GOST EN 12822-2014 Foodstuffs Determination of vitamin E (alpha- beta- gamma- and delta-tocopherols) content by high performance liquid chromatography《 食品 采用高效液相色谱法测定维生素E (α- β- γ-.pdf

1、 , () INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) EN 12822 2014 (-, -, - -) (EN 12822:2000, IDT) 2015 EN 128222014 II , - 1.0 92 . 1.22009 . -, . , , , 1 - ( ) , 4 2 ( 335) 3 , (- 30 2014 . 67-) : ( 3166) 004 97 ( 3166) 004 97 AM BY KG MD RU TJ - 4 19 - 2014 . 900- EN 1

2、28222014 - 1 2016 . 5 N 12822:2000 Food-stuffs Determination of vitamin E by high performance liquid chromatography Measurement of -, -, - and -tocopherols ( . . -, -, - -). (de). , - , . - . (IDT) 6 - , . () - - . , , 2015 -, - EN 12822 2014 1 (-, -, - -) Foodstuffs. Determination of vitamin E (-,

3、-, - -tocopherols) content by high performance liquid chromatography 2016 01 01 1 - (). -, -, - -. 1, 2, 3. 2 . , , - . - , . EN ISO 3696 Water for analytical laboratory use Specification and test methods (ISO 3696:1987) ( . ) EN ISO 5555 Animal and vegetable fats and oils. Sampling (ISO 5555:1991)

4、( - . ) - , 1 , - . (), - () . , , , , . 3 -, -, - - - () ( ) , , . -. - (4 - 13). , - , . 4 , , , 1 EN ISO 3696. EN 128222014 2 4.1 . 4.2 , (C2H5OH) = 100 %. 4.3 , (C2H5OH) = 96 %. 4.4 , . 4.5 () = 50 /100 3 60 /100 3 - () = 28 /100 3 9:1. 4.6 , (), , , (Na2S) (BHT). 4.7 , ( ), , ( 40 60 ), -, . 4.

5、8 ( ) : 3 % 1,4- 0,5 % -2, 3 % - - - - () 1 % 10 % - (). - . . 4.9 4.9.1 -, - - Merck1), - . 90 % 100 %, (. 4.10.5). 4.9.2 -, (29502) = 430,7 /, 95 %. - (31523) = 472,7 / . 4.9.3 -, (28482) = 416,7 /, 90 %. 4.9.4 -, (28482) = 416,7 /, 90 %. 4.9.5 -, (27462) = 416,7 /, 90 %. 4.10 4.10.1 - 1 - (4.9.2)

6、, 10 , , 100 3, , - - - . 4.10.2 - 1 - (4.9.3), 10 , , 100 3, , - - - . 4.10.3 - 1 - (4.9.4), 10 , , 100 3, , - - - . 1) - CEN. EN 128222014 3 4.10.4 - 1 - (4.9.5), 10 , , 100 3, , - - - . 4.10.5 (4.10.1 4.10.4) (. 1) , (5.1). -, 10 3 50 C (5.2). , 10 3 . . , /3, -, -, - - : %11410EA (1) A ; %1 1E ,

7、 1. 1 %1 1E ( ), %1 1E - 292 76 11 - 296 89 11 - 298 91 11 - 298 87 11 - 292 255 ( ) %1 1E . %1 1E 6 8, , 14, 15. 4.11 4.11.1 - 10 3 - (4.10.1) 100 3 (, - -, - ). - 1 10 /3. . 4 C. 4.11.2 -, -, - - 10 3 (4.10) 100 3 (, - -, - - ). 1 10 /3. 4 C. EN 128222014 4 5 , , . 5.1 , , , 1 . 5.2 , . . 5.3 , , , ( 295 , 330 ) . , 292 . . . 5.4 - , 4,0 4,6 , 100 250 , 5 . . . . , , LiChrosorb Si 60, Spherisorb Si, Hypersil Si Lichrospher 100 DIOL1). , 18, 5 , 4,0 4,6 , 100 250 . - Spherisorb ODS Hypersil ODS1). - - -. , , - -. 5.5 ,