BS ISO 4730-2017 Essential oil of Melaleuca terpinen-4-ol type (Tea Tree oil)《松油烯-4-醇型白千层精油(茶树油)》.pdf

1、BS ISO 4730:2017Essential oil of Melaleuca,terpinen-4-ol type (Tea Tree oil)BSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06BS ISO 4730:2017 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 4730:2017. It supersedes BS ISO 4730:20

2、04 which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a co

3、ntract. Users are responsible for its correct application. The British Standards Institution 2017.Published by BSI Standards Limited 2017ISBN 978 0 580 92042 4 ICS 71.100.60 Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the

4、 authority of the Standards Policy and Strategy Committee on 28 February 2017.Amendments/corrigenda issued since publicationDate T e x t a f f e c t e dBS ISO 4730:2017 ISO 2017Essential oil of Melaleuca, terpinen-4-ol type (Tea Tree oil)Huile essentielle de Melaleuca, type terpinn-4-ol (huile essen

5、tielle de Tea Tree)INTERNATIONAL STANDARDISO4730Third edition2017-02Reference numberISO 4730:2017(E)BS ISO 4730:2017ISO 4730:2017(E)ii ISO 2017 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2017, Published in SwitzerlandAll rights reserved. Unless otherwise specified, no part of this publicati

6、on may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISOs member body in the country o

7、f the requester.ISO copyright officeCh. de Blandonnet 8 CP 401CH-1214 Vernier, Geneva, SwitzerlandTel. +41 22 749 01 11Fax +41 22 749 09 47copyrightiso.orgwww.iso.orgBS ISO 4730:2017ISO 4730:2017(E)Foreword iv1 Scope . 12 Normative references 13 Terms and definitions . 14 Requirements 25 Additional

8、information 35.1 Flashpoint 35.2 Enantiomeric distribution . 36 Sampling 37 Packaging, labelling, marking and storage . 3Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of Melaleuca, terpinen-4-ol type (Tea Tree oil) 4Annex B (informative) Flash

9、point . 8Annex C (informative) Enantiomeric distribution General information . 9Bibliography .10 ISO 2017 All rights reserved iiiContents PageBS ISO 4730:2017ISO 4730:2017(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO m

10、ember bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governm

11、ental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenanc

12、e are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).Attenti

13、on is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction a

14、nd/or on the ISO list of patent declarations received (see www .iso .org/ patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the voluntary nature of standards, the meaning of ISO specific terms

15、and expressions related to conformity assessment, as well as information about ISOs adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: www .iso .org/ iso/ foreword .html.This document was prepared by Technical Committee ISO/TC 5

16、4, Essential oils.This third edition cancels and replaces the second edition (ISO 4730:2004), which has been technically revised.iv ISO 2017 All rights reservedBS ISO 4730:2017INTERNATIONAL STANDARD ISO 4730:2017(E)Essential oil of Melaleuca, terpinen-4-ol type (Tea Tree oil)1 ScopeThis document spe

17、cifies certain characteristics of the essential oil of Melaleuca, terpinen-4-ol type (Tea Tree oil), in order to facilitate assessment of its quality.2 Normative referencesThe following documents are referred to in the text in such a way that some or all of their content constitutes requirements of

18、this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO/TS 210, Essential oils General rules for packaging, conditioning and storageISO/TS 211, Essential oils General rules for l

19、abelling and marking of containersISO 212, Essential oils SamplingISO 279, Essential oils Determination of relative density at 20 C Reference methodISO 280, Essential oils Determination of refractive indexISO 592, Essential oils Determination of optical rotationISO 875, Essential oils Evaluation of

20、miscibility in ethanolISO 11024 (all parts), Essential oils General guidance on chromatographic profiles3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.ISO and IEC maintain terminological databases for use in standardization at the following addres

21、ses: IEC Electropedia: available at http:/ www .electropedia. org/ ISO Online browsing platform: available at http:/ www. iso. org/ obp3.1essential oil of Melaleuca, terpinen-4-ol typeTea Tree oilessential oil obtained by steam distillation of the leaves and terminal branchlets of Melaleuca alternif

22、olia (Maiden et Betche) Cheel or of M. linariifolia Sm.Note 1 to entry: For information on the CAS number, see ISO/TR 21092. ISO 2017 All rights reserved 1BS ISO 4730:2017ISO 4730:2017(E)4 Requirements4.1 Essential oil of Melaleuca, terpinen-4-ol type (Tea Tree oil) shall meet the requirements as gi

23、ven in Table 1.Table 1 Requirements for the essential oil of Melaleuca, terpinen-4-ol type (Tea Tree oil)Characteristics Requirements ISO test methodAppearance Clear, mobile liquid Colour Colourless to pale yellow Odour Characteristic Relative density at 20 C, d20200,885 to 0,906 ISO 279Refractive i

24、ndex at 20 C 1,475 to 1,482 ISO 280Optical rotation Between +7 and +12 ISO 592Miscibility in ethanol 85 % (volume fraction) at 20 CIt shall not be necessary to use more than 2 volumes of ethanol, 85 % (volume fraction), to obtain a clear solution with 1 volume of essential oilISO 8754.2 Chromatograp

25、hic profileCarry out the analysis of the essential oil by gas chromatography. Determine the chromatographic profile in accordance with the ISO 11024 series. Identify in the chromatogram obtained, the representative and characteristic components shown in Table 2. The proportions of these components,

26、indicated by the integrator, shall be as shown in Table 2. This constitutes the chromatographic profile of the essential oil.Table 2 Chromatographic profileComponentsMin. Max.% %-Pinene 1,0 4,0Sabinene tracesa3,5-Terpinene 6,0 12,0Limonene 0,5 1,5p-Cymene 0,5 8,01,8-Cineole tracesa10,0-Terpinene 14,

27、0 28,0Terpinolene 1,5 5,0Terpinen-4-ol 35,0 48,0-Terpineol 2,0 5,0Aromadendrene 0,2 3,0Ledene (syn. viridiflorene) 0,1 3,0-Cadinene 0,2 3,0Globulol tracesa1,0Viridiflorol tracesa1,0atraces: 0,01 %.NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information

28、in Annex A.2 ISO 2017 All rights reservedBS ISO 4730:2017ISO 4730:2017(E)5 Additional information5.1 FlashpointInformation on the flashpoint is given in Annex B.5.2 Enantiomeric distributionInformation concerning enantiomeric distribution is given in Annex C.6 SamplingSampling shall be performed in

29、accordance with ISO 212. Minimum volume of test sample is 50 ml.NOTE This volume allows each of the tests specified in this document to be carried out at least once.7 Packaging, labelling, marking and storageThese items shall be in accordance with ISO/TS 210 and ISO/TS 211. ISO 2017 All rights reser

30、ved 3BS ISO 4730:2017ISO 4730:2017(E)Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of Melaleuca, terpinen-4-ol type (Tea Tree oil)4 ISO 2017 All rights reservedBS ISO 4730:2017ISO 4730:2017(E)Peak identification Operating conditions1 -Thujene

31、Column: fused silica capillary; length 50 m; internal diameter 0,20 mm2 -Pinene Stationary phase: poly(dimethyl siloxane) (OV-101 a)3 Sabinene Film thickness: 0,25 m4 -Pinene Oven temperature: temperature programming from 70 C to 220 C at a rate of5 Myrcene 2 C/min6 -Phellandrene Injector temperatur

32、e: 230 C7 -Terpinene Detector temperature: 250 C8 p-Cymene Detector: flame ionization type9 1,8-Cineole + -phellandrene Carrier gas: hydrogen10 Limonene Volume injected: 0,2 l11 -Terpinene Carrier gas flow rate: 1,0 ml/min12 Terpinolene Split ratio: 1/10013 Terpinen-4-ol14 -Terpineol15 Aromadendrene

33、16 Ledene (viridiflorene)17 -Cadinene18 Globulol19 ViridiflorolaOV-101is an example of a suitable product available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product.Figure A.1 Typical chromatogram take

34、n on an apolar column ISO 2017 All rights reserved 5BS ISO 4730:2017ISO 4730:2017(E)Peak identification Operating conditions1 -Thujene Column: FSOT; length 60 m; internal diameter 0,25 mm2 -Pinene Stationary phase: (35 %)-diphenyl-(65 %)-methylsiloxane copolymer3 Sabinene (AT-35 a)4 -Pinene Film thi

35、ckness: 0,25 m5 Myrcene Oven temperature: isothermal at 50 C for 1 min, then temperature6 -Phellandrene programming from 50 C to 250 C at a rate of 10 C/min and isothermal7 -Terpinene at 250 C for 9 min8 Limonene Injector temperature: 200 C9 -Phellandrene Detector temperature: 300 C10 p-Cymene Detec

36、tor: flame ionization type11 1,8-Cineole Carrier gas: hydrogen12 -Terpinene Volume injected: 1 l (1 % in ethanol)13 Terpinolene Carrier gas flow rate: 1,0 ml/min14 Terpinen-4-ol Split ratio: 1/5015 -Terpineol16 Aromadendrene17 Ledene (viridiflorene)18 -Cadinene19 Globulol20 ViridiflorolaAT-35 is an

37、example of a suitable product available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product.Figure A.2 Typical chromatogram taken on an intermediate polarity column6 ISO 2017 All rights reservedBS ISO 473

38、0:2017ISO 4730:2017(E)Peak identification Operating conditions1 -Pinene Column: fused silica capillary; length 50 m; internal diameter 0,33 mm2 -Thujene Stationary phase: poly(ethylene glycol) 20 000 (BP-20 a)3 -Pinene Film thickness: 0,50 m4 Sabinene Oven temperature: isothermal at 50 C for 1 min,

39、then temperature5 Myrcene programming from 50 C to 220 C at a rate of 5 C/min and isothermal at6 -Phellandrene 220 C for 5 min7 -Terpinene Injector temperature: 240 C8 Limonene Detector temperature: 240 C9 -Phellandrene Detector: flame ionization type10 1,8-Cineole Carrier gas: helium11 -Terpinene V

40、olume injected: 1,5 l (3 % in hexane)12 p-Cymene Carrier gas flow rate: 1,0 ml/min13 Terpinolene Split ratio: 1/10014 Terpinen-4-ol15 Aromadendrene16 -Terpineol17 Ledene (viridiflorene)18 -Cadinene19 Globulol20 ViridiflorolaBP-20is an example of a suitable product available commercially. This inform

41、ation is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product.Figure A.3 Typical chromatogram taken on a polar column ISO 2017 All rights reserved 7BS ISO 4730:2017ISO 4730:2017(E)Annex B (informative) FlashpointB.1 General informationFor

42、safety reasons, transport companies, insurance companies and people in charge of safety services require information on the flash points of essential oils, which in most cases are flammable products.A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was diff

43、icult to recommend a single apparatus for standardization purposes, given that: there is a wide variation in the chemical composition of essential oils; the volume of the sample needed in certain requirements would be too costly for high-priced essential oils. as there are several different types of

44、 equipment which can be used for the determination, users cannot be expected to use one specified type only.Consequently, it was decided to give a mean value for the flashpoint annexed to each document, for information, in order to meet the requirements of the interested parties.The equipment with w

45、hich this value was obtained shall be specified. For further information, see ISO/TR 11018.B.2 Flashpoint of the essential oil of Melaleuca, terpinen-4-ol (Tea Tree oil)The mean value is +59 C.NOTE Obtained with “closed cup” equipment.8 ISO 2017 All rights reservedBS ISO 4730:2017ISO 4730:2017(E)Ann

46、ex C (informative) Enantiomeric distribution General informationSome essential oil components can exist in two enantiomeric forms designated as (R) or (S), D or L or (+) or (-) isomers. Many enantiomers have distinctly different properties and hence their presence in the right form is critical. Also

47、, pure natural essential oils contain enantiomers in characteristic ratios. This ratio is upset by the addition of adulterants including synthetic major components of different enantiomeric ratios.Consequently, the measurement of enantiomeric excess or enantiomeric ratio as per ISO 229724in an infor

48、mative annex of appropriate isolates in International Standards, provides an extra measure of essential oil authenticity.The enantiomeric distribution for terpinen-4-ol is (R)(+) 67 % - 71 % and (S)(-) 29 % - 33 %. ISO 2017 All rights reserved 9BS ISO 4730:2017ISO 4730:2017(E)Bibliography1 ISO 3218,

49、 Essential oils Principles of nomenclature2 ISO/TR 11018, Essential oils General guidance on the determination of flashpoint3 ISO/TR 21092, Essential oils Characterization4 ISO 22972, Essential oils Analysis by gas chromatography on chiral capillary columns General method10 ISO 2017 All rights reservedBS ISO 4730:2017BS ISO 4730:2017ISO 4730:2017(E) ISO 2017 All rights reservedICS 71.100.60Price based o