EN 49-1-2016 en Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 1 Application by sur.pdf

1、BS EN 49-1:2016Wood preservatives Determination of theprotective effectiveness againstAnobium punctatum (DeGeer) by egg-laying and larvalsurvivalPart 1: Application by surface treatment(Laboratory method)BSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06BS EN 49-1:2016

2、 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of EN 49-1:2016. Itsupersedes BS EN 49-1:2005 which is withdrawn.The UK participation in its preparation was entrusted to TechnicalCommittee B/515, Wood preservation.A list of organizations represented on this committee

3、 can beobtained on request to its secretary.This publication does not purport to include all the necessaryprovisions of a contract. Users are responsible for its correctapplication. The British Standards Institution 2016. Published by BSI StandardsLimited 2016ISBN 978 0 580 90447 9ICS 71.100.50Compl

4、iance with a British Standard cannot confer immunity fromlegal obligations.This British Standard was published under the authority of theStandards Policy and Strategy Committee on 30 June 2016.Amendments issued since publicationDate Text affectedBS EN 49-1:2016EUROPEAN STANDARD NORME EUROPENNE EUROP

5、ISCHE NORM EN 49-1 June 2016 ICS 71.100.50 Supersedes EN 49-1:2005English Version Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 1: Application by surface treatment (Laboratory method) Produits de prserv

6、ation du bois - Dtermination de lefficacit protectrice vis-vis dAnobium punctatum (De Geer) par lobservation de la ponte et du taux de survie des larves - Partie 1: Application par traitement de surface (Mthode de laboratoire) Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenber Anobium p

7、unctatum (De Geer) durch Beobachten der Eiablage und des berlebens von Larven - Teil 1: Oberflchenverfahren (Laboratoriumsverfahren) This European Standard was approved by CEN on 5 January 2017. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions

8、for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in th

9、ree official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards b

10、odies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia,

11、 Spain, Sweden, Switzerland, Turkey andUnited Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2016 CEN All rights of exploitation in any form and by any means reserved world

12、wide for CEN national Members. Ref. No. EN 49-1:2016 EBS EN 49-1:2016EN 49-1:2016 (E) 2 Contents Page European foreword 4 Introduction . 5 1 Scope . 6 2 Normative references . 6 3 Terms and definitions 6 4 Principle 6 5 Test materials 7 5.1 Biological material . 7 5.2 Products and reagents 7 5.3 App

13、aratus 8 6 Sampling 8 7 Test specimens 8 7.1 Species of wood . 8 7.2 Wood quality 9 7.3 Provision of test specimens 9 7.4 Dimensions of test specimens . 9 7.5 Number of test specimens . 9 8 Procedure. 10 8.1 Preparation of the test specimens 10 8.1.1 Conditioning of test specimens prior to sealing 1

14、0 8.1.2 Sealing . 10 8.1.3 Treatment of the test specimens . 10 8.1.4 Drying and conditioning of the test specimens after treatment 11 8.2 Exposure of the test specimens to the insects 12 8.3 Conditions and duration of the test 12 8.4 Examination of the test specimens . 12 9 Validity of test . 12 10

15、 Expression of results 13 10.1 Assessment of the protective effectiveness . 13 10.2 Toxic values . 13 11 Test report . 13 Annex A (informative) Example of a test report . 15 Annex B (informative) Identification of sex of test insects Anobium punctatum 17 Annex C (informative) Culturing technique for

16、 Anobium punctatum . 18 C.1 Culture wood . 18 C.1.1 Wood species 18 C.1.2 Collection of culture wood . 18 C.1.3 Cutting of culture wood . 18 BS EN 49-1:2016EN 49-1:2016 (E) 3 C.1.4 Drying of culture wood 18 C.2 Source of beetles 18 C.2.1 Collection of beetles 18 C.2.2 Quarantine of beetles . 18 C.3

17、Infestation of culture wood 18 C.3.1 Culture vessels 18 C.3.2 Preparation of wood . 18 C.3.3 Introduction of beetles 19 C.4 Culturing conditions . 19 C.4.1 Normal environment 19 C.4.2 Natural pupation induction . 19 C.4.3 Artificial pupation induction . 19 C.5 Collection of beetles 19 C.6 General cu

18、lture hygiene 19 Annex D (informative) Environmental, health and safety precautions within chemical/biological laboratory 21 Bibliography 22 BS EN 49-1:2016EN 49-1:2016 (E) 4 European foreword This document (EN 49-1:2016) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and woo

19、d-based products”, the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2016, and conflicting national standards shall be withdrawn at the latest by

20、December 2016. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN 49-1:2005. Significant technical difference

21、s between this document and EN 49-1:2005 are as follows: a) generalization of material for preparing the egg-laying zones; b) introduction of new harmonized specifications for wood quality. This document consists of two parts, Part 1 is required to enable effectiveness assessments of wood preservati

22、ves that are intended to be applied by surface treatment and Part 2 those that are intended to be applied by impregnation. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgiu

23、m, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerl

24、and, Turkey and the United Kingdom. BS EN 49-1:2016EN 49-1:2016 (E) 5 Introduction This document describes a laboratory method of testing which gives a basis for assessment of the effectiveness of a wood preservative, when applied as a surface treatment, against Anobium punctatum. It allows the dete

25、rmination of the concentration at which the product prevents the development of infestation from egg laying. It can also be used with formulations ready for use. The method simulates conditions that can occur in practice on timber which has been treated some time previously with wood preservative ap

26、plied by dip, brush or spray and on which eggs of Anobium punctatum are laid. This laboratory method provides one criterion by which the value of a product can be assessed. In making this assessment the methods by which the preservative may be applied should be taken into account. It is further reco

27、mmended that results from this test should be supplemented by those from other appropriate tests, and above all by comparison with practical experience. When products which are very active at low concentrations are used it is very important to take suitable precautions to isolate and separate, as fa

28、r as possible, operations involving chemical products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel (see also Annex D

29、 for environmental, health and safety precautions). BS EN 49-1:2016EN 49-1:2016 (E) 6 1 Scope This European Standard specifies a method for the determination of the protective effectiveness or the toxic values of a wood preservative against infestation by Anobium punctatum (De Geer) when the product

30、 is applied as a surface treatment to wood. This method is applicable to: water-insoluble chemicals that are being studied as active insecticides; organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates; organic water-dispersible formulations as supplied or as

31、prepared in the laboratory by dilution of concentrates; water-soluble materials, for example salts. NOTE This method may be used in conjunction with an ageing procedure, for example EN 73. 2 Normative references The following documents, in whole or in part, are normatively referenced in this documen

32、t and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN ISO 835, Laboratory glassware Graduated pipettes (ISO 835) EN ISO 3696, Water for analytical

33、 laboratory use Specification and test methods (ISO 3696) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample having its physical or chemical characteristics identical to the volumetric average characteristics of the

34、total volume being sampled SOURCE: EN 1001-2:2005, 4.71 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to be tested) Note 1 to entry: Adapted from EN 10012:2005, 4.83. 4 Principle Depending on the test being carried out either: on a set of test specimen

35、s of a susceptible wood species that is surface treated with a solution of the preservative, or BS EN 49-1:2016EN 49-1:2016 (E) 7 if toxic values are to be determined, on several sets of test specimens of a susceptible wood species that are surface treated with a series of solutions in which the con

36、centration of preservative is ranged in a given progression. The treated test specimens are exposed to gravid females of Anobium punctatum. The number of eggs laid, the number of eggs hatched and the numbers of surviving larvae are observed and compared with those in untreated control test specimens

37、. If the preservative has been prepared in the laboratory by dilution of a concentrate or by dissolution of a solid, the resulting attack is also compared to that in solvent or diluent treated control test specimens. 5 Test materials 5.1 Biological material Anobium punctatum (De Geer) Adult males an

38、d females in good condition. Adults to be used in the test shall be collected at daily intervals from naturally infested wood or laboratory culture (see Annex C). Use recently emerged adults which have been recently collected; kept overnight in quarantine (see C.6); and then checked to ensure that t

39、hey are undamaged, active and free from any infestation by mites. Determine the sex (see Annex B) of the collected and checked adults and place the males and females in separate containers. NOTE The proportion of males and females varies during the emergence period. 5.2 Products and reagents 5.2.1 P

40、araffin wax, for sealing the relevant faces of test specimens to be treated with solutions in which water is the continuous phase. NOTE Paraffin wax with a setting point of 52 C to 54 C has been found to be suitable. 5.2.2 Gelatin, for sealing the relevant faces of test specimens to be treated with

41、solutions in which an organic solvent is the continuous phase. 5.2.3 Paste, for securing filter paper. The paste shall be starch-free, non-toxic to Anobium punctatum and insoluble in the product under test. NOTE Sodium carboxy methyl cellulose, food grade, has been found to be suitable. 5.2.4 Water,

42、 complying with grade 3 of EN ISO 3696. 5.2.5 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects. CAUTION Do not use benzene or other

43、solvents which pose a health risk. 5.2.6 Filter paper, ordinary quality, medium-fast grade. 5.2.7 Fine cloth of a suitable material with a mesh aperture of 0,3 mm to 0,6 mm for the preparation of the egg-laying zones. NOTE Cotton, linen and polyamide-gauze have been proven as suitable. BS EN 49-1:20

44、16EN 49-1:2016 (E) 8 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, controlled at (21 2) C, and at relative humidity (80 5) %. 5.3.2 Conditioning chamber, well ventilated, controlled at (20 2) C and at relative humidity (65 5) %. The conditioning of test specimens may be carried out in

45、 the laboratory work area (see 5.3.4) provided that this has the conditions specified for the conditioning chamber (see 5.3.2). 5.3.3 Treatment vessel, of a material that does not react with the preservative under test, for example of glass for organic products and of polyethylene for salts containi

46、ng fluorine. 5.3.4 Laboratory work area, well ventilated, where treatment of the test specimens is carried out. CAUTION It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours. 5.3.5 Testing chamber, w

47、ith conditions identical to those of the culturing chamber (see 5.3.1). 5.3.6 Pipette, of type specified in EN ISO 835, Class B: graduated pipette with no waiting time. Capacity 1 ml with an accuracy of 0,01 ml. 5.3.7 Safety equipment and protective clothing, appropriate for the test product and the

48、 test solvent, to ensure the safety of the operator. 5.3.8 Test containers, suitable for holding the test specimens and of material resistant to the solvents used, and fitted with perforated covers to provide a good exchange of air. NOTE Jars of approximately 60 mm diameter and 100 mm height have be

49、en found to be suitable. 5.3.9 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g. 5.3.10 X-ray apparatus, (optional) with tungsten target and beryllium window, with voltage and current continuously variable in the ranges: voltage: 10 kV to 50 kV; current: 0 mA to 15 mA. 5.3.11 Protective gloves 6 Sampling The sample of preservative shall be representative of the product to be tested. Samples shall be stored and handled in accordance with any written recommendations from the supplier