BS ISO 856-2006 Oil of peppermint (Mentha x piperita L )《薄荷油(Mentha x piperita L )》.pdf

1、 g49g50g3g38g50g51g60g44g49g42g3g58g44g55g43g50g56g55g3g37g54g44g3g51g40g53g48g44g54g54g44g50g49g3g40g59g38g40g51g55g3g36g54g3g51g40g53g48g44g55g55g40g39g3g37g60g3g38g50g51g60g53g44g42g43g55g3g47g36g58ICS 71.100.60Oil of peppermint (Mentha piperita L.)BRITISH STANDARDBS ISO 856:2006BS ISO 856:2006Th

2、is British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 May 2006 BSI 2006ISBN 0 580 48334 7The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “In

3、ternational Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue or of British Standards Online.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a

4、British Standard does not of itself confer immunity from legal obligations.Summary of pagesThis document comprises a front cover, an inside front cover, the ISO title page, pages ii to iv, pages 1 to 12, an inside back cover and a back cover.The BSI copyright notice displayed in this document indica

5、tes when the document was last issued.Amendments issued since publicationAmd. No. Date CommentsA list of organizations represented on this committee can be obtained on request to its secretary.Cross-referencesenquiries on the interpretation, or proposals for change, and keep UK interests informed; m

6、onitor related international and European developments and promulgate them in the UK.National forewordThis British Standard reproduces verbatim ISO 856:2006 and implements it as the UK national standard.The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oil

7、s, which has the responsibility to: aid enquirers to understand the text; present to the responsible international/European committee any Reference numberISO 856:2006(E)INTERNATIONAL STANDARD ISO856Second edition2006-04-15Oil of peppermint (Mentha piperita L.) Huile essentielle de menthe poivre (Men

8、tha piperita L.) BS ISO 856:2006ii iiiContents Page Foreword iv 1 Scope . 1 2 Normative references . 1 3 Terms and definitions. 1 4 Requirements 1 4.1 Appearance . 1 4.2 Colour 1 4.3 Odour . 1 4.4 Relative density at 20 C, d2020. 1 4.5 Refractive index at 20 C 1 4.6 Optical rotation at 20 C . 2 4.7

9、Miscibility in ethanol, 70 % (volume fraction), at 20 C 2 4.8 Acid value 2 4.9 Chromatographic profile 2 4.10 Flashpoint 2 5 Sampling 3 6 Test methods. 3 6.1 Relative density at 20 C, d2020. 3 6.2 Refractive index at 20 C 3 6.3 Optical rotation at 20 C . 3 6.4 Miscibility in ethanol, 70 % (volume fr

10、action), at 20 C 3 6.5 Acid value 3 6.6 Chromatographic profile 3 7 Packaging, labelling, marking and storage 3 Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the oil of peppermint (Mentha piperita L.). 4 Annex B (informative) Flashpoint . 11 Bibliography . 12 B

11、S ISO 856:2006iv Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subj

12、ect for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IE

13、C) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committ

14、ees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be

15、held responsible for identifying any or all such patent rights. ISO 856 was prepared by Technical Committee ISO/TC 54, Essential oils. This second edition cancels and replaces the first edition (ISO 856:1981), which has been technically revised. BS ISO 856:20061Oil of peppermint (Mentha piperita L.)

16、 1 Scope This International Standard specifies certain characteristics of the oil of peppermint (Mentha piperita L.), with a view to facilitate assessment of its quality. NOTE As it is difficult to differentiate the specification of peppermint oils (Mentha piperita L.) from different origins, they h

17、ave been regrouped in this International Standard. The following origins have been taken into consideration: USA, United Kingdom, France, Italy, India and China. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, on

18、ly the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General rules for labelling and marking of containers IS

19、O 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 1242,

20、 Essential oils Determination of acid value ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for presentation in standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatog

21、raphic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 oil of peppermint essential oil obtained by steam distillation of the aerial parts of the plant Mentha piperita L., of the Lamiaceae family NOTE For

22、information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1 Appearance Clear, mobile liquid. 4.2 Colour Almost colourless to pale greenish yellow. 4.3 Odour Characteristic of mint, sweet and menthol-like, with some variations depending on the origin. 4.4 Relative density at 20 C, d2020Origin

23、s other than US US type Min. 0,898 0,899 Max. 0,918 0,911 4.5 Refractive index at 20 C Minimum: 1,459 0 Maximum: 1,465 0 BS ISO 856:20062 4.6 Optical rotation at 20 C Origins other than US US type 30 to 14 32 to 18 4.7 Miscibility in ethanol, 70 % (volume fraction), at 20 C For origins other than US

24、, it shall not be necessary to use more than 5 volumes of ethanol, 70 % (volume fraction), to obtain a clear solution with 1 volume of essential oil. For the US type, it shall not be necessary to use more than 3 volumes of ethanol, 70 % (volume fraction), to obtain a clear solution with 1 volume of

25、essential oil. 4.8 Acid value Maximum: 2 4.9 Chromatographic profile Analysis of the essential oil shall be carried out by gas chromatography. In the chromatogram obtained, the representative and characteristics components shown in Table 1 shall be identified. The proportions of these components, in

26、dicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil. 4.10 Flashpoint Information on the flashpoint is given in Annex B. Table 1 Chromatographic profile Origins other than US US type Components Min. (%) Max. (%) Min. (%) Max. (%)

27、3-Octanol 0,1 0,5 0,1 0,4 1,8-Cineole 3,0 8,0 4,0 6,0 Limonene a1,0 3,0 1,0 2,5 trans-Sabinene hydrate 0,5 2,0 0,5 2,3 Menthone 13,0 28,0 15,0 25,0 Isomenthone 2,0 8,0 2,0 4,5 Menthofuran 1,0 8,0 1,5 6,0 Neomenthol 2,0 6,0 2,5 4,5 Menthol 32,0 49,0 36,0 46,0 Pulegone 0,5 3 0,5 2,5 Menthyl acetate b2

28、,0 8,0 3,0 6,5 -Caryophyllene 1,0 3,5 1,0 2,5 NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A. aThe limonene is regarded to be predominantly L-limonene based on the physical tests. It is believed that there might be a small amount of

29、D-limonene present but the exact quantity is unknown. bThe menthyl acetate is regarded to be predominantly L-menthyl acetate based on the physical tests. It is believed that there might be a small amount of D-menthyl acetate present but the exact quantity is unknown. BS ISO 856:200635 Sampling See I

30、SO 212. Minimum volume of final sample: 25 ml. NOTE This volume allows each of the tests specified in this International Standard to be carried out at least once. 6 Test methods 6.1 Relative density at 20 C, d2020See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See

31、 ISO 592. 6.4 Miscibility in ethanol, 70 % (volume fraction), at 20 C See ISO 875. 6.5 Acid value See ISO 1242. 6.6 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BS ISO 856:20064 Annex A (informative) Typical chrom

32、atograms of the analysis by gas chromatography of the oil of peppermint (Mentha piperita L.) BS ISO 856:20065A.1 Typical chromatograms of the analysis of the oil of peppermint, France (Provence) Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 12 3-Octanol + myrcene 1,8-Cineole Limon

33、ene trans-Sabinene hydrate Menthone Isomenthone Menthofuran Neomenthol Menthol Pulegone Menthyl acetate -Caryophyllene Column: silica capillary; length 50 m; internal diameter 0,2 mm Stationary phase: poly(dimethyl siloxane) Film thickness: 0,25 m Oven temperature: temperature programming from 65 C

34、to 230 C at a rate of 2 C/min Injector temperature: 230 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l Carrier gas flow rate: 1,1 ml/min Split ratio: 1/100 Figure A.1 Typical chromatogram taken on an apolar column BS ISO 856:20066 Peak iden

35、tification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 12 Limonene 1,8-Cineole 3-Octanol Menthone trans-Sabinene hydrate Menthofuran Isomenthone Menthyl acetate -Caryophyllene Neomenthol + terpinen-4-ol Pulegone Menthol Column: silica capillary; length 50 m; internal diameter 0,2 mm Stationary phas

36、e: poly(ethylene glycol) (Carbowax 20 M) Film thickness: 0,25 m Oven temperature: temperature programming from 65 C to 230 C at a rate of 2 C/minInjector temperature: 230 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l Carrier gas flow rate:

37、 1,1 ml/min Split ratio: 1/100 Figure A.2 Typical chromatogram taken on a polar column BS ISO 856:20067A.2 Typical chromatograms of the analysis of the oil of peppermint, India Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 12 13 3-Octanol Myrcene 1,8-Cineole Limonene trans-Sabinen

38、e hydrate Menthone Isomenthone Menthofuran Neomenthol Menthol Pulegone Menthyl acetate -Caryophyllene Column: silica capillary; length 50 m; internal diameter 0,2 mm Stationary phase: poly(dimethyl siloxane) Film thickness: 0,25 m Oven temperature: temperature programming from 65 C to 230 C at a rat

39、e of 2 C/minInjector temperature: 230 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l Carrier gas flow rate: 1,1 ml/min Split ratio: 1/100 Figure A.3 Typical chromatogram taken on an apolar column BS ISO 856:20068 Peak identification Operati

40、ng conditions 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Myrcene -Terpinene Limonene 1,8-Cineole 3-Octanol trans-Sabinene hydrate Menthone Menthofuran Isomenthone Menthyl acetate Isoneo-isopulegol Neomenthol -Caryophyllene Menthol Pulegone Column: silica capillary; length 60 m; internal diameter 0,2 mm Sta

41、tionary phase: poly(ethylene glycol) (Supelcowax 10) Film thickness: 0,20 m Oven temperature: temperature programming from 80 C to 180 C at a rate of 1 C/minInjector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,1 l Carrier gas

42、flow rate: 30 ml/min Split ratio: 1/100 Figure A.4 Typical chromatogram taken on a polar column BS ISO 856:20069A.3 Typical chromatogram of the analysis of the oil of peppermint, USA (Idaho) Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Myrcene -Terpinene Limonene 1,8-

43、Cineole 3-Octanol trans-Sabinene hydrate Menthone Menthofuran Isomenthone Menthyl acetate Isoneo-isopulegol Neomenthol -Caryophyllene Menthol Pulegone Column: silica capillary; length 60 m; internal diameter 0,2 mm Stationary phase: poly(ethylene glycol) (Supelcowax 10) Film thickness: 0,20 m Oven t

44、emperature: temperature programming from 80 C to 180 C at a rate of 1 C/minInjector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate: 30 ml/min Split ratio: 1/100 Figure A.5 Typical chromatogram taken on a

45、 polar column BS ISO 856:200610 A.4 Typical chromatogram of the analysis of the oil of peppermint, USA (Willamette) Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Myrcene -Terpinene Limonene 1,8-Cineole 3-Octanol trans-Sabinene hydrate Menthone Menthofuran Isomenthone M

46、enthyl acetate Isoneo-isopulegol Neomenthol -Caryophyllene Menthol Pulegone Column: silica capillary; length 60 m; internal diameter 0,2 mm Stationary phase: poly(ethylene glycol) (Supelcowax 10) Film thickness: 0,20 m Oven temperature: temperature programming from 80 C to 180 C at a rate of 1 C/min

47、Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate: 30 ml/min Split ratio: 1/100 Figure A.6 Typical chromatogram taken on a polar column BS ISO 856:200611Annex B (informative) Flashpoint B.1 General

48、 information For safety reasons, transport companies, insurance companies and people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded

49、 that it was difficult to recommend a single apparatus for standardization purposes, given that: there is wide variation in the chemical composition of essential oils; the volume of the sample needed for certain requirements would be too costly for high-priced essential oils; as there are several different types of equipment which can be used for the determination, users cannot be expected to use one specifi