1、BSI Standards PublicationBS EN ISO 11133:2014Microbiology of food, animalfeed and water Preparation,production, storage andperformance testing of culturemediaBS EN ISO 11133:2014Incorporating corrigendum November 2014BS EN ISO 11133:2014 BRITISH STANDARDNational forewordThis British Standard is the
2、UK implementation of EN ISO 11133:2014. It supersedes DD CEN ISO/TS 11133-1:2009 and DD CEN ISO/TS 11133-2:2003+A1:2011 which are withdrawn.The UK participation in its preparation was entrusted to Technical Committee AW/9, Microbiology.A list of organizations represented on this committee can be obt
3、ained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2014.Published by BSI Standards Limited 2014ISBN 978 0 580 66708 4ICS 07.100.30Compliance wi
4、th a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 May 2014.Amendments/corrigenda issued since publicationDate Text affectedBS EN ISO 11133:2014 BRITISH STANDARDISBN 978 0 58
5、0 88986 8Date Text affected30 November 2014 CEN Correction Notice 14 November 2014 implements ISO corrected text 1 November 2014: See ISO foreword for changesEUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 11133 May 2014 ICS 07.100.30 Supersedes CEN ISO/TS 11133-1:2009, CEN ISO/TS 11133-2:2
6、003English Version Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media (ISO 11133:2014, Corrected version 2014-11-01) Microbiologie des aliments, des aliments pour animaux et de leau - Prparation, production, stockage et essais de p
7、erformance des milieux de culture (ISO 11133:2014, Version corrige 2014-11-01) Mikrobiologie von Lebensmitteln, Futtermitteln und Wasser - Vorbereitung, Herstellung, Lagerung und Leistungsprfung von Nhrmedien (ISO 11133:2014, korrigierte Fassung 2014-11-01) This European Standard was approved by CEN
8、 on 20 March 2014. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards m
9、ay be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified
10、 to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Icelan
11、d, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Managem
12、ent Centre: Avenue Marnix 17, B-1000 Brussels 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 11133:2014 EBS EN ISO 11133:2014EN ISO 11133:2014 (E) 3 Foreword This document (EN ISO 11133:2014) has been prepared by Technica
13、l Committee ISO/TC 34 “Food products” in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorseme
14、nt, at the latest by November 2014, and conflicting national standards shall be withdrawn at the latest by November 2014. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identif
15、ying any or all such patent rights. This document supersedes CEN ISO/TS 11133-2:2003, CEN ISO/TS 11133-1:2009. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
16、 Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey
17、and the United Kingdom. Endorsement notice The text of ISO 11133:2014 has been approved by CEN as EN ISO 11133:2014 without any modification. EN ISO 11133:2014 (E) 3 Foreword This document (EN ISO 11133:2014) has been prepared by Technical Committee ISO/TC 34 “Food products” in collaboration with Te
18、chnical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by November 2014, and conflicting national s
19、tandards shall be withdrawn at the latest by November 2014. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes C
20、EN ISO/TS 11133-2:2003, CEN ISO/TS 11133-1:2009. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, F
21、ormer Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 111
22、33:2014, Corrected version 2014-11-01 has been approved by CEN as EN ISO 11133:2014 without any modification. EN ISO 11133:2014 (E) 3 Foreword This document (EN ISO 11133:2014) has been prepared by Technical Committee ISO/TC 34 “Food products” in collaboration with Technical Committee CEN/TC 275 “Fo
23、od analysis - Horizontal methods” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by November 2014, and conflicting national standards shall be withdrawn at t
24、he latest by November 2014. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes CEN ISO/TS 11133-2:2003, CEN ISO/
25、TS 11133-1:2009. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Maced
26、onia, France, Germany Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 11133:2014, Corrected version 2014-1
27、1-01 has been approved by CEN as EN ISO 11133:2014 without any modification. ISO 11133:2014(E) ISO 2014 All rights reserved iiiContents PageForeword vIntroduction vii1 Scope . 12 Normative references 13 Terms and definitions . 23.1 General terms and definitions . 23.2 Terminology of performance test
28、ing . 23.3 Terminology of culture media 33.4 Terminology for test microorganisms 64 Quality assurance management . 74.1 Documentation . 74.2 Storage . 84.3 Laboratory preparation of media . 84.4 Storage and shelf-life of prepared media 114.5 Preparation for use . 124.6 Incubation of solid media in P
29、etri dishes 144.7 Disposal of media . 145 Test organisms for performance testing.145.1 General 145.2 Selection of test organisms 145.3 Preservation and maintenance of test organisms .155.4 Microorganisms for performance testing .166 Quality control and performance testing of culture media 196.1 Gene
30、ral requirements 196.2 Physical and chemical quality control . 196.3 Microbiological quality control 196.4 General requirements for microbiological performance testing 206.5 Performance evaluation and interpretation of results 216.6 Confirmation media and reagents . 227 Methods for performance testi
31、ng of solid culture media .227.1 General 227.2 Methods for quantitative tests 227.3 Testing of culture media used for membrane filtration 247.4 Methods for qualitative tests . 248 Methods for performance testing of liquid culture media.258.1 General 258.2 Quantitative tube method for performance tes
32、ting of liquid enrichment media (dilution to extinction method) . 258.3 Qualitative tube method for performance testing of selective liquid media 268.4 Qualitative single tube method (turbidity) for performance testing of liquid media .279 Methods for performance testing of diluents and transport me
33、dia 289.1 General 289.2 Method for testing diluents . 289.3 Method for testing transport media 2910 Documentation of test results .3010.1 Information provided by the manufacturer 3010.2 Traceability . 30Annex A (informative) Designation of the components of culture media in International Standards o
34、n microbiological analysis of food, animal feed and water 31BS EN ISO 11133:2014ISO 11133:2014(E)iv ISO 2014 All rights reservedAnnex B (normative) Preparation of reference stock and working culture .33Annex C (normative) Flow charts of methods for performance testing .38Annex D (informative) Exampl
35、e of card for recording test results of culture media 42Annex E (normative) Test microorganisms and performance criteria for culture media commonly used in food microbiology .44Annex F (normative) Test microorganisms and performance criteria for culture media commonly used in water microbiology66Ann
36、ex G (normative) Use of control charts to monitor quantitative testing of solid culture media 78Annex H (informative) Quality assurance of culture media Troubleshooting 85Annex I (informative) Quantitative testing of liquid media .87Annex J (normative) Definition of microbiological performance tests
37、 for standardized culture media 91Bibliography .95BS EN ISO 11133:2014ISO 11133:2014(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out th
38、rough ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collab
39、orates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval
40、 criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2. www.iso.org/directivesAttention is drawn to the possibility that some of the elements of this document may be the subject of
41、patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received. www.iso.org/patentsAny trade name used in
42、 this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barrier
43、s to Trade (TBT) see the following URL: Foreword - Supplementary informationThe committee responsible for this document is ISO/TC 34, Food products, Subcommittee SC 9, Microbiology, in collaboration with Technical Committee ISO/TC 147 Water quality, Subcommittee SC 4, Microbiological methods.This fi
44、rst edition of ISO 11133 replaces the second edition of ISO/TS 11133-1 (ISO/TS 11133-1:2009) and the first edition of ISO/TS 11133-2:2003, which have been technically revised. It also incorporates the Amendment ISO/TS 11133-2:2003/Amd.1:2011. In particular, it also includes requirements for microbio
45、logy media for water testing. It supersedes ISO 9998:1991.This corrected version of ISO 11133:2014 incorporates the following corrections: In Annex ESelective media for enumeration of microorganisms DG18 column Incubation: d was replaced with days; EC column control strain: was deleted after Pseudom
46、onas; mCCDA: dwas deleted after both species of Campylobacter; bwas added after 000156; mCCDA: the criteria “Total or partial inhibition (0-1)” was added to the control stain E. coli and “Total inhibition (0)” was added to S. aureus; TSC: the row with Pseudomonas aeruginosa and the WDCM number 00025
47、 was deleted.Selective enrichment media Bolton productivity: the cocktails of control strains were split into 2 separate cells; EE: dwas added before i, after both stains of Salmonella; ITC: a new cocktail of strains was introduced for Productivity; PBS selectivity: bwas added after 00025; ISO 2014
48、All rights reserved vBS EN ISO 11133:2014ISO 11133:2014(E) RVS Productivity: added dto E. coli. Non-selective liquid media mCCDA: dwas deleted after both species of Campylobacter; bwas added after 000156; mCCDA column Characteristic reactions: “colonies” was added after “moist”; PEMBA lane productiv
49、ity: iwas deleted after “good growth (2)”; Media TCBS was added after TBX; VRBG: one Salmonella Typhimurium was replaced by Salmonella Enteritidis WDCM 00030 and d,iwas added to both Salmonella;Non-selective isolation media Nutrient agar: the WDCM numbers were inverted between S. Typhimurium and S. Enteritidis; TSYEA: name and WDCM were corrected to Listeria monocytogenes 4b WDCM 00021b;Multipurpose media Pre-enrichment for Enterobacteriaceae: added dto both Salmonella and deleted “or” between the 2 WDCM numbers.Reference media for enum
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