1、February 2016 English price group 10No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 37.020!%LFj“2413571www.din.deDI
2、N ISO 9022-11Optics and photonics Environmental test methods Part 11: Mould growth (ISO 9022-11:2015),English translation of DIN ISO 9022-11:2016-02Optik und Photonik Umweltprfverfahren Teil 11: Schimmelwachstum (ISO 9022-11:2015),Englische bersetzung von DIN ISO 9022-11:2016-02Optique et photonique
3、 Mthodes dessais denvironnement Partie 11: Moississures (ISO 9022-11:2015),Traduction anglaise de DIN ISO 9022-11:2016-02SupersedesDIN ISO 9022-11:2000-09www.beuth.deDocument comprises 14 pagesDTranslation by DIN-Sprachendienst.In case of doubt, the German-language original shall be considered autho
4、ritative.01.16A comma is used as the decimal marker. Contents Page National foreword 3Introduction 51 Scope 62 Normative references 63 General information and test conditions 63.1 Test fungi 63.2 Fungal spore suspension 73.2.1 Fungal cultures 73.2.2 Spore suspensions 73.2.3 Mixed spore suspension 83
5、3 Control strips 83.4 Specimen 93.5 Incubation cabinets and climatic chambers . 104 Conditioning method 85: Mould growth 105 Procedure.105.1 General .105.2 Preconditioning 115.3 Activities during conditioning .115.4 Recovery .115.5 Evaluation 115.6 General level for acceptance 116 Environmental tes
6、t code .127 Specification 12Annex A (informative) Explanatory notes .13National Annex NA (informative) Bibliography. 4DIN ISO 9022-11:2016-02 2National foreword This document (ISO 9022-11:2015) has been prepared by Technical Committee ISO/TC 172 “Optics and photonics”, Subcommittee SC 1 “Fundamental
7、 standards” (Secretariat: DIN, Germany). The responsible German body involved in its preparation was DIN-Normenausschuss Feinmechanik und Optik (DIN Standards Committee Optics and Precision Mechanics), Working Committee NA 027-01-02 AA Grundnormen fr die Optik, Working Group NA 027-01-02-03 AK Umwel
8、tbedingungen und -prfungen fr optische Gerte. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. DIN and/or DKE shall not be held responsible for identifying any or all such patent rights. DIN ISO 9022 consists of the following parts
9、 under the general title Optics and photonics Environmental test methods: null Part 1: Definitions, extent of testing null Part 2: Cold, heat and humidity null Part 3: Mechanical stress null Part 4: Salt mist null Part 6: Dust null Part 7: Resistance to drip or rain null Part 8: High internal press
10、ure, low internal pressure, immersion null Part 9: Solar radiation and weathering null Part 11: Mould growth null Part 12: Contamination null Part 14: Dew, hoarfrost, ice null Part 17: Combined contamination, solar radiation null Part 20: Humid atmosphere containing sulfur dioxide or hydrogen sulfid
11、e null Part 22: Combined cold, dry heat or temperature change with bump or random vibration null Part 23: Low pressure combined with cold, ambient temperature and dry and damp heat The DIN Standard corresponding to the International Standard referred to in this document is as follows: ISO 9022-1 DIN
12、 ISO 9022-1 Amendments This standard differs from DIN ISO 9022-11:2000-09 as follows: a) normative references have been updated; b) the standard has been editorially updated; c) the text of International Standard ISO 9022-11:2015 has been adopted in its entirety. DIN ISO 9022-11:2016-02 3Previous ed
13、itions DIN 58390-11: 1985-06 DIN ISO 9022-11: 2000-09 National Annex NA (informative) Bibliography DIN ISO 9022-1, Optics and photonics Environmental test methods Part 1: Definitions, extent of testing DIN ISO 9022-11:2016-02 4IntroductionOptical instruments are affected during their use by a number
14、 of different environmental parameters which they are required to resist without significant reduction in performance and to remain within defined specifications.The type and severity of these parameters depend on the conditions of use of the instrument (for example, in the laboratory or workshop) a
15、nd on its geographical location. The environmental effects on optical instrument performance in the tropics and subtropics are totally different from those found when they are used in arctic regions. Individual parameters cause a variety of different and overlapping effects on instrument performance
16、The manufacturer attempts to ensure, and the user naturally expects, that instruments will resist the likely rigours of their environment throughout their life. This expectation can be assessed by exposure of the instrument to a range of simulated environmental parameters under controlled laborator
17、y conditions. The severity of these conditions is often increased to obtain meaningful results in a relatively short period of time.In order to allow assessment and comparison of the response of optical instruments to appropriate environmental conditions, ISO 9022 contains details of a number of lab
18、oratory tests which reliably simulate a variety of different environments. The tests are based largely on IEC standards, modified where necessary to take into account features special to optical instruments.As a result of continuous progress in all fields, optical instruments are no longer only prec
19、ision-engineered optical products, but, depending on their range of application, also contain additional assemblies from other fields. For this reason, the principal function of the instrument is to be assessed to determine which International Standard should be used for testing. If the optical func
20、tion is of primary importance, then ISO 9022 is applicable, but if other functions take precedence then the appropriate International Standard in the field concerned should be applied. Cases can arise where application of both ISO 9022 and other appropriate International Standards will be necessary.
21、Optics and photonics Environmental test methods Part 11: Mould growthDIN ISO 9022-11:2016-02 5WARNING Although the species of fungi selected for testing do not normally present a hazard to humans, certain people can develop allergies or other reactions. The use of experienced and trained personnel i
22、s required to ensure the proper handling of fungi and the appropriate conduct of the tests. It is, therefore, recommended to entrust the performance of the tests required by this part of ISO 9022 to a microbiological laboratory, since such laboratories have the appropriate equipment and trained pers
23、onnel.1 ScopeThis part of ISO 9022 specifies the methods relating to the environmental tests of optical instruments, including additional assemblies from other fields (e.g. mechanical, chemical, and electronic devices) under equivalent conditions, for their ability to resist the influence of mould g
24、rowth.However, complete instruments or assemblies are only tested as specified in this part of ISO 9022 in exceptional cases. Normally, representative specimens such as mounted optics, material samples, or surface coatings on representative samples are used for testing.The tests described in this pa
25、rt of ISO 9022 are designed for the selection of materials and components for instruments likely to be used in an environment that is conducive to mould growth, rather than for regular production control.The purpose of testing is to investigate to what extent the optical, climatic, mechanical, chemi
26、cal and electrical (including electrostatic) performance characteristics of the specimen are affected by mould growth.In addition, the tests in this part of ISO 9022 are designed to assess to what extent metabolic waste products (such as enzymes or acids) excreted by fungi, cause etching, corrosion,
27、 or short-circuits on, for instance, printed circuit boards.2 Normative referencesThe following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the lat
28、est edition of the referenced document (including any amendments) applies.ISO 9022-1, Optics and photonics Environmental test methods Part 1: Definitions, extent of testing3 General information and test conditions3.1 Test fungiThe species of fungi selected for testing (see Table 1) are frequently fo
29、und on optical glass surfaces. Among them are species of hydrophyl, mesophyl, and xerophyl fungi.DIN ISO 9022-11:2016-02 6Table 1 Test fungiSeries No. Species12345678910Aspergillus nigerAspergillus flavusAspergillus versicolorTrichoderma viridePenicillium funiculosumPenicillium citrinumPaecilomycesC
30、haetomium globosumEurotium tonophilumAspergillus penicilloiden (Vitrocolae)Since strains of fungi change their characteristics with time, only fungal species are specified. The test report or relevant specification, respectively, shall, however, specify the fungal strains used for testing.3.2 Fungal
31、 spore suspension3.2.1 Fungal culturesPure cultures of each of the fungus species specified in Table 1 shall be maintained separately on an appropriate agar medium (e.g. malt agar).The fungal cultures used for the spore suspension shall not be older than 14 days to 21 days and shall not be used more
32、 than once for preparing a mixed spore suspension.3.2.2 Spore suspensionsFor preparing the spore suspensions, and wherever else in this Subclause “water” is specified, use distilled or fully demineralized sterile water containing 0,05 % (mass fraction) of a non-toxic wetting agent such as sodium dio
33、ctylsulfosuccinate or sodium laurylsulfate.Pour 10 ml of the water into each of the fungal cultures described in 3.2.1.Using a sterile platinum loop or any other suitable means, carefully scrape the spores from the mycelial mat. Take care to leave out clumps of agar. Pour the spores charge into a st
34、erile Erlenmeyer flask containing 45 ml of water. Add sterile solid glass beads and shake vigorously to liberate the spores from the fruiting body and to break the spore clumps. Filter the dispersed fungal spore suspension through sterile glass wool to remove mycelial fragments.Centrifuge the filtra
35、te and discard the supernatant liquid. Resuspend the residue in 50 ml of water and centrifuge. Wash the spores obtained from each of the fungi in this manner three times.Dilute the final washed residue with the mineral salts solution specified in Table 2 in such a manner that each resultant spore su
36、spension contains (1 000 000 200 000) spores per millilitre, measured using a suitable counting chamber.DIN ISO 9022-11:2016-02 7Table 2 Mineral salts solutionComponentMassgPotassium dihydrogen orthophosphate (KH2PO4) 0,7Potassium monohydrogen orthophosphate (K2HPO4) 0,7Magnesium sulfate heptahydrat
37、e (MgSO4.7H2O) 0,7Ammonium nitrate (NH4NO3) 1,0Sodium chloride (NaCl) 0,005Iron(II) sulfate heptahydrate (FeSO4.7H2O) 0,002Zinc sulfate heptahydrate (ZnSO4.7H2O) 0,002Manganese(II) sulfate monohydrate (MnSO4.H2O) 0,001Distilled water (H2O) 1 000,0Sterilize the mineral salts solution in an autoclave
38、at 120 C for 20 min. Using sodium hydroxide solution, c(NaOH) = 0,01 mol/l, adjust the pH of the solution to 6,0 to 6,5 after sterilization. (Percentage purity of the chemicals: atomic adsorption spectroscopy.)Inoculate each of 10 Petri dishes containing an appropriate agar medium (e.g. malt agar) w
39、ith spore suspension and immediately incubate the dishes, to check the viability of each fungus species, in the incubation chamber to be used for exposing the specimens. In the event that fungicide-treated specimens are under test in the incubation chamber, expose the Petri dishes to exactly the sam
40、e climatic conditions in a separate incubation chamber. The absence of growth of any of the various fungus species, at the end of one week, will invalidate the results of all simultaneously performed tests using these spores. Such invalidated tests shall be repeated using freshly prepared mixed spor
41、e suspensions from new cultures.3.2.3 Mixed spore suspensionAfter having taken the inoculum from the spore suspensions for the purpose described in 3.2.2, blend equal portions of the 10 spore suspensions to obtain the final mixed spore suspension.The spore suspensions from the individual cultures as
42、 well as the mixed spore suspension shall be used on the day of their preparation. On no account shall they be stored for later use.3.3 Control stripsAlong with the specimens place at least three control strips in the exposure chamber in order to ensure that optimal climatic conditions are present i
43、n the incubation or climatic chamber during exposure of the contaminated specimens. The control strips are of no use if the specimens have been previously treated with fungicides; since these become active predominantly during the volatile phase, a fungicidal atmosphere would develop within the test
44、 chamber and hamper the fungal growth on the control strips. In such cases, only the separately incubated individual fungal cultures can be used as control.The control strips shall be of white sterilized filter paper and shall be of the same size as the specimen (see 3.4). Dip the control strips int
45、o the nutrient solution specified in Table 3 and hang them to dry in a sterile atmosphere. Freshly prepare the nutrient solution immediately before impregnating the control strips. Use the control strips on the day of their preparation.Using hydrochloric acid (HCl) or sodium hydroxide (NaOH) solutio
46、n, adjust the pH of the solution to 5,3.DIN ISO 9022-11:2016-02 8Table 3 Nutrient solution for soaking control stripsComponentMassgPotassium dihydrogen orthophosphate (KH2PO4) 0,1Ammonium nitrate (NH4NO3) 0,1Magnesium sulfate heptahydrate (MgSO4.7H2O) 0,025Yeast extract 0,05Glycerol C3H5(OH)3 10,0Di
47、stilled water (H2O) 90,03.4 SpecimenUnless the testing of complete instruments or assemblies is required in the relevant specification, representative samples shall be used for testing. Sample sheets of the size and dimensions shown in Figure 1 and of at least 1 mm thickness should preferably be use
48、d as representative samples of non-metallic coatings or lubricants to be tested.NOTE Sample sheets of 140 mm 2 mm or 280 mm 2 mm in length can also be indicated in the appropriate specification.Dimensions in millimetresKey1 marking area (on the back)a210 2 (280 2)b95 5 (140 2)Figure 1 Sample sheetTh
49、e coating under test (e.g. varnish) shall be of the same structure as the coating intended for the instrument or for parts of the instrument.Prior to applying the coating, prepare the surface of the sample sheet in the same manner as required for the original instrument. The coating shall completely surround the sample sheet so as to cover particularly edges, corners, and the edges of holes. The coating shall not be degraded by identification marking; numbers, etc. shall be punched prior to applying the coating.D
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