DIN EN ISO 10272-1-2017 Microbiology of the food chain - Horizontal method for detection and enumeration of Campylobacter spp - Part 1 Detection method (ISO 10272-1 2017) German ve.pdf

1、September 2017 English price group 15No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 07.100.30!%mPl“2744573www.din.

2、deDIN EN ISO 10272-1Microbiology of the food chain Horizontal method for detection and enumeration of Campylobacter spp. Part 1: Detection method (ISO 102721:2017);English version EN ISO 102721:2017,English translation of DIN EN ISO 10272-1:2017-09Mikrobiologie der Lebensmittelkette Horizontales Ver

3、fahren zum Nachweis und zur Zhlung von Campylobacter spp. Teil 1: Nachweisverfahren (ISO 102721:2017);Englische Fassung EN ISO 102721:2017,Englische bersetzung von DIN EN ISO 10272-1:2017-09Microbiologie de la chane alimentaire Mthode horizontale pour la recherche et le dnombrement de Campylobacter

4、spp. Partie 1: Mthode de recherche (ISO 102721:2017);Version anglaise EN ISO 102721:2017,Traduction anglaise de DIN EN ISO 10272-1:2017-09SupersedesDIN EN ISO 102721:200604www.beuth.deDocument comprises 34 pagesDTranslation by DIN-Sprachendienst.In case of doubt, the German-language original shall b

5、e considered authoritative.08.17 DIN EN ISO 10272-1:2017-09 2 A comma is used as the decimal marker. National foreword This document (EN ISO 10272-1:2017) has been prepared by Technical Committee ISO/TC 34 “Food products” in collaboration with Technical Committee CEN/TC 275 “Food analysis Horizontal

6、 methods” (Secretariat: DIN, Germany). The responsible German body involved in its preparation was DIN-Normenausschuss Lebensmittel und landwirtschaftliche Produkte (DIN Standards Committee Food and Agricultural Products), Working Committee NA 057-01-06 AA “Microbiology of the food chain”. The DIN S

7、tandards corresponding to the International Standards referred to in this document are as follows: ISO 6887 (all parts) DIN EN ISO 6887 (all parts) ISO 7218 DIN EN ISO 7218 ISO 11133 DIN EN ISO 11133 ISO 5725-2:1994 DIN ISO 5725-2:2002-12 ISO 13307 DIN EN ISO 13307 ISO 16140 (all parts) DIN EN ISO 1

8、6140 (all parts) ISO 17468 DIN EN ISO 17468 ISO 17604 DIN EN ISO 17604 ISO/TS 17728 DIN CEN ISO/TS 17728 (DIN SPEC 10053) ISO 18593 DIN ISO 18593 Amendments This standard differs from DIN EN ISO 10272-1:2006-04 as follows: a) samples from the primary production stage have been added to the scope; b)

9、 the detection method has been extended to include the option of a second enrichment broth (Preston broth), primarily to overcome problems with background flora resistant to third generation -lactams (like cefoperazone in Bolton broth); c) the detection method has been extended to include the option

10、 of direct plating on mCCDA; d) the note on the use of closed containers with reduced headspace as an alternative to incubation in a microaerobic atmosphere has been deleted; e) the confirmation tests on study of microaerobic growth at 25 C and aerobic growth at 41,5 C have been replaced by the stud

11、y of aerobic growth at 25 C; f) performance testing for the quality assurance of the culture media has been added to Annex B; g) performance characteristics have been added to Annex C. Previous editions DIN EN ISO 10272-1: 2006-04 DIN EN ISO 10272-1:2017-09 3 National Annex NA (informative) Bibliogr

12、aphy DIN CEN ISO/TS 17728 (DIN SPEC 10053), Microbiology of the food chain Sampling techniques for microbiological analysis of food and feed samples DIN EN ISO 6887 (all parts), Microbiology of the food chain Preparation of test samples, initial suspension and decimal dilutions for microbiological e

13、xamination DIN EN ISO 7218, Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations DIN EN ISO 11133, Microbiology of food, animal feed and water Preparation, production, storage and performance testing of culture media DIN EN ISO 13307, Micr

14、obiology of food and animal feed Primary production stage Sampling techniques DIN EN ISO 16140 (all parts), Microbiology of the food chain Method validation Part 1: Vocabulary DIN EN ISO 17468, Microbiology of the food chain Technical requirements and guidance on establishment or revision of a stand

15、ardized reference method DIN EN ISO 17604, Microbiology of the food chain Carcass sampling for microbiological analysis DIN ISO 5725-2, Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard m

16、easurement method DIN ISO 18593, Microbiology of food and animal feeding stuffs Horizontal methods for sampling techniques from surfaces using contact plates and swabs DIN EN ISO 10272-1:2017-09 4 This page is intentionally blank EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 10272-1 July

17、2017 ICS 07.100.30 Supersedes EN ISO 10272-1:2006English Version Microbiology of the food chain - Horizontal method for detection and enumeration of Campylobacter spp. - Part 1: Detection method (ISO 10272-1:2017) Microbiologie de la chane alimentaire - Mthode horizontale pour la recherche et le dno

18、mbrement de Campylobacter spp. - Partie 1 : Mthode de recherche (ISO 10272-1:2017) Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zum Nachweis und zur Zhlung von Campylobacter spp. - Teil 1: Nachweisverfahren(ISO 10272-1:2017) This European Standard was approved by CEN on 1 May 2017. C

19、EN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on ap

20、plication to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC

21、Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, L

22、atvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre:

23、Avenue Marnix 17, B-1000 Brussels 2017 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 10272-1:2017 EEuropean foreword .4Introduction .61 Scope 72 Normative references . 73 Terms and definitions 74 Principle . 84.1 General 84.2

24、 Enrichment in selective liquid medium 84.2.1 Detection procedure A . 84.2.2 Detection procedure B . 84.2.3 Detection procedure C . 84.3 Isolation on selective solid medium 94.3.1 Detection procedure A . 94.3.2 Detection procedure B . 94.3.3 Detection procedure C . 94.3.4 Detection procedure A, B an

25、d C . 94.4 Confirmation 95 Culture media and reagents . 96 Equipment and consumables . 97 Sampling 108 Preparation of test sample . 109 Procedure. 109.1 General . 109.2 Test portion and initial suspension . 119.2.1 General 119.2.2 Detection procedure A 119.2.3 Detection procedure B 119.2.4 Detection

26、 procedure C 119.3 Enrichment . 129.3.1 Detection procedure A 129.3.2 Detection procedure B 129.4 Isolation 129.4.1 Detection procedure A 129.4.2 Detection procedure B 129.4.3 Detection procedures A, B and C 129.5 Confirmation of Campylobacter 129.5.1 General 129.5.2 Selection of colonies for confir

27、mation 139.5.3 Examination of morphology and motility 139.5.4 Study of aerobic growth at 25 C . 139.5.5 Detection of oxidase activity 139.5.6 Interpretation . 139.6 Identification of Campylobacter species (optional) 149.6.1 General 149.6.2 Detection of catalase activity. 149.6.3 Detection of hippura

28、te hydrolysis . 149.6.4 Detection of indoxyl acetate hydrolysis . 149.6.5 Interpretation . 15Contents PageDIN EN ISO 10272-1:2017-09 EN ISO 10272-1:2017 (E) 2Foreword .511.1 Interlaboratory study . 1511.2 Sensitivity . 1511.3 Specificity . 1511.4 LOD501512 Test report 16Annex A (normative) Diagram o

29、f procedures 17Annex B (normative) Culture media and reagents .18Annex C (informative) Method validation studies and performance characteristics .27Bibliography .30DIN EN ISO 10272-1:2017-09 EN ISO 10272-1:2017 (E) 310 Expression of results . 1511 Performance characteristics of the method 15European

30、 foreword This document (EN ISO 10272-1:2017) has been prepared by Technical Committee ISO/TC 34 “Food products” in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of which is held by DIN. This European Standard shall be given the status of a na

31、tional standard, either by publication of an identical text or by endorsement, at the latest by January 2018 and conflicting national standards shall be withdrawn at the latest by January 2018. Attention is drawn to the possibility that some of the elements of this document may be the subject of pat

32、ent rights. CEN shall not be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 10272-1:2006. This document has been prepared under a mandate given to CEN by the European Commission and the European Free Trade Association. According to the CEN-CENELEC Int

33、ernal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland,

34、Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 10272-1:2017 has been approved by CEN as EN ISO 10272-1:2017 without any mod

35、ification. DIN EN ISO 10272-1:2017-09 EN ISO 10272-1:2017 (E) 4 ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical com

36、mittees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the

37、 International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for th

38、e different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights.

39、 ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www .iso .org/ patents).Any trade name used in this

40、 document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the World Trade Organization (WTO) principles in

41、 the Technical Barriers to Trade (TBT) see the following URL: www .iso .org/ iso/ foreword .html.This document was prepared by the European Committee for Standardization (CEN), Technical Committee CEN/TC 275, Food Analysis Horizontal methods, in collaboration with ISO Technical committee ISO/TC 34,

42、Food products, Subcommittee SC 9, Microbiology, in accordance with the agreement on technical cooperation between ISO and CEN (Vienna Agreement).This second edition cancels and replaces the first edition (ISO 10272-1:2006), which has been technically revised with the following main changes: samples

43、from the primary production stage have been added to the scope; the detection method was extended to include the option of a second enrichment broth (Preston broth), primarily to overcome problems with background flora resistant to third generation -lactams (like cefoperazone in Bolton broth); the d

44、etection method was extended to include the option of direct plating on mCCDA; the note on the use of closed containers with reduced headspace as an alternative to incubation in a microaerobic atmosphere has been deleted; the confirmation tests on study of microaerobic growth at 25 C and aerobic gro

45、wth at 41,5 C were replaced by the study of aerobic growth at 25 C; performance testing for the quality assurance of the culture media has been added to Annex B; performance characteristics have been added to Annex C.A list of all parts in the ISO 10272 series can be found on the ISO website.DIN EN

46、ISO 10272-1:2017-09 EN ISO 10272-1:2017 (E) 5 IntroductionThe main changes, listed in the foreword, introduced in this document compared to ISO 10272-1:2006 are considered as minor (see ISO 17468).Because of the large variety of food and feed products, this horizontal method may not be appropriate i

47、n every detail for certain products, and for some other products it may be necessary to use different methods. Nevertheless, it is hoped that in all cases every attempt will be made to apply this horizontal method as far as possible and that deviations from this will only be made if absolutely neces

48、sary for technical reasons.When this document is next reviewed, account will be taken of all information then available regarding the extent to which this horizontal method has been followed, and the reasons for deviations from this in the case of particular products. The harmonization of test methods cannot be immediate and, for certain group of products, International Standards and/or national standards may already exist that do not comply with this horizontal method. It is hoped that when such standards are reviewed, they will be changed to comply with this document so