GOST EN 14122-2013 Foodstuffs Determination of vitamin B1 by HPLC《食品 高效液相色谱分析 (HPLC) 法测定维生素B1》.pdf

1、 , () INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) N 14122 2013 1 (N 14122:2003, IDT) 2014 N 141222013 II , - 1.092 . - 1.22009 . -, . , , , 1 - - ( ) 2 ( 093) 3 , (- 5 2013 . 61-) : ( 3166) 00497 ( 3166) 00497 AM BY KZ KG MD RU UZ - 4 04 2014 . 77- N 141222013 1 2015 .

2、5 N 14122:2003 Foodstuffs. Determination of vitamin B1 by HPLC ( . 1 - ). CEN/TC 275 . , DIN. (ge). , - , - . - . (IDT)6 25999-83 3 4 N 141222013 III - , . () - - . , - , 2014 -, - N 141222013 IV 1 . 1 2 1 3 . 1 4 . 1 5 . 4 6 . 5 7 . 7 8 . 8 9 8 () . 9 () 11 () 14 () 17 . 18 N 141222013 1 1 Foodstuf

3、fs. Determination of vitamin B1by HPLC 20150701 - , . . . 1 1 (). 1- , . 2 , - . - . EN ISO 3696:1995 Water for analytical laboratory use Specification and test methods (ISO 3696:1987) ( . ) 3 , , 1 6. 4 4.1 - N ISO 3696 . N 141222013 2 4.2 4.2.1 99,8 %. 4.2.2 , (CH3COOH) = 0,02 /3. 4.2.3 () 98 %. 4

4、.2.4 99,8 %. 4.2.5 36 %. 4.2.6 , (HCl) = 0,1 /3. 4.2.7 , c(H2SO4) = 0,05 /3. 4.2.8 99 %. 4.2.9 , (NaOH) = 150 /3. 4.2.10 , (NaOH) = 200 /3. 4.2.11 (III) 99 %. 4.2.12 (III), (K3Fe(CN)6) = 10 /3. 4.2.13 (III), (K3Fe(CN)6) = 0,4 /3 (III) (. 4.2.12) 2 3 - (. 4.2.9) 50 3. . 4.2.14 (III), (K3Fe(CN)6) = 0,

5、5 /3 (III) (. 4.2.12) 2,5 3 - (. 4.2.10) 50 3. 4.2.15 , . -*.4.2.16 , c(CH3COONa 32) = 2,5 /3. 4.2.17 , c(CH3COONa 32) = 0,5 /3. 4.2.18 . , - , 10 % 50 %, - . 4.2.19 ( = 3,5) (24) = 9,0 /3. 4.2.20 98 %. 4.2.21 98 %. 4.2.22 ( = 4) (3) = 50 /3. 4.3 4.3.1 , - . 4.4.4. 4.3.2 (C12H17ClN4OS HCl) 99 %. 4.3

6、.3 (C12H17ClN4O4S) 98 %. 4.3.4 () (C12H19ClN4O72S) 98 %. *- 00040 , Platz and Bauer, Waterbury,CT 06708, . . . N 141222013 3 4.4 4.4.1 , (C12H17ClN4OS HCl) = 0,1 /3 (. 4.3.2) , , 10 , - , , , 100 3 -, , (. 4.2.6). 4 . 4.4.2 , (C12H17ClN4O4S) = 0,1 /3 10 (. 4.3.3), , 100 3 , , (. 4.2.6). 20 . 4.4.3 ,

7、 (C12H19ClN4O72S) = 0,1 /3 10 (. 4.3.4), , 100 3 , , (. 4.2.6). 4.4.4 100 3 10 3 - (. 4.4.1), (. 4.2.6). (. 5.2) 1 247 , - . (. 4.2.6). , /3, 4247 10 10,421= (1) 247 247 , ; 104 - ; 10 , ; 421 1%1A - 0,1 /37, - 10 /3. 4.5 4.5.1 1 10 /3 1 10 3 - (. 4.4.1) 100 3. - (. 4.2.6). 4 1 . 4.5.2 1 10 /3 1 10

8、3 - (. 4.4.2) 100 3. - (. 4.2.6). 4 1 . N 141222013 4 4.5.2 1 10 /3 1 10 3 - (. 4.4.3) 100 3. - (. 4.2.6). 4 1 . 5 5.1 , -, , . 5.2 , - . 5.3 , . 5.4 , , , , - , , , 366 420 , , , . 5.5 5.5.1 , - , , . - - . - .*5.5.2 100 250 , 4,0 4,6 , -* 5 . 5.5.3 . 100 250 , 4,0 4,6 , -* 5 .5.6 - , , 0,45 , . *