DIN EN 49-1-2016 en 8819 Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 1 Applicati.pdf

1、November 2016 English price group 13No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 71.100.50!%ud“2588265www.din.de

2、DIN EN 49-1Wood preservatives Determination of the protective effectiveness against Anobium punctatum (De Geer) by egglaying and larval survival Part 1: Application by surface treatment (Laboratory method);English version EN 491:2016,English translation of DIN EN 49-1:2016-11Holzschutzmittel Bestimm

3、ung der vorbeugenden Wirkung gegenber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des berlebens von Larven Teil 1: Oberflchenverfahren (Laboratoriumsverfahren);Englische Fassung EN 491:2016,Englische bersetzung von DIN EN 49-1:2016-11Produits de prservation du bois Dtermination de

4、lefficacit protectrice visvis dAnobium punctatum (De Geer) par lobservation de la ponte et du taux de survie des larves Partie 1: Application par traitement de surface (Mthode de laboratoire);Version anglaise EN 491:2016,Traduction anglaise de DIN EN 49-1:2016-11SupersedesDIN EN 491:200506www.beuth.

5、deDocument comprises 24 pagesDTranslation by DIN-Sprachendienst.In case of doubt, the German-language original shall be considered authoritative.11.16 DIN EN 49-1:2016-11 2 A comma is used as the decimal marker. National foreword This document (EN 49-1:2016) has been prepared by Technical Committee

6、CEN/TC 38 “Durability of wood and wood-based products” (Secretariat: AFNOR, France). The responsible German body involved in its preparation was DIN-Normenausschuss Holzwirtschaft und Mbel (DIN Standards Committee Timber and Furniture), Working Committee NA 042-03-06 AA Spiegelausschuss zu CEN/TC 38

7、 und ISO/TC 165/SC 1 Dauerhaftigkeit von Holz und Holzprodukten. Amendments This standard differs from DIN EN 49-1:2005-06 as follows: a) the material specifications for preparing the egg-laying zones have been unified; b) the requirements for wood quality have been unified. Previous editions DIN EN

8、 49: 1977-03 DIN EN 49-1: 1992-11, 2005-06 EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 49-1 June 2016 ICS 71.100.50 Supersedes EN 49-1:2005English Version Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival

9、 - Part 1: Application by surface treatment (Laboratory method) Produits de prservation du bois - Dtermination de lefficacit protectrice vis-vis dAnobium punctatum (De Geer) par lobservation de la ponte et du taux de survie des larves - Partie 1: Application par traitement de surface (Mthode de labo

10、ratoire) Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des berlebens von Larven - Teil 1: Oberflchenverfahren (Laboratoriumsverfahren) This European Standard was approved by CEN on 5 January 2017. CEN members are bound t

11、o comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-C

12、ENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has

13、the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxe

14、mbourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey andUnited Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brusse

15、ls 2016 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 49-1:2016 E2 Contents Page European foreword 4 Introduction . 5 1 Scope . 6 2 Normative references . 6 3 Terms and definitions 6 4 Principle 6 5 Test materials 7 5.1 Biologica

16、l material . 7 5.2 Products and reagents 7 5.3 Apparatus 8 6 Sampling 8 7 Test specimens 8 7.1 Species of wood . 8 7.2 Wood quality 9 7.3 Provision of test specimens 9 7.4 Dimensions of test specimens . 9 7.5 Number of test specimens . 9 8 Procedure. 10 8.1 Preparation of the test specimens 10 8.1.1

17、 Conditioning of test specimens prior to sealing 10 8.1.2 Sealing . 10 8.1.3 Treatment of the test specimens . 10 8.1.4 Drying and conditioning of the test specimens after treatment 11 8.2 Exposure of the test specimens to the insects 12 8.3 Conditions and duration of the test 12 8.4 Examination of

18、the test specimens . 12 9 Validity of test . 12 10 Expression of results 13 10.1 Assessment of the protective effectiveness . 13 10.2 Toxic values . 13 11 Test report . 13 Annex A (informative) Example of a test report . 15 Annex B (informative) Identification of sex of test insects Anobium punctatu

19、m 17 Annex C (informative) Culturing technique for Anobium punctatum . 18 C.1 Culture wood . 18 C.1.1 Wood species 18 C.1.2 Collection of culture wood . 18 C.1.3 Cutting of culture wood . 18 EN 49-1:2016 (E) DIN EN 49-1:2016-11 3 C.1.4 Drying of culture wood 18 C.2 Source of beetles 18 C.2.1 Collect

20、ion of beetles 18 C.2.2 Quarantine of beetles . 18 C.3 Infestation of culture wood 18 C.3.1 Culture vessels 18 C.3.2 Preparation of wood . 18 C.3.3 Introduction of beetles 19 C.4 Culturing conditions . 19 C.4.1 Normal environment 19 C.4.2 Natural pupation induction . 19 C.4.3 Artificial pupation ind

21、uction . 19 C.5 Collection of beetles 19 C.6 General culture hygiene 19 Annex D (informative) Environmental, health and safety precautions within chemical/biological laboratory 21 Bibliography 22 EN 49-1:2016 (E) DIN EN 49-1:2016-11 4 European foreword This document (EN 49-1:2016) has been prepared

22、by Technical Committee CEN/TC 38 “Durability of wood and wood-based products”, the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2016, and conflic

23、ting national standards shall be withdrawn at the latest by December 2016. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This docume

24、nt supersedes EN 49-1:2005. Significant technical differences between this document and EN 49-1:2005 are as follows: a) generalization of material for preparing the egg-laying zones; b) introduction of new harmonized specifications for wood quality. This document consists of two parts, Part 1 is req

25、uired to enable effectiveness assessments of wood preservatives that are intended to be applied by surface treatment and Part 2 those that are intended to be applied by impregnation. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries ar

26、e bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Po

27、rtugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. EN 49-1:2016 (E) DIN EN 49-1:2016-11 5 Introduction This document describes a laboratory method of testing which gives a basis for assessment of the effectiveness of a wood preservative, when applied as a

28、 surface treatment, against Anobium punctatum. It allows the determination of the concentration at which the product prevents the development of infestation from egg laying. It can also be used with formulations ready for use. The method simulates conditions that can occur in practice on timber whic

29、h has been treated some time previously with wood preservative applied by dip, brush or spray and on which eggs of Anobium punctatum are laid. This laboratory method provides one criterion by which the value of a product can be assessed. In making this assessment the methods by which the preservativ

30、e may be applied should be taken into account. It is further recommended that results from this test should be supplemented by those from other appropriate tests, and above all by comparison with practical experience. When products which are very active at low concentrations are used it is very impo

31、rtant to take suitable precautions to isolate and separate, as far as possible, operations involving chemical products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities, condition

32、ing chambers and special training for personnel (see also Annex D for environmental, health and safety precautions). EN 49-1:2016 (E) DIN EN 49-1:2016-11 6 1 Scope This European Standard specifies a method for the determination of the protective effectiveness or the toxic values of a wood preservati

33、ve against infestation by Anobium punctatum (De Geer) when the product is applied as a surface treatment to wood. This method is applicable to: water-insoluble chemicals that are being studied as active insecticides; organic formulations, as supplied or as prepared in the laboratory by dilution of c

34、oncentrates; organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; water-soluble materials, for example salts. NOTE This method may be used in conjunction with an ageing procedure, for example EN 73. 2 Normative references The following docu

35、ments, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN ISO 835, Laboratory gl

36、assware Graduated pipettes (ISO 835) EN ISO 3696, Water for analytical laboratory use Specification and test methods (ISO 3696) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample having its physical or chemical chara

37、cteristics identical to the volumetric average characteristics of the total volume being sampled SOURCE: EN 1001-2:2005, 4.71 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to be tested) Note 1 to entry: Adapted from EN 10012:2005, 4.83. 4 Principle Dep

38、ending on the test being carried out either: on a set of test specimens of a susceptible wood species that is surface treated with a solution of the preservative, or EN 49-1:2016 (E) DIN EN 49-1:2016-11 7 if toxic values are to be determined, on several sets of test specimens of a susceptible wood s

39、pecies that are surface treated with a series of solutions in which the concentration of preservative is ranged in a given progression. The treated test specimens are exposed to gravid females of Anobium punctatum. The number of eggs laid, the number of eggs hatched and the numbers of surviving larv

40、ae are observed and compared with those in untreated control test specimens. If the preservative has been prepared in the laboratory by dilution of a concentrate or by dissolution of a solid, the resulting attack is also compared to that in solvent or diluent treated control test specimens. 5 Test m

41、aterials 5.1 Biological material Anobium punctatum (De Geer) Adult males and females in good condition. Adults to be used in the test shall be collected at daily intervals from naturally infested wood or laboratory culture (see Annex C). Use recently emerged adults which have been recently collected

42、; kept overnight in quarantine (see C.6); and then checked to ensure that they are undamaged, active and free from any infestation by mites. Determine the sex (see Annex B) of the collected and checked adults and place the males and females in separate containers. NOTE The proportion of males and fe

43、males varies during the emergence period. 5.2 Products and reagents 5.2.1 Paraffin wax, for sealing the relevant faces of test specimens to be treated with solutions in which water is the continuous phase. NOTE Paraffin wax with a setting point of 52 C to 54 C has been found to be suitable. 5.2.2 Ge

44、latin, for sealing the relevant faces of test specimens to be treated with solutions in which an organic solvent is the continuous phase. 5.2.3 Paste, for securing filter paper. The paste shall be starch-free, non-toxic to Anobium punctatum and insoluble in the product under test. NOTE Sodium carbox

45、y methyl cellulose, food grade, has been found to be suitable. 5.2.4 Water, complying with grade 3 of EN ISO 3696. 5.2.5 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period t

46、hat has a toxic effect on the insects. CAUTION Do not use benzene or other solvents which pose a health risk. 5.2.6 Filter paper, ordinary quality, medium-fast grade. 5.2.7 Fine cloth of a suitable material with a mesh aperture of 0,3 mm to 0,6 mm for the preparation of the egg-laying zones. NOTE Co

47、tton, linen and polyamide-gauze have been proven as suitable. EN 49-1:2016 (E) DIN EN 49-1:2016-11 8 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, controlled at (21 2) C, and at relative humidity (80 5) %. 5.3.2 Conditioning chamber, well ventilated, controlled at (20 2) C and at rela

48、tive humidity (65 5) %. The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.4) provided that this has the conditions specified for the conditioning chamber (see 5.3.2). 5.3.3 Treatment vessel, of a material that does not react with the preservative under test,

49、for example of glass for organic products and of polyethylene for salts containing fluorine. 5.3.4 Laboratory work area, well ventilated, where treatment of the test specimens is carried out. CAUTION It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours. 5.3.5 Testing chamber, with conditions identical to those of the culturing ch