ImageVerifierCode 换一换
格式:PDF , 页数:14 ,大小:199.81KB ,
资源ID:583441      下载积分:10000 积分
快捷下载
登录下载
邮箱/手机:
温馨提示:
如需开发票,请勿充值!快捷下载时,用户名和密码都是您填写的邮箱或者手机号,方便查询和重复下载(系统自动生成)。
如填写123,账号就是123,密码也是123。
特别说明:
请自助下载,系统不会自动发送文件的哦; 如果您已付费,想二次下载,请登录后访问:我的下载记录
支付方式: 支付宝扫码支付 微信扫码支付   
注意:如需开发票,请勿充值!
验证码:   换一换

加入VIP,免费下载
 

温馨提示:由于个人手机设置不同,如果发现不能下载,请复制以下地址【http://www.mydoc123.com/d-583441.html】到电脑端继续下载(重复下载不扣费)。

已注册用户请登录:
账号:
密码:
验证码:   换一换
  忘记密码?
三方登录: 微信登录  

下载须知

1: 本站所有资源如无特殊说明,都需要本地电脑安装OFFICE2007和PDF阅读器。
2: 试题试卷类文档,如果标题没有明确说明有答案则都视为没有答案,请知晓。
3: 文件的所有权益归上传用户所有。
4. 未经权益所有人同意不得将文件中的内容挪作商业或盈利用途。
5. 本站仅提供交流平台,并不能对任何下载内容负责。
6. 下载文件中如有侵权或不适当内容,请与我们联系,我们立即纠正。
7. 本站不保证下载资源的准确性、安全性和完整性, 同时也不承担用户因使用这些下载资源对自己和他人造成任何形式的伤害或损失。

版权提示 | 免责声明

本文(BS ISO 11023-2001 Liquorice extracts (Glycyrrhiza glabra L ) - Determination of glycyrrhizic acid content - Methods using high-performance liquid chromatography《甘草提取物 甘草酸含量的测定 高效液相.pdf)为本站会员(boatfragile160)主动上传,麦多课文库仅提供信息存储空间,仅对用户上传内容的表现方式做保护处理,对上载内容本身不做任何修改或编辑。 若此文所含内容侵犯了您的版权或隐私,请立即通知麦多课文库(发送邮件至master@mydoc123.com或直接QQ联系客服),我们立即给予删除!

BS ISO 11023-2001 Liquorice extracts (Glycyrrhiza glabra L ) - Determination of glycyrrhizic acid content - Methods using high-performance liquid chromatography《甘草提取物 甘草酸含量的测定 高效液相.pdf

1、BRITISH STANDARD BS ISO 11023:1999 Liquorice extracts (Glycyrrhiza glabra L.) Determination of glycyrrhizic acid content Method using high-performance liquid chromatography ICS 71.100.60; 71.040.40 NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBS ISO 11023:1999 This British S

2、tandard, having been prepared under the direction of the Consumer Products and Services Sector Committee, was published under the authority of the Standards Committee and comes into effect on 15 September 2001 BSI 08-2001 ISBN 0 580 38314 8 National foreword This British Standard reproduces verbatim

3、 ISO 11023:1999 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential Oils, which has the responsibility to: A list of organizations represented on this committee can be obtained on request to its secretary. Cross

4、-references The British Standards which implement international or European publications referred to in this document may be found in the BSI Standards Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Find” facility of the BSI Standards Electronic

5、 Catalogue. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to understand t

6、he text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document comprises a fro

7、nt cover, an inside front cover, the ISO title page, the ISO foreword page, pages 1 to 8, an inside back cover and a back cover. The BSI copyright date displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. Date CommentsA Referencenumbe r

8、 ISO11023:1999 (E) INTERNATIONAL STANDARD ISO 11023 Firstedition 19991015 Liquoriceextracts( Glycyrrhizaglabra L.) Determinationofglycyrrhizicacid contentMethodusinghighperformance liquidchromatography Extraitsderglisse( Glycyrrhizaglabra L.)Dterminationdelateneuren acideglycyrrhiziqueMthodeparchrom

9、atographieliquidehaute performanceISO11023:1999(E) OSI OSI9991 rllAightsreserved.Unelssotherwisespeceifid,noparotfhtsipubcilatoinamybereproducedorutziliednianyofrmorbyan ymsnae,lecetrinoc .rehsilbupehtmorfgnitirwninoissimreptuohtiw,mliforcimdnagniypocotohpgnidulcni,lacinahcemro noitazidradnatSrofnoi

10、tazinagrOlanoitanretnI 65elatsopesaC 02evneG1121HC dnalreztiwS tenretnI hc.osiosi dnalreztiwSnidetnirP ii Foreword ISO(theInternationalOrganizationforStandardization)isaworldwidefederationofnationalstandardsbodies(ISO memberbodies).TheworkofpreparingInternationalStandardsisnormallycarriedoutthroughI

11、SOtechnical committees.Eachmemberbodyinterestedinasubjectforwhichatechnicalcommitteehasbeenestablishedhas therighttoberepresentedonthatcommittee.Internationalorganizations,governmentalandnongovernmental,in liaisonwithISO,alsotakepartinthework.ISOcollaboratescloselywiththeInternationalElectrotechnica

12、l Commission(IEC)onallmattersofelectrotechnicalstandardization. InternationalStandardsaredraftedinaccordancewiththerulesgivenintheISO/IECDirectives,Part3. DraftInternationalStandardsadoptedbythetechnicalcommitteesarecirculatedtothememberbodiesforvoting. PublicationasanInternationalStandardrequiresap

13、provalbyatleast75%ofthememberbodiescastingavote. InternationalStandardISO11023waspreparedbyTechnicalCommitteeISO/TC54, Essentialoils . AnnexAofthisInternationalStandardisforinformationonly.INTERNATIONALSTANDARD OSI ISO11023:1999(E) 1 Liquoriceextracts( Glycyrrhizaglabra L.)Determinationof glycyrrhiz

14、icacidcontentMethodusinghighperformanceliquid chromatography 1Scope ThisInternationalStandarddescribesamethodfordeterminingtheglycyrrhizicacidcontentofliquoriceextract (Glycyrrhizaglabra L.)byhighperformanceliquidchromatography. Themethodisnotapplicabletoraworgroundliquoriceroot. 2Principle Thesampl

15、eandstandardsolutionsareprepared,thentheglycyrrhizicacidcontentisdeterminedbyhigh performanceliquidchromatographyusingthemethoddescribedinthisInternationalStandard. 3Reagents Useonlyreagentsofrecognizedanalyticalgrade,unlessotherwisespecified. 3.1 Water,HPLCgrade. 3.2 Referencesubstance ,monoammonia

16、calglycyrrhizate(GMA). Ifareferencematerialofguaranteedpurityisnotavailable,itisrecommendedthattheusersofthisInternational Standardcometoanagreementbetweentheinterestedpartiesonthepurityofthereferencesubstance. 3.3Acetonitrile ,HPLCgrade. 3.4 Aceticacid ,analyticalgrade. 3.5 Elutionsolvent(mobilepha

17、se), composedofthefollowing: 38volumesacetonitrile(3.3), 61volumeswater(3.1), 1volumeaceticacid(3.4). Usingthemeasuringcylinder(4.3),preparetheelutionsolventasfollows. Mix1volumeofaceticacidwith61volumesofwater,thenfilterthemixturethroughafilterforaqueoussolvents (4.5). Filter38volumesofacetonitrile

18、throughafilterfororganicsolvents(4.4). Addthefilteredacetonitriletothefilteredwater/aceticacidmixture.Mix,thendegastheelutionsolventintheultra soundcell(4.8.3)oranysuitablesystem. Donotkeepthissolventmorethan48hatambienttemperature.ISO11023:1999(E) OSI 2 4Apparatus Usuallaboratoryapparatusand,inpart

19、icular,thefollowing. 4.1 Pipettes,ofcapacities5mland10ml. 4.2 Volumetricflasks ,ofcapacities50mland100ml. 4.3 Measuringcylinder . 4.4 Filterfororganicsolvents ,0,5 m mporesize. 4.5 Filterforaqueoussolvents ,0,45 m mporesize. 4.6 Oven,capableofbeingmaintainedat105C+ 2C. 4.7 Analyticalbalance ,capable

20、ofweighingtothenearest0,0001g. 4.8 Separationsystem ,asfollows. 4.8.1 Chromatograph,highperformanceliquidphase. 4.8.2 Pumpingsystem ,forobtainingandmaintainingaconstantorprogrammedhighpressureflow. 4.8.3 Solventdegassingsystem ,suchasanultrasoundcelloranysuitablesystem. 4.8.4 Ultravioletdetectionsys

21、tem ,adjustabletoawavelengthof254nm. 4.9 Recorder orintegrator ,ofcompatibleperformancewithalltheapparatus. 4.10 Column,asfollows: material:stainlesssteelorglass; length:10cmto25cm; internaldiameter:0,4cmto0,5cm; stationaryphase:bondedphasesilicawithoctadecylC18derivedfunctionalgroup,maximumparticle

22、size 5 mm; columnefficiency:thenumberrecommendedoftheoreticalplatesis7000to10000. 4.11 Precolumn ,asfollows: material:stainlesssteel; length:10mmor25mm; internaldiameter:2mmor4mm; stationaryphase:bondedphasesilicawithoctadecylC18derivedfunctionalgroupOSI ISO11023:1999(E) 3 5Procedure 5.1 Preparation

23、ofstandardsolutions 5.1.1 Preparationofthe0,5mg/mlsolution Ina100mlvolumetricflask(4.2)weigh,tothenearest10 4 g,50mgofmonoammoniacalglycyrrhizate(3.2). Addtheelutionsolvent(3.5)anddissolvethemonoammoniacalglycyrrhizate(3.2).Makeuptothemarkwitha furtherquantityofelutionsolvent. Filterifnecessarythrou

24、ghtheorganicsolventfilter(4.4). Itisessentialtoprepareanewsolutioneveryday. 5.1.2 Preparationofdilutesolutionscontaining0,05mg/ml,0,075mg/mland0,1mg/ml 5.1.2.1 0,05mg/mlstandardsolution Usingapipette(4.1),transfer5mlofthe0,5mg/mlstandardsolution(5.1.1)toa50mlvolumetricflask(4.2).Make uptothemarkwith

25、theelutionsolvent(3.5). 5.1.2.2 0,075mg/mlstandardsolution Usingapipette(4.1),transfer15mlofthe0,5mg/mlstandardsolution(5.1.1)toa100mlvolumetricflask(4.2). Makeuptothemarkwiththeelutionsolvent(3.5). 5.1.2.3 0,1mg/mlstandardsolution Usingapipette(4.1),tranfer10mlofthe0,5mg/mlstandardsolution(5.1.1)to

26、a50mlvolumetricflask(4.2).Make uptothemarkwiththeelutionsolvent(3.5). Itisessentialtoprepareanewsolutioneveryday. 5.2 Preparationofthesolutionforanalysis 5.2.1 Preparethesolutionforanalysissuchthattheareaoftheglycyrrhizicacidpeakobtainedisbetweenthe peaksfortheothertwostandardsolutions,onthebasisoft

27、herecommendedmassesandvolumesspecifiedin Table1,accordingtotheassumedglycyrrhizicacidcontentofthesample. Table1Examplesofquantitiesandvolumestobeusedaccordingtotheassumedglycyrrhizicacid contentoftheliquoriceextracts Assumed glycyrrhizicacid content Massoftest portion Volumeofflask Volumeofpipetted

28、solution Volumeofflask V 1 V 2 V 3 %m gm lm lm l 3to5 1 50 5 50 5to10 1 100 5 50 10to15 0,50 100 5 50 20to25 0,25 100 5 50ISO11023:1999(E) OSI 4 5.2.2 Weigh,tothenearest10 -4 g,atestportionofmass m 0 intoavolumetricflaskofvolume V 1 ml. Addanappropriatequantityofelutionsolvent(3.5)todissolvethesampl

29、e.Thenmakeuptothemarkwithelution solvent. Usingapipette,transferavolume( V 2 )ofthissolutiontoavolumetricflaskofvolume V 3 ml. Addanappropriatequantityofelutionsolvent(3.5),mixandmakeuptothemarkwithelutionsolvent. Filterthislastsolutionthroughthefilterfororganicsolvents(4.3)priortoinjecting. 5.3 Det

30、erminationofthewaterandvolatilemattercontentofthesample Weigh,tothenearest10 -4 g,atestportion( m 0 )ofexactly2gandplaceinacalibratedwatchglass. Placeitintheoven(4.6)setat105Cfor24h,untilaconstantmassisreached.Coolthenweightheresidue( m 1 ). Thewaterandvolatilemattercontentofthesample, w,expressedas

31、apercentagebymass,isequalto: w mm m = - () % 01 0 100 where m 0 isthemassofthetestportion,ingrams; m 1 isthemassoftheresidueobtained,ingrams. 6Determination 6.1 Inject10 m lofsolution5.1.2.1. 6.2 Inject10 m lofsolution5.1.2.2. 6.3 Inject10 m lofsolution5.1.2.3. 6.4 Inject10 m lofthesolutionforanalys

32、is(5.2.2). 6.5 Carryouttheoperationsdescribedin6.1to6.4twice. NOTE Iftheinjectionanddetectionsystemsallowthis,itispossibletoinjectquantitiesfrom5 m lto10 m l. 6.6 Measuretheareaoftheglycyrrhizicacidpeakonthechromatogramsobtainedforthestandardsolutions(see annexA). 6.7 Plotacurveofglycyrrhizicacidpea

33、kareaagainsttheconcentrationofmonoammoniacalglycyrrhizateinthe standardsolutions. Acalibrationlinepassingthroughtheoriginisobtained. 6.8 Usingthechromatogramforthesamplesolution,measuretheareaoftheglycyrrhizicacidpeak. Fromthecalibrationline,readthemonoammoniacalglycyrrhizateconcentration, c,inmilli

34、gramspermillilitre.OSI ISO11023:1999(E) 5 7 Expressionofresults Theglycyrrhizicacidcontent,w G , expressedasapercentagebymassondrymatter,iscalculatedusingthe formula: w cVV p mV w G () = - 13 02 100 822 100 839 where m 0 isthemassofthetestportion,ingrams; c isthemeanvalueoftheconcentrationsobtained,

35、inmilligramspermillilitre,ofmonoammoniacal glycyrrhizatereadfromthecalibrationline; p isthepurityofthereferencesubstance,inpercent; w isthewaterandvolatilemattercontentofthesampleforanalysis,asapercentagebymass,determinedin 5.3; 822 isthemolarmassofglycyrrhizicacid; 839 isthemolarmassofmonoammoniaca

36、lglycyrrhizate; V 1 , V 2 , V 3 havethesamemeaningsasindicatedinTable1. 8Test report Thetestreportshallspecify: allinformationnecessaryforthecompleteidentificationofthesample; thesamplingmethodused,ifknown; thetestmethodused,withreferencetothisInternationalStandard; alloperatingdetailsnotspecifiedin

37、thisInternationalStandard,orregardedasoptional,togetherwithdetailsof anyincidentswhichmayhaveinfluencedthetestresult(s); thetestresult(s)obtained;or iftherepeatabilityhasbeenchecked,thefinalquotedresultobtained.ISO11023:1999(E) OSI 6 AnnexA (informative) Examplesofchromatograms FigureA.1Chromatogram

38、ofaliquoriceextractOSI ISO11023:1999(E) 7 FigureA.2ChromatogramofthereferencesubstanceISO11023:1999(E) OSI 8 Bibliography 1 ISO8432, EssentialoilsAnalysisbyhighperformanceliquidchromatographyGeneralmethod.BS ISO 11023:1999 BSI 389 Chiswick High Road London W4 4AL BSI British Standards Institution BS

39、I is the independent national body responsible for preparing British Standards. It presents the UK view on standards in Europe and at the international level. It is incorporated by Royal Charter. Revisions British Standards are updated by amendment or revision. Users of British Standards should make

40、 sure that they possess the latest amendments or editions. It is the constant aim of BSI to improve the quality of our products and services. We would be grateful if anyone finding an inaccuracy or ambiguity while using this British Standard would inform the Secretary of the technical committee resp

41、onsible, the identity of which can be found on the inside front cover. Tel: 020 8996 9000. Fax: 020 8996 7400. BSI offers members an individual updating service called PLUS which ensures that subscribers automatically receive the latest editions of standards. Buying standards Orders for all BSI, int

42、ernational and foreign standards publications should be addressed to Customer Services. Tel: 020 8996 9001. Fax: 020 8996 7001. Standards are also available from the BSI website at http:/www.bsi-. In response to orders for international standards, it is BSI policy to supply the BSI implementation of

43、 those that have been published as British Standards, unless otherwise requested. Information on standards BSI provides a wide range of information on national, European and international standards through its Library and its Technical Help to Exporters Service. Various BSI electronic information se

44、rvices are also available which give details on all its products and services. Contact the Information Centre. Tel: 020 8996 7111. Fax: 020 8996 7048. Subscribing members of BSI are kept up to date with standards developments and receive substantial discounts on the purchase price of standards. For details of these and other benefits contact Membership Administration. Tel: 020 8996 7002. Fax: 020 8996 7001. Further information about BSI is availabl

copyright@ 2008-2019 麦多课文库(www.mydoc123.com)网站版权所有
备案/许可证编号:苏ICP备17064731号-1