GOST 31710-2012 Milk and mik-based products Detection of themrmoneuclease produced by coagulase-positive staphylococci《奶和奶基制品 利用凝固酶阳性葡萄状球菌产生耐热核酸酶的测定》.pdf

1、 , (ISO 8870:2006, MOD) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)317102012(ISO 8870:2006), - 1.092 . - 1.22009 . , . -, , , 1 - -,42 3 , (- 1 2012 . 51) : ( 3166) 00497 ( 3166) 00497 BYKZKGMDRUTJ -4 29 2012 . 1770- 317102012 - 1 2013 .5 ISO 8870:2006 Milk and mik-b

2、ased products Detection of themrmoneuclease produced bycoagulase-positive staphylococci ( . , ) (, - , ), . (MOD). 5283220076 ( ) - . , - . - , 2013 -, II 3171020121 12 13 14 .15 .16 37 .38 39 .410 .5III 317102012 , Milk and milk-based products.Detection of themrmoneuclease produced by coagulase-pos

3、itive staphylococci 201307011 , - . - .2 : ISO 721811 . 922584 . 2680986 . , - - , 1 , , . (), (-) . , , , , .3 :3.1 (thermonuclease): , -,() , - .4 , .5 5.1 . 9225.1 317102012(ISO 8870:2006) , , - , . , , , 0 5 1 , .5.2 - 5.2.1 -5.2.1.1 :() (C4H11NO3) 6,06 ; (CaCl2) 0, 11 ; 1000 3.5.2.1.2 () 9803.

4、9,0 1000 3.5.2.2 5.2.2.1 : () 0,3 ; (NaCl) 10,0 ; 10,0 ;- (. 5.2.1) 1000 3.5.2.2.2 (.6.2)(1,5).5.2.3 5.2.3.1 : (C15H16N3SCl)a)0,31; 10 3.5.2.3.2 (. 6.12).5.2.4 5.2.4.1 : (. 5.2.2) 1000 3; (. 5.2.3) 3 3.5.2.4.2 (50 c177 1) , . 0 5 .5.2.5 (. 6.8) 13 3 (. 5.2.4) , (50 c177 1) . . 2 ( -) 0 5 .5.3 , (HCL

5、) = 2 /3.5.4 , (NaOH) = 2 /3.5.5 , (CCl3COOH) = 3 /3. 49,02 100 3.5.6 . , , .5.7 - (BHI )5.7.1 : 10,0 ;2 317102012a) ( : C.I. 17; C.I. No. 52040). 12,5 ; 5,0 ; 2,0 ; 5,0 ; , (Na2HPO4)2,5; 1000 3.5.7.2 , .,- 7,4 c177 0,2 25 . 5 3 (. 6.11). (121 c177 1)15.5.8 - ( ) Staphylococcus aureus, - .6 , ISO 72

6、18, , :6.1 , (37 c177 1) .6.2 ,(50 c1771),( ).6.3 27 000 1.6.4 2 800 1.6.5 2 - .6.6 0,01 3.6.7 - 3 .6.8 , , 90 100 .6.9 10, 100 1000 3.6.10 - .6.11 15 100 .6.12 . . .7 , . , 26809. , .8 , 3,8.- . , 8,5 -.15- - .3 3171020128.1 8.1.1 100 3 (. 5.3) 3,8.8.1.2 20 5 (. 5.6) 50 3. (. 5.3) 3,8.- . , , , .,-

7、.- .201003(45).-(6/)4,5.7503. , 12,5 . (16 ) (25 c177 1) . 4,5. . 100 3 8.1.3.,20403.- (. 5.3) 3,8.8.1.3 8.1.3.1 (. 6.3) 27000 1 34000 1520.8.1.3.2 . 0,05 3 (. 5.5) 13 .8.1.3.3 27000 1 34000 1 5 20 .8.1.3.4 13-(.5.2.1).8,5- (. 5.4). - 2 3, .8.1.3.5 (. 6.2) 15 .8.2 - ( )8.2.1 -(.5.8)BHI(.5.7).24(37c1771).-(.6.4)280013500115. .8.2.2 (. 6.2) 15 . 5 . Staphylococcus aureus (, ) , - -.8.3 - (.6.5)(.5.2.5). (. 6.1) 60 (37 c177 1) . 10 .8.4 8.4.1 103(.8.2.2),103 (. 8.1.3.5).8.4.2 (. 6.1) 4 (37 c177 1) . 24 .9 ( ) 1 ()., , .4 317102012 - 106/ , . - , . , , , .10 :- , ;- ;- ;- , ,