BS ISO 3033-4-2005 Oil of spearmint - Scotch variety (Mentha X gracilis Sole)《留兰香油 苏格兰变种(Mentha X gracilis Sole)》.pdf

1、BRITISH STANDARD BS ISO 3033-4:2005 Oil of spearmint Sole) ICS 71.100.60 Part 4: Scotch variety (Mentha gracilis BS ISO 3033-4:2005 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 7 November 2005 BSI 7 November 2005 ISBN 0 580 46747 3 Nationa

2、l foreword This British Standard reproduces verbatim ISO 3033-4:2005 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils, which has the responsibility to: aid enquirers to understand the text; present to th

3、e responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep UK interests informed; monitor related international and European developments and promulgate them in the UK. A list of organizations represented on this committee can be obtained on

4、 request to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electron

5、ic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This

6、 document comprises a front cover, an inside front cover, the ISO title page, pages ii and iii, a blank page, pages 1 to 6, an inside back cover and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd.

7、 No. Date Comments Reference number ISO 3033-4:2005(E)INTERNATIONAL STANDARD ISO 3033-4 First edition 2005-09-01 Oil of spearmint Part 4: Huile essentielle de menthe crpue (ou menthe verte) Partie 4: Varit Scotch (Mentha gracilis Sole) Scotch variety (Mentha gracilis Sole) BS ISO 3033-4:2005ii iii F

8、oreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a te

9、chnical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters

10、of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated

11、 to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible f

12、or identifying any or all such patent rights. ISO 3033-4 was prepared by Technical Committee ISO/TC 54, Essential oils. This first edition of ISO 3033-4, together with ISO 3033-1:2005, ISO 3033-2:2005 and ISO 3033-3:2005, cancels and replaces ISO 3033:1988, which has been technically revised. ISO 30

13、33 consists of the following parts, under the general title Oil of spearmint: Part 1: Native type (Mentha spicata L.) Part 2: Chinese type (80 % and 60 %) (Mentha viridis L. var. crispa Benth.), redistilled oil Part 3: Indian type (Mentha spicata L.), redistilled oil Part 4: Scotch variety (Mentha g

14、racilis Sole) BS ISO 3033-4:2005blank1 Oil of spearmint Part 4: Scotch variety (Mentha gracilis Sole) 1 Scope This part of ISO 3033 specifies certain characteristics of the oil of spearmint, Scotch variety (Mentha gracilis Sole), in order to facilitate assessment of its quality. 2 Normative referenc

15、es The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for p

16、ackaging, conditioning and storage ISO/TR 211, Essential oils General rules for labelling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 592, E

17、ssential oils Determination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 1271, Essential oils Determination of carbonyl value Free hydroxylamine method ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatograp

18、hic profiles for presentation in standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 o

19、il of spearmint, Scotch variety essential oil obtained by steam distillation from the fresh above-ground parts of the flowering plant of Mentha gracilis Sole, of Lamiaceae family NOTE For information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1 Appearance Clear mobile liquid. 4.2 Colour F

20、rom colourless to pale yellow. 4.3 Odour Characteristic odour of carvone with a herbaceous note. 4.4 Relative density at 20 C, 20 20 d Minimum: 0,921 0 Maximum: 0,938 0 4.5 Refractive index at 20 C Minimum: 1,484 0 Maximum: 1,491 0 4.6 Optical rotation at 20 C Between 59 and 48. BS ISO 3033-4:2005BS

21、 ISO 3033-4:2005 2 4.7 Miscibility in ethanol, 70 % (volume fraction), at 20 C It shall not be necessary to use more than 3 volumes of ethanol, 70 % (volume fraction), to obtain a clear solution with 1 volume of essential oil. 4.8 Carbonyl value Minimum: 224, corresponding to a carbonyl compound con

22、tent of 60 %, expressed as carvone. 4.9 Chromatographic profile Analysis of the essential oil shall be carried out by gas chromatography. In the chromatogram obtained, the representative and characteristic components shown in Table 1 shall be identified. The proportions of these components, indicate

23、d by the integrator, shall be as shown in Table 1. This constitutes the chromato- graphic profile of the essential oil. Table 1 Chromatographic profile Component Minimum % Maximum % Limonene a 11,5 16,5 3-Octanol 0,6 1,4 Menthone b 0,15 trans-Sabinene hydrate 0,5 1,0 cis-Dihydrocarvone 1,0 2,5 Carvo

24、ne c 60,0 70,0 trans-Dihydrocarvyl acetate 0,1 0,6 cis-Carvyl acetate 0,1 0,6 cis-Jasmone 0,2 0,7 -Bourbonene 1,0 2,0 Viridiflorol Not detectable aThe limonene found is regarded to be predominantly L-limonene based on the physical testing. It is believed that there might be a small amount of D-limon

25、ene present but the exact quantity is unknown. bThe menthone found is regarded to be predominantly L-menthone based on the physical testing. It is believed that there might be a small amount of D-menthone present but the exact quantity is unknown. cThe carvone found is regarded to be predominantly L

26、-carvone based on the physical testing. It is believed that there might be a small amount of D-carvone present but the exact quantity is unknown. NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A. 4.10 Flashpoint Information on the flas

27、hpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 50 ml. NOTE This volume allows each of the tests specified in this part of ISO 3033 to be carried out at least once. 6 Test methods 6.1 Relative density at 20 C, 20 20 d See ISO 279. 6.2 Refractive index at 20 C See I

28、SO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Miscibility in ethanol, 70 % (volume fraction), at 20 C See ISO 875. 6.5 Carbonyl value See ISO 1271. Test portion: 1 g. Reflux time: 3 h. Relative molar mass of carvone: 150,21. 6.6 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Pack

29、aging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BS ISO 3033-4:2005 3 Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of spearmint, Scotch variety (Mentha gracilis Sole) Peak identification Operating conditions 1 3-Octanol 2

30、Limonene 3 trans-Sabinene hydrate 4 Menthone 5 cis-Dihydrocarvone 6 Carvone 7 trans-Dihydrocarvyl acetate 8 cis-Carvyl acetate 9 cis-Jasmone 10 -Bourbonene 11 Viridiflorol Column: fused silica capillary; length 30 m; internal diameter 0,25 mm Stationary phase: poly(dimethyl siloxane) (DB-1 ) Film th

31、ickness: 0,25 m Oven temperature: isothermal at 75 C for 5 min, then temperature programming from 75 C to 100 C at a rate of 5 C/min, then from 100 C to 220 C at a rate of 6 C/min and isothermal at 220 C for 8,34 min Injector temperature: 230 C Detector temperature: 260 C Detector: flame ionization

32、type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/100 Figure A.1 Typical chromatogram taken on an apolar column 4 Peak identification Operating conditions 1 Limonene 2 3-Octanol 3 Menthone 4 trans-Sabinene hydrate 5 -Bourbonene 6 cis-Dihydrocarvone 7 tran

33、s-Dihydrocarvyl acetate 8 Carvone 9 cis-Carvyl acetate 10 cis-Jasmone 11 Viridiflorol (not detected) Column: fused silica capillary; length 30 m; internal diameter 0,25 mm Stationary phase: poly(ethylene glycol) (DB-WAX ) Film thickness: 0,25 m Oven temperature: isothermal at 75 C for 5 min, then te

34、mperature programming from 75 C to 100 C at a rate of 5 C/min, then from 100 C to 220 C at a rate of 6 C/min and isothermal at 220 C for 8,34 min Injector temperature: 230 C Detector temperature: 260 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate:

35、1 ml/min Split ratio: 1/100 Figure A.2 Typical chromatogram taken on a polar column BS ISO 3033-4:20055 Annex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies, and people in charge of safety services require information on the flashpoint

36、s of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that: there is wide variation in the chemical composition of

37、 essential oils; the volume of the sample needed in certain requirements would be too costly for high- priced essential oils; ss there are several different types of equipment which can be used for the determination, users cannot be expected to use one specified type only. Consequently, it was decid

38、ed to give a mean value for the flashpoint in an informative annex to each International Standard in order to meet the requirements of the interested parties. The equipment with which this value was obtained should be specified. For further information, see ISO/TR 11018. B.2 Flashpoint of the essent

39、ial oil of spearmint, Scotch variety The mean value is +58 C. NOTE Obtained with “Setaflash“ equipment. BS ISO 3033-4:20056 Bibliography 1 ISO/TR 11018, Essential oils General guidance on the determination of flashpoint 2 ISO/TR 21092, Essential oils Characterization BS ISO 3033-4:2005blankBS ISO 30

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