CAN CGSB-4 2 NO 28 2-M91-1991 Textile test methods Resistance to micro-organisms - Surfacegrowing fungus test - Pure culture.pdf

1、 CAN/CGSB-4.2 No. 28.2-M91 Supersedes CAN/CGSB-4.2 Method 28.2 July 1977 Extended April 1997 Reaffirmed November 2013 National Standard of Canada ICS 59.080.01 Textile test methods Resistance to micro-organisms Surface-growing fungus test Pure culture The CANADIAN GENERAL STANDARDS BOARD (CGSB), und

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9、d in its Scope, it is important to note that it remains the responsibility of the users of the standard to judge its suitability for their particular purpose. The testing and evaluation of a product against this standard may require the use of materials and/or equipment that could be hazardous. This

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21、ion from the SDO that publishes the standard. The responsibility for approving standards as NSCs rests with: Standards Council of Canada 270 Albert Street, Suite 200 Ottawa, Ontario K1P 6N7, CANADA How to order Publications: by telephone 819-956-0425 or 1-800-665-2472 by fax 819-956-5740 by mail CGS

22、B Sales Centre Gatineau, Canada K1A 1G6 in person Place du Portage Phase III, 6B1 11 Laurier Street Gatineau, Quebec by email ncr.cgsb-ongctpsgc-pwgsc.gc.ca on the Web www.tpsgc-pwgsc.gc.ca/ongc-cgsb NATIONAL STANDARD OF CANADA CAN/CGSB-4.2 No. 28.2-M91 Supersedes CAN/CGSB-4.2 Method 28.2 July 1977

23、Extended April 1997 Reaffirmed November 2013 Textile test methods Resistance to micro-organisms Surface-growing fungus test Pure culture CETTE NORME NATIONALE DU CANADA EST DISPONIBLE EN VERSIONS FRANAISE ET ANGLAISE. Published August 1991 by the Canadian General Standards Board Gatineau, Canada K1A

24、 1G6 Minister of Supply and Services Canada 1991 No part of this publication may be reproduced in any form without the prior permission of the publisher. Prepared by the Canadian General Standards Board Approved by the CAN/CGSB-4.2 No. 28.2-M91 CGSB 2013 All rights reserved CANADIAN GENERAL STANDARD

25、S BOARD Committee on Textile Test Methods and Terminology (Voting membership at date of reaffirmation) General interest category Batcheller, J. University of Alberta Carrick, D. Consultant Davie, N. Consultant Liu, S. University of Manitoba Man, T.M. Consultant Tait, C. National Defence/DSSPM Produc

26、er category Adam, C. Tencate Protective Fabrics Canada Boivin, D. E.I. DuPont Co. Lawson, L. Davey Textile Solutions Leblanc, J.-M. Marv Holland Apparel Ltd. Schumann, E. Lincoln Fabrics Ltd. Taylor, V. Invista (Canada) Co. Regulator category Andersson, C. Health Canada User category Bourget, S. Nat

27、ional Defence/QETE DEntremont, E. Royal Canadian Mounted Police Izquierdo, V. Textile Technologies Centre Kohli, G. Sears Canada Inc. Larsen, A.-L. Exova Group Ltd. Litva, M. Canada Border Services Agency MacLeod, J. Public Works and Government Services Canada Tebbs, C. International Drycleaners Con

28、gress Secretary (non-voting) Grabowski, M. Canadian General Standards Board Acknowledgment is made for the translation of this National Standard of Canada by the Translation Bureau of Public Works and Government Services Canada. CGSB 2013 All rights reserved i CAN/CGSB-4.2 No. 28.2-M91 Supersedes CA

29、N/CGSB-4.2 Method 28.2 July 1977 Extended April 1997 Reaffirmed November 2013 Preface to the National Standard of Canada This National Standard of Canada has been extended and reaffirmed by the CGSB Committee on Textile Test Methods and Terminology. It is published without editorial changes. Superse

30、des CAN/CGSB-4.2Method 28.2July 1977ExtendedApril 1997Reaffirmed1RYember 20133.6 Petri dishes: 100 mm in diameter. 3.7 Leaching Apparatus This shall consist of a reservoir of water at 24 + 3OC from which water is passed to a suitable vessel containing the fabric to be leached. The size of this vesse

31、l will depend on the size of the sample to be leached. The rate of flow of water through the vessel should be such as to provide approximately four changes of water per hour. The water used must not contain any material that may react with any of the constituents of the sample being tested (Note 3).

32、 Wherever possible, distilled or demineralized water shall be used. The pH of the water shall not be less than 5.0 nor greater than 8.0. TEST SPECIMENS Fabrics - A minimum of five specimens (40 mm diameter circles or 50 x 50 mm squares) taken from widely distributed areas of the sample are required.

33、 Yarns - A minimum of five representative specimens each approximately 500 mm long and wound into loops approximately 50 mm long are required. If the textile is expected to be used outdoors, the specimens shall be leached for 24 h (Note 4). PROCEDURE Sterilize the required number of 100 mm Petri dis

34、hes under the conditions specified in par. 3.4. Sterilize the nutrient agar solution, pour into the sterile Petri dishes, and allow to stand until hardened. Using a sterile loop of nichrome, tungsten or platinum wire, add scrapings from a ripe fruiting culture of the organism, 7 d old and sufficient

35、 to cover a 100 mm Petri dish, to an Erlenmeyer flask containing 100 mL of sterile water. Shake the suspension of spore clusters in the flask with sterile glass beads until the spores are in suspension. To maintain a uniform suspension, the addition of a surface-active agent is recommended (Note 5).

36、 Other equally satisfactory procedures for making spore suspensions may be used. Inoculate the surface of the agar with approximately 1.0 mL of spore suspension. Place the specimen on the seeded agar. Using a pipette, inoculate the specimen with 0.2 to 0.4 mL of spore suspension. Incubate the Petri

37、dishes and contents for two weeks at 30 + 2OC. Keep the specimens under observation during that period, noting the extent of growth on the specimens and on the agar medium adjacent to the specimens, as well as the presence or absence of fruiting bodies. At the end of the incubation period, remove th

38、e specimens, wash them in water, and dry at room temperature. Then examine for staining, and also (in the case of dyed materials) for change of colour. REPORT Report the following: The extent of growth on and around the specimens, together with any staining or colour change. Note 3: An example of th

39、e use of an unsuitable type of water is to employ a water containing alkaline impurities to leach a sample carrying a phenolic type fungicide. Depending on the degree of alkalinity, solubilization of the fungicide by the alkali in the water will occur through the formation of soluble phenate, and th

40、e loss thus occasioned would not represent a loss through water solubility of the original phenolic compound. Note 4: To facilitate wetting by the spore suspension, it is desirable that the inoculation of the specimens (section 5) be proceeded with immediately following leaching, before the specimen

41、s have become dry. Where drying has occurred, the specimens should be dampened by being thoroughly sprayed with distilled water and allowed to stand for 1 to 2 h, flat, on a non- absorbent surface in a closed container, to avoid dessication. Note 5: The product Aerosol OT (sodium di-octyl sulphosuccunate) at a concentration of 0.05% is suitable. 6.2 The number of this method: CANICGSB-4.2 No. 28.2-M91.