GOST 33163-2014 Juice products Detection of Alicyclobacillus bacteria species《果汁产品 脂环酸芽孢杆菌菌种的检测》.pdf

1、 , () INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) 33163 2014 Alicyclobacillus 331632014 II , 1.092 - . 1.22009 . -, . , , , 1 () 2 3 , ( 5 2014 . 462014) : ( 3166) 00497 ( 3166) 00497 AZ AM KG RU TJ UZ UA 4 25 2015 . 160- 331632014 01 2016 . 5 12:2004 (Rev. 2007) Alicyc

2、lobacillus, ( ) IFUMB Nr. 12 (Rev. 2007) Method on the Detection of Taint Producing Alicyclobacillus in Fruit Juices (International Federation of Fruit Juice Produces) 6 , . () . , , 2015 , 331632014 1 Alicyclobacillus Juice products. Detection of Alicyclobacillus bacteria species 2016 01 01 1 , , ,

3、 , , ( ), , Alicyclobacillus: - ; - ; - . Alicyclobacillus ( ). 2 : 177074 ( 104283, 478880) . , , , . 249375 . 3-. 311877 . . 365269 . . 419875 . . 423377 . . 432877 . . 670972 . ISO 72182011 . 928475 . 10444.184 . , , , 1092976 . . ISO 11133-12011 . . 1. ISO 11133-22011 . . 2. / 170252009 33163201

4、4 2 1830087 . * 2533682 . , 2666985 . 2667091 . 2922791 ( 835-181) . . 1. 3042597 . 319042012 . , 1 , . (), () . , , , , . 3 : 3.1 Alicyclobacillus: , - , , -, , - , . : 1 Alicyclobacillus : A. acidoterrestris, A. acidocaldarius, A. hesperidum . 2 6 . pH 16 C 70 C. 2 Alicyclobacillus . 3.2 Alicyclob

5、acillus: Alicyclobacillus . 3.3 Alicyclobacillus: () Alicyclobacillus, 1 1 3 , , . 3.4 (2-): Alicyclobacillus, , , . 4 4.1 Alicyclobacillus , . 4.2 Alicyclobacillus (. 5) 3,8 . . 4.3 Alicyclobacillus, () Alicyclobacillus Alicyclobacillus 1 1 3 ( ). 4.4 Alicyclobacillus, , 65 C. Alicyclobacillus, 65

6、C , . 4.5 Alicyclobacillus , , . * 55878-2013 . . 331632014 3 Alicyclobacillus , , , . 5 5.1 ISO 7218, ISO 11133-1 IS 11133-2. 5.2 Alicyclobacillus : YSG ( ), BAT (Alicyclobacillus Agar), Alicyclobacillus. K- ( 45 C) A. acidoterrestris , , A. acidocaldarius A. acidiphilus. . 5.3 4328, 100 /3, 10444.

7、1. 5.4 3118, 36,5 /3, 10444.1. 5.5 , 10444.1. 5.6 6709. 5.7 18300. 5.8 4233. , , 5.1 5.2, - , , . 6 , , 6.1 ( ) (), , 3 . 6.2 500 , 0,1 . 6.3 . 6.4 , 80 1 . 6.5 , (45 5) . 6.6 , 9001000 , - . 6.7 - 0,1 . 25 . 6.8 , 0 100 1 . 6.9 0,22 . 6.10 - 3 , 10 3 . 6.11 90 120 , . 6.12 250 500 3 25336. 6.13 -2-16-150 25336. 6.14 2- 29227 1, 2, 5 10 3. 6.15 2- 50, 100 1000 3 2 2 1770. 6.16 2- 1770. 6.17 50, 100 1000 3 25336. 6.18 9284. , , 331632014 4 . . 7 31904, 26669 : 50 (3), . , , Alicyclobacillus , , 45 3 . , , . , , . , , . 8 Alicyclobacillus 8.1 8.1.1