1、BS EN STD.BS1 BS EN 12225-ENGL 2000 m Lb24bb 08775LL 351 m BRITISH STANDARD Geotextiles and geotextile-related products - Method for determining the microbiological resistance by a soil burial test “he European Standard EN 122252000 has the status of a British standard ICs 69.080.70 12225:2000 NO CO
2、PYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYBIGET LAW STD.BS1 BS EN 12225-ENGL 2000 H I1624669 0877532 298 H BS EN 12226:2000 Amd. No. Date This British Standard, having been prepared under the direction of the Sector Committee for Builduig and Civil Engineering, was published under the
3、authority of the Standards Committee and comes into effect on 15 November 2000 Comments 0 Es1 11-2000 ISBN O 580 36549 2 National foreword This British Standard is the official English language version of EN 122252000. It supersedes DD ENV 12225 1997 which is withrawn The UK participation in its pre
4、paration was entrusted to Technical Committee W553, Geomembranes and geotextiles, which has the responsibility to: - aid enquirem to understand the te - present to the responsible European committee any enquiries on the - monitor related intemational and European developments and promulgate interpre
5、tation, or proposals for change, and keep the UK interests informed; themintheuK. A list of organizations represented on this committee can be obtained on request to its secretary. Cross-references The British Standards which implement international or European publications referred to in this docum
6、ent may be found in the BSI Standards Catalogue under the section entitied uIntemational Standards Correspondence Index”, or by using the “Find” facility of the BSI Standards Electronic Catalogue. A British Standard does not purport to include all the necessary provisions of a contract. Users of Bri
7、tish Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, the EN title page, pages 2 to 8, an inside back cover and a back
8、cover. The BSI copyright notice dispiayed in this document indicates when the document was last issued. STD.BS1 BS EN 12225-ENGL 2000 H 2624669 0877523 i124 EUROPEAN STANDARD EN 12225 NORME EUROPENNE EUROPASCHE NORM September 2000 ICs 07.1 00.99; 59.080.70 Supersedes ENV 122251996 English version Ge
9、otextiles and geotextile-related products - Method for determining the microbiological resistance by a soil burial test Gotextiles et produits apparent m, is the mass of solids. 4 Principle The test consists of exposing test specimens to microbially active soil under specified conditions. At the end
10、 of the exposure, the test specimens are evaluated visually, both before and after cleaning, and tested by measuring physical properties. These test results are compared with those obtained on unexposed specimens in accordance with EN 12226:2000. 5 Reagents and apparatus 5.1 Test soil The test soil
11、shall contain a variety of micro-organisms. To favour the optimum activity of the entire microbial population, the moisture content of the test soil shall be 60 % of SMC when determined according to method A.2 of prEN ISO/DIS 11721-?:I995 Natural soil collected in the field shall be sieved and all p
12、articles greater than 4 mm diameter removed. The water content is determined by drying 100 g of the soil in a thin layer at 103 “C to 105 “C until the mass remains constant to within 1 % (normally 24 h). If the water content of the test soil is too high, dry it in thin layers in the laboratory atmos
13、phere. Do not heat since this might affect the microflora. If necessary use a solution of I g ammonium nitrate and 0,2 g dipotassium-hydrogen phosphate per 1 I of water to increase the water content. To establish a constant microbial activity of the test soil, the soil shall be acclimatized prior to
14、 the soil burial test. The method consists of a preliminary incubation for at least one month at (97 I 2) % relative humidity and about 28 “C for a freshly obtained soil. If the same soil material is used more than once, then for each new test set up half of the old material shall be replaced by fre
15、sh soil material in the manner described above. Q BSI 11 -2000 STD-BSI BS EN 32225-ENGL 2000 9 1ib24bb9 0877537 87T Page 5 EN 12225:2000 NOTE: Good experience has been gained with soil type John Innes No. 2, or with Einheitserde ED 73, a soil which contains 66 % of ashable substances and 0,24 Oh of
16、nitrogen, the pH-value of the 10 Oh suspension is 5,2 and the SMC = 185 %. 5.2 Cotton fabric Strips, 100 mm long and 25 mm wide, of a bleached and untreated woven cotton fabric, mass per unit area about 250 g/d, shall be used as a reference to test the biological activity of the soil. NOTE: The foll
17、owing cotton fabrics have proved themselves as suitable: - Einfaserprfgewebe Baumwolle S/465 Nr. 600 465 O01 O01 5 (available from Loeffler Filtertechnik GmbH, Germany); - Control fabric, defined as follows: cotton American type, good middling; warp: 18,5 tex Z 886 x 2s 748; weft: 30 tex 2 630 x 2s
18、748; plain weave 34 endslcm by 17 pickskm, with brown and green threads at 0,5 cm and 1 ,O cm intervals so that strips with identical numbers of threads can be prepared; mass per unit area 230 g/m2; finish: scoured only (available from British Textile Technology Group, Manchester, UK). 5.3 Microbici
19、des An ethanol - water mixture 70:30 shall be used as a cleaning and disinfectant fluid after the soil burial test. 5.4 Containers The size and shape of the containers shall be such that the test specimens can be buried in a 100 mm to 150 mm depth of test soil. The dimensions of the containers shoul
20、d facilitate easy handling and placement in the test environment. If the containers are covered, it is necessary to provide the covers with ventilation openings for the access of oxygen to the test soil. 6 Specimens Prepare 1 O specimens according to EN 12226:2000 for each set of conditions. Store t
21、he control specimens at (22 f 2) “C and (65 k 5) % relative humidity. 7 Test climate A dark environment with admission of fresh air and air ventilation controlled at (95 f 5) % relative humidity and (26 f 1) “C shall be used to maintain the moisture content of the test soil at 60 % of SMC. Ensure th
22、at neither drying nor addition of water to the test soil during a test time of several weeks influences the performance of the microbes, and that ventilation of the test soil is not prevented. O BSI 11-2000 sTD.BSI BS EN 22225-ENGL 2000 2624669 08775LB 706 Page 6 EN 12225:2000 a Procedure 8.1 Biolog
23、ical activity of the soil Bury strips of the cotton fabric described in 5.2 and keep them in the test soil for seven days. After this exposure the tensile strength of the cotton strips shall be 5 25 % of the original tensile strength. If this criterion is not met, the soil shall be replaced by a bio
24、logically more active soil. The biological activity of the test soil shall be checked during the soil burial test according to 8.2 by adding a cotton strip to the test. On completion of the exposure, this strip shall have been destroyed. NOTE: The biological activity of the soil can be increased by
25、adding well rotted garden compost to the test soil. 8.2 Testing of specimens 8.2.1 Active test For the active test, set up at least two containers for each incubation time. The duration of the test shall be 16 weeks. Place a minimum of two test specimens and one cotton strip in each of the soil cont
26、ainers. Bury the specimens approximately 1 O0 mm deep and ensure good contact between them and the test soil. The container shall permit free oxygen exchange, and shall therefore not be closed. 8.2.2 Incubation Place the test containers prepared according to 8.2.1 in an incubator for 16 weeks at the
27、 climatic conditions specified under clause 7. NOTE: For index tests, the test duration is 16 weeks. During the development of new geotextiles and geotextile-related products a prolonged test of 32 weeks may be used. Control the moisture content to 60 % SMC by verifying the mass of the soil in the v
28、essels every four weeks. If necessary, ammonium nitrate-dipotassium hydrogen phosphate solution shall be added. 8.2.3 Cleaning of test specimens Recover the test specimens from the soil, strip off the soil, and submerge all specimens, including the control specimens, in an ethanol-water solution (70
29、:30) for 300 s. Then clean the specimens under running water, wipe with absorbent paper and dry for at least 72 h at (20 f 2) OC and (65 f 5) % RH. 8.2.4 Evaluation tests Determine the tensile strength of test and control specimens in accordance with EN 12226:2000. Q BSI 11 -2000 STD.BSI BS EN 12225
30、-ENGL 2000 m Lb24669 0877539 642 m Page 7 EN 122252000 9 Test report The test report shall include the following particulars: reference to this European Standard; identification of the sample; number of tested specimens; test set up and execution: type of soil, type of containers, type of cotton fab
31、ric; preparation of test soil, percentage of fresh test soil; test conditions: water content and biological activity of test soil, temperature and relative humidity; duration of test: date of start and end of soil burial; results of visual examination and microscopic inspection, including magnificat
32、ion factor; any other significant observations; changes in properties of the exposed samples as defined in EN 12226:2000; deviations from this European Standard. Q BSI 11-2000 STD-BSI BS EN 12225-ENGL 2000 = Lb24bb9 0877520 364 Page 8 EN 122252000 Annex A (informative) Bibliography CR IS0 13434, Gui
33、delines on durability of geotextiles and geotextile-related products. O BSI 11-2000 BS EN 12225:2000 BSI 389 Chiswick High Road London w4 4AL BSI - British Standards Institution BSI is the independent naonal body responsible for preparing British Standards. It presents the UK view on standards in Eu
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