GOST EN 13585-2013 Foodstuffs Determination of fumonisins B1 and B2 in maize HPLC method with solid phase extraction clean-up《食品 玉蜀黍中伏马菌素B1和B2的测定 固相萃取净化HPLC方法》.pdf

1、 , () INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) EN 13585 2013 1 2 (EN 13585:2001, IDT) EN 13585-2013 II , 1.0 92 . 1.22009 . , . , , , 1 - ( ) - ( ) , 4 2 ( 335) 3 , ( 27 2013 . 57-) : ( 3166) 00497 ( 3166) 00497 AM BY KG MD - RU UZ 4 N 13585:2001 Foodstuffs. Determin

2、ation of fumonisins B1and B2in maize. HPLC method with solid phase extraction clean-up ( . 1 2 . ). (en). , , . (IDT) 5 31 2013 . 1294- EN 13585-2013 1 2015 . 6 EN 135852013 III , . () . , , 2014 , EN 135852013 1 1 2 Foodstuffs. Determination of fumonisins B1and B2in maize. HPLC method with solid ph

3、ase extraction clean-up 20150701 1 1 2 (). , 1. 1 405 6732 / 2 152 2619 /. (, , ), . 2 . . , . EN ISO 3696 Water for analytical laboratory use Specification and test methods (ISO 3696:1987) ( . ). 3 . . , , 2- . () - . , . . . , , 5 %. 4 4.1 N ISO 3696. . EN 135852013 2 4.2 . 4.3 - (3) = 75 % 75 4.2

4、 25 . 4.4 - (3N) = 50 % 50 50 . 4.5 (34) = 85 %. 4.6 (H3C) = 1 % 99 4.2 1 . 4.7 . 4.8 2-. 4.9 , (NaH2PO4 2H2O) = 0,1 /3 15,6 (NaH2PO4 2H2O) 1 3. 4.10 , (Na24O7 10H2O) = 0,1 /3 3,8 (Na24O7 10H2O) 100 3. 4.11 , c (NaOH) = 0,1 / 3 0,4 (NaOH) 100 3. 4.12 77 4.2 23 4.9. 3,35 . 4.5. 5.7. . 4.13 40 4.7 1 3

5、 4.2. 5 3 4.10 50 32- 4.8, . . 4.14 1 2, 1 2 - 4.4 250 /3. . 100 3 , 300 3- . 1 2 500 3 50 /3. , . (, 20, 50, 100 200 3) - 500 3. 4 , 6 . 5 , , . 5.1 : 5.1.1 , 1 3/, (), 10 3. 5.1.2 , - , , (DS), , : ; 150 ; 4,6 ; EN 135852013 3 5 ; , - . . 5.1.3 , 335 440 . 5.1.4 . 5.2 , . 5.3 . 5.4 , , , SAX Bond-

6、Elut1, 500 , . 5.5 , (). 5.6 (), , . 5.7 0,45 . 6 , , , . 7 , 1,0 . . 8 8.1 50 (, 250 3). 100 3- 4.3, 5.2 10000 -1. , , . 10 500 g. 5.3. , 4.11 5,8 6,5 . pH. 8.2 5.4 5.5. 5 3 4.2, 5 3- 4.3. 10 3 2 3/, 8 3- 4.3, 3 3 4.2, . . 10 3 4.6 2耀 1 3/, . 60 5.6. 1 3 4.2, , , . . 4 , . 8.3 8.3.1 25 3 1 2 4.14 225 3 4.13. 1Bond-Elut , . CEN . EN 135852013 4 , 5.1.2 10 3, 0,050 . , , , 3 . 5.1.3, 80 % . 8.3.2 , 8.2, 200 3 4.2. - 4.4. 25 3