DIN EN ISO 17075-2-2017 Leather - Chemical determination of chromium(VI) content in leather - Part 2 Chromatographic method (ISO 17075-2 2017) German version EN ISO 17075-2 2017《皮革.pdf

1、May 2017 English price group 12No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 59.140.30!%eBr“2663179www.din.deDIN

2、EN ISO 17075-2Leather Chemical determination of chromium(VI) content in leather Part 2: Chromatographic method (ISO 170752:2017);English version EN ISO 170752:2017,English translation of DIN EN ISO 17075-2:2017-05Leder Chemische Bestimmung des Chrom(VI)Gehalts in Leder Teil 2: Chromatographie (ISO 1

3、70752:2017);Englische Fassung EN ISO 170752:2017,Englische bersetzung von DIN EN ISO 17075-2:2017-05Cuir Dtermination chimique de la teneur en chrome(VI) du cuir Partie 2: Mthode chromatographique (ISO 170752:2017);Version anglaise EN ISO 170752:2017,Traduction anglaise de DIN EN ISO 17075-2:2017-05

4、Together with DIN EN ISO 170751:201705,supersedesDIN EN ISO 17075:200802www.beuth.deDocument comprises 22 pagesDTranslation by DIN-Sprachendienst.In case of doubt, the German-language original shall be considered authoritative.05.17 DIN EN ISO 17075-2:2017-05 2 A comma is used as the decimal marker.

5、 National foreword This document (EN ISO 17075-2:2017) has been prepared by Technical Committee IULTCS “International Union of Leather Technologists and Chemists Societies” in collaboration with Technical Committee CEN/TC 289 “Leather” (Secretariat: UNI, Italy). The responsible German body involved

6、in its preparation was DIN-Normenausschuss Materialprfung (DIN Standards Committee Materials Testing), Working Committee NA 062-05-52 AA “Chemical test methods of leather”. The DIN Standards corresponding to the International Standards referred to in this document are as follows: ISO 2418 DIN EN ISO

7、 2418 ISO 3696 DIN ISO 3696 ISO 4684 DIN EN ISO 4684 Amendments This standard differs from DIN EN ISO 17075:2008-02 as follows: a) the standard has been subdivided into Part 1, colorimetric method and Part 2, chromatography; b) in 7.1, the sample preparation has been revised; c) 7.1 and 7.2 have bee

8、n technically revised; d) the standard has been editorially revised. Previous editions DIN 53314: 1996-04 DIN CEN/TS 14495: 2003-08 DIN EN ISO 17075: 2008-02 National Annex NA (informative) Bibliography DIN EN ISO 2418, Leather Chemical, physical and mechanical and fastness tests Sampling location D

9、IN EN ISO 4684, Leather Chemical tests Determination of volatile matter DIN ISO 3696, Water for analytical laboratory use Specification and test methods EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 17075-2 February 2017 ICS 59.140.30 Supersedes EN ISO 17075:2007English Version Leather -

10、Chemical determination of chromium(VI) content in leather - Part 2: Chromatographic method(ISO 17075-2:2017) Cuir - Dtermination chimique de la teneur en chrome(VI) du cuir - Partie 2: Mthode chromatographique (ISO 17075-2:2017) Leder - Chemische Bestimmung des Chrom(VI)-Gehalts in Leder - Teil 2: C

11、hromatographie (ISO 17075-2:2017)This European Standard was approved by CEN on 30 December 2016. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-

12、date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translati

13、on under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, F

14、ormer Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZA

15、TION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2017 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 17075-2:2017 EEuropean foreword .31 Scope

16、. 52 Normative references 53 Terms and definitions . 54 Principle 65 Chemicals . 66 Apparatus and materials 67 Procedure. 77.1 Sampling and preparation of samples 77.2 Preparation of analytical solution 77.3 Chromatographic conditions 87.4 Calibration 87.5 Determination of the recovery rate . 98 Cal

17、culation and expression of results . 98.1 Calculation of chromium(VI) content 98.2 Recovery rate (according to 7.5) 108.3 Expression of results . 109 Test report 10Annex A (informative) Accuracy . 11Annex B (informative) Chromatographic conditions for direct detection method . 13Annex C (informative

18、) Chromatographic conditions for method with post-column reaction.16Annex D (informative) Comparison between the colorimetric method (ISO 17075-1) and the ionic chromatography method (ISO 17075-2) .20Contents PageDIN EN ISO 17075-2:2017-05 EN ISO 17075-2:2017 (E) 2Foreword .4European foreword This d

19、ocument (EN ISO 17075-2:2017) has been prepared by Technical Committee IULTCS “International Union of Leather Technologists and Chemists Societies“ in collaboration with Technical Committee CEN/TC 289 “Leather” the secretariat of which is held by UNI. This European Standard shall be given the status

20、 of a national standard, either by publication of an identical text or by endorsement, at the latest by August 2017 and conflicting national standards shall be withdrawn at the latest by August 2017. Attention is drawn to the possibility that some of the elements of this document may be the subject

21、of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 17075:2007. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this

22、 European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia,

23、Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 17075-2:2017 has been approved by CEN as EN ISO 17075-2:2017 without any modification. DIN EN ISO 17075-2:2017-05 EN ISO 17075-2:2017 (E) 3 ForewordISO (the International Organization fo

24、r Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the

25、 right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The proced

26、ures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial

27、 rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights

28、 identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www .iso .org/ patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an expl

29、anation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: www .iso .org/ iso/ foreword .htmlISO 17075-2 wa

30、s prepared by the Chemical Test Commission of the International Union of Leather Technologists and Chemists Societies (IUC Commission, IULTCS) in collaboration with the European Committee for Standardization (CEN) Technical Committee CEN/TC 289, Leather, the secretariat of which is held by UNI, in a

31、ccordance with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).IULTCS, originally formed in 1897, is a world-wide organization of professional leather societies to further the advancement of leather science and technology. IULTCS has three Commissions, which are respons

32、ible for establishing international methods for the sampling and testing of leather. ISO recognizes IULTCS as an international standardizing body for the preparation of test methods for leather.This first edition of ISO 17075-2, together with ISO 17075-1, cancels and replaces ISO 17075:2007, which h

33、as been technically revised.The main changes compared to ISO 17075:2007 are as follows: the chromatographic analytical technique has been added; the sample preparation has been revised; mechanical shaking in 7.1 and 7.2 has been revised.A list of all parts in the ISO 17075 series can be found on the

34、 ISO website.DIN EN ISO 17075-2:2017-05 EN ISO 17075-2:2017 (E) 4 1 ScopeThis document specifies a method for determining chromium(VI) in solutions leached from leather under defined conditions. The method described is suitable to quantify the chromium(VI) content in leathers down to 3 mg/kg.This do

35、cument is applicable to all leather types.The results obtained from this method are strictly dependent on the extraction conditions. Results obtained by using other extraction procedures (extraction solution, pH, extraction time, etc.) are not comparable with the results produced by the procedure de

36、scribed in this document.If a leather sample is tested with both ISO 17075-1 and this document, the results obtained with this document are considered as the reference. The advantage of the method described in this document is that there are no interferences from the colour of the extract. Neverthel

37、ess, interlaboratory trials do not show significant differences (see Annex D) and the results are comparable between both methods.2 Normative referencesThe following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For d

38、ated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO 2418, Leather Chemical, physical and mechanical and fastness tests Sampling locationISO 3696, Water for analytical laboratory use Specificatio

39、n and test methodsISO 4044:2017, Leather Chemical tests Preparation of chemical test samplesISO 4684, Leather Chemical tests Determination of volatile matter3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.ISO and IEC maintain terminological databas

40、es for use in standardization at the following addresses: ISO Online browsing platform: available at h t t p :/ www .iso .org/ obp IEC Electropedia: available at h t t p :/ www .electropedia .org/ 3.1chromium(VI) contentamount of chromium(VI) in leather determined after extraction with an aqueous sa

41、lt solution at pH 7,0 to 8,0Note 1 to entry: The chromium(VI) content is reported as chromium(VI) in milligrams per kilogram (mg/kg), expressed as the dry mass of the sample.DIN EN ISO 17075-2:2017-05 EN ISO 17075-2:2017 (E) 5 4 PrincipleExtractable chromium(VI) is leached from the sample in phospha

42、te buffer at pH 7,0 to 8,0. An aliquot of the filtered extract is analysed for Cr(VI) using ion-exchange chromatography with UV-VIS detection.5 ChemicalsAll reagents used shall have at least analytical grade purity.5.1 Extraction solutionDissolve 22,8 g dipotassium hydrogenphosphate K2HPO43H2O in 1

43、000 ml water (5.7), adjusted to pH 8,0 0,1 with phosphoric acid (5.2). Degas this solution with either argon or nitrogen (5.6) or ultrasonic bath.Standard practice is to make up a fresh solution each day. However, the solution can be kept for up to one week in a refrigerator at (4 3) C but shall be

44、warmed to room temperature and degassed prior to use.5.2 Phosphoric acid solution700 ml o-phosphoric acid, = 1,71 g/ml, made up to 1 000 ml with water (5.7).First add approximately 200 ml of deionised water (5.7) to a 1 000 ml volumetric flask, then add the 700 ml of o-phosphoric acid and dilute to

45、the mark with deionised water.5.3 Potassium dichromate (K2Cr2O7), dried for (16 2) h at (102 2) C.5.4 Chromium(VI) stock solutionDissolve 2,829 g potassium dichromate (K2Cr2O7) (5.3) in water (5.7) in a volumetric flask and make up to 1 000 ml with water (5.7). One millilitre of this solution contai

46、ns 1 mg of chromium.A stock solution at this concentration level of hexavalent chromium is an alternative available commercially.5.5 Chromium(VI) standard solutionPipette 1 ml of solution (5.4) into a 1 000 ml volumetric flask and make up to the mark with extraction solution (5.1). One millilitre of

47、 this solution contains 1 g of chromium.The solution can be kept for up to one week in a refrigerator at (4 3) C but shall be warmed to room temperature prior to use.A stock solution of hexavalent chromium at this concentration level is an alternative available commercially.5.6 Argon or nitrogen, ox

48、ygen-freePreference should be given to argon as an inert gas instead of nitrogen because argon has a higher specific mass than air.5.7 Distilled or deionised water, Grade 3 quality as specified in ISO 3696.6 Apparatus and materialsUsual laboratory equipment and, in particular, the following.DIN EN ISO 17075-2:2017-05 EN ISO 17075-2:2017 (E) 6 6.1 Suitable mechanical orbital shaker, (100 10) min1.6.2 Conical flask,