DIN EN ISO 11269-1-2013 Soil quality - Determination of the effects of pollutants on soil flora - Part 1 Method for the measurement of inhibition of root growth (ISO 11269-1 2012) .pdf

1、March 2013 Translation by DIN-Sprachendienst.English price group 13No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS

2、13.080.30!$or“1957679www.din.deDDIN EN ISO 11269-1Soil quality Determination of the effects of pollutants on soil flora Part 1: Method for the measurement of inhibition of root growth(ISO 11269-1:2012);English version EN ISO 11269-1:2012,English translation of DIN EN ISO 11269-1:2013-03Bodenbeschaff

3、enheit Bestimmung der Wirkungen von Schadstoffen auf die Bodenflora Teil 1: Verfahren zur Messung der Wurzelwachstumshemmmung (ISO 11269-1:2012);Englische Fassung EN ISO 11269-1:2012,Englische bersetzung von DIN EN ISO 11269-1:2013-03Qualit du sol Dtermination des effets des polluants sur la flore d

4、u sol Partie 1: Mthode de mesurage de linhibition de la croissance des racines(ISO 11269-1:2012);Version anglaise EN ISO 11269-1:2012,Traduction anglaise de DIN EN ISO 11269-1:2013-03SupersedesDIN ISO 11269-1:1997-06www.beuth.deDocument comprises 2 pagesIn case of doubt, the German-language original

5、 shall be considered authoritative.402.13DIN EN ISO 11269-1:2013-03 2 A comma is used as the decimal marker. National foreword The text of ISO 11269-1:2012 has been prepared by Technical Committee ISO/TC 190 “Soil quality” and has been taken over as EN ISO 11269-1:2012 by Technical Committee CEN/TC

6、345 “Characterization of soils” (Secretariat: NEN, Netherlands). The responsible German body involved in its preparation was the Normenausschuss Wasserwesen (Water Practice Standards Committee), Working Committee NA 119-01-02-04 UA Biologische Verfahren of NA 119-01-02 AA Abfall- und Bodenuntersuchu

7、ng. ISO 11269 consists of the following parts, under the general title Soil quality Determination of the effects of pollutants on soil flora: Part 1: Method for the measurement of inhibition of root growth Part 2: Effects of contaminated soil on the emergence and early growth of higher plants Expert

8、 assistance and specialized laboratories will be required to perform the analysis described in this standard. The DIN Standards corresponding to the International Standards referred to in this document are as follows: ISO 10381-6 DIN ISO 10381-6 ISO 10390 DIN ISO 10390 ISO 10694 DIN ISO 10694 ISO 10

9、930 E DIN EN ISO 10930 ISO 11260 DIN EN ISO 11260 ISO 11268-1 DIN ISO 11268-1 ISO 11268-2 DIN ISO 11268-2 ISO 11277 DIN ISO 11277 ISO 11465 DIN ISO 11465 Amendments This standard differs from DIN ISO 11269-1:1997-06 as follows: a) ISO 11269-1 has been taken over as European Standard EN ISO 11269-1;

10、b) the scope has been extended to include compost and sludge; c) criteria allowing validation of the test results have been added; d) Annex B (former Annex D) has been modified with regard to the data given for the reference substance; e) the limits of the method for some soil types have been indica

11、ted. Previous editions DIN ISO 11269-1: 1997-06 DIN EN ISO 11269-1:2013-03 3 National Annex NA (informative) Bibliography E DIN EN ISO 10930, Soil quality Measurement of the stability of soil aggregates subjected to the action of water DIN ISO 11260, Soil quality Determination of effective cation ex

12、change capacity and base saturation level using barium chloride solution DIN ISO 10381-6, Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory DIN ISO 1

13、0390, Soil quality Determination of pH DIN ISO 10694, Soil quality Determination of organic and total carbon after dry combustion (elementary analysis) DIN ISO 11268-1, Soil quality Effects of pollutants on earthworms (Eisenia fetida) Part 1: Determination of acute toxicity using artificial soil sub

14、strate DIN ISO 11268-2, Soil quality Effects of pollutants on earthworms (Eisenia Fetida) Part 2: Determination of effects on reproduction DIN ISO 11277, Soil quality Determination of particle size distribution in mineral soil material Method by sieving and sedimentation DIN ISO 11465, Soil quality

15、Determination of dry matter and water content on a mass basis Gravimetric method DIN EN ISO 11269-1:2013-03 4 This page is intentionally blank EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 11269-1 December 2012 ICS 13.080.30 English Version Soil quality - Determination of the effects of p

16、ollutants on soil flora - Part 1: Method for the measurement of inhibition of root growth (ISO 11269-1:2012) Qualit du sol - Dtermination des effets des polluants sur la flore du sol - Partie 1: Mthode de mesurage de linhibition de la croissance des racines (ISO 11269-1:2012)Bodenbeschaffenheit - Be

17、stimmung der Wirkungen von Schadstoffen auf die Bodenflora - Teil 1: Verfahren zur Messung der Wurzelwachstumshemmmung (ISO 11269-1:2012) This European Standard was approved by CEN on 11 November 2012. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the cond

18、itions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exist

19、s in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national stan

20、dards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Sl

21、ovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2012 CEN All rights of exploitation in any form and by any means reserved worldwide

22、 for CEN national Members. Ref. No. EN ISO 11269-1:2012: EContents PageForeword Introduction 1 Scope 2 Normative references .3 Terms and definitions .4 Principle .5 Test plants .6 Materials .6.1 Test vessels 6.2 Soil .7 Equipment .8 Reference substance 9 Procedure 9.1 Experimental design .9.2 Prepar

23、ation of pots 9.3 Pregermination of the seeds 9.4 Growing conditions 9.5 Test duration .9.6 Measurements 10 Expression of results and data 11 Validity criteria .12 Test report .Annex A (informative) Recommended method for measuring the water-holding capacity of the soil.Annex B (informative) Results

24、 of tests performed on reference substances 14Annex C (informative) Example of results obtained with boric acid using sand as the substrate 15Annex D (informative) Recommended methods for the incorporation of chemicals into soils 16Annex E (informative) Recommended methods for the incorporation of c

25、ompost, sludge or waste into soils 17Annex F (informative) Example of seedlings of winter barley collected at the end of the test after removal from artificial soil 19Bibliography .DIN EN ISO 11269-1:2013-03 EN ISO 11269-1:2012 (E) 23455567777999910101010111111111320Foreword The text of ISO 11269-1:

26、2012 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the International Organization for Standardization (ISO) and has been taken over as EN ISO 11269-1:2012 by Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN. This European Stan

27、dard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by June 2013, and conflicting national standards shall be withdrawn at the latest by June 2013. Attention is drawn to the possibility that some of the elements of this d

28、ocument may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. According to the CEN/CENELEC Internal Regulations, the national standards organisations of the following countries are bound to implement this European Standar

29、d: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain

30、, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 11269-1:2012 has been approved by CEN as a EN ISO 11269-1:2012 without any modification. DIN EN ISO 11269-1:2013-03 EN ISO 11269-1:2012 (E) 3 IntroductionChemical analysis of soil samples or waste materials to b

31、e disposed on soil, together with ecotoxicological testing, provides substantial evidence of the suitability of the soil for arable production, or gives information on the potential environmental risk resulting from the disposal of wastes such as sewage sludge on farmland. There is also a need to as

32、sess the quality of the soil after reclamation of industrial sites and colliery tips or when capping landfill sites. As the ability of the soil to grow crops is the main criterion, a rapid-growth test has been developed, based on seedling growth in controlled environmental conditions.Two major prere

33、quisites of a phytotoxicity test are that it provides consistently reliable results and that it can be used at any time of the year. It is therefore essential that seeds be grown in a controlled environment to ensure optimal growing conditions which can be maintained for any number of tests, produci

34、ng reproducible results over a long period of time.The test method described in this part of ISO 11269 can be used to compare soils, to monitor changes in their activity or to determine the effect of added chemicals or materials (compost, sludge, waste).DIN EN ISO 11269-1:2013-03 EN ISO 11269-1:2012

35、 (E) 41 ScopeThis part of ISO 11269 describes a method for the determination of the effects of contaminated soils or contaminated samples on the root elongation of terrestrial plants.This method is applicable to soils, soil materials, compost, sludge, waste or chemical testing. It is applicable to t

36、he comparison of soils of known and unknown quality and to the measurement of effects of materials (compost, sludge, waste) or chemicals deliberately added to the soil.The method is not intended to be used as a measure of the ability of the soil to support sustained plant growth.2 Normative referenc

37、esThe following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO 10381-6, Soil quality Sampling Part 6: Guida

38、nce on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratoryISO 10390, Soil quality Determination of pHISO 10694, Soil quality Determination of organic and total carbon after dry combustion (elem

39、entary analysis)ISO 10930, Soil quality Measurement of the stability of soil aggregates subjected to the action of waterISO 11260, Soil Quality Determination of effective cation exchange capacity and base saturation level using barium chloride solutionISO 11268-1, Soil quality Effects of pollutants

40、on earthworms Part 1: Determination of acute toxicity to Eisenia fetida/Eisenia andreiISO 11268-2, Soil quality Effects of pollutants on earthworms Part 2: Determination of effects on reproduction to Eisenia fetida/Eisenia andreiISO 11277, Soil quality Determination of particle size distribution in

41、mineral soil material Method by sieving and sedimentationISO 11465, Soil quality Determination of dry matter and water content on a mass basis Gravimetric methodISO/TS 20281, Water quality Guidance on statistical interpretation of ecotoxicity data3 Terms and definitionsFor the purposes of this docum

42、ent, the following terms and definitions apply.3.1contaminantsubstance or agent present in the soil as a result of human activityDIN EN ISO 11269-1:2013-03 EN ISO 11269-1:2012 (E) 5 3.2test mixturemixture of test soil or test material (compost, sludge, waste or chemical) with control soil3.3radiclep

43、ortion of the plant embryo which develops into the primary root3.4hypocotylportion of the axis of an embryo or seedling situated between the cotyledons (seed leaves) and the radicle3.5reference soiluncontaminated site-specific soil (e.g. collected in the vicinity of a contaminated site) with similar

44、 properties (nutrient concentrations, pH, organic carbon content and texture) as the test soil3.6standard soilfield-collected soil or artificial soil whose main properties (e.g. pH, texture, organic matter content) are within a known rangeEXAMPLE Euro-soils1, artificial soil2, LUFA soil.1)NOTE The p

45、roperties of standard soils can differ from the test soil.3.7control soilreference or standard soil used as a control and as a medium for preparing dilution series with test soil or test material (e.g. compost, sludge, waste, chemical)NOTE Both the effective concentration (ECx) and the no-observed-e

46、ffect concentration (NOEC) are expressed in milligrams of test substance per kilogram (dry mass) of the test substrate. Soil mixtures are given in percent based on soil dry mass.3.8effective concentrationECxeffective concentration (dilution) of the test soil or test material (e.g. compost, sludge, w

47、aste, chemical) at which root elongation is reduced by x % compared to the control4 PrincipleThis method compares the root elongation of terrestrial plants in a test soil and/or a series of dilutions with a control soil. This method may also be used for the testing of compost, sludge, waste or chemi

48、cals by applying various concentrations of the material under investigation to a control soil.Pregerminated seeds are exposed to the test material under controlled conditions. After the growth period, the lengths of the roots of the test plants are compared with those of the control plants. Statisti

49、cally significant differences in the root lengths of seedlings grown in any test medium compared to the controls are indicative of an effect.NOTE Shoot height is also a useful parameter, and this can be measured in conjunction with root length to provide additional or corroborative data.1) Euro-soils, artificial soil and LUFA so