 24/11/2011 06:20, Uncontrolled Copy, (c) BSIiv Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodi

25、es). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and

26、non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, P

27、art 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casti

28、ng a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 16072 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4,

29、 Biological methods. BS EN ISO 16072:2011EN ISO 16072:2011 (E)Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 24/11/2011 06:20, Uncontrolled Copy, (c) BSIvIntroduction This International Standard is derived from the German standard DIN 19737 (see 1). It describes methods for the determination

30、 of microbial soil respiration in the laboratory. Microbial soil respiration results from the mineralization of organic substances. In this process, organic substances are oxidized to the end products carbon dioxide and water, with concurrent uptake of O2for aerobic microorganisms. The soil respirat

31、ion is measured by the determination of O2consumption and/or by CO2release. Respiration is a measure of the overall activity of soil microorganisms. BS EN ISO 16072:2011EN ISO 16072:2011 (E)Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 24/11/2011 06:20, Uncontrolled Copy, (c) BSIBS EN ISO 1

32、6072:2011EN ISO 16072:2011 (E)Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 24/11/2011 06:20, Uncontrolled Copy, (c) BSI1Soil quality Laboratory methods for determination of microbial soil respiration 1 Scope This International Standard describes methods for the determination of soil microb

33、ial respiration of aerobic, unsaturated soils. The methods are suitable for the determination of O2uptake or CO2release, either after addition of a substrate (substrate-induced respiration), or without substrate addition (basal respiration). This International Standard is applicable to the measureme

34、nt of soil respiration in order to: determine the microbial activity in soil (see 3); establish the effect of additives (nutrients, pollutants, soil improvers, etc.) on the metabolic performance of microorganisms; determine the microbial biomass (see 4); determine the metabolic quotient qCO2. 2 Norm

35、ative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 10381-6:1993, Soil quality S

36、ampling Guidance on the collection, handling and storage of soil for the assessment of aerobic microbial processes in the laboratory ISO 11274:1998, Soil quality Determination of the water-retention characteristic Laboratory methods ISO 11465:1993, Soil quality Determination of dry matter and water

37、content on a mass basis Gravimetric method 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 basal respiration microbial soil respiration without addition of nutrients 3.2 substrate-induced respiration SIR microbial soil respiration after addit

38、ion of nutrients NOTE Glucose is an example of an added nutrient. BS EN ISO 16072:2011EN ISO 16072:2011 (E)Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 24/11/2011 06:20, Uncontrolled Copy, (c) BSI2 3.3 microbial activity metabolic performance of microorganisms NOTE It can be measured, for

39、example, as O2uptake or CO2release. 3.4 metabolic quotient qCO2specific metabolic activity of soil microorganisms, which can be calculated as the quotient basal respiration: microbial biomass NOTE Metabolic quotient is usually expressed as milligrams of CO2carbon released per hour per gram of microb

40、ial biomass carbon. 3.5 rate of CO2formation O2consumption RCO2 RO2 amount of CO2released O2consumed per time unit from a mass unit of soil NOTE 1 Soil respiration is usually measured as the rate of CO2formation or O2consumption. NOTE 2 It is usually expressed as milligrams CO2or O2 per gram per hou

41、r (mg CO2or O2g1h1). 3.6 microbial biomass mass of intact microbial cells in a given soil NOTE This is usually estimated from the measurement of carbon or nitrogen content of these cells. 4 Procedure 4.1 General conditions 4.1.1 Soil sampling and storage Sample, store and pre-incubate soils in accor

42、dance with ISO 10381-6, independently of the choice of the procedure and the respiration parameter to be measured (basal respiration, SIR). 4.1.2 Measuring and incubation conditions Soil respiration is strongly influenced by water content and temperature. Therefore these parameters should be recorde

43、d in the final report. At suction pressures 0,03 MPa, the soil respiration will decrease considerably. The water content of the test soil is optimal when it corresponds with a pore water pressure of 0,01 MPa to 0,03 MPa (measured with an accuracy of 5 %, in accordance with ISO 11274) or 40 % to 60 %

44、 of the maximum water-holding capacity, respectively. A stable temperature should be used. Incubation temperatures between 20 C and 30 C are generally recommended, but other temperatures may be used if required. In the description of the methods, examples of incubation temperatures are given as well

45、 as the accuracy of temperature maintenance and measurement. If a method is used for the determination of soil microbial biomass, a temperature of 22 C is recommended because biomass calculations have been calibrated to this temperature. When soil samples are compared with respect to soil respiratio

46、n, they should have the same moisture status (pore water pressure or percentage of maximum water-holding capacity). BS EN ISO 16072:2011EN ISO 16072:2011 (E)Licensed Copy: Wang Bin, ISO/EXCHANGE CHINA STANDARDS, 24/11/2011 06:20, Uncontrolled Copy, (c) BSI34.2 Choice of the measuring system Each mea

47、surement method has its own advantages and disadvantages. Care is needed, because the results obtained by O2uptake and by CO2release are not strictly compatible. It is the responsibility of the investigator to decide which of these methods is to be used. One of the systems described in Clause 5 shou

48、ld be used. Systems for measuring CO2do not distinguish between CO2released from microbial activities and CO2resulting from abiotic processes. For alkaline soils and soils with a high organic matter content, which can release considerable amounts of abiotically released CO2, methods using O2uptake a

49、re recommended. NOTE The advantages and disadvantages are described in the individual descriptions of the methods. 5 Measuring systems 5.1 Determination of O2consumption by static incubation in a pressure-compensation system 5.1.1 Principle The determination is based on the measurement of O2consumption during incubation of a soil sample in a closed system. The O2in the system is replenished electrochemically. The CO2released is absorbed by calcium hydroxide Ca(OH)2. Key A reaction vessel 1 soil sample 4 electrolyte B ox