GOST 32011-2013 Microbiology of food and animal feeding stuffs Horizontal method for the detection of Escherichia coli O157《食品和动物饲料微生物学 利用水平法进行大肠杆菌(Escherichia coli I157)的测定》.pdf

1、 Escherichia coli 157(ISO 16654:2001, MOD) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)320112013(ISO 16654:2001), - 1.092 . - 1.22009 . , . , , -, 1 - ( )2 ()3 , (- 7 2013 . 43) : ( 3166) 00497 ( 3166) 00497 KZKGMDRUTJUZ -4 27 2013 . 226- 320112013 (ISO 16654:2001) 12

2、014 .5 ISO16654:2001 Microbiology of food and animal feeding stuffs Horizontal method for the detection ofEscherichia coli 157 ( . - Escherichia coli 157) , , . SC 9 - ISO/TC 34 (ISO). (en). , - , , , (-) . (MOD).6 539132010 ( 16654:2001)7 - , -II 320112013 . () - . , , 2014 -, III 3201120131 12 13

3、24 .25 , 26 57 .68 69 , 610 .811 812 .8 () .9 10IV 320112013 Escherichia coli 157Microbiology of food and animal feeding stuffs.Horizontal method for the detection of Escherichia coli 157 201407011 , - Escherichia coli 157 - . .! Escherichia coli O157 , . . , , , . - - . .2 : IS 72182011 . - 2666885

4、 . 2666985 . - , 1 , - . - (), (). , , , - , .1 320112013(ISO 16654:2001) 3 ISO 7218, - :3.1 scherichia coli O157 (E. coli O157): , , 157.:1 - E. coli O157 CT-SMAC (5.2).2 - .4 Escherichia coli O157 - (. ):- , -, (mTSB + N), (41,5 c177 1) C 6 , 1218 ;- , E. coli O157;- - -, ( ) (CT-SMAC), ;- - CT-SM

5、AC , E. coli O157, E. coli O157. , - , .5 , ISO 7218.5.1 ( - (mTSB + N): .17,0 3,0D(+)- .2,5 No. 3 .1,5 5,0 (K2HPO4).4,0 .100035.1.1 , - . pH, pH- (. 6.6), , , pH (7,4 c177 0,2) 25 C. (6.7). 15 (. 6.1) 121 C.5.1.1.1 : 0,45 .100 : . .2 3201120135.1.1.2 (. 5.1.1) 1 3 4 3 - (. 5.1.1.1) 225 3, 900 3 mTS

6、B (. 5.1.1). 20 /3 mTSB.5.2 : - ( ) (CT-SMAC)5.2.1 : .17,0 3,0.10,0 No. 3 .1,5 5,0 0,03 .0,001 918 * .100035.2.1.1 , - . pH (. 6.6), , - (7,1 c177 0,2) 25 C, 15 (. 6.1) 121 C.5.2.2 : 0,25 100 3;5.2.2.1 . 1 , , , .5.2.3 : .5,0 .100,0 35.2.3.1 . , . (3 c177 2) C 1 .5.2.4 : (. 5.2.1)10003 (. 5.2.2) 1,0

7、3 (. 5.2.3) .1,035.2.4.1 (. 5.2.1) 44 C 47 C (. 6.5), , , 44 C 47 C. 1 3 1 3 1000 3 . - 15 3 (. 6.15). . 2,5 /3, 0,05 /3. , , 25 C 50 C (. 6.2), . 30 .* .3 320112013 (3 c177 2) C -.5.3 CT-SMAC Escherichia coli O157. (. 5.2.4.1). (3 c177 2) C , .5.4 : .3,0.5,0 918 * .100035.4.1 , . pH , (7,0 c177 0,2

8、) 25 C. (. 6.7) . 15 (. 6.1) 121 C.5.4.2 15 3, (. 5.4.1) 44 C 47 C (. 6.5) . (. 5.2.4.1). (3 c177 2) C -.5.5 / : 10,0 5,0DL- 1,0 .100035.5.1 , . pH (. 6.6) , - (7,5 c177 0,2) 25 C. 5 3 (. 6.7) . 15 (. 6.1) 121 C.5.6 :4-5,02-1-o -1-o.75,0 3 (c11420 1,18 /3 1,19 /3)25,0 35.6.1 4- , (. 6.5), 44 C 47 C. . - (3 c177 2) C. - -, .* .4 3201120135.7 , Escherichia coli O157 , E.