BS ISO 3517-2012 Essential oil of neroli (Citrus aurantium L syn Citrus amara Link syn Citrus bigaradia Loisel syn Citrus vulgaris Risso)《橙花精油(酸橙Citrus aurantium L syn 柑橘苦味药链Citru.pdf

1、raising standards worldwideNO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBSI Standards PublicationBS ISO 3517:2012Essential oil of neroli (Citrus aurantium L., syn. Citrus amara Link, syn. Citrus bigaradia Loisel, syn. Citrus vulgaris Risso)BS ISO 3517:2012 BRITISH STANDARDNa

2、tional forewordThis British Standard is the UK implementation of ISO 3517:2012. The UK participation in its preparation was entrusted to T e c h n i c a l Committee AW/54, Essential oils.A list of organizations represented on this committee can be obtained on request to its secretary.This publicatio

3、n does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2012Published by BSI Standards Limited 2012ISBN 978 0 580 66093 1 ICS 71.100.60 Compliance with a British Standard cannot confer immunity fro

4、m legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 August 2012.Amendments issued since publicationDate T e x t a f f e c t e dBS ISO 3517:2012 ISO 2012Essential oil of neroli (Citrus aurantium L., syn. Citrus amara Link, s

5、yn. Citrus bigaradia Loisel, syn. Citrus vulgaris Risso)Huile essentielle de nroli bigaradier (Citrus aurantium L., syn. Citrus amara Link, syn. Citrus bigaradia Loisel, syn. Citrus vulgaris Risso)INTERNATIONAL STANDARDISO3517Third edition2012-08-01Reference numberISO 3517:2012(E)BS ISO 3517:2012ISO

6、 3517:2012(E)ii ISO 2012 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2012All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission

7、in writing from either ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCase postale 56 CH-1211 Geneva 20Tel. + 41 22 749 01 11Fax + 41 22 749 09 47E-mail copyrightiso.orgWeb www.iso.orgPublished in SwitzerlandBS ISO 3517:2012ISO 3517:2012(E) ISO 2012

8、All rights reserved iiiContents PageForeword iv1 Scope 12 Normative references . 13 Terms and definitions . 14 Requirements . 14.1 Appearance . 14.2 Colour . 24.3 Odour 24.4 Relative density at 20 C, d2020. 24.5 Refractive index at 20 C . 24.6 Optical rotation at 20 C . 24.7 Miscibility with 85 % vo

9、lume fraction ethanol at 20 C . 24.8 Acid value 24.9 Chromatographic profile . 24.10 Chirality 24.11 Flashpoint 25 Sampling 36 Test methods 36.1 Relative density at 20 C, d2020. 36.2 Refractive index at 20 C . 36.3 Optical rotation at 20 C . 36.4 Miscibility in ethanol, 85 % volume fraction at 20 C

10、. 36.5 Acid value 46.6 Chromatographic profile . 46.7 Chirality 47 Packaging, labelling and marking . 4Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of neroli (Citrus aurantium L., syn. Citrus amara Link, syn. Citrus bigaradia Loisel, syn. Cit

11、rus vulgaris Risso) . 5Annex B (informative) Flashpoint . 8Annex C (informative) Chirality . 9Bibliography .10BS ISO 3517:2012ISO 3517:2012(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of prepa

12、ring International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in l

13、iaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.The main task of

14、technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.Attention is d

15、rawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights.ISO 3517 was prepared by Technical Committee ISO/TC 54, Essential oils.This third edition cancels and replaces the s

16、econd edition (ISO 3517:2002), which has been technically revised.iv ISO 2012 All rights reservedBS ISO 3517:2012INTERNATIONAL STANDARD ISO 3517:2012(E)Essential oil of neroli (Citrus aurantium L., syn. Citrus amara Link, syn. Citrus bigaradia Loisel, syn. Citrus vulgaris Risso)1 ScopeThis Internati

17、onal Standard specifies certain characteristics of the essential oil of neroli (Citrus aurantium L., syn. Citrus amara Link, syn. Citrus bigaradia Loisel, syn. Citrus vulgaris Risso), cultivated principally in Tunisia, Morocco, and Egypt, in order to facilitate assessment of its quality.2 Normative

18、referencesThe following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO/TR 210, Essential oils General rules

19、 for packaging, conditioning and storageISO/TR 211, Essential oils General rules for labelling and marking of containersISO 212, Essential oils SamplingISO 279, Essential oils Determination of relative density at 20 C Reference methodISO 280, Essential oils Determination of refractive indexISO 592,

20、Essential oils Determination of optical rotationISO 875, Essential oils Evaluation of miscibility with ethanolISO 1242, Essential oils Determination of acid valueISO 11024 (all parts), Essential oils General guidance on chromatographic profilesISO 22972, Essential oils Analysis by gas chromatography

21、 on chiral capillary columns General method3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.3.1essential oil of neroliessential oil obtained by steam distillation or hydrodistillation of the flowers of Citrus aurantium L., syn. Citrus amara Link, sy

22、n. Citrus bigaradia Loisel, syn. Citrus vulgaris Risso, of the Rutaceae familyNOTE For information on the CAS number, see ISO/TR 21092.24 Requirements4.1 AppearanceClear mobile liquid. ISO 2012 All rights reserved 1BS ISO 3517:2012ISO 3517:2012(E)4.2 ColourPale yellow to amber with a slight blue flu

23、orescence.4.3 OdourFresh, recalling that of orange blossom.4.4 Relative density at 20 C, d2020Tunisia and Morocco EgyptMinimum: 0,863 Minimum: 0,870Maximum: 0,876 Maximum: 0,8804.5 Refractive index at 20 CTunisia and Morocco EgyptMinimum: 1,464 Minimum: 1,465Maximum: 1,474 Maximum: 1,4724.6 Optical

24、rotation at 20 CTunisia and Morocco EgyptBetween +2 and +12 Between +2 and +114.7 Miscibility with 85 % volume fraction ethanol at 20 CIt shall not be necessary to use more than 3,5 volumes of 85 % volume fraction ethanol to obtain a clear solution with 1 volume of essential oil.4.8 Acid valueMaximu

25、m: 2.4.9 Chromatographic profileCarry out the analysis of the essential oil by gas chromatography. Identify in the chromatogram obtained, the representative and characteristic components shown in Table 1. The proportions of these components, indicated by the integrator, shall be as shown in Table 1.

26、 This constitutes the chromatographic profile of the essential oil.4.10 ChiralityInformation on the chirality is given in Annex C.4.11 FlashpointInformation on the flashpoint is given in Annex B.2 ISO 2012 All rights reservedBS ISO 3517:2012ISO 3517:2012(E)Table 1 Chromatographic profileComponentTun

27、isia and Morocco Egyptmin. max. min. max.% % % %-Pinene n.d.a2,0 n.d.a2,0Sabinene n.d.a3,0 n.d.a3,0-Pinene 5,0 17,0 2,0 8,0Myrcene 1,0 4,0 1,0 4,0Limonene 9,0 18,0 7,0 17,0(E)-Ocimene 3,0 9,0 3,0 9,0Linalol 26,0 48,0 26,0 55,0-Terpineol 2,0 5,5 2,0 8,0Linalyl acetate 1,5 15,0 3,0 20,0Neryl acetate n

28、.d.a2,5 n.d.a7,0Geranyl acetate 1,0 5,0 1,0 5,0(E)-Nerolidol 1,0 5,0 0,5 5,0(2E,6E)-Farnesol 0,5 4,0 0,5 4,0Nerol 0,9 1,2 0,5 2,0Geraniol 2,5 3,1 1,0 5,0Indole 0,1 0,3 n.d.a0,5Methyl anthranilate 0,1 1,0 n.d.a1,0NOTE The chromatographic profile is normative, contrary to typical chromatograms given f

29、or information in Annex A.aNot detectable.5 SamplingSampling shall be performed in accordance with ISO 212.Minimum volume of the final sample: 30 ml.NOTE This volume allows each of the tests specified in this International Standard to be carried out at least once.6 Test methods6.1 Relative density a

30、t 20 C, d2020Determine the relative density in accordance with ISO 279.6.2 Refractive index at 20 CDetermine the refractive index in accordance with ISO 280.6.3 Optical rotation at 20 CDetermine the optical rotation in accordance with ISO 592.6.4 Miscibility in ethanol, 85 % volume fraction at 20 CD

31、etermine the miscibility in accordance with ISO 875. ISO 2012 All rights reserved 3BS ISO 3517:2012ISO 3517:2012(E)6.5 Acid valueDetermine the acid value in accordance with ISO 1242.6.6 Chromatographic profileDetermine the chromatographic profile in accordance with ISO 11024.6.7 ChiralityDetermine t

32、he chirality in accordance with ISO 22972.7 Packaging, labelling and markingThese items shall be in accordance with ISO/TR 210 and ISO/TR 211.4 ISO 2012 All rights reservedBS ISO 3517:2012ISO 3517:2012(E)Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essenti

33、al oil of neroli (Citrus aurantium L., syn. Citrus amara Link, syn. Citrus bigaradia Loisel, syn. Citrus vulgaris Risso) ISO 2012 All rights reserved 5BS ISO 3517:2012ISO 3517:2012(E)Peak identification Operating conditions1 -Thujene Column: silica capillary; length 30 m; internal diameter 0,25 mm2

34、-Pinene Stationary phase: poly(dimethylsiloxane) (SE 30a)3 Sabinene Film thickness: 0,25 m4 -PineneOven temperature: temperature programming from 70 C to 250 C at a rate of 2 C/min5 Myrcene6 Limonene Injector temperature: 250 C7 (Z)-Ocimene Detector temperature: 250 C8 (E)-Ocimene Detector: flame io

35、nization type9 -Terpinene Carrier gas: hydrogen10 Linalol + phenylacetronitrile Volume injected: 0,3 l11 -Terpineol Carrier gas flow rate: 1 ml/min12 Nerol Split ratio: 1/10013 Geraniol 14 Linalyl acetate t time15 Indole 16 Methyl anthranilateaSE 30 is an example of a suitable product available comm

36、ercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product. 17 Terpenyl acetate18 Neryl acetate19 Geranyl acetate 20 (E)-Nerolidol 21 (2E,6E)-Farnesol Figure A.1 Typical chromatogram taken on an apolar column6 ISO 2

37、012 All rights reservedBS ISO 3517:2012ISO 3517:2012(E)Peak identification Operating conditions1 -Thujene + -pinene Column: silica capillary; length 30 m; internal diameter 0,25 mm2 -Pinene Stationary phase: poly(ethyleneglycol) 10 000 (Supelcowax 10a)3 Sabinene Film thickness: 0,25 m4 MyrceneOven t

38、emperature: temperature programming from 70 C to 250 C at a rate of 2 C/min5 Limonene6 (Z)-Ocimene Injector temperature: 250 C7 -Terpinene Detector temperature: 250 C8 (E)-Ocimene Detector: flame ionization type9 Linalol Carrier gas: nitrogen10 Linalyl acetate Volume injected: 0,3 l11 -Terpineol + T

39、erpenyl acetate Carrier gas flow rate: 1 ml/min12 Neryl acetate Split ratio: 1/10013 Geranyl acetate 14 Nerol t time15 Geraniol16 PhenylacetronitrileaSupelcowax 10 is an example of a suitable product available commercially. This information is given for the convenience of users of this document and

40、does not constitute an endorsement by ISO of this product. 17 (E)-Nerolidol18 Methyl anthranilate19 (2E,6E)-Farnesol 20 Indole Figure A.2 Typical chromatogram taken on a polar column ISO 2012 All rights reserved 7BS ISO 3517:2012ISO 3517:2012(E)Annex B (informative) FlashpointB.1 General information

41、For safety reasons, transport companies, insurance companies, and people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products.A comparative study on the relevant methods of analysis (see ISO/TR 110181) concluded that it was

42、 difficult to recommend a single apparatus for standardization purposes, given that: there is a wide variation in the chemical composition of essential oils; the volume of the sample needed in certain requirements would be too costly for high-priced essential oils; as there are several different typ

43、es of equipment which can be used for the determination, users cannot be expected to use one specified type only.Consequently, it was decided to give a mean value for the flashpoint annexed to each International Standard, for information, in order to meet the requirements of the interested parties.T

44、he equipment with which this value was obtained has to be specified.For further information, see ISO/TR 11018.1B.2 Flashpoint of the essential oil of neroliThe indicative value is +61 C.NOTE Obtained with “Luchaire”1)equipment.1) Equipment available commercially. This information is given for the co

45、nvenience of users of this document and does not constitute an endorsement by ISO of this product.8 ISO 2012 All rights reservedBS ISO 3517:2012ISO 3517:2012(E)Annex C (informative) ChiralityC.1 Enantiomeric distributionPerform the analysis of essential oils by gas chromatography on capillary chiral

46、 columns. The relative content of each of these enantiomers shall be situated within the limits given in Table C.1.Table C.1 Enantiomeric distribution of the chiral compoundsComponent DistributionS-(+)-Linalyl acetate 5 % ISO 2012 All rights reserved 9BS ISO 3517:2012ISO 3517:2012(E)Bibliography1 IS

47、O/TR 11018, Essential oils General guidance on the determination of flashpoint2 ISO/TR 21092, Essential oils Characterization10 ISO 2012 All rights reservedBS ISO 3517:2012BS ISO 3517:2012ISO 3517:2012(E) ISO 2012 All rights reservedICS 71.100.60Price based on 10 pagesThis page deliberately left bla

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