GOST 32372-2013 Testing of chemicals of health hazard In vitro 3T3 NRU phototoxicity test《健康危险化学品测试 体外3T3 NRU光毒性试验》.pdf

1、 , () INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) 32372 2013 In vitro 3T3 NRU (OECD, Test 432:2004, IDT) 2014 32372 2013 II , 1.092 . 1.22009 . , . , , 1 ( ), - , , ( ) 2 3 , ( 442013 14 2013.) : ( 3166) 004 97 ( 3166) 004 97 AM KZ KG MD RU TJ - 4 OECD Test 432:2004 In

2、vitro 3T3 NRU phototoxicity test ( 432 In vitro 3T3 NRU ). (en). (IDT) 5 22 2013 . 773- 323722013 1 2014 . 6 , . () . , . 323722013 III , 2014 , 32372 2013 IV , . . ( ), . , / , 10 -1 -1 . , , in vitro 3T3 NRU 1. in vitro 3T3 NRU in vivo . , , : , , . , . in vitro , in vivo in vitro. , . 323722013 1

3、 In vitro 3T3 NRU Testing of chemicals of health hazard In vitro 3T3 NRU phototoxicity test 20140801 1 In vitro 3T3 NRU. , . , . , , in vivo . 2 : 2.1 (UV light wavebands): , () : ( 315 400 ), ( 280 315 ) ( 100 280 ). ; 320 , -1 -2 340 . 2.2 (Cell viability): , (, (Neutral red) ), / . 2.3 : , , . 2.

4、4 (Irradiance): , , /2 /2. 2.5 (Relative cell viability): , (), (+ Irr, - Irr), . 2.6 (Dose of light): ( ) () , , ( ) , : /2 /2. 2.7 (Phototoxicity): , , . 2.8 Balb/c 3T3 : (ATCC) (ECACC). 2.9 IC50: , 50 %. 32372 2013 2 2.10 MPE ( ): , , (-Irr) (+Irr) / . 2.11 PIF ( ) (Photo-Irritation-Factor): , (I

5、C50) , (-Irr) (+Irr) / . , 1 , . (), () . , , , , . 3 In vitro 3T3 NRU . , 24 . , . , . , , , . Balb/c 3T3 24 . 96 . , . 24 . . , , IC50 , . 4 4.1 , Balb/c 3T3, 31 , . , , . . Balb/c 3T3 , 100, . 4.2 . Balb/c 33 DMEM ( ), 10 % , 4 323722013 3 , (100 ), (100 /), 37 , 5,0 % 7,5 % 2 . . 4.3 , . ( , 48 ). Balb/c 3T3 , 96-, 1104 . 96-, , , (+Irr), (-Irr). 4.4 . , , . , , (EBSS) , , , (, ) . , 50 2 . , EBSS, 7,5 % 2. 5 % 2, . , , . , , ( ), , . , . (DMSO), (EtOH) . . , , / .