EN ISO 7218-2007 en Microbiology of food and animal feeding stuffs - General requirements and guidance for microbiological examinations (Incorporates Amendment A1 2013)《食物和动物饲料的微生物.pdf

1、BRITISH STANDARDBS EN ISO 7218:2007IncorporatingcorrigendumMicrobiology of food and animal feeding stuffs General requirements and guidance for microbiological examinationsICS 07.100.30 BS EN ISO +A1:2013Incorporating BS EN ISO corrigenda March 2009 and September 2015EUROPEAN STANDARDNORMEEUROPENNEE

2、UROPISCHENORMEN ISO 7218August 2007ICS 07.100.30English VersionMicrobiology of food and animal feeding stuffs - Generalrequirements and guidance for microbiological examinations(ISO 7218:2007)Microbiologie des aliments - Exigences gnrales etrecommendations (ISO 7218:2007)Mikrobiologie von Lebensmitt

3、eln und Futtermitteln -Allgemeine Anforderungen und Anleitung frmikrobiologische Untersuchungen (ISO 7218:2007)This European Standard was approved by CEN on 19 April 2007.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European

4、Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the CEN Management Centre or to any CEN member.This European Standard exists in three official versions (English, Fre

5、nch, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as theofficial versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, C

6、zech Republic, Denmark, Estonia, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMIT EUROP

7、EN DE NORMALISATIONEUROPISCHES KOMITEE FR NORMUNGManagement Centre: rue de Stassart, 36 B-1050 Brussels 2007 CEN All rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN ISO 7218:2007: EEN ISO 7218:2007+A1August 2013BS EN ISO 7218:2007+A1:2013ISB

8、N 978 0 580 91714 1Amendments/corrigenda issued since publicationDate Comments31 May 2009 Correction to the Supersession details in the National Foreword31 August 2013 Implementation of ISO amendment 1:2013 with CEN endorsement A1:201330 September 2015 Implementation of ISO corrected text 15 April 2

9、014: Tables C.5, C.6 and C.7 updatedThis British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 March 2008 The British Standards Institution 2015. Published by BSI Standards Limited 2015National forewordThis British Standard is the UK implementation o

10、f EN ISO 7218:2007+A1:2013. It is identical to EN ISO 7218:2007, incorporating amendment 1:2013. It supersedes BS EN ISO 7218:2007, which is withdrawn.The start and finish of text introduced or altered by amendment is indicated in the text by tags. Tags indicating changes to ISO text carry the numbe

11、r of the ISO amendment. For example, text altered by ISO amendment 1 is indicated by .The UK participation in its preparation was entrusted to Technical Committee AW/9, Microbiology.A list of organizations represented on this committee can be obtained on request to its secretary.BS EN ISO 7218 descr

12、ibes the requirements and recommendations for good laboratory practice in a microbiology laboratory that help to ensure the general quality of food testing. Laboratories would be expected to take account of the principles described in BS EN ISO 7218 in order to demonstrate competence in accordance w

13、ith ISO/IEC 17025.The publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application.Compliance with a British Standard cannot confer immunity from legal obligations.EUROPEAN STANDARDNORME EUROPENNEEUROPISCHE NORMEN ISO 7218Augus

14、t 2007ICS 07.100.30English VersionMicrobiology of food and animal feeding stuffs - Generalrequirements and guidance for microbiological examinations(ISO 7218:2007)Microbiologie des aliments - Exigences gnrales etrecommendations (ISO 7218:2007)Mikrobiologie von Lebensmitteln und Futtermitteln -Allgem

15、eine Anforderungen und Anleitung frmikrobiologische Untersuchungen (ISO 7218:2007)This European Standard was approved by CEN on 19 April 2007.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a nati

16、onal standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the CEN Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in an

17、y other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as theofficial versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Eston

18、ia, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMIT EUROPEN DE NORMALISATIONEUROPISCHE

19、S KOMITEE FR NORMUNGManagement Centre: rue de Stassart, 36 B-1050 Brussels 2007 CEN All rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN ISO 7218:2007: EEN ISO 7218:2007+A1August 2013Foreword This document (EN ISO 7218:2007) has been prepared

20、 by Technical Committee ISO/TC 34 “Agricultural foodproducts“ in collaboration with Technical Committee CEN/TC275 “Food analysis - Horizontal methods“ the secretariat of which is held by DIN. This European Standard shall be given the status ofa national standard, either by publication of an identica

21、l text or by endorsement, at the latest by February2008, and conflicting national standards shall be withdrawnat the latest by February 2008. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the followingcountries are bound to implement this European Standar

22、d: Austria, Belgium, Bulgaria, Cyprus, CzechRepublic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the UnitedKingdom. Endor

23、sement notice The text of ISO 7218:2007 has been approved by CEN as a EN ISO 7218:2007 without any modification. BS EN ISO 7218:2007iiBS EN ISO 7218:2007+A1:2013EN ISO 7218:2007+A1:2013Foreword to amendment A1EN ISO 7218:2007/A1:2013 (E)3ForewordThis document (EN ISO 7218:2007/A1:2013) has been prep

24、ared by Technical Committee ISO/TC 34 “Food products“ in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of which is held by DIN.This European Standard shall be given the status of a national standard, either by publication of an identical text

25、or by endorsement, at the latest by February 2014, and conflicting national standards shall be withdrawn at the latest by February 2014Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsibl

26、e for identifying any or all such patent rights.According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Fo

27、rmer Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.Endorsement noticeThe text of ISO 7218:2

28、007/Amd 1:2013 has been approved by CEN as EN ISO 7218:2007/A1:2013 without any modification.iiiContents PageForeword. vIntroduction. vi1Scope . 12Normative references. 13Premises 23.1 General. 23.2 Safety considerations. 23.3 Laboratory design. 23.4 Laboratory areas. 33.5 Layout and fittings of the

29、 premises 43.6 Cleaning and disinfection 54Staff 54.1 General. 54.2 Competence 64.3 Verification of on-going staff competence. 64.4 Hygiene 65Apparatus and equipment. 65.1 General. 65.2 Protective cabinets. 75.3 Balances and gravimetric diluters 85.4 Homogenizers, blenders and mixers 95.5 pH meter 1

30、05.6 Autoclave.115.7 Media preparator. 125.8 Incubator 125.9 Refrigerator, cold-storage room 135.10 Freezer and deep freezer . 145.11 Thermostatically controlled bath155.12 Steamers, including boiling-water baths 165.13 Sterilizing oven . 175.14 Microwave oven 175.15 Glass washer. 185.16 Optical mic

31、roscope. 195.17 Gas burner or wire incinerator 195.18 Dispenser for culture media and reagents.205.19Vortex mixer 205.20 Colony-counting device. 215.21 Equipment for culture in a modified atmosphere 225.22 Centrifuge 225.23 Hotplate and heating mantle 235.24 Spiral plater.235.25 Stills, deionizers a

32、nd reverse-osmosis units.245.26Timers and timing devices. 245.27 Pipettes and pipettors 255.28 Thermometers and temperature-monitoring devices, including automatic recorders. 265.29 Immunomagnetic separator. 275.30 Filtration system. 275.31 Other equipment and software28BS EN ISO 7218:2007iiiContent

33、s PageForeword. vIntroduction. vi1Scope . 12Normative references.3Premises 23.1 General. 23.2 Safety considerations. 23.3 Laboratory design. 23.4 Laboratory areas. 33.5 Layout and fittings of the premises 43.6 Cleaning and disinfection 54Staff4.1 General. 54.2 Competence 64.3 Verification of on-goin

34、g staff competence. 64.4 Hygiene 65Apparatus and equipment.5.1 General. 65.2 Protective cabinets. 75.3 Balances and gravimetric diluters 85.4 Homogenizers, blenders and mixers 95.5 pH meter 105.6 Autoclave.115.7 Media preparator. 125.8 Incubator 125.9 Refrigerator, cold-storage room 135.10 Freezer a

35、nd deep freezer . 145.11 Thermostatically controlled bath155.12 Steamers, including boiling-water baths 165.13 Sterilizing oven . 175.14 Microwave oven 175.15 Glass washer. 185.16 Optical microscope. 195.17 Gas burner or wire incinerator 195.18 Dispenser for culture media and reagents.205.19Vortex m

36、ixer 205.20 Colony-counting device. 215.21 Equipment for culture in a modified atmosphere 225.22 Centrifuge 225.23 Hotplate and heating mantle 235.24 Spiral plater.235.25 Stills, deionizers and reverse-osmosis units.245.26Timers and timing devices. 245.27 Pipettes and pipettors 255.28 Thermometers a

37、nd temperature-monitoring devices, including automatic recorders. 265.29 Immunomagnetic separator. 275.30 Filtration system. 275.31 Other equipment and software28BS EN ISO 7218:2007BS EN ISO 7218:2007+A1:2013ISO 7218:2007+A1:2013iii14182113Foreword This document (EN ISO 7218:2007) has been prepared

38、by Technical Committee ISO/TC 34 “Agricultural foodproducts“ in collaboration with Technical Committee CEN/TC275 “Food analysis - Horizontal methods“ the secretariat of which is held by DIN. This European Standard shall be given the status ofa national standard, either by publication of an identical

39、 text or by endorsement, at the latest by February2008, and conflicting national standards shall be withdrawnat the latest by February 2008. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the followingcountries are bound to implement this European Standard

40、: Austria, Belgium, Bulgaria, Cyprus, CzechRepublic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the UnitedKingdom. Endors

41、ement notice The text of ISO 7218:2007 has been approved by CEN as a EN ISO 7218:2007 without any modification. BS EN ISO 7218:2007iiBS EN ISO 7218:2007+A1:2013EN ISO 7218:2007+A1:2013Foreword to amendment A1EN ISO 7218:2007/A1:2013 (E)3ForewordThis document (EN ISO 7218:2007/A1:2013) has been prepa

42、red by Technical Committee ISO/TC 34 “Food products“ in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of which is held by DIN.This European Standard shall be given the status of a national standard, either by publication of an identical text o

43、r by endorsement, at the latest by February 2014, and conflicting national standards shall be withdrawn at the latest by February 2014Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible

44、 for identifying any or all such patent rights.According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, For

45、mer Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.Endorsement noticeThe text of ISO 7218:20

46、07/Amd 1:2013 has been approved by CEN as EN ISO 7218:2007/A1:2013 without any modification.iiiContents PageForeword. vIntroduction. vi1Scope . 12Normative references. 13Premises 23.1 General. 23.2 Safety considerations. 23.3 Laboratory design. 23.4 Laboratory areas. 33.5 Layout and fittings of the

47、premises 43.6 Cleaning and disinfection 54Staff 54.1 General. 54.2 Competence 64.3 Verification of on-going staff competence. 64.4 Hygiene 65Apparatus and equipment. 65.1 General. 65.2 Protective cabinets. 75.3 Balances and gravimetric diluters 85.4 Homogenizers, blenders and mixers 95.5 pH meter 10

48、5.6 Autoclave.115.7 Media preparator. 125.8 Incubator 125.9 Refrigerator, cold-storage room 135.10 Freezer and deep freezer . 145.11 Thermostatically controlled bath155.12 Steamers, including boiling-water baths 165.13 Sterilizing oven . 175.14 Microwave oven 175.15 Glass washer. 185.16 Optical micr

49、oscope. 195.17 Gas burner or wire incinerator 195.18 Dispenser for culture media and reagents.205.19Vortex mixer 205.20 Colony-counting device. 215.21 Equipment for culture in a modified atmosphere 225.22 Centrifuge 225.23 Hotplate and heating mantle 235.24 Spiral plater.235.25 Stills, deionizers and reverse-osmosis units.245.26Timers and timing devices. 245.27 Pipettes and pipettors 255.28 Thermometers and temperature-monitori