EN 46-2-2016 en Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 2 Ovicidal effect (laboratory me.pdf

1、BS EN 46-2:2016W o o d p r e s e r v a t i v e s Determination of thepreventive action againstr e c e n t l y h a t c h e d l a r v a e o fHylotrupes bajulus (Linnaeus)Part 2: Ovicidal effect (laboratory method)BSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06BS EN 46

2、-2:2016 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of EN 46-2:2016.It supersedes BS EN 46-2:2009 which is withdrawn .The UK participation in its preparation was entrusted to TechnicalCommittee B/515, Wood preservation.A list of organizations represented on this c

3、ommittee can beobtained on request to its secretary.This publication does not purport to include all the necessaryprovisions of a contract. Users are responsible for its correctapplication. The British Standards Institution 2016 .Published by BSI Standards Limited 2016ISBN 978 0 580 90449 3ICS 71.10

4、0.50Compliance with a British Standard cannot confer immunity fromlegal obligations.This British Standard was published under the authority of theStandards Policy and Strategy Committee on 30 June 2016.Amendments/corrigenda issued since publicationDate T e x t a f f e c t e dBS EN 46-2:2016EUROPEAN

5、STANDARD NORME EUROPENNE EUROPISCHE NORM EN 46-2 June 2016 ICS 71.100.50 Supersedes EN 46-2:2009English Version Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 2: Ovicidal effect (laboratory method) Produits de prser

6、vation du bois - Dtermination de laction prventive contre les larves rcemment closes dHylotrupes bajulus (Linnaeus) - Partie 2: Effet ovicide (Mthode de laboratoire) Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenber frisch geschlpften Larven von Hylotrupes bajulus (Linnaeus) - Teil 2:

7、Ovizide Wirkung (Laboratoriumsverfahren) This European Standard was approved by CEN on 5 January 2017. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

8、Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by tra

9、nslation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finl

10、and, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey andUnited Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATIO

11、N COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2016 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 46-2:2016 EBS EN 46-2:2016EN 46-2:2016 (E) 2 Cont

12、ents Page European foreword 4 Introduction 5 1 Scope 6 2 Normative references 6 3 Terms and definitions . 6 4 Principle . 7 5 Test materials 7 5.1 Biological material . 7 5.2 Products and reagents 7 5.3 Apparatus 7 6 Sampling . 8 7 Test specimens 8 7.1 Species of wood 8 7.2 Wood quality 8 7.3 Provis

13、ion of test specimens 9 7.4 Dimensions of test specimens 9 7.5 Number of test specimens 9 7.5.1 Test specimens for egg-laying 9 7.5.2 Test specimens for checking the tunnelling ability and the mortality of the larvae . 10 8 Procedure 10 8.1 Preparation of the test specimens 10 8.1.1 Conditioning of

14、the test specimens prior to sealing . 10 8.1.2 Sealing of block faces . 10 8.1.3 Treatment of the test specimens . 10 8.1.4 Drying and conditioning of the test specimens after treatment 12 8.2 Exposure of the test specimens to the insects (ovicidal action) 12 8.3 Validity of the test . 13 9 Expressi

15、on of results . 13 9.1 Ovicidal test 13 9.2 Tunnelling control 13 9.3 Total mortality . 14 10 Test report . 14 Annex A (informative) Example of a test report . 16 Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus) 18 B.1 General 18 B.2 Obtaining parent beetles 18 B.3 Mating

16、. 18 BS EN 46-2:2016EN 46-2:2016 (E) 3 B.4 Egg-Iaying 18 B.5 Hatching of eggs 19 B.6 Larval development . 19 B.7 Enemies and parasites 20 Annex C (informative) Differentiation of heartwood and sapwood in Pinus species 21 C.1 Principle . 21 C.2 Reagents . 21 C.3 Apparatus 21 C.4 Procedure 21 Annex D

17、(informative) Environmental, health and safety precautions within chemical/biological laboratory . 22 Bibliography . 23 BS EN 46-2:2016EN 46-2:2016 (E) 4 European foreword This document (EN 46-2:2016) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and wood-based products”, th

18、e secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2016, and conflicting national standards shall be withdrawn at the latest by December 2016. Attent

19、ion is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN 46-2:2009. Significant technical differences between this docume

20、nt and EN 46-2:2009 are as follows: a) introduction of new harmonised specifications for wood quality; b) option to omit control test specimens treated with the solvent or diluents only when the solvent or diluents is water of drinking quality. The standard EN 46 is composed of two parts: EN 46-1, W

21、ood preservatives Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 1: Application by surface treatment (laboratory method) EN 46-2, Wood preservatives Determination of the preventive action against recently hatched larvae of Hylotrupes baju

22、lus (Linnaeus) Part 2: Ovicidal effect (laboratory method) EN 46 consists of two parts to enable preventive action of wood preservatives, against recently hatched larvae of Hylotrupes bajulus, which are intended to be applied by surface treatment; Part 1 is required to determine the larvicidal effec

23、t of preservatives and Part 2 is required to determine the ovicidal action of the preservatives after egg-laying of young females. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria

24、, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden,

25、Switzerland, Turkey and the United Kingdom. BS EN 46-2:2016EN 46-2:2016 (E) 5 Introduction This test method describes a laboratory method of test which gives a basis for the assessment of the preventive action of a wood preservative, when applied as a surface treatment for timber, against eggs of Hy

26、lotrupes bajulus. In combination with EN 46-1 it provides a means of checking whether larvae may hatch from eggs laid on the treated wood surface and whether they are capable of boring through the treated surface and of surviving in the untreated part of the wood. This standard provides information

27、for the sealing of all but one lateral face when specimens are to be treated by dipping. When products which are very active at very low concentration are used, it is very important to take suitable precautions to isolate and separate, as far as possible, operations involving chemical products, othe

28、r products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel (see also Annex D for environmental, health and safety precautions). BS EN 4

29、6-2:2016EN 46-2:2016 (E) 6 1 Scope This European Standard specifies a method for the determination of the preventive action of a wood preservative against eggs of Hylotrupes bajulus (Linnaeus) when the preservative is applied as a surface treatment to wood. This method is applicable to: water-insolu

30、ble chemicals which are being studied as active insecticides; organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates; organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; or water-soluble materials, f

31、or example salts. The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures. 2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated ref

32、erences, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN 46-1, Wood preservatives Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 1: Application b

33、y surface treatment (laboratory method) EN 73, Wood preservatives Accelerated ageing of treated wood prior to biological testing Evaporative ageing procedure EN 84, Wood preservatives Accelerated ageing of treated wood prior to biological testing Leaching procedure EN ISO 3696, Water for analytical

34、laboratory use Specification and test methods (ISO 3696) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample having its physical and/or chemical characteristics identical to the volumetric average characteristics of t

35、he total volume being sampled SOURCE: EN 1001-2:2005, 4.71 BS EN 46-2:2016EN 46-2:2016 (E) 7 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to be tested) Note 1 to entry: Adapted from EN 10012:2005, 4.83. 4 Principle In this laboratory method treated wo

36、od panels are offered to freshly mated Hylotrupes bajulus females. The hatching ability of the larvae on the treated timber is examined. When the ovicidal action is insufficient, the mortality of the hatched larvae on and/or in wood treated with the same formulation is also established according to

37、EN 46-1. 5 Test materials 5.1 Biological material 5.1.1 Hylotrupes bajulus (Linnaeus) females. 5.1.2 Source of females The insects shall preferably be obtained from cultures reared as e.g. described in Annex B. Use only sound and lively insects. 5.2 Products and reagents 5.2.1 Paraffin wax, for fixi

38、ng the glass plate in all cases and for sealing the end faces of test specimens to be treated with solutions in all cases in which water is the continuous phase. NOTE Paraffin wax with a setting point of 52 C to 54 C has been found to be suitable. 5.2.2 Gelatine, for sealing the end faces of test sp

39、ecimens to be treated with solutions in which an organic solvent is the continuous phase. 5.2.3 Water, complying with grade 3 of EN ISO 3696. 5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-trea

40、tment conditioning period that has a toxic effect on the insects. CAUTION Do not use benzene or other solvents which pose a health risk. 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, and controlled at (28 2) C and at relative humidity (70 5) %. 5.3.2 Conditioning chamber, well ventila

41、ted and controlled at (20 2) C and at relative humidity (65 5) %. The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.3) provided that this has the conditions specified for the conditioning chamber (see 5.3.2). BS EN 46-2:2016EN 46-2:2016 (E) 8 5.3.3 Laboratory

42、 work area, well ventilated, where treatment of the test specimens is carried out. CAUTION It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours. 5.3.4 Testing chamber, ventilated and air-conditioned

43、, controlled at (22 2) C and at relative humidity between (70 5) %. 5.3.5 Petri dishes of glass or polyvinylchloride (PVC), diameter ca. 9 cm for mating the insects and for egg-laying. 5.3.6 Weights, to provide ballast for the test specimens. The weights shall not react with any materials with which

44、 they come into contact during the test. 5.3.7 Safety equipment, protective clothing, appropriate for the test product and the test solvent, to ensure the safety of the operator. 5.3.8 Glass plates, (48 1) mm long and (25 1) mm wide, intended to provide a lateral slit on the test specimens. 5.3.9 Or

45、dinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g and equipment for applying a liquid product by brushing or by pipette. 5.3.10 Protective gloves 6 Sampling The sample of preservative shall be representative of the product to be tested. Samples shall be st

46、ored and handled in accordance with any written recommendations from the supplier. For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used. 7 Test specimens 7.1 Species of wood The reference species is Scots pine (Pinus sylvestris Linnaeus)1). Additional te

47、sts may be carried out using other species but, if so, this shall be stated in the test report. 7.2 Wood quality The wood shall be free from visible cracks, stain, decay, insect damage and other defects. The wood shall not have been water-stored, floated, chemically treated or steamed. The wood shal

48、l originate from trees preferably felled in winter. The trees shall be cut immediately after felling 1)In southern European countries the pine species most frequently infested by Hylotrupes bajulus may be used as an alternative, provided that the suitability of the species for use in the tests speci

49、fied in this standard has been demonstrated in all aspects (development of larvae, resistance of impregnation, etc.). BS EN 46-2:2016EN 46-2:2016 (E) 9 and the timber rapidly air dried or kiln dried at temperatures below 60 C. The wood shall not have been stored for more than five years. The wood shall be exclusively sapwood containing little resin and having between 2,5 annual rings per 10 mm and eight annual rings per 10 mm. The proportion of latewood in the annual rings shall not exceed 30 % of the whole