EN ISO 734-2-2010 8125 Oilseed meals - Determination of oil content - Part 2 Rapid extraction method《油料种子作物 含油量测定 第2部分 快速萃取法》.pdf

1、BS EN ISO734-2:2010ICS 67.200.20NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBRITISH STANDARDOilseed meals Determination of oilcontentPart 2: Rapid extraction method(ISO 734-2:2008, corrected version2009-06-01)Copyright European Committee for Standardization Provided by IHS

2、under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-This British Standardwas published under theauthority of the StandardsPolicy and StrategyCommittee on 31 May2010. BSI 2010ISBN 978 0 580 69812 5Amendments/corrigenda issued since publicationDate

3、CommentsBS EN ISO 734-2:2010National forewordThis British Standard is the UK implementation of EN ISO 734-2:2010.It is identical to ISO 734-2:2008. It supersedes BS EN ISO 734-2:2008which is withdrawn.The UK participation in its preparation was entrusted to TechnicalCommittee AW/307, Oil seeds, anim

4、al and vegetable fats and oils andtheir by products.A list of organizations represented on this committee can be obtained onrequest to its secretary.This publication does not purport to include all the necessary provisionsof a contract. Users are responsible for its correct application.Compliance wi

5、th a British Standard cannot confer immunityfrom legal obligations.Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 734-2

6、April 2010 ICS 67.200.20 Supersedes EN ISO 734-2:2008English Version Oilseed meals - Determination of oil content - Part 2: Rapid extraction method (ISO 734-2:2008, corrected version 2009-06-01) Tourteaux de graines olagineuses - Dtermination de la teneur en huile - Partie 2: Mthode rapide par extra

7、ction (ISO 734-2:2008, version corrige 2009-06-01) lsamenschrote - Bestimmung des lgehaltes - Teil 2: Schnellextraktionsverfahren (ISO 734-2:2008, korrigierte Fassung 2009-06-01) This European Standard was approved by CEN on 13 March 2010. CEN members are bound to comply with the CEN/CENELEC Interna

8、l Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member

9、. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions. CEN members

10、 are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sw

11、eden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2010 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members

12、. Ref. No. EN ISO 734-2:2010: ECopyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN ISO 734-2:2010EN ISO 734-2:2010 (E) 3 Foreword The text of ISO 734-2:2008, corrected version

13、2009-06-01 has been prepared by Technical Committee ISO/TC 34 “Food products” of the International Organization for Standardization (ISO) and has been taken over as EN ISO 734-2:2010 by Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their by-products - Methods of sa

14、mpling and analysis” the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by October 2010, and conflicting national standards shall be withdrawn at the latest by

15、 October 2010. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 734-2:2008. According to the CEN/CENELE

16、C Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,

17、 Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. Endorsement notice The text of ISO 734-2:2008, corrected version 2009-06-01 has been approved by CEN as a EN ISO 734-2:2010 without any modification. Copyright E

18、uropean Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-ISO 734-2:2008(E) ISO 2008 All rights reserved iiiForeword ISO (the International Organization for Standardization) is a worldwide federation

19、 of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee.

20、International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance wit

21、h the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approv

22、al by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 734-2 was prepared by Technical Committe

23、e ISO/TC 34, Food products, Subcommittee SC 2, Oleaginous seeds and fruits and oilseed meals. This second edition cancels and replaces the first edition (ISO 734-2:1998), which has been technically revised. ISO 734 consists of the following parts, under the general title Oilseed meals Determination

24、of oil content: Part 1: Extraction method with hexane (or light petroleum) Part 2: Rapid extraction method This corrected version of ISO 734-2:2008 incorporates the following corrections: on p. 2, “and materials” has been deleted from the Clause 6 title; on p. 3, a revised Figure 2 now indicates tol

25、erances to the dimensions. BS EN ISO 734-2:2010Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN ISO 734-2:2010Copyright European Committee for Standardization Provided by IH

26、S under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-INTERNATIONAL STANDARD ISO 734-2:2008(E) ISO 2008 All rights reserved 1Oilseed meals Determination of oil content Part 2: Rapid extraction method 1 Scope This part of ISO 734 specifies an extra

27、ction method which may be used to assess the efficiency of a de-oiling process by comparing the oil content of the oilseed with the residual oil content of the corresponding extraction meals, pellets and expeller cakes. It is not applicable to disputed cases, for which ISO 734-1 is applicable. It is

28、 applicable to oilseed meals obtained from oilseeds by expelling or by extraction with a solvent, as well as to the pellets made from the residues. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition

29、 cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 771, Oilseed residues Determination of moisture and volatile matter content ISO 5502, Oilseed residues Preparation of test samples 3 Terms and definitions For the purposes of

30、 this document, the following terms and definitions apply. 3.1 oil content sum of the mass fractions of the substances extracted under the operating conditions specified in this part of ISO 734 on the basis of the product as received NOTE 1 The mass fraction is expressed as a percentage. NOTE 2 On r

31、equest, the oil content may be expressed relative to dry matter. 4 Principle The test portion is ground in a micro-ball mill in the presence of a solvent and subsequently extracted with the same solvent in a suitable apparatus. The solvent is removed from the extract by distillation, then the residu

32、e is weighed after drying. BS EN ISO 734-2:2010Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-ISO 734-2:2008(E) 2 ISO 2008 All rights reserved5 Reagents and materials Use only r

33、eagents of recognized analytical grade, unless otherwise specified. 5.1 Technical hexane, n-hexane or light petroleum ether, essentially composed of hydrocarbons with six carbon atoms, of which less than 5 % distils below 50 C and more than 95 % distils between 50 C and 70 C. For either solvent, the

34、 residue on complete evaporation shall not exceed 2 mg per 100 ml. The solvent recovered from the extract by distillation should not be used for further determinations. 5.2 Cotton wool, fat-free. EXAMPLE Defatted cotton wool for ophthalmic use. 6 Apparatus Usual laboratory apparatus and, in particul

35、ar, the following. 6.1 Analytical balance, capable of being read to the nearest 0,001 g. 6.2 Drying oven, capable of being maintained at 103 C 2 C. 6.3 Mechanical microgrinder, capable of wet grinding in the presence of light petroleum ether and reducing the test portion to an average particle size

36、of less than 10 m. A commercially available ball mill1) is suitable. When using grinding beakers made of polytetrafluoroethylene, cooling is obligatory. A suitable cooling jacket is shown in Figure 1. Figure 1 Grinding beaker with cooling mantle 6.4 Funnel, made of light petroleum ether-resistant pl

37、astic, with a diameter of 70 mm, a stem external diameter of 10 mm and a stem length of 100 mm to 150 mm. The stem shall just reach into the extraction thimble on placing the funnel in the extraction apparatus as described in 9.3.4. There is a fixed metal pin in the funnel to retain the stainless st

38、eel balls (see Figure 2). 1) The Dangoumau ball mill is an example of a suitable product available commercially and has been studied in a ring test (Annex A). This information is given for the convenience of users of this part of ISO 734 and does not constitute an endorsement by ISO of this product.

39、 Equivalent products may be used if they can be shown to lead to the same results. BS EN ISO 734-2:2010Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-ISO 734-2:2008(E) ISO 2008

40、All rights reserved 3Dimensions in millimetres Figure 2 Funnel with stanced metal pin 6.5 Continuous extraction apparatus 2), with ground joints, consisting of a flat bottomed flask of capacity 100 ml to 200 ml, a connecting tube (extractor) to hold the extraction thimble, and a reflux condenser. Th

41、e apparatus should include a suitable heating element for boiling technical hexane under reflux conditions. This might be a water bath, steam bath or safety-approved electrical heating unit. NOTE The use of other extractors is conditional upon the results of a test on a standard material of known oi

42、l content to confirm the suitability of the apparatus. 6.6 Extraction thimbles, of dimensions 25 mm 100 mm, of wall thickness 1,5 mm and a reinforced bottom part. 7 Sampling A representative sample should have been sent to the laboratory. It should not have been damaged or changed during transport o

43、r storage. Sampling is not part of the method specified in this part of ISO 734. A recommended sampling method is given in ISO 55001. 8 Preparation of test sample Prepare the test sample in accordance with ISO 5502. 2) The Twisselmann and Butt extractors are examples of suitable products available c

44、ommercially. This information is given for the convenience of users of this part of ISO 734 and does not constitute an endorsement by ISO of these products. The Butt-type extractor is described in AOCS Official method Aa 4-38 4. BS EN ISO 734-2:2010Copyright European Committee for Standardization Pr

45、ovided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-ISO 734-2:2008(E) 4 ISO 2008 All rights reserved9 Procedure 9.1 General If it is required to check whether the repeatability limit (11.2) is met, carry out two single determinations

46、 in accordance with 9.2 to 9.3.11. 9.2 Test portion Weigh, to the nearest 0,001 g, 5 g of the well-mixed test sample in an extraction thimble (6.6). 9.3 Determination 9.3.1 Transfer the contents of the thimble to the beaker or tube of the mechanical microgrinder (6.3) containing all stainless-steels

47、 balls. 9.3.2 Add about 20 ml of solvent (5.1). Close the grinding beaker or tube and shake in the microgrinder (6.3) for 10 min or shake the stainless steel tubes horizontally on a flat bed shaker for 45 min horizontally at a frequency of 240 min1, with a horizontal displacement of 35 mm. 9.3.3 Pla

48、ce the thimble (6.6) in the connecting tube (extractor) of the extraction apparatus (6.5) and connect this to a dried and weighed flask. 9.3.4 Place the funnel (6.4) in the connecting tube in such a manner that the stem of the funnel reaches into the upper quarter of the thimble. 9.3.5 Then pour the contents of the grinding beaker through the funnel into the extraction thimble in such a manner that the balls remain on the metal pin in the funnel. Carefully rinse the grinding beaker, its lid and the balls with solvent in order to transfer all sample particles