GOST 32249-2013 Juice products Determination of ethanol by enzymatic method《果汁产品 用于测定乙醇的酶法》.pdf

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2、zymatic ( 52:2005 , - IFU International Federationof Fruit Juice Produces6 - , - . () - . , , 2015 -, II 3224920131 12 13 , .24 25 , , , , 26 37 .38 .59 610 , . . 811 .9III 322492013 Juice products. Determination of ethanol by enzymatic method 201507011 , , , , , , . . ( ) . ( ) 50 50000 /3(1) .2 :

3、12.0.00490 . - . 12.1.00491 . . 12.1.00588 . - 12.1.01979 . . - OIML R 76-12011 . - . 1. 177074 ( 104283, 478880) . -, , , . 311877 . . 432877 . . 5725-62003 ( ) -. 6. 670972 . 1202676 . 1830087 . 2533682 . , 2631384 . , 1 322492013 2667184 , . 2922791 ( 835-181) . . 1. - - , 1 , . (), (-) . , , , ,

4、 .3 , 3.1 :3.1.1 , () , 25 1 .3.2 (NAD) , . (NADH) , . (ADH) . (Al-DH) .4 - : (ADH) : + NAD+2ADHc190c174c190c190 + NADH + H+ (Al-DH) - (NAD+) : + NAD+2OAl-DHc190c174c190c190c190 + NADH + H+ ( ) - AH, , :334, 340 365 .5 , , , ,5.1 , 334, 340 365 c1771%, c1770,3 .5.2 - c1770,05 . .5.3 OIML R 76-1 c -

5、c1770,001 .5.4 -2 0 +150 c1771 .5.5 - 0,2 .5.6 5 20 2 10 3.5.7 0,20 0,45 47 - .5.8 (g-) 1000.5.9 400 1200 1.5.10 .5.11 :- 2-50-2 2-1000-2 1770;2 322492013- 2-50-2, 2-100-2 2-1000-2 1770;- 1-2-1-1, 1-2-1-5, 1-2-1-25 29227;- -2-20-14/23 1770.5.12 100 1000 3 - c1771% 28311.5.13 25336:- ;- 100, 400 1000

6、 3;- -2-15-160.5.14 12026.5.15 6709 .5.16 2 5 - .5.17 427,.5.18 , . . 3118, 1 /3.5.19 , -Na2, 98 %.5.20 (), , 75 .5.21 (), , 300 .5.22 C 18300 96,2 %.5.23 1), :- 1100 3 9,0;- 230 , 4,0 0,8 ;- 31,6 3 (ADH) 7000 ;- 4 ( 5060 /3.- ).5.24 4328, . .5.25 (1202) 95 %.5.26 . , , , .6 6.1 26313, 26671.7 7.1 7

7、.1.1 1 ( ) 1 (. 5.23) , . 1 20 25 . 1 4 .7.1.2 2 () 2 2 (. 5.23) 3 3 1 (. 5.11) , . 2 4 .7.1.3 3 () 3 (. 5.23) . 3 4 .1) R-Biopharm GmHb, Roche Diagnostics Megazyme . - .3 3224920137.1.4 4 ( ) 4 (. 5.23) . . . , - , .7.2 7.2.1 0,30 /3, 9,0 . 5,0 40 3 (). (9,0 c177 0,1) . (. 5.18). - 50 3 . 4 2 .7.2.2 () - 49 /3 103 -Na2(. 5.19) 3 3- . 4 28 .7.2.3 () (. 5.20) 0,5 3 7513. 4 8 , 20 .7.2.4 () 0,5 3 (. 5.21) 25 0,5 3 . 4 6 , 4 .7.2.5 500 3 (. 5.4) 500 (. 5.22). - . , . - . 1003 10 3 - . , , . 6 3 .7.3 7.3.1