GOST EN 14132-2013 Foodstuffs Determination of ochratoxin A in barley and roasted coffee HPLC method with immunoaffinity column cleanup《食品 大麦和焙炒咖啡中赭曲霉素A的测定 免疫亲和柱层净化的高效液相色谱 (HPLC) 法.pdf

1、 . (EN 14132:2009, IDT) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)EN 141322013, - 1.092 . 1.22009 . -, . , , 1 - ( ) - ( -) , 52 (-)3 , ( 14 2013 . 44) : ( 3166) 00497 ( 3166) 00497 AMBYKZKGRUUZ 4 22 2013 . 1916- EN 141322013 1 2015 .5 N 14132:2009Foodstuffs. Determ

2、ination of ochratoxin A in barley and roasted coffee. HPLC method with immunoaffinitycolumn cleanup ( . . ). (en). , , - . (IDT)6 II EN 141322013 -, - . () . , - - , 2015 -, III EN 1413220131 .12 13 .14 15 36 47 58 69 .610 .711 8 () 9 () 1111IV EN 141322013 . Foodstuffs. Determination of ochratoxin

3、A in barley and roasted coffee.HPLC method with immunoaffinity column cleanup1 () . - 0,1 4,5 / 0,2 5,5 /.2 - . . -, .EN ISO 3696 Water for analytical laboratory use Specification and test methods (ISO 3696:1987)( . )3 - , . . , - , . - -.4 4.1 N ISO 3696:1995. . , .4.2 .4.3 .1 EN 141322013 20150701

4、4.4 .4.5 .4.6 , c114 (NaOH) = 8,0 /3 8 900 3, 13.4.7 - 8,0 4.2, 1,2 4.3,0,2 4.4 0,2 4.5 900 3. - 7,4 . 4.13, 1 3. - .4.8 , c114 (NaHCO3)=30/3 30 900 3 1 3. .4.9 w (3) = 98 %.4.10 .4.11 .4.12 .4.13 99 4.12 4.9.4.14 4.11 .4.15 4.10 - 4.8.4.16 30 4.10 70 - 4.9.4.17 102 96 4.11 - 4.9. - 0,2 5.12. .4.18

5、1 20 4.10 80 - 4.8.4.19 2 - c114 (Na2CO3) = 1 /100 3 1 90 3 100 3, .4.20 4.10 93 .4.21 , 1 - 4.13, 20 30 /3. - 300 370 5 1 5.14. 4.13. , -2 EN 141322013. , c114ota,/3, c114ota=AMbmaxc215100c101,(1)max (c108max= 333); , / (=403,8 /);c101 4.13,2/ (c101 = 544 2/);b , . 18 , - .4.22 , 4.21 4.13 10 /3. 4

6、. .4.23 , 200 3 -10/34.22.1380034.13, 2 /3. 100 3 5.2, , 10 3 4.16, - 0,2 5.12. - 20 /3. .4.24 , 100 3 -10 /3 4.22. 2 3 1,9 3 4.13. - 500 /3.4.25 , - , , 100 . 100 , 6.1.2, 85%.4.26 , - , 500 , - 3 3, , - . 100 . 85 % 100 , 4.15.5 , , .5.1 , 0,01 .5.2 10 3 - . , 3 EN 141322013 1 . (, ), , .5.3 1 3, , 20000 1.5.4 200 3,153 -.5.5 , ().5.6 .5.7 , 10 18 3/.5.8 .5.9 , 1300 g 4 .5.10 50 3.5.11 20 25 .5.12 0,2 , 25 .5.13 5.13.1 , 100 3.5.13.2 , , - 1 3/.5.13.3 , - , - (DS), . 10 % .