GOST ISO 10272-1-2013 Microbiology of food and animal feeding stuffs Methods for detection and enumeration of Campylobacter spp Part 1 Detection method《食品和动物饲料的微生物学 弯曲杆菌属检测和计数方法 第1.pdf

1、 Campylobacter spp. 1 (ISO 10272-1:2006, IDT) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)ISO 10272-12013, - 1.092 . - 1.22009 . , . , , -, 1 - ( )2 3 , (- 7 2013 . 43) : ( 3166) 00497 ( 3166) 00497 KZKGRUTJUZ 4 27 2013 . 227- ISO 10272-12013 12014 .5 ISO 10272-1:2006

2、 Microbiology offood and animal feeding stuffs Horizontal method for detection and enumeration of Campylobacterspp. Part 1: Detection method ( . - Campylobacter spp. 1. ). SC 9 ISO/TC 34 (ISO). (en). , - , , , (-) . (IDT). 10272-120106 - , - . () - . , , 2013 -, II ISO 10272-120131 12 13 24 25 26 .3

3、7 .38 49 410 6 A () .7 B () 8 A () .12 13III ISO 10272-12013 Campylobacter spp. 1 Microbiology of food and animal feeding stuffs. ethods for detection and enumeration of Campylobacter spp.Part 1. Detection method 201407011 Campylobacter spp. , . - .2 :ISO 6887 ( ) Microbiology of food and animal fee

4、ding stuffs Preparation of test samples,initial suspension and decimal dilutions for microbiological examination ( . , - )ISO 7218 Microbiology of food and animal feeding stuffs General requirements and guidance formicrobiological examinations ( . - )ISO 8261 Milk and milk products. General guidance

5、 for the preparation of tests samples, initialsuspensions and decimal dilutions for microbiological examination ( . , - )ISO/TS 11133-1 Microbiology of food and animal feeding stuffs Guidelines on preparation andproduction of culture media Part 1: General guidelines on quality assurance for the prep

6、aration of culturemedia in the laboratory ( . - . 1. )ISO/TS 11133-2 Microbiology of food and animal feeding stuffs Guidelines on preparation andproduction of culture media Part 2: Practical guidelines on performance testing of culture media (- . - . 2: )1 ISO 10272-12013 3 :3.1 Campylobacter (Campy

7、lobacter): , , 41,5 C, 25 C, , - , . Campylobacter jejuni Campylobactercoli. (Campylobacter lari, Campylobacter upsaliensis ).3.2 Campylobacter (detection of Campylobacter): - - .4 4.1 Campylobacter A.4.2 ( ) . 37 C 46 41,5 C (44 c177 4) .4.3 , 4.2, ( ):- , ( mCCD);- , , - mCCD. 41,5 C (44 c177 4) ,

8、 - Campylobacter.4.4 , Campylobacter, - - , . Campylobacter .5 5.1 , Campylobacter ISO 7218, ISO/TS 11133-1 ISO/TS 11133-2. - B.5.2 : . B.1.5.3 : , ( mCCD). B.2.5.4 5.4.1 . B.3.5.4.2 . B.4.2 ISO 10272-120135.4.3 . B.5.5.4.4 , 3 % ( ).5.4.5 . B.6.5.4.6 -. B.7.5.4.7 (30 ) (30 ).5.4.8 . B.8.6 ISO 7218.

9、6.1 ( ) (-) ISO 7218.6.2 , , 37 55 .6.3 , (41,5 c177 1) .6.4 , (25 c177 1) (37 c177 1) , , (25 c177 1) (37 c177 1) .6.5 , 47 50 C.6.6 pH-, 0,1 25 .6.7 18 c180 180 9 c180 180 , 13 c180 75 , / .6.8 , , 90100 .6.9 , , -1103, 0,1 3, .6.10 , .6.11 -, - - 3 .- (. 9.4.6).6.12 , , , .6.13 , ( - Campylobacte

10、r).6.14 (5 c177 2) %, (10 c177 3) %, c163 10 %, -. , / 350 3, .1 ; , , . - .2 , , 2 .7 . - . , . - , - .3 ISO 10272-12013 , Campylobacter spp. , - (3 c177 2) . . .8 . , , .9 (. A)9.1 , ( ) - (5.2), -/ 1:10(/ /). .9.2 (9.1) (6.14) 37 46 , 41,5 (44 c177 4) .9.3 9.3.1 , (9.2), (6.11) - , mCCD (5.3). Campylobacter. , , - mCCD. , , Skirrow, Karmali Preston (. ).9.3.2 (9.3.1) 41,5 (6.14).9.3.3 (44 c177 4) / Campylobacter. mCCD , , . . .9.4 Campylobacter9.4.1 , - , 9.4.29.4.6.9.4.2