GOST EN 15452-2013 Fertilizers Determination of chelating agents Determination of iron chelated by EDDHA by ion pair chromatography《肥料 螯合剂的测定 反相HPLC法测定o p-EDDHA螯合的铁》.pdf

1、 , ( )INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC) EN 154522013 . , o,p-EDDHA HPLC(EN 15452:2008, IDT) 2014 EN 15452 2013 II, 1.0-92 . 1.2-2009 . , - . , , -, 1 - , , ( ) , 42 527 3 , ( 59- 27 2013 .) : ( 3166) 004-97 ( 3166) 004- 97 AZBYKGMDRUUZ -4 EN 15452:2008 Fertil-i

2、zers - Determination of c elatin a ents - Determination of iron c elate o,p-EDD A re erse ase (. . , o,p-EDD A, ). EN 260 - . (en). , , , , - . . - (ID )5 08 2013 . 1435- EN 15452 201 01 2015 .6 EN 15452 2013 III , . ( ) . , , 2014 -, 1 EN 15452 2013 2015 01 011 - EDDHA (,-EDDHA) . , . .1 , , - EDDH

3、A (,-EDDHA) .2 o,o-EDDHA o,p-EDDHA , . - .1 3 ,-EDDHA o,p-EDDHA . , -EDDHA d/l ( ), o,p-EDDHA d/l . .4 ,-EDDHA. o,p-EDDHA o,p-EDDHA, o,p-EDDHA.2 . - ( ).EN 1482-2 Fertilizers and liming materials Sampling and sample preparation Part 2: Sample prepa-ration ( . . 2. )3 () - 277 . - - ( =0,015 /3, 3,0)

4、, . ,-EDDHA ,-EDDHA -. , ,-EDDHA (,-Fe) .4 . EDTA, HEDTA, DTPA EDDHMA -. . , EDDHSA, - Fertilizers. Determination of chelating agents. Determination of iron chelated by EDDHSA by ion pair chromatography EN 15452 2013 25 5.1 ) .b) , , -, 1 4.c) - () 99 % -.5.2 , c(NaOH) = 0,1 /3: , - , 4 NaOH 1 3, .5

5、.3 , c(HCl) = 4 /3: 395 (37 %) 1000 3 .5.4 , c(HCl) = 0,1 /3: 25 3 (5.3) 1000 3 .5.5 (III) , (Fe) = 4200 /3: 3,04 (III) Fe(NO3)3.9H2O 80 3 , 1 3 (5.3), 100 3, . , Fe - (4200 100) /3, (, S ). .5.6 o,p-Fe-EDDHA, (o,p-Fe) = 100 /3: 100 3 0,1 (64,5 1,0) 100/P - o,p-H4-EDDHA, - Fe (III) 6 ( ), o,p-H4-EDD

6、HA (mco,p - . 5.1,). 5 3 NaOH (5.2) . 35 3 2,5 3 (III) (5.5). 3,0, NaOH (5.2) HCl (5.4). 100 3. .5.7 : 1,0 800 3 . 3,0, HCl (5.3 5.4). 1000 3. - . 0,45 . 915 3 ( 3,0) 85 3 . - . . , .6 6.1 , : 6.2 .6.3 , :a) , 1,0 /;b) 20 ;c) C18 (ODS-2); 250 4,6 ID; d p = 5 *);d) C18 (ODS-2) ( );e) UV/VIS- 277 ;f)

7、.6.4 , , 0,45 .* Spher orb ODS-2 a er All phere ODS-2 All ech , - . - CEN . EN 15452 2013 37 , . - 1. EN 1482-2. - . 1 .8 8.1 0,1 (250 10) (ms) 250 3. 200 3 30 . 250 3. . - o,p-Fe 0,5 % 5 %, - . .8.2 o,p-Fe-EDDHA V (3) (5.6) (. 1) 50 3. . o,p-EDDHA , - 1. o,p-EDDHA , /3, ,-Fe = d/l-1 + d/l-2 , (1) ,

8、d/l 1 d/l 2 55,8470,5 50 0,1 100 360,35co pmV P = = , (2) V - , 3;mco,p o,p-H4-EDDHA, , ;P - , %. , o,p-EDDHA - . 1 - V, 3o,p-EDDHAd/l -1 d/l -2Fe, /3 Fe, /31 2,5 2,5 2,52 5 5,0 5,03 10 10,0 10,04 15 15,0 15,05 20 20,0 20,06 25 25,0 25,08.3 - (6.4) 0,45 . (8.2) (6.3). o,p-Fe-EDDHA (d/l-1 d/l-2) . EN

9、 15452 2013 4 Fe (/3), - . (8.1). - ( .1, ). . - (/3) , .9 Fe, o,p-EDDHA, w, %, -, / 1 , / 2, 25mo p d l o p d lo p Fes += , o,p-d/l-1 Fe, (d/l-1) o,p-EDDHA, /3;o,p-d/l-2 Fe, (d/l-2) o,p-EDDHA, /3;ms , , .10 10.1 2005 . 12 4 . B. 2.10.2 , - , , 5 % r, 2. 10.3 , - , 5 % R, 2. 2 , o,p-Fe, % p n r R1 2,17 10 2 0,037 0,252 1,33 10 2 0,051 0,293 0,47 9 2 0,047 0,164 0,15 8 2 0,0070 0,25 - :o,p-Fe Fe, o,p-EDDHA;p ;n .r , /100 o,p-Fe;R , /100 o,p-Fe.(3) EN 15452 2013 5 - , Fe, - ,-EDDHA, , , 3 .11 :a)