GOST 31744-2012 Microbiology of food and animal feeding stuffs Clostridium perfringens colony-count technique《食品和动物饲料的微生物学 产气荚膜杆菌菌落计数技术》.pdf

1、 , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC)31744null2012(ISO 7937:2004) Clostridium perfringens(ISO 7937:2004, MOD) 317442012II, 1.092 . 1.22009 . , - . , , -, 1 - ( ) . . 2 ()3 , (- 15 2012 . 42) : ( 3166) 00497 ( 3166) 00497 BY KZ KG MD - RU TJ UZ 4 29 - 2012 . 1

2、766- 317442012 (ISO 7937:2004) - 1 2013 . 5 ISO 7937:2004 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of Clostridium perfrin-gens Colony-count technique ( . - lostridium perfringens. ). , , . ISO/T 34 - (ISO). (en). , - , , , (-) . (MOD). 534002009 ( 7937:200

3、4)6 317442012III , 2014 -, - - , . () - . , - - 317442012IV1 12 13 .14 .25 , 26 .67 68 69 610 .811 .10 () 11 141 201307011 Clos-tridium perfringens. , - , , - .2 : ISO 72182011 . - ISO 11133-12011 . - . 1. ISO 11133-22011 . - . 2. ISO 161402011 . 2666985 . , 1 , - . (), (-) . , , , , .3 :3.1 lostrid

4、ium perfringens (. perfringens): , (- , , 317442012(ISO 7937:2004) Clostridium perfringensMicrobiology of food and animal feeding stuffs.Clostridium perfringens colony-count technique 3174420122) , , .3.2 . perfringens: - lostridium perfringens 3 - , .4 4.1 , , . - . ( ) .4.2 37 (20 2) .4.3 .4.4 . p

5、erfringens 3.5 , . 1, ISO 7218, ISO 11133-1 ISO 11133-2 , , .5.1 ISO 7218. 5.2 - (SC)5.2.1 1. 1 , 15,0 , 5,0 , 5,0 (Na2S2O5), 1,0 (III) , 1,0, 9,0 18,0 b, 31000 15 % ( ).b .5.2.1.1 . , (7,6 0,2) 25 . . (6.1) 15 121 . (5 3) . 5.2.2 D-5.2.2.1 2. 3174420123 2 D-, 4,0, 3100 .5.2.2.2 D- . (3 2) -. 5.2.3

6、(. 9.2) 1 3 D- (5.2.2) 100 3(5.3.1), 44 47 .5.2.4 (SC) ISO 11133-1. - ISO 11133-2 ( .1).5.3 5.3.1 3. 3 , 10,0L-, 0,5D - , 5,5 , 5,0 , 2,5 (), 0,5, 0,52,0 , 0,001, 31000 .5.3.2 , . - , (7,3 0,2) 25 . 10 3 16160 15 121 . .5.3.3 ISO 11133-1. - ISO 11133-2 ( .4).5.4 - (LS) ()5.4.1 5.4.1.1 4. 3174420124

7、4 , 5,0 , 2,5 , 2,5, 10,0L- , 0,3D-, 5,5, 310005.4.1.2 , . , (7,1 0,2) 25 . 8 3 15 121 . (3 2) -.5.4.2 5.4.2.1 5. 5 (Na2S2O5) , 1,2, 31005.4.2.2 . .5.4.3 (III) 5.4.3.1 6. 6 (III) , 1,0, 31005.4.3.2 (III) . .5.4.4 -. , . 8 3 (5.4.1) 0,5 3 (III) (5.4.3) 0,5 3 (5.4.2). .5.5 ()5.5.1 7. 3174420125 7 , 5,

8、0 , 3,0, 5,0, 5,0 (KNO3) , 1,0 (Na2HPO4), 2,5, 1,05,0 , 0,001, 31000 .5.5.2 , . , (7,3 0,2) 25 . - 10 3 - 15 121 . , (5 3) . 15 .5.6 ()5.6.1 5-2- (5-2-ANSA) 0,1 5-2-ANSA 100 3 15 % ( ) . - . ( ) (5 3) 5.6.2 0,4 100 3 15 % ( ) . . ( ) (5 3) 5.6.3 5.6.1 5.6.2 -. .5.7 ()5.8 - 5.8.1 8. 8 , 15,0 , 10,0, 10,0, 120,0 , 0,05, 31000 31744201265.8.2 ( ). , (7,5 0,2) 25. . 10 3 15 121 . , - (5 3) .