GOST 31643-2012 Juice products Determination of ascorbic acid by High Performance Liquid Chromatography (HPLC) method《果汁产品 高效液相色谱(HPLC)方法测定抗坏血酸》.pdf

1、 , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)316432012, - 1.092 . 1.22009 . , . , , , - 1 () - ( ), ( )2 3 , (- 50 20 2012 .) : ( 3166) 00497 ( 3166) 00497 AZAMBYKZKGMDRUTJ -4 26 - 2012 . 437- 316432012 - 1 2013 .5 5369320096 ( ) - . - , -. , 2013 -, II 316432012 Juic

2、e products. Determination of ascorbic acid by High Performance Liquid Chromatography (HPLC) method 2013 07011 , , - , , , , - ( ) () . ( ) 5 1000 /3(1) .2 : 5725-12002 ( ) -. 1. 5725-22002 ( ) -. 2. 12.1.00588 . - 12.1.00776 . . 12.1.01893 . - . 12.1.01979 . . - 24576 . 2-. 177074 ( 104283, 478880)

3、. -, , , . 655280 . . 670972 . 1202676 . 2296790 ( 248680, 339982) - . 241042001 . 1 316432012 2533682 . , 2631384 . , - 2667185 , -. 2922791 ( 835-181) . . 1. - - , 1 - , , - . (), () . , -, , , .3 - - (). 243 .4 , , , ,4.1 (- 200 600 ) - .4.2 : 200 800 ; c177 0,2 ; c177 0,08 (c108 = 656 ); c177 0,

4、002 .1) 0,3 . c177 0,003 . 1 .; c177 0,0008 . 1 .; 2) - c177 0,0001 . 0 ., c108 = 500 c177 0,0006 . 1 ., c108 = 500 ; 1,0 % (c108 = 200 ) 0,05 % (c108 = 220 340 ).4.3 250 4,6 , -, 5 (RP 18).4.4 24104 - c177 0,1 .4.5 1-2-1, 1-2-2, 1-2-5, 1-2-1 1-2-25 2- 29227.4.6 25, 100 250 .4.7 2- 1770: 50 1000 3,

5、25, 50, 100, 500 1000 3, 10 203.4.8 () 2 6 3.4.9 .1). .2) .2 3164320124.10 0,20 0,45 .4.11 .4.12 5 3 22967.4.13 (g-) 800 1000.4.14 - ( ).4.15 (pH-) c177 0,01 . pH.4.16 12026.4.17 6709.4.18 1.4.19 99,0 %.4.20 () 245, .4.21 6552.4.22 25336: , 50, 100 1000 3. , , - - , .5 26313, 26671.6 6.1 25336 100 3

6、 15,6 245 , 80 3 6709, 1770 - 1000 3, . - 1000 3, pH 6552 2,5, 4.15. 4.9 15 , 0,45 . 3 .6.2 6.2.1 - , , . - , , - 4.13 990 g 15 . 1 2 3 4.12 , 0,45 0,20 4.8.6.2.2 - 10,0 % ( , , -, .) , 4.13 - 990 g 15 . 10,0 % ( , -, .) 1:5 - . 1770 50 3 1770 10 3 . -, , -3 316432012 990 g 15 . 990 g 15 . 1 2 3 , - 0,45 0,20 .6.2.3 - 1:5. 24104 50 3 4.7 - 5 7 , 25 35 . (), . , , 6.2.2. 1 2 3 , - 0,45 0,20 .6.2.4 6.2.1, 6.2.2 - , 6.2.3 .6.3 - , - , 1 1. . 1 /- , ,/ (/)11()100 0,030 - 50 3 - , - 40 3 , - 100 3,