GOST 31772-2012 Propolis Antimicrobial activity《蜂胶 抗菌活性》.pdf

1、 , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)317722012, - 1.092 . - 1.22009 . , . , , -, 1 - - ( )2 (-)3 , (- 1 2012 . 51) : ( 3166) 00497 ( 3166) 00497 AMBYKZKGMDRUUZ -4 29 2012 . 1661- 317722012 - 12013 .5 5312420086 - , - . () - . , , 2014 -, II 317722012 Propolis.

2、 Antimicrobial activity 201307011 - - ( ) 100 600 /3 -. 28886.2 : 177074 ( 104283, 478880) . -, , , . 314584 . IS 5725-12003 ( ) -. 1. IS 5725-62003 ( ) -. 6. 596267 . 670972 . IS 72182011 . - 10444.184 . , , - , 241042001* . 2533682 . , 2562983 . 2849890 . . 2888690 . 2922791 ( 835-181) . . 1. * 53

3、2282008 . 1. . .1 317722012 - - - , 1 , - . (), (-) . , , , , .3 25629, ISO 5725-1, :3.1 : (minimal inhibitory concentration): - , - .4 28886 ( 3.1.1).5 - IIIIV , , , -.6 , , ,6.1 - c1770,001 24104.6.2 c1771 C - 0 C 70 C 28498.6.3 3145.6.4 0,02 0,2 3.6.5 3-10-2, 3-100-2, 3-1000-2 1770.6.6 5 3 29227.

4、6.7 (36 c177 1) C.6.8 100 150 , (25 c177 5) .6.9 .6.10 40 C 55 C c1772 C.6.11 -1-250-29/32, -1-10-14/23 25336.6.12 -75-110 25336.6.13 1-21-200 25336.6.14 25336, 100 - 90 100 .6.15 .6.16 ( ).6.17 6709.6.18 5962.6.19 .6.20 - .6.21 10444.1 ( 4.29).6.22 Staphylococcus aureus, 209p (FDA 209p, 6538P).2 31

5、77220126.23 1,0 MacFarland -10 (0,36 c177 0,01) . -.6.24 , - IS 7218. , -, . .7 7.1 70 %- 1000 3 1770 730 3 5962 6709 1000 3.7.2 - 250 3 25336 (5,0 c177 0,5) 100 370 %- , 7.1, 6.8 (24 c177 1) . - 6.16. () - 25 C.7.3 , , - ISO 7218 .7.4 - Staphylococcus aureus 6.22 (24 c177 2) (.6.19) (36 c177 1) C.

6、10444.1 - S. aureus, 109/3 (. 6.23). 10- - - 106/3.8 8.1 10 3 25336 , 5 3 29227 4 3, - 7.2. (45 c177 2) C (- 0,001 ). .8.2 2-21-200 25336 10 3 1770 10 3 - (), - 7.3, 6.10 (52 c177 2) C . 6.4 0,02; 0,04; 0,06; ; 0,20 3, 6.14. , , 0,1 3 - S. aureus, 7.3, 6.15. - ; - - 70 %- , . (36 c177 1) C (24 c177

7、1) .8.3 , - , 8.2 , -, 0,3 3.3 3177220129 9.1 , /3, = V c215101c215m c21541c215106, (1) V , - - 8.2, 3;101 , 3;m , 8.1, ;41 , 8.1, 3;106 . , 8.1, 0,243 . 8.2 - - 0,06 3 . (1) :i= 0,06 c215101c2150,243 c21541c215106= 364,5 (/3). 364,5 /3.9.2 , , r ISO 5725-6. r 1. r - . ( ) - , CR0,95(3), -. CR0,95(3) 1. 1 , /3 r, /3 CR0,95(3), /3R, /3 100 600 . 0,18 0,21 0,35 .9.3 , , R ISO 5725-6. . R 1.9.4 , , 100 600 /3 , , ( c177c68), /3, = 0,95, 9.2, /3;c177c68 10, /3. , 600 /3, 600 /3.10 , , P = 0,95, c177c68= c177 0,25.11 .4 317722012 638.16:006.354 65.140 52 :